| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,948,260 | G→A | 14.3% | intergenic (‑20/+118) | yjjB ← / ← yjjP | conserved inner membrane protein/predicted inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,948,260 | 0 | G | A | 14.3% | 400.2 / 48.3 | 147 | intergenic (‑20/+118) | yjjB/yjjP | conserved inner membrane protein/predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (67/59); new base A (14/7); total (81/66) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.44e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 148 total aligned reads displayed.
ATAAAGGTTGACCACTCAATATTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAACACCATCGCAAAGCCGACCGCAGG > minE/2948194‑2948330 | aTAAAGGTTGACCACTCAATATTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGc > 1:2170750/1‑70 (MQ=255)aTAAAGGTTGACCACTCAATATTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGc > 1:1440043/1‑70 (MQ=255)aTAAAGGTTGACCACTCAATATTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGc > 1:2844114/1‑70 (MQ=255)aTAAAGGTTGACCACTCAATATTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGc > 1:1191324/1‑70 (MQ=255) aaGGTTGACCACTCAATATTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCAAGCCAag > 1:2785182/1‑71 (MQ=255) aGGTTGACCACTCAATATTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAagc < 1:4103240/71‑1 (MQ=255) aGGTTGACCACTCAATATTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAag > 1:69086/1‑70 (MQ=255) aGGTTGACCACTCAATATTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAag > 1:113064/1‑70 (MQ=255) aGGTTGACCACTCAATATTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAag > 1:1709755/1‑70 (MQ=255) ttGACCACTCAATATTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAagcagc < 1:2360825/71‑1 (MQ=255) ttGACCACTCAATATTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAagcagc < 1:2441330/71‑1 (MQ=255) aCTCAATATTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCacac < 1:3390058/71‑1 (MQ=255) aCTCAATATTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCAAGCCAAGCAGCGcaca > 1:1526874/1‑70 (MQ=255) aCTCAATATTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCAAGCCAAGCAGCGCacac > 1:1810760/1‑71 (MQ=255) cTCAATATTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCacac > 1:2929724/1‑70 (MQ=255) tatTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAagca < 1:2879346/57‑1 (MQ=255) tatTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCg > 1:4744657/1‑70 (MQ=255) ttCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGca > 1:3471072/1‑60 (MQ=255) ttCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGca > 1:2552483/1‑60 (MQ=255) ttCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACAcc > 1:1320433/1‑64 (MQ=255) ttCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACAcc > 1:2188746/1‑64 (MQ=255) ttCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTaa > 1:3929775/1‑71 (MQ=255) ttCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTaa < 1:93953/71‑1 (MQ=255) ttCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTaa < 1:1817201/71‑1 (MQ=255) ttCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTa < 1:4622698/70‑1 (MQ=255) ttCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTa < 1:1002263/70‑1 (MQ=255) ttCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTa < 1:3268624/70‑1 (MQ=255) tCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTa < 1:3320450/69‑1 (MQ=255) tCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCAAGCCAAGCAGCGCACACCAGCGTAAc > 1:2602424/1‑71 (MQ=255) cAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAAc < 1:4495578/70‑1 (MQ=255) aaCCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGc < 1:1122815/71‑1 (MQ=255) gCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCa > 1:3259100/1‑71 (MQ=255) cTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCa > 1:2997860/1‑70 (MQ=255) cTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCAc > 1:1517398/1‑71 (MQ=255) tGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACg > 1:2622193/1‑71 (MQ=255) tcatcaAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGt < 1:892580/56‑1 (MQ=255) tcatcaAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACggg > 1:1425906/1‑70 (MQ=255) atcaAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGt < 1:1240214/54‑1 (MQ=255) atcaAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGt < 1:370610/54‑1 (MQ=255) atcaAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGa < 1:2056532/69‑1 (MQ=255) atcaAGATCATTCGGGAACCATGACCTATCAAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAAc < 1:2691673/71‑1 (MQ=255) tcaAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCg < 1:4659104/52‑1 (MQ=255) tcaAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGt < 1:4007320/53‑1 (MQ=255) tcaAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGt < 1:790903/53‑1 (MQ=255) tcaAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACg < 1:2114302/71‑1 (MQ=255) tcaAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACg < 1:3593946/71‑1 (MQ=255) tcaAGATCATTCGGGAACCATGACCTATCAAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGaa < 1:446410/69‑1 (MQ=255) caAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACgg > 1:2800651/1‑65 (MQ=255) aaGATCATTCGGGAACCATGACCTATCGAGCCAagcag > 1:550169/1‑38 (MQ=255) aaGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTaa > 1:3525184/1‑53 (MQ=255) aaGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTaa > 1:2578425/1‑53 (MQ=255) aaGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTaa > 1:2510304/1‑53 (MQ=255) aaGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTaa > 1:2926087/1‑53 (MQ=255) aaGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTaa > 1:2998567/1‑53 (MQ=255) aaGATCATTCGGGAACCATGACCTATCAAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGtt > 1:3458161/1‑71 (MQ=255) gATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGcac < 1:4634102/41‑1 (MQ=255) gATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGa < 1:2328527/71‑1 (MQ=255) gATCATTCGGGAACCATGACCTATCAAGCCAagcagc > 1:3114636/1‑37 (MQ=255) gATCATTCGGGAACCATGACCTATCAAGCCAagcagc > 1:3719657/1‑37 (MQ=255) aTCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACg > 1:4187549/1‑52 (MQ=255) aTCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACg > 1:2059551/1‑52 (MQ=255) tCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCg < 1:509010/46‑1 (MQ=255) tCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCg < 1:4625000/46‑1 (MQ=255) tCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCg < 1:2988116/46‑1 (MQ=255) tCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCg < 1:1873206/46‑1 (MQ=255) tCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCg < 1:1882029/46‑1 (MQ=255) cATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGcc > 1:1698297/1‑52 (MQ=255) cATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGcc > 1:3168004/1‑52 (MQ=255) cATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGcc > 1:3146640/1‑52 (MQ=255) cATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAaca > 1:3628746/1‑71 (MQ=255) cATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAaca > 1:1996394/1‑71 (MQ=255) ttCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAACAcc < 1:1079692/71‑1 (MQ=255) cGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAAc > 1:2231953/1‑59 (MQ=255) cGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACg > 1:4679410/1‑60 (MQ=255) cGGGAACCATGACCTATCAAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGtt > 1:3522160/1‑62 (MQ=255) cGGGAACCATGACCTATCAAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGtt > 1:2032879/1‑62 (MQ=255) cGGGAACCATGACCTATCAAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGtt > 1:3918303/1‑62 (MQ=255) gggAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAacac < 1:3250544/67‑1 (MQ=255) gggAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAacac < 1:470724/67‑1 (MQ=255) gggAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAacac < 1:2139463/67‑1 (MQ=255) gggAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAacac < 1:3968832/67‑1 (MQ=255) ggAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAACAcc < 1:2814496/67‑1 (MQ=255) ggAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAACAcc < 1:1184766/67‑1 (MQ=255) ggAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAACAcc < 1:125735/67‑1 (MQ=255) ggAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAACAcc < 1:546739/67‑1 (MQ=255) ggAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAACAcc < 1:2915901/67‑1 (MQ=255) ggAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAACAcc < 1:2856463/67‑1 (MQ=255) gAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAACACCa > 1:1330792/1‑67 (MQ=255) aaCCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAACACCATCGCa < 1:3296464/71‑1 (MQ=255) ccATGACCTATCAAGCCAAGCAGCGCACACCAGCGTAACTCCCGCACGGGAACGTTGAACACCATCGCaaa < 1:3987802/71‑1 (MQ=255) tGACCTATCGAGCCAAGCCGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAACTCCATCg > 1:4180392/1‑64 (MQ=255) tGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAACACCATCGCAAAGcc > 1:2636720/1‑71 (MQ=255) aTCGAGCCAAGCAGCGCACACCAGCGTAACGCCCg > 1:1032286/1‑35 (MQ=255) aTCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAACACCATCGCAAAGCCGACCg > 1:309413/1‑70 (MQ=255) tCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACgg > 1:4368844/1‑39 (MQ=255) tCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACgg > 1:4038483/1‑39 (MQ=255) tCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACgg > 1:852983/1‑39 (MQ=255) tCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGt > 1:2118137/1‑45 (MQ=255) tCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGt > 1:858645/1‑45 (MQ=255) gAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAACACCATCGCAAAGCCGACCGCAgg < 1:4229510/71‑1 (MQ=255) | ATAAAGGTTGACCACTCAATATTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAACACCATCGCAAAGCCGACCGCAGG > minE/2948194‑2948330 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |