Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,811,028 G→A 13.1% A573V (GCA→GTA)  uvrA ← ATPase and DNA damage recognition protein of nucleotide excision repair excinuclease UvrABC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,811,0280GA13.1% 210.0 / 20.4 76A573V (GCA→GTA) uvrAATPase and DNA damage recognition protein of nucleotide excision repair excinuclease UvrABC
Reads supporting (aligned to +/- strand):  ref base G (30/36);  new base A (6/4);  total (36/40)
Fisher's exact test for biased strand distribution p-value = 5.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.71e-01

GCGTTTGCCGCTCATGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAA  >  minE/2810958‑2811094
                                                                      |                                                                  
gCGTTTGCCGCTCATGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTg                                                                    >  1:2821435/1‑71 (MQ=255)
  gTTTGCCGCTCATGTACTTCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCg                                                                  <  1:1671840/71‑1 (MQ=255)
  gTTTGCCGCTCATGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCg                                                                  <  1:2958654/71‑1 (MQ=255)
      gCCGCTCATGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGAcc                                                               <  1:3164834/70‑1 (MQ=255)
      gCCGCTCATGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGAcc                                                               <  1:2810563/70‑1 (MQ=255)
        cGCTCATGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACt                                                            >  1:69885/1‑71 (MQ=255)
         gCTCATGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACtt                                                           <  1:944687/71‑1 (MQ=255)
          cTCATGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACtt                                                           <  1:700441/70‑1 (MQ=255)
             aTGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTc                                                          <  1:3133535/68‑1 (MQ=255)
              tGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGAc                                                                <  1:2249868/61‑1 (MQ=255)
                  cTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGt                                                  <  1:985600/71‑1 (MQ=255)
                     cccGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTg                                                 <  1:1528356/69‑1 (MQ=255)
                     cccGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTACGACCACTTCACCGCCGTg                                                 <  1:4376664/69‑1 (MQ=255)
                     cccGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTACGACCACTTCACCGCCGTg                                                 <  1:3960458/69‑1 (MQ=255)
                     cccGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTACGACCACTTCACCGCCGTg                                                 <  1:356380/69‑1 (MQ=255)
                     cccGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTACGACCACTTCACCGCCGTGaa                                               >  1:4706847/1‑71 (MQ=255)
                     cccGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTACGACCACTTCACCGCCGTGaa                                               >  1:1552059/1‑71 (MQ=255)
                     cccGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTACGACCACTTCACCGCCGTGaa                                               >  1:2548150/1‑71 (MQ=255)
                         gTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTg                                                                    <  1:710204/46‑1 (MQ=255)
                         gTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTg                                                                    <  1:3153264/46‑1 (MQ=255)
                         gTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTg                                                                    <  1:3143012/46‑1 (MQ=255)
                         gTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTg                                                                    <  1:64300/46‑1 (MQ=255)
                         gTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTACGACCACTTCACCGCCGTg                                                 <  1:508406/65‑1 (MQ=255)
                               gACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGcc                                     >  1:611635/1‑71 (MQ=255)
                                aCTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCgc                                                                 >  1:2328244/1‑41 (MQ=255)
                                aCTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCAc                                                             >  1:4052554/1‑46 (MQ=255)
                                aCTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGaa                                               >  1:4364087/1‑60 (MQ=255)
                                aCTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGaa                                               >  1:4496908/1‑60 (MQ=255)
                                aCTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGaa                                               >  1:4146945/1‑60 (MQ=255)
                                aCTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGccc                                    >  1:4406767/1‑71 (MQ=255)
                                 cTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCg                                   <  1:2342041/71‑1 (MQ=255)
                                 cTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCg                                   <  1:1976424/71‑1 (MQ=255)
                                 cTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCg                                   <  1:3667529/71‑1 (MQ=255)
                                   ccGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGAc                                                                <  1:2202489/40‑1 (MQ=255)
                                    cGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCa                                                              >  1:685526/1‑41 (MQ=255)
                                    cGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCTTGAACACCTGCGCCCGGGc                                >  1:3558788/1‑71 (MQ=255)
                                    cGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCggg                                 <  1:1635068/70‑1 (MQ=255)
                                    cGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGc                                >  1:4694610/1‑71 (MQ=255)
                                    cGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGc                                >  1:84760/1‑71 (MQ=255)
                                    cGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGc                                >  1:3666394/1‑71 (MQ=255)
                                         ccGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATg                           >  1:285668/1‑71 (MQ=255)
                                             cATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGa                       >  1:1811450/1‑71 (MQ=255)
                                               tAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCaa                             <  1:381765/63‑1 (MQ=255)
                                                 aTCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATca                    <  1:2273588/70‑1 (MQ=255)
                                                 aTCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATca                    <  1:3930825/70‑1 (MQ=255)
                                                  tCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAacac                                            >  1:4510782/1‑45 (MQ=255)
                                                   gcgttCCGGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACAt                 <  1:2095690/68‑1 (MQ=255)
                                                   gcgttCCGGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACAt                 <  1:49177/68‑1 (MQ=255)
                                                   gcgttCCGGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACAt                 <  1:1172885/68‑1 (MQ=255)
                                                   gcgttCCGGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACAt                 <  1:1796270/68‑1 (MQ=255)
                                                    gCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGaa                                               >  1:337159/1‑40 (MQ=255)
                                                    gCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGaa                                               >  1:2544100/1‑40 (MQ=255)
                                                    gCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGaa                                               >  1:2087801/1‑40 (MQ=255)
                                                    gCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGaa                                               >  1:159636/1‑40 (MQ=255)
                                                    gCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGa                                                >  1:2294396/1‑39 (MQ=255)
                                                    gCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATg                <  1:2058151/71‑1 (MQ=255)
                                                    gCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATg                <  1:3586600/71‑1 (MQ=255)
                                                     cTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATgg               <  1:1444641/71‑1 (MQ=255)
                                                        ccAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCATATGGTc             <  1:610894/70‑1 (MQ=255)
                                                        ccAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTc             <  1:3458192/70‑1 (MQ=255)
                                                        ccAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTc             <  1:633815/70‑1 (MQ=255)
                                                        ccAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTc             <  1:2639407/70‑1 (MQ=255)
                                                         cAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCa            <  1:3724831/70‑1 (MQ=255)
                                                         cAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAg           <  1:200863/71‑1 (MQ=255)
                                                         cAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAg           >  1:2714584/1‑71 (MQ=255)
                                                           gCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCa                              <  1:1343331/50‑1 (MQ=255)
                                                            cGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTgcg                                       <  1:4066906/40‑1 (MQ=255)
                                                              gACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGt                          >  1:1755507/1‑51 (MQ=255)
                                                              gACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAgcggcg      <  1:2876388/71‑1 (MQ=255)
                                                               aCCTTCTGCGACCACTTCACCGCCGTGAACACCTTCGCCCGGGCCAATGTCGATcac                   >  1:737291/1‑57 (MQ=255)
                                                               aCCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATcac                   >  1:4045459/1‑57 (MQ=255)
                                                               aCCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATcac                   >  1:4239326/1‑57 (MQ=255)
                                                                 cTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGcgcg    >  1:1821695/1‑70 (MQ=255)
                                                                 cTTCTACGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGcgcg    >  1:4003757/1‑70 (MQ=255)
                                                                 cTTCTACGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGcgcg    >  1:2520869/1‑70 (MQ=255)
                                                                 cTTCTACGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGcgcg    >  1:2515282/1‑70 (MQ=255)
                                                                   tCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAg           >  1:726747/1‑61 (MQ=255)
                                                                   tCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAg           >  1:1728319/1‑61 (MQ=255)
                                                                    cTGCGACCACTTCACCGCCGTGAACACCTGCGCCCggg                                 >  1:357440/1‑38 (MQ=255)
                                                                    cTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGaa  <  1:3729604/69‑1 (MQ=255)
                                                                    cTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGaa  <  1:618551/69‑1 (MQ=255)
                                                                      |                                                                  
GCGTTTGCCGCTCATGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAA  >  minE/2810958‑2811094

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: