| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 415,960 | A→G | 6.0% | I253I (ATT→ATC) | ybeZ ← | hypothetical protein with nucleoside triphosphate hydrolase domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 415,960 | 0 | A | G | 6.0% | 301.9 / 8.2 | 100 | I253I (ATT→ATC) | ybeZ | hypothetical protein with nucleoside triphosphate hydrolase domain |
| Reads supporting (aligned to +/- strand): ref base A (45/49); new base G (4/2); total (49/51) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.32e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.22e-01 | |||||||||||
Only 100 of 111 total aligned reads displayed.
TGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGTGCGACTTCAATAACGTT > minE/415891‑416025 | tGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCgaga < 1:202153/70‑1 (MQ=255)tGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGaa > 1:1530854/1‑71 (MQ=255) tAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGAt > 1:4357761/1‑70 (MQ=255) aaaaCCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATa > 1:2623437/1‑70 (MQ=255) ccGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAAcgc > 1:2976042/1‑71 (MQ=255) gATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGcgt < 1:2846761/71‑1 (MQ=255) cGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGcgtcgt < 1:4644514/70‑1 (MQ=255) ggTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCa < 1:179884/71‑1 (MQ=255) agtaaCATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGt < 1:2819391/68‑1 (MQ=255) aGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGt < 1:3031544/71‑1 (MQ=255) aGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGt < 1:3891284/71‑1 (MQ=255) aGGAACATCTTCATCTGCTCGACGGTCGTGTTCTGGGCTTCGTCCAGGATCACGAACGCGTGGTTc > 1:3525706/1‑66 (MQ=255) aGGAACATCTTCATCTGCTCGACGGTCGTGTTCTGGGCTTCGTCCAGGATCACGAACGCGTGGTTCAgg > 1:1431861/1‑68 (MQ=255) ggAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGt < 1:370660/70‑1 (MQ=255) gAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTa > 1:2739599/1‑70 (MQ=255) gAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTa > 1:3005192/1‑70 (MQ=255) gAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTa > 1:4744366/1‑70 (MQ=255) gAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTAc > 1:2057428/1‑71 (MQ=255) gAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTAc > 1:3465459/1‑71 (MQ=255) aaCATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTAc > 1:4577611/1‑70 (MQ=255) aaCATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACg < 1:3546143/71‑1 (MQ=255) aCATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGaa > 1:2467472/1‑45 (MQ=255) aCATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGaa > 1:2556841/1‑45 (MQ=255) aCATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGaa > 1:2735683/1‑45 (MQ=255) aCATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGaa > 1:2204159/1‑45 (MQ=255) aCATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACg < 1:1101927/70‑1 (MQ=255) cATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATa < 1:4088829/49‑1 (MQ=255) cATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATa < 1:4115012/49‑1 (MQ=255) aTCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCTTCGAGAATGATa < 1:3324702/48‑1 (MQ=255) ttCATCTGTTCGATGGTGGTGTTCTGGCTCTCTTCGAGAATGATAAACGCGTCGTTCAGCGTAc > 1:1895402/1‑64 (MQ=255) ttCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATaa > 1:3380947/1‑46 (MQ=255) ttCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATaa > 1:2655258/1‑46 (MQ=255) ttCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTa > 1:2112583/1‑63 (MQ=255) ttCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTAc > 1:3156393/1‑64 (MQ=255) ttCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTAc > 1:2840705/1‑64 (MQ=255) cATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCAc < 1:531802/68‑1 (MQ=255) aTCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCa < 1:3825442/70‑1 (MQ=255) tCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCata > 1:1646717/1‑71 (MQ=255) tGTTCGATGGTGGTGTTCTGGCTCTCATCGAGGATGATAAACGCGTCGTTCAGCGTACGACCACGCata > 1:1186726/1‑69 (MQ=255) gTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCata > 1:302678/1‑68 (MQ=255) ttCGATGGTTGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCata < 1:165374/67‑1 (MQ=255) ttCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACg < 1:3809886/57‑1 (MQ=255) ttCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCata < 1:2727805/67‑1 (MQ=255) ttCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCata < 1:4196811/67‑1 (MQ=255) ttCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCata < 1:4695784/67‑1 (MQ=255) atCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAACCGCGTCGTTCAGCGTACGACCACGCata < 1:3172875/66‑1 (MQ=255) cGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGCCCACGCATATAGGc > 1:588577/1‑70 (MQ=255) cGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGc > 1:3962830/1‑70 (MQ=255) cGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGc > 1:1185104/1‑70 (MQ=255) gATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGcc < 1:1113756/70‑1 (MQ=255) gATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGcc < 1:2045746/70‑1 (MQ=255) aTGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGcc < 1:4052627/69‑1 (MQ=255) tggtggTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGc < 1:1696876/71‑1 (MQ=255) tggtggTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGc < 1:3428267/71‑1 (MQ=255) tggtggTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGc < 1:2253320/71‑1 (MQ=255) tggtggTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGc < 1:2613602/71‑1 (MQ=255) tggtggTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGc < 1:4197899/71‑1 (MQ=255) tggtggTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGc < 1:2255534/71‑1 (MQ=255) tggtggTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGc < 1:4750944/71‑1 (MQ=255) tggtggTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGc > 1:1865383/1‑71 (MQ=255) cggtcgtGTTCTGGGCTTCGTCCAGGATCACGAACGCGTGGTTCAGGGTGCGCCCGCGCATATAGGCCAGc < 1:654269/70‑1 (MQ=255) cggtcgtGTTCTGGGCTTCGTCCAGGATCACGAACGCGTGGTTCAGGGTGCGCCCGCGCATATAGGCCAGc < 1:474649/70‑1 (MQ=255) tggtgTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGt < 1:840300/71‑1 (MQ=255) tggtgTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGt < 1:2768528/71‑1 (MQ=255) gtgtgtTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCAc < 1:1532803/52‑1 (MQ=255) ggtgTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGt > 1:3322729/1‑70 (MQ=255) ggtgTTCTGGCTCTAATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGt > 1:557101/1‑70 (MQ=255) gtgtTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGTGc > 1:849650/1‑71 (MQ=255) gtgtGCTGGCTCTCATCGAGAATGATAAACGCGGCGTTCAGCGTACGACCACGCATATAGGCCAGCGGTGc < 1:3044325/71‑1 (MQ=255) gtTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAg > 1:4430137/1‑58 (MQ=255) cTGGCTCTCATCGAGAATGATAAACGCGTCGTTCa > 1:3556052/1‑35 (MQ=255) cTGGCTCTCATCGAGAATGATAAACGCGTCGTTCa > 1:3487815/1‑35 (MQ=255) cTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCata > 1:842776/1‑52 (MQ=255) cTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCata > 1:206683/1‑52 (MQ=255) cTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCata > 1:4221569/1‑52 (MQ=255) cTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGTGCGACtt > 1:760054/1‑71 (MQ=255) ctctCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCat > 1:2979153/1‑47 (MQ=255) ctctCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGTGCGACTTCaa > 1:2452422/1‑70 (MQ=255) tctcATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGTGCGACTTCAAt > 1:2887041/1‑70 (MQ=255) tcATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGTGCGACTTCAATAAc > 1:4115116/1‑71 (MQ=255) tcATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGTGCGACTTCAATAAc > 1:320400/1‑71 (MQ=255) cATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCAtata > 1:2515331/1‑46 (MQ=255) cATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCAtata > 1:3731579/1‑46 (MQ=255) aTCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCata > 1:2784660/1‑43 (MQ=255) aTCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGTGCGACTTCAATaa < 1:2098809/68‑1 (MQ=255) aTCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGTGCGACTTCAATaa < 1:3880971/68‑1 (MQ=255) aTCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGTGCGACTTCAATAAc < 1:2729676/69‑1 (MQ=255) aTCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGTGCGACTTCAATAAc < 1:1161111/69‑1 (MQ=255) aTCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGTGCGACTTCAATAACg < 1:1342408/70‑1 (MQ=255) aTCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGTGCGACTTCAATAACGt > 1:560368/1‑71 (MQ=255) aTCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGTGCGACTTCAATAACGt > 1:3830188/1‑71 (MQ=255) aTCGAGAATGATAAACGCGTCGTTCAGCGTACGACAACGCATATAGGCCAGCGGTGCGACTTCAATAACGt > 1:3218378/1‑71 (MQ=255) tCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCa < 1:1090200/40‑1 (MQ=255) tCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGTGCGACt < 1:638351/60‑1 (MQ=255) tCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGTGCGACt < 1:1515911/60‑1 (MQ=255) tCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGTGCGACTTCAATAACGtt < 1:1814320/71‑1 (MQ=255) gagaATGATAAACGCGTCGTTCAGCGTACGACCACGCa < 1:2504673/38‑1 (MQ=255) gagaATGATAAACGCGTCGTTCAGCGTACGACCACGCa < 1:1378749/38‑1 (MQ=255) agaATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGTg < 1:2459290/52‑1 (MQ=255) agaATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGTg < 1:4286083/52‑1 (MQ=255) | TGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGTGCGACTTCAATAACGTT > minE/415891‑416025 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |