| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 539,433 | T→C | 7.5% | V132V (GTA→GTG) | dps ← | Fe‑binding and storage protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 539,433 | 0 | T | C | 7.5% | 276.9 / 11.1 | 93 | V132V (GTA→GTG) | dps | Fe‑binding and storage protein |
| Reads supporting (aligned to +/- strand): ref base T (45/41); new base C (2/5); total (47/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.67e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.68e-01 | |||||||||||
CAGGTCGCGAGACGCGGCGGTCAGGATATCTGCGGTGTCGTCATCTTTCGCTTCGCCAATCGCTTTGCGTACGTCATTAGCGACGATTGCGTAACGGTCAGCCAGTTCTTTCAGGTGATCCTGAACGTTGTGGATGT > minE/539364‑539500 | cAGGTCGCGAGACGCGGCGGTCAGGATATCTGCGGTGTCGTCATCTTTCGCTTCGCCAATCGCTTTGCGt < 1:3318879/70‑1 (MQ=255)cAGGTCGCGAGACGCGGCGGTCAGGATATCTGCGGTGTCGTCATCTTTCGCTTCGCCAATCGCTTTGCGt < 1:1756777/70‑1 (MQ=255) aGGTCGCGAGACGCGGCGGTCAGGATATCTGCGGTGTCGTCATCTTTCGCTTCGCCAATCGCTTTGCGTAc < 1:1620344/71‑1 (MQ=255) gTCGCGAGACGCGGCGGTCAGGATATCTGCGGTGTCGTCATCTTTCGCTTCGCCAATCGCTTTGCGTACGt < 1:2555747/71‑1 (MQ=255) gTCGCGAGACGCGGCGGTCAGGATATCTGCGGTGTCGTCATCTTTCGCTTCGCCAATCGCTTTGCGTACGt > 1:2034545/1‑71 (MQ=255) gTCGCGAGACGCGGCGGTCAGGATATCTGCGGTGTCGTCATCTTTCGCTTCGCCAATCGCTTTGCGTACGt < 1:2577041/71‑1 (MQ=255) cgcgAGACGCGGCGGTCAGGATATCTGCGGTGTCGTCATCTTTCGCTTCGCCAATCGCTTTGCGTACGTCa > 1:63409/1‑71 (MQ=255) cgcgAGACGCGGCGGTCAGGATATCTGCGGTGTCGTCATCTTTCGCTTCGCCAATCGCTTTGCGTACGTCa > 1:2101181/1‑71 (MQ=255) gcgAGACGCGGCGGTCAGGATATCTGCGGTGTCGTCATCTTTCGCTTCGCCAATCGCTTTGCGTACGTCa < 1:4476042/70‑1 (MQ=255) gcgAGACGCGGCGGTCAGGATATCTGCGGTGTCGTCATCTTTCGCTTCGCCAATCGCTTTGCGTACGTCa < 1:2196974/70‑1 (MQ=255) gcgAGACGCGGCGGTCAGGATATCTGCGGTGTCGTCATCTTTCGCTTCGCCAATCGCTTTGCGTACGTCa < 1:2044145/70‑1 (MQ=255) cgAGACGCGGCGGTCAGGATATCTGCGGTGTCGTCATCTTTCGCTTCGCCAATCGCTTTGCGTACGTCAtt < 1:4674261/71‑1 (MQ=255) cgAGACGCGGCGGTCAGGATATCTGCGGTGTCGTCATCTTTCGCTTCGCCAATCGCTTTGCGTACGTCAtt < 1:3606796/71‑1 (MQ=255) gagaCGCGGCGGTCAGGATATCTGCGGTGTCGTCATCTTTCGCTTCGCCAATCGCTTTGCGTACGTCAt > 1:2082601/1‑69 (MQ=255) gagaCGCGGCGGTCAGGATATCTGCGGTGTCGTCATCTTTCGCTTCGCCAATCGCTTTGCGCACGTCAtt < 1:373994/70‑1 (MQ=255) gagaCGCGGCGGTCAGGATATCTGCGGTGTCGTCATCTTTCGCTTCGCCAATCGCTTTGCGCACGTCAtt < 1:1691773/70‑1 (MQ=255) gagaCGCGGCGGTCAGGATATCTGCGGTGTCGTCATCTTTCGCTTCGCCAATCGCTTTGCGCACGTCAtt < 1:1218232/70‑1 (MQ=255) gagaCGCGGCGGTCAGGATATCTGCGGTGTCGTCATCTTTCGCTTCGCCAATCGCTTTGCGCACGTCAtt < 1:4148569/70‑1 (MQ=255) gagaCGCGGCGGTCAGGATATCTGCGGTGTCGTCATCTTTCGCTTCGCCAATCGCTTTGCGCACGTCAtt < 1:551324/70‑1 (MQ=255) gaCGCGGCGGTCAGGATATCTGCGGTGTCGTCATCTTTCGCTTCGCCAATCGCTTTGCGTACGTCATTAGc > 1:1655016/1‑71 (MQ=255) gaCGCGGCGGTCAGGATATCTGCGGTGTCGTCATCTTTCGCTTCGCCAATCGCTTTGCGTACGTCATTAGc > 1:3175187/1‑71 (MQ=255) gaCGCGGCGGTCAGGATATCTGCGGTGTCGTCATCTTTCGCTTCGCCAATCGCTTTGCGCACGTCATTAg > 1:2520313/1‑70 (MQ=255) gaCGCGGCGGTCAGGATATCTGCGGTGTCGTCATCTTTCGCTTAGCCAATCGCTTTGCGCACGTCATTAg > 1:4373151/1‑70 (MQ=255) aCGCGGCGGTCAGGATATCTGCGGTGTCGTCATCTTTCGCTTCGCCAATCGCTTTGCGTACGTCATTAGc > 1:3532098/1‑70 (MQ=255) aCGCGGCGGTCAGGATATCTGCGGTGTCGTCATCTTTCGCTTCGCCAATCGCTTTGCGTACGTCATTAGc > 1:270191/1‑70 (MQ=255) gcggcgGTCAGGATATCTGCGGTGTCGTCATCTTTCGCTTCGCCAATCGCTTTGCGTACGTCATTAGcgac > 1:3613412/1‑71 (MQ=255) cggcggTCAGGATATCTGCGGTGTCGTCATCTTTCGCTTCGCCAATCGCTTTGCGTACGTCATTAGcgacg < 1:699837/71‑1 (MQ=255) cggcggTCAGGATATCTGCGGTGTCGTCATCTTTCGCTTCGCCAATCGCTTTGCGTACGTCATTAGcgacg < 1:223168/71‑1 (MQ=255) gTCAGGATATCTGCGGTGTCGTCATCTTTCGCTTCGCCAATCGCTTTGCGTACGTCATTAGcgacg < 1:1012496/66‑1 (MQ=255) tCAGGATATCTGCGGTGTCGTCATCTTTCGCTTCGCCAATCGCTTTGCGTACGTCATTAGCGACGATTGCg > 1:3373484/1‑71 (MQ=255) cAGGATATCTGCGGTGTCGTCATCTTTCGCTTCGCCAATCGCTTTGCGTACGTCATTa > 1:1109928/1‑58 (MQ=255) ggATATCTGCGGTGTCGTCATCTTTCGCTTCGCCAATCGCTTTGCGTACGTCATTAGCGACGATTGCGTaa < 1:625700/71‑1 (MQ=255) gATATCTGCGGTGTCGTCATCTTTCGCTTCGCCAATCGCTTTGCGTACGTCATTAGCGACGATTGCGTAAc > 1:3421991/1‑71 (MQ=255) gATATCTGCGGTGTCGTCATCTTTCGCTTCGCCAATCGCTTTGCGTACGTCATTAGCGACGATTGCGTAAc > 1:2541540/1‑71 (MQ=255) tatCTGCGGTGTCGTCATCTTTCGCTTCGCCAATCGCTTTGCGTACGTCATTAGCGACGATTGCGTAACgg > 1:1012183/1‑71 (MQ=255) tGCGGTGTCGTCATCTTTCGCTTCGCCAATCGCTTTGCGTACGTCATTAGcgac > 1:3114703/1‑54 (MQ=255) tGCGGTGTCGTCATCTTTCGCTTCGCCAATCGCTTTGCGTACGTCATTAGcgac > 1:959799/1‑54 (MQ=255) cGGTGTCGTCATCTTTCGCTTCGCCAATCGCTTTGCGTACGTCATTAGCGACGATTGCGTAACGGTCAGc > 1:1853102/1‑70 (MQ=255) tcgtcATCTTTCGCTTCGCCAATCGCTTTGCGTACGTCATTAGCCCCGATTGCGt > 1:3799222/1‑55 (MQ=255) gtcATCTTTCGCTTCGCCAATCGCTTTGCGTACGTCATTAGCGACGATTGCGTAACGGTCAGCCAGTTc < 1:1221106/69‑1 (MQ=255) gtcATCTTTCGCTTCGCCAATCGCTTTGCGTACGTCATTAGCGACGATTGCGTAACGGTCAGCCAGTTc < 1:3872477/69‑1 (MQ=255) gtcATCTTTCGCTTCGCCAATCGCTTTGCGTACGTCATTAGCGACGATTGCGTAACGGTCAGCCAGTTc < 1:269894/69‑1 (MQ=255) gtcATCTTTCGCTTCGCCAATCGCTTTGCGTACGTCATTAGCGACGATTGCGTAACGGTCAGCCAGTTCtt > 1:235059/1‑71 (MQ=255) gtcATCTTTCGCTTCGCCAATCGCTTTGCGTACGTCATTAGCGACGATTGCGTAACGGTCAGCCAGTTCt < 1:4295028/70‑1 (MQ=255) gtcATCTTTCGCTGCGCCAATCGCTTTGCGTACGTCATTAg < 1:4005168/41‑1 (MQ=255) tcATCTTTCGCTTCGCCAATCGCTTTGCGTACGTCATTAGCGACGATTGCGTAACGGTCAGCCAGTTCttt < 1:1066643/71‑1 (MQ=255) tcATCTTTCGCTTCGCCAATCGCTTTGCGTACGTCATTAGCGACGATTGCGTAACGGTCAGCCAGTTCttt < 1:1507555/71‑1 (MQ=255) tcATCTTTCGCTTCGCCAATCGCTTTGCGTACGTCATTAGCGACGATTGCGTAACGGTCAGCCAGTTCtgt < 1:3211853/71‑3 (MQ=255) aTCTTTCGCTTCGCCAATCGCTTTGCGTACGTCATTAGCGACGATTGCGTAACGGTCAGCCAGTTCttt > 1:854449/1‑69 (MQ=255) aTCTTTCGCTTCGCCAATCGCTTTGCGTACGTCATTAGCGACGATTGCGTAACGGTCAGCCAGTTCttt > 1:4573221/1‑69 (MQ=255) aTCTTTCGCTTCGCCAATCGCTTTGCGTACGTCATTAGCGACGATTGCGTAACGGTCAGCCAGTTCt > 1:2227540/1‑67 (MQ=255) tCTTTCGCTTCGCCAATCGCTTTGCGTACGTCATTAGCGACGATTGCGTAACGGTCAGCCAGt < 1:2344504/63‑1 (MQ=255) tCTTTCGCTTCGCCAATCGCTTTGCGTACGTCATTAGCGACGATTGCGTAACGGTCAGCCAGt < 1:2151191/63‑1 (MQ=255) tCTTTCGCTTCGCCAATCGCTTTGCGTACGTCATTAGCGACGATTGCGTAACGGTCAGCCAGt < 1:728736/63‑1 (MQ=255) tCTTTCGCTTCGCCAATCGCTTTGCGTACGTCATTAGCGACGATTGCGTAACGGTCAGCCAGt < 1:1701597/63‑1 (MQ=255) tCTTTCGCTTCGCCAATCGCTTTGCGTACGTCATTAGCGACGATTGCGTAACGGTCAGCCAGt < 1:3955287/63‑1 (MQ=255) tCTTTCGCTTCGCCAATCGCTTTGCGTACGTCATTAGCGACGATTGCGTAACGGTCAGCCAGt < 1:2209235/63‑1 (MQ=255) tCTTTCGCTTCGCCAATCGATTTGCGTACGTCATTAGCGACGATTGCGTAACGGTCAGCCAGt < 1:151320/63‑1 (MQ=255) cTTTCGCTTCGCCAATCGCTTTGCGTACGTCATTAGcgac > 1:4467551/1‑40 (MQ=255) cTTTCGCTTCGCCAATCGCTTTGCGTACGTCATTAGcgac > 1:1142341/1‑40 (MQ=255) cTTTCGCTTCGCCAATCGCTTTGCGTACGTCATTAGCGACGATTGCGTAACGGTc > 1:3736170/1‑55 (MQ=255) cTTTCGCTTCGCCAATCGCTTTGCGTACGTCATTAGCGACGATTGCGTAACGGTc > 1:39396/1‑55 (MQ=255) cTTTCGCTTCGCCAATCGCTTTGCGTACGTCATTAGCGACGATTGCGTAACGGTc > 1:2461658/1‑55 (MQ=255) cTTTCGCTTCGCCAATCGCTTTGCGTACGTCATTAGCGACGATTGCGTAACGGTc > 1:3437394/1‑55 (MQ=255) cTTTCGCTTCGCCAATCGCTTTGCGTACGTCATTAGCGACGATTGCGTAACGGTc > 1:743323/1‑55 (MQ=255) tttCGCTTCGCCAATCGCTTTGCGTACGTCATTAGCGACGATTGCGTAACGGTCAGCCAGTTCTTTCAGGt > 1:4187738/1‑71 (MQ=255) tttCGCTTCGCCAATCGCTTTGCGTACGTCATTAGCGACGATTGCGTAACGGTCAGCCAGTTCTTTCAGGt > 1:227971/1‑71 (MQ=255) ttcgcttcgcCAATCGCTTTGCGTACGTCATTAGCGACGAt > 1:2681437/1‑41 (MQ=255) ttcgcttcgcCAATCGCTTTGCGTACGTCATTAGCGACGAt > 1:4552551/1‑41 (MQ=255) cgcttcgcCAATCGCTTTGCGTACGTCATTAGCGACGATTGCGTAACGGTCAGCCAGTTCTTTCAGGTGa < 1:4119343/70‑1 (MQ=255) gcttcgcCAATCGCTTTGCGTACGTCATTAGCGACGATTGCGTAACGGTCAGCCAGTTCTTTCAGGTGAt < 1:2063998/70‑1 (MQ=255) ttcgcCAATCGCTTTGCGTACGTCATTAGCGACGATTGCGTAACGGTCAGCCAGTTCTTTCAGGTGATCCt > 1:1221642/1‑71 (MQ=255) tcgcCAATCGCTTTGCGTACGTCATTAGCGACGATTGCGTAACGGTCAGCCAGTTCTTTCAGGTGATCCt > 1:1483666/1‑70 (MQ=255) cgcCAATCGCTTTGCGTACGTCATTAGCGACGATTGCGTAACGGTCAGCCAGTTCTTTCAGGTGATCCTg > 1:3696813/1‑70 (MQ=255) cgcCAATCGCTTTGCGTACGTCATTAGCGACGATTGCGTAACGGTCAGCCAGTTCTTTCAGGTGATCCTg > 1:1257251/1‑70 (MQ=255) cgcCAATCGCTTTGCGTACGTCATTAGCGACGATTGCGTAACGGTCAGCCAGTTCTTTCAGGTGATCCTg > 1:2548571/1‑70 (MQ=255) cgcCAATCGCTTTGCGTACGTCATTAGCGACGATTGCGTAACGGTCAGCCAGTTCTTTCAGGTGATCCTg > 1:3387320/1‑70 (MQ=255) aaTCGCTTTGCGTACGTCATTAGCGACGATTGCGTAACGGTCAGCCAGTTCTTTCAGGTGATCCTGAACGt < 1:1518852/71‑1 (MQ=255) aaTCGCTTTGCGTACGTCATTAGCGACGATTGCGTAACGGTCAGCCACTTCTTTCAGGTGATCCTGAACGt < 1:2898700/71‑1 (MQ=255) aTCGCTTTGCGTACGTCATTAGCGACGATTGCGTAACGGTCAGCCAGTTCTTTCAGGTGATCCTGAACGtt > 1:185442/1‑71 (MQ=255) aTCGCTTTGCGTACGTCATTAGCGACGATTGCGTAACGGTCAGCCAGTTCTTTCAGGTGATCCTGAACGt < 1:1998694/70‑1 (MQ=255) aTCGCTTTGCGTACGTCATTAGCGACGATTGCGTAACGGTCAGCCAGTTCTTTCAGGTGATCCTGAACGt > 1:4558850/1‑70 (MQ=255) tCGCTTTGCGTACGTCATTAGCGACGATTGCGTAACGGTCAGCCAGTTCTTTCAGGTGATCCTGAACGTtg < 1:2552308/71‑1 (MQ=255) tCGCTTTGCGTACGTCATTAGCGACGATTGCGTAACGGTCAGCCAGTTCTTTCAGGTGATCCTGAACGTtg < 1:1529562/71‑1 (MQ=255) tCGCTTTGCGTACGTCATTAGCGACGATTGCGTAACGGTCAGCCAGTTCTTTCAGGTGATCCTGAACGTtg < 1:3723952/71‑1 (MQ=255) tCGCTTTGCGTACGTCATTAGCGACGATTGCGTAACGGTCAGCCAGTTCTTTCAGGTGATCCTGAACGTtg < 1:3158847/71‑1 (MQ=255) gCTTTGCGTACGTCATTAGCGACGATTGCGTAACGGTCAGCCAGTTCTTTCAGGTGATCCTGAACGTtgtg > 1:2328301/1‑71 (MQ=255) gCTTTGCGTACGTCATTAGCGACGATTGCGTAACGGTCAGCCAGTTCTTTCAGGTGATCCTGAACGTtgtg > 1:4365133/1‑71 (MQ=255) gCTTTGCGTACGTCATTAGCGACGATTGCGTAACGGTCAGCCAGTTCTTTCAGGTGATCCTGAACGTtgtg > 1:1945514/1‑71 (MQ=255) gCTTTGCGTACGTCATTAGCGACGATTGCGTAACGGTCAGCCAGTTCTTTCAGGTGATCCTGAACGTtgtg > 1:219107/1‑71 (MQ=255) cTTTGCGTACGTCATTAGCGACGATTGCGTAACGGTCAGCCAGTTCTTTCAGGTGATCCTGAACGTTGTgg < 1:1677097/71‑1 (MQ=255) cTTTGCGTACGTCATTAGCGACGATTGCGTAACGGTCAGCCAGTTCTTTCAGGTGATCCTGAACGTTGTgg < 1:3504009/71‑1 (MQ=255) cTTTGCGTACGTCATTAGCGACGATTGCGTAACGGTCAGCCAGTTCTTTCAGGTGATCCTGAACGTTGTgg < 1:3442409/71‑1 (MQ=255) cTTTGCGTACGTCATTAGCGACGATTGCGTAACGGTCAGCCAGTTCTTTCAGGTGATCCTGAACGTTGTgg < 1:3019641/71‑1 (MQ=255) cGTACGTCATTAGCGACGATTGCGTAACGGTCAGCCAGTTCTTTCAGGTGATCCTGAACGTTGTGGATGt < 1:2661567/70‑1 (MQ=255) | CAGGTCGCGAGACGCGGCGGTCAGGATATCTGCGGTGTCGTCATCTTTCGCTTCGCCAATCGCTTTGCGTACGTCATTAGCGACGATTGCGTAACGGTCAGCCAGTTCTTTCAGGTGATCCTGAACGTTGTGGATGT > minE/539364‑539500 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |