| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,892,177 | G→A | 12.6% | D278N (GAC→AAC) | ytfQ → | predicted sugar transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,892,177 | 0 | G | A | 12.6% | 242.2 / 21.6 | 87 | D278N (GAC→AAC) | ytfQ | predicted sugar transporter subunit |
| Reads supporting (aligned to +/- strand): ref base G (44/32); new base A (6/5); total (50/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.15e-01 | |||||||||||
AAAGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGTTAACCAAATCCACCCTTTA > minE/2892108‑2892247 | aaaGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGa > 1:610956/1‑71 (MQ=255) aaGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCg > 1:4627829/1‑69 (MQ=255) aaGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGa > 1:4278533/1‑70 (MQ=255) aaGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGa > 1:733270/1‑70 (MQ=255) aaGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGa > 1:3989326/1‑70 (MQ=255) aaGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGAc > 1:350493/1‑71 (MQ=255) aaGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGAc > 1:3285118/1‑71 (MQ=255) aaGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGAc > 1:515443/1‑71 (MQ=255) aaGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGAc > 1:1643416/1‑71 (MQ=255) gCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGAcg > 1:4703405/1‑70 (MQ=255) gatggatgGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTg < 1:3489938/70‑1 (MQ=255) atggatggCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCAACGCGCTg > 1:2391769/1‑69 (MQ=255) atggatggCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCAACGCGCTg > 1:532468/1‑69 (MQ=255) atggatggCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCAACGCGCTg > 1:1769379/1‑69 (MQ=255) atggatggCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCAACGCGCTg > 1:313426/1‑69 (MQ=255) atggatggCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCAACGCGCTg > 1:1549189/1‑69 (MQ=255) tggatggCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTg > 1:807567/1‑68 (MQ=255) ggatggCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAAGTCCCGCCTTCGACGCGCTGgag > 1:2969867/1‑70 (MQ=255) gatggCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGgaga < 1:2898732/70‑1 (MQ=255) gatggCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGgaga < 1:347481/70‑1 (MQ=255) tggCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGAcgc < 1:3789573/60‑1 (MQ=255) tggCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGAcgc < 1:4449095/60‑1 (MQ=255) tggCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGAcgc < 1:230630/60‑1 (MQ=255) tggCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGAcgc < 1:2170305/60‑1 (MQ=255) tggCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGaaa > 1:133106/1‑70 (MQ=255) tggCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGaaa > 1:2293657/1‑70 (MQ=255) tggCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGaaa > 1:2665994/1‑70 (MQ=255) tggCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGaaa > 1:543985/1‑70 (MQ=255) gcgaagcgaaCGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATa > 1:3042404/1‑70 (MQ=255) gcgaagcgaaCGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATAc < 1:2407072/71‑1 (MQ=255) aagcgaaCGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCAACGCGCTGGAGAAATACaaa < 1:4312439/71‑1 (MQ=255) gcgaaCGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACaaaa > 1:557110/1‑70 (MQ=255) gcgaaCGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACaaaa > 1:747872/1‑70 (MQ=255) cgaaCGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACaaaaaa < 1:3338313/71‑1 (MQ=255) gaaCGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGaa < 1:2465986/60‑1 (MQ=255) aaCGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGaaa > 1:4677351/1‑60 (MQ=255) gCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGACgg < 1:1750154/71‑1 (MQ=255) gCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGACgg < 1:1577527/71‑1 (MQ=255) ccAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAg < 1:3439561/66‑1 (MQ=255) ccAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAg < 1:4129331/66‑1 (MQ=255) ccAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAg < 1:4722187/66‑1 (MQ=255) ccAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAg < 1:166448/66‑1 (MQ=255) gtgtTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCAc > 1:3581391/1‑70 (MQ=255) ttGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACaaaaaa < 1:4207962/59‑1 (MQ=255) gAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAg > 1:3243045/1‑58 (MQ=255) gAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAg > 1:4227343/1‑58 (MQ=255) gAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGACgg > 1:3763214/1‑62 (MQ=255) cTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGa < 1:2633676/71‑1 (MQ=255) tGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGgaga < 1:3556524/42‑1 (MQ=255) tGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTg > 1:1519979/1‑69 (MQ=255) aCGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGaa < 1:4707404/69‑1 (MQ=255) gCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAACGACGGCTCCAt < 1:3018839/60‑1 (MQ=255) gCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGaa < 1:2670975/67‑1 (MQ=255) gCCGAATATGGCAGGTCCCGCCTTCAACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAg < 1:1860395/70‑1 (MQ=255) ccGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCt < 1:3496803/71‑1 (MQ=255) gAATATGGCAGGTCCCGCCTTCGACGCGCTGGGGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGa > 1:4592261/1‑71 (MQ=255) gAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGa > 1:4433466/1‑71 (MQ=255) gAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGa > 1:1119572/1‑71 (MQ=255) gAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGa > 1:822826/1‑71 (MQ=255) gAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGa > 1:2823237/1‑71 (MQ=255) aaTATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACaaaa < 1:705372/43‑1 (MQ=255) tatGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGt > 1:4757042/1‑71 (MQ=255) tGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGtt > 1:678880/1‑70 (MQ=255) tGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGtt > 1:2020452/1‑70 (MQ=255) tGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGtt < 1:247359/70‑1 (MQ=255) ggCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGtt < 1:2281236/69‑1 (MQ=255) gCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGa > 1:3469718/1‑41 (MQ=255) gCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGa > 1:3467987/1‑41 (MQ=255) cAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCa > 1:2130979/1‑45 (MQ=255) cAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGTTaa > 1:3140150/1‑69 (MQ=255) cAGGTCCCGCCTTCAACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGATAAc > 1:2456733/1‑70 (MQ=255) aGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGTTAACCa < 1:417264/71‑1 (MQ=255) gTCCCGCCTTCGACGCGCTTGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGTTAACCaa < 1:405908/70‑1 (MQ=255) gTCCCGCCTTCGACGCGCTGGAGAAATACaaaaaa < 1:1010086/35‑1 (MQ=255) gTCCCGCCTTCAACGCGCTGGAGAAATACaaaaaa < 1:4727935/35‑1 (MQ=255) gTCCCGCCTTCAACGCGCTGGAGAAATACaaaaaa < 1:3661263/35‑1 (MQ=255) gCCTTCGCCGCGCTGGAGAAATACAAAAAAGACGGCACAATGCCTGAAAAGCTGACGTTa > 1:697651/1‑60 (MQ=255) gCCTTCAACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGTTAACCAAATCCAc < 1:4646513/71‑1 (MQ=255) ttCGACGCGCTGGAGAAATACAAAAAAGACGGCACCa > 1:3192119/1‑37 (MQ=255) ttCGACGCGCTGGAGAAATACAAAAAAGACGGCACCa > 1:4044984/1‑37 (MQ=255) ttCGACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGTTa < 1:3106509/57‑1 (MQ=255) ttCGACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGTTa < 1:1594241/57‑1 (MQ=255) ttCGACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGTTAACCAAATCCACCCt > 1:1649899/1‑71 (MQ=255) tCGACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGTTAACCAAATCCAccc < 1:4333949/69‑1 (MQ=255) cGACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGTTAAc > 1:1931326/1‑57 (MQ=255) cGACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGTTAAc > 1:1586103/1‑57 (MQ=255) cGACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGTTAAc > 1:4249090/1‑57 (MQ=255) cGACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGTTAAc > 1:2905249/1‑57 (MQ=255) gACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGaa < 1:2676535/42‑1 (MQ=255) gACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGTTAACCAAATCCACCCttt < 1:425307/70‑1 (MQ=255) gACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGTTAACCAAATCCACCCTTTa < 1:708973/71‑1 (MQ=255) | AAAGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGTTAACCAAATCCACCCTTTA > minE/2892108‑2892247 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |