Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | minE | 2,811,028 | G→A | 13.1% | A573V (GCA→GTA) | uvrA ← | ATPase and DNA damage recognition protein of nucleotide excision repair excinuclease UvrABC |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | minE | 2,811,028 | 0 | G | A | 13.1% | 210.0 / 20.4 | 76 | A573V (GCA→GTA) | uvrA | ATPase and DNA damage recognition protein of nucleotide excision repair excinuclease UvrABC |
Reads supporting (aligned to +/- strand): ref base G (30/36); new base A (6/4); total (36/40) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 5.03e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.71e-01 |
GCGTTTGCCGCTCATGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAA > minE/2810958‑2811094 | gCGTTTGCCGCTCATGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTg > 1:2821435/1‑71 (MQ=255) gTTTGCCGCTCATGTACTTCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCg < 1:1671840/71‑1 (MQ=255) gTTTGCCGCTCATGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCg < 1:2958654/71‑1 (MQ=255) gCCGCTCATGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGAcc < 1:3164834/70‑1 (MQ=255) gCCGCTCATGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGAcc < 1:2810563/70‑1 (MQ=255) cGCTCATGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACt > 1:69885/1‑71 (MQ=255) gCTCATGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACtt < 1:944687/71‑1 (MQ=255) cTCATGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACtt < 1:700441/70‑1 (MQ=255) aTGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTc < 1:3133535/68‑1 (MQ=255) tGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGAc < 1:2249868/61‑1 (MQ=255) cTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGt < 1:985600/71‑1 (MQ=255) cccGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTg < 1:1528356/69‑1 (MQ=255) cccGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTACGACCACTTCACCGCCGTg < 1:4376664/69‑1 (MQ=255) cccGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTACGACCACTTCACCGCCGTg < 1:3960458/69‑1 (MQ=255) cccGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTACGACCACTTCACCGCCGTg < 1:356380/69‑1 (MQ=255) cccGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTACGACCACTTCACCGCCGTGaa > 1:4706847/1‑71 (MQ=255) cccGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTACGACCACTTCACCGCCGTGaa > 1:1552059/1‑71 (MQ=255) cccGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTACGACCACTTCACCGCCGTGaa > 1:2548150/1‑71 (MQ=255) gTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTg < 1:710204/46‑1 (MQ=255) gTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTg < 1:3153264/46‑1 (MQ=255) gTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTg < 1:3143012/46‑1 (MQ=255) gTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTg < 1:64300/46‑1 (MQ=255) gTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTACGACCACTTCACCGCCGTg < 1:508406/65‑1 (MQ=255) gACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGcc > 1:611635/1‑71 (MQ=255) aCTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCgc > 1:2328244/1‑41 (MQ=255) aCTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCAc > 1:4052554/1‑46 (MQ=255) aCTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGaa > 1:4364087/1‑60 (MQ=255) aCTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGaa > 1:4496908/1‑60 (MQ=255) aCTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGaa > 1:4146945/1‑60 (MQ=255) aCTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGccc > 1:4406767/1‑71 (MQ=255) cTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCg < 1:2342041/71‑1 (MQ=255) cTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCg < 1:1976424/71‑1 (MQ=255) cTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCg < 1:3667529/71‑1 (MQ=255) ccGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGAc < 1:2202489/40‑1 (MQ=255) cGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCa > 1:685526/1‑41 (MQ=255) cGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCTTGAACACCTGCGCCCGGGc > 1:3558788/1‑71 (MQ=255) cGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCggg < 1:1635068/70‑1 (MQ=255) cGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGc > 1:4694610/1‑71 (MQ=255) cGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGc > 1:84760/1‑71 (MQ=255) cGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGc > 1:3666394/1‑71 (MQ=255) ccGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATg > 1:285668/1‑71 (MQ=255) cATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGa > 1:1811450/1‑71 (MQ=255) tAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCaa < 1:381765/63‑1 (MQ=255) aTCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATca < 1:2273588/70‑1 (MQ=255) aTCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATca < 1:3930825/70‑1 (MQ=255) tCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAacac > 1:4510782/1‑45 (MQ=255) gcgttCCGGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACAt < 1:2095690/68‑1 (MQ=255) gcgttCCGGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACAt < 1:49177/68‑1 (MQ=255) gcgttCCGGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACAt < 1:1172885/68‑1 (MQ=255) gcgttCCGGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACAt < 1:1796270/68‑1 (MQ=255) gCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGaa > 1:337159/1‑40 (MQ=255) gCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGaa > 1:2544100/1‑40 (MQ=255) gCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGaa > 1:2087801/1‑40 (MQ=255) gCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGaa > 1:159636/1‑40 (MQ=255) gCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGa > 1:2294396/1‑39 (MQ=255) gCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATg < 1:2058151/71‑1 (MQ=255) gCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATg < 1:3586600/71‑1 (MQ=255) cTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATgg < 1:1444641/71‑1 (MQ=255) ccAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCATATGGTc < 1:610894/70‑1 (MQ=255) ccAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTc < 1:3458192/70‑1 (MQ=255) ccAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTc < 1:633815/70‑1 (MQ=255) ccAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTc < 1:2639407/70‑1 (MQ=255) cAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCa < 1:3724831/70‑1 (MQ=255) cAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAg < 1:200863/71‑1 (MQ=255) cAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAg > 1:2714584/1‑71 (MQ=255) gCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCa < 1:1343331/50‑1 (MQ=255) cGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTgcg < 1:4066906/40‑1 (MQ=255) gACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGt > 1:1755507/1‑51 (MQ=255) gACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAgcggcg < 1:2876388/71‑1 (MQ=255) aCCTTCTGCGACCACTTCACCGCCGTGAACACCTTCGCCCGGGCCAATGTCGATcac > 1:737291/1‑57 (MQ=255) aCCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATcac > 1:4045459/1‑57 (MQ=255) aCCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATcac > 1:4239326/1‑57 (MQ=255) cTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGcgcg > 1:1821695/1‑70 (MQ=255) cTTCTACGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGcgcg > 1:4003757/1‑70 (MQ=255) cTTCTACGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGcgcg > 1:2520869/1‑70 (MQ=255) cTTCTACGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGcgcg > 1:2515282/1‑70 (MQ=255) tCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAg > 1:726747/1‑61 (MQ=255) tCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAg > 1:1728319/1‑61 (MQ=255) cTGCGACCACTTCACCGCCGTGAACACCTGCGCCCggg > 1:357440/1‑38 (MQ=255) cTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGaa < 1:3729604/69‑1 (MQ=255) cTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGaa < 1:618551/69‑1 (MQ=255) | GCGTTTGCCGCTCATGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAA > minE/2810958‑2811094 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |