| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 415,949 | C→G | 5.0% | S257T (AGC→ACC) ‡ | ybeZ ← | hypothetical protein with nucleoside triphosphate hydrolase domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 415,949 | 0 | C | G | 5.0% | 334.3 / 5.0 | 99 | S257T (AGC→ACC) ‡ | ybeZ | hypothetical protein with nucleoside triphosphate hydrolase domain |
| Reads supporting (aligned to +/- strand): ref base C (53/41); new base G (3/2); total (56/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01 | |||||||||||
Only 100 of 111 total aligned reads displayed.
GATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGTGCGACTTCAA > minE/415879‑416018 | gATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGc > 1:760082/1‑71 (MQ=255)gATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGc > 1:3058134/1‑71 (MQ=255) tAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGctc > 1:3090465/1‑71 (MQ=255) tAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGctc > 1:2372690/1‑71 (MQ=255) aCCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGctct > 1:2930438/1‑70 (MQ=255) aCCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGctct > 1:2842279/1‑70 (MQ=255) aCCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGctct < 1:3918815/70‑1 (MQ=255) aCCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGctct < 1:1882918/70‑1 (MQ=255) cGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCtctc > 1:1323739/1‑69 (MQ=255) cGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCAt > 1:1093753/1‑71 (MQ=255) cGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCAt > 1:78793/1‑71 (MQ=255) cGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCAt > 1:731179/1‑71 (MQ=255) cGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTACATCTGTTCGATGGTGGTGTTCTGGCTCTCAt > 1:3778705/1‑71 (MQ=255) gCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATc > 1:1367924/1‑71 (MQ=255) cTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTTTTCGATGGTGGTGTTCTGGCTCTCATCg < 1:3951505/71‑1 (MQ=255) cTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATc > 1:3432625/1‑70 (MQ=255) cTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCGGATCg > 1:2705049/1‑71 (MQ=255) ttttGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATc > 1:3404131/1‑69 (MQ=255) ttGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCgag > 1:1800460/1‑70 (MQ=255) ttGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCgag > 1:280283/1‑70 (MQ=255) ttGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCgag > 1:4240540/1‑70 (MQ=255) tGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGaa > 1:1530854/1‑71 (MQ=255) gTTAAAACCGATACGGGTCATGAACATCTTCATCTGTTCGATGTTGGTGTTCTGGCTCTCAt > 1:3607507/1‑62 (MQ=255) tAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGAt > 1:4357761/1‑70 (MQ=255) aaaaCCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATa > 1:2623437/1‑70 (MQ=255) ccGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAAcgc > 1:2976042/1‑71 (MQ=255) cGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAAcgcg < 1:2875490/71‑1 (MQ=255) gATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGcgt < 1:2846761/71‑1 (MQ=255) cGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGcgtcgt < 1:4644514/70‑1 (MQ=255) ggTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCa < 1:179884/71‑1 (MQ=255) agtaaCATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGt < 1:2819391/68‑1 (MQ=255) aGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTGAGCGt < 1:534097/71‑1 (MQ=255) aGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGt < 1:3031544/71‑1 (MQ=255) aGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGt < 1:3891284/71‑1 (MQ=255) aGGAACATCTTCATCTGCTCGACGGTCGTGTTCTGGGCTTCGTCCAGGATCACGAACGCGTGGTTc > 1:3525706/1‑66 (MQ=255) aGGAACATCTTCATCTGCTCGACGGTCGTGTTCTGGGCTTCGTCCAGGATCACGAACGCGTGGTTCAgg > 1:1431861/1‑68 (MQ=255) ggAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGt < 1:370660/70‑1 (MQ=255) gAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTa > 1:4744366/1‑70 (MQ=255) gAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTa > 1:3005192/1‑70 (MQ=255) gAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTAc > 1:3465459/1‑71 (MQ=255) gAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTAc < 1:1523595/71‑1 (MQ=255) gAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTAc > 1:2057428/1‑71 (MQ=255) aaCATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTAc > 1:4577611/1‑70 (MQ=255) aCATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGaa > 1:2556841/1‑45 (MQ=255) aCATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGaa > 1:2735683/1‑45 (MQ=255) aCATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGaa > 1:2467472/1‑45 (MQ=255) aCATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGaa > 1:2204159/1‑45 (MQ=255) aCATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACg < 1:1101927/70‑1 (MQ=255) cATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATa < 1:4115012/49‑1 (MQ=255) cATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATa < 1:4088829/49‑1 (MQ=255) aTCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCTTCGAGAATGATa < 1:3324702/48‑1 (MQ=255) ttCATCTGTTCGATGGTGGTGTTCTGGCTCTCTTCGAGAATGATAAACGCGTCGTTCAGCGTAc > 1:1895402/1‑64 (MQ=255) ttCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATaa > 1:3380947/1‑46 (MQ=255) ttCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATaa > 1:2655258/1‑46 (MQ=255) ttCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTa > 1:2112583/1‑63 (MQ=255) ttCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTAc > 1:2840705/1‑64 (MQ=255) ttCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTAc > 1:3156393/1‑64 (MQ=255) cATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCAc < 1:531802/68‑1 (MQ=255) aTCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCa < 1:3825442/70‑1 (MQ=255) tCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCata > 1:1646717/1‑71 (MQ=255) tCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCata > 1:3198458/1‑71 (MQ=255) tGTTCGATGGTGGTGTTCTGGCTCTCATCGAGGATGATAAACGCGTCGTTCAGCGTACGACCACGCata > 1:1186726/1‑69 (MQ=255) gTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCata > 1:302678/1‑68 (MQ=255) ttCGATGGTTGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCata < 1:165374/67‑1 (MQ=255) ttCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACg < 1:3809886/57‑1 (MQ=255) ttCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCata < 1:4196811/67‑1 (MQ=255) ttCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCata < 1:2711926/67‑1 (MQ=255) ttCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCata < 1:2727805/67‑1 (MQ=255) ttCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCata < 1:4695784/67‑1 (MQ=255) atCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAACCGCGTCGTTCAGCGTACGACCACGCata < 1:3172875/66‑1 (MQ=255) cGATGGTGGTGTTCTGGCTCTCATCGAGAATGATaaa > 1:2341350/1‑37 (MQ=255) cGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGCCCACGCATATAGGc > 1:588577/1‑70 (MQ=255) cGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGc > 1:1185104/1‑70 (MQ=255) cGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGc > 1:3962830/1‑70 (MQ=255) gATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGcc < 1:2045746/70‑1 (MQ=255) gATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGcc < 1:1113756/70‑1 (MQ=255) aTGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGcc < 1:4052627/69‑1 (MQ=255) tggtggTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGc < 1:4197899/71‑1 (MQ=255) tggtggTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGc < 1:4750944/71‑1 (MQ=255) tggtggTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGc < 1:1696876/71‑1 (MQ=255) tggtggTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGc > 1:1865383/1‑71 (MQ=255) tggtggTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGc < 1:2613602/71‑1 (MQ=255) tggtggTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGc < 1:3428267/71‑1 (MQ=255) tggtggTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGc < 1:2253320/71‑1 (MQ=255) tggtggTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGc < 1:2255534/71‑1 (MQ=255) cggtcgtGTTCTGGGCTTCGTCCAGGATCACGAACGCGTGGTTCAGGGTGCGCCCGCGCATATAGGCCAGc < 1:474649/70‑1 (MQ=255) cggtcgtGTTCTGGGCTTCGTCCAGGATCACGAACGCGTGGTTCAGGGTGCGCCCGCGCATATAGGCCAGc < 1:654269/70‑1 (MQ=255) gtggtgTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCg < 1:2001596/70‑1 (MQ=255) tggtgTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGt < 1:840300/71‑1 (MQ=255) tggtgTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGt < 1:2768528/71‑1 (MQ=255) ggtgTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGt > 1:3322729/1‑70 (MQ=255) ggtgTTCTGGCTCTAATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGt > 1:557101/1‑70 (MQ=255) gtgtTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGTGc > 1:849650/1‑71 (MQ=255) gtgtGCTGGCTCTCATCGAGAATGATAAACGCGGCGTTCAGCGTACGACCACGCATATAGGCCAGCGGTGc < 1:3044325/71‑1 (MQ=255) cTGGCTCTCATCGAGAATGATAAACGCGTCGTTCa > 1:3556052/1‑35 (MQ=255) cTGGCTCTCATCGAGAATGATAAACGCGTCGTTCa > 1:3487815/1‑35 (MQ=255) cTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCata > 1:4221569/1‑52 (MQ=255) cTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCata > 1:206683/1‑52 (MQ=255) cTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCata > 1:842776/1‑52 (MQ=255) cTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGTGCGACtt > 1:760054/1‑71 (MQ=255) | GATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGTGCGACTTCAA > minE/415879‑416018 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |