| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 778,653 | T→G | 5.1% | intergenic (‑359/‑161) | minC ← / → ycgJ | cell division inhibitor/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 778,653 | 0 | T | G | 5.1% | 226.5 / 6.3 | 79 | intergenic (‑359/‑161) | minC/ycgJ | cell division inhibitor/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (47/28); new base G (2/2); total (49/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.32e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01 | |||||||||||
TTTTTTCACCACGCCAGGCTTTCTTAACTTTCCATTTTTTGCGGCATCATTAAAGAACAGATACCCGTTATCTTAATATCCGCCACAGAATTGCTCTATCATAGAAACCGCAGAAGACGTCTGATACTTCTGTCAGGT > minE/778586‑778723 | ttttttCACCACGCCAGGCTTTCTTAACTTTCCATTTTTTGCGGCATCATTAAAGAACAGATACCCGTTa < 1:525095/70‑1 (MQ=255)ttttttCACCACGCCAGGCTTTCTTAACTTTCCATTTTTTGCGGCATCATTAAAGAACAGATACCCGTTAt > 1:4567335/1‑71 (MQ=255) ttttCACCACGCCAGGCTTTCTTAACTTTCCATTTTTTGCGGCATCATTAAAGAACAGATACCCGTTAt > 1:2573946/1‑69 (MQ=255) ttttCACCACGCCAGGCTTTCTTAACTTTCCATTTTTTGCGGCATCATTAAAGAACAGATACCCGTTAt > 1:112673/1‑69 (MQ=255) ttttCACCACGCCAGGCTTTCTTAACTTTCCATTTTTTGCGGCATCATTAAAGAACAGATACCCGTTAt > 1:4715025/1‑69 (MQ=255) ttttCACCACGCCAGGCTTTCTTAACTTTCCATTTTTTGCGGCATCATTAAAGAACAGATACCCGTTAt > 1:3674252/1‑69 (MQ=255) ttttCACCACGCCAGGCTTTCTTAACTTTCCATTTTTTGCGGCATCATTAAAGAACAGATACCCGTTATc < 1:337859/70‑1 (MQ=255) ttttCACCACGCCAGGCTTTCTTAACTTTCCATTTTTTGCGGCATCATTAAAGAACAGATACCCGTTATCt > 1:4216857/1‑71 (MQ=255) ttttCACCACGCCAGGCTTTCTTAACTTTCCATTTTTTGCGGCATCATTAAAGAACAGATAACGGGTATCt > 1:2217585/1‑71 (MQ=255) ttttCACCACGCCAGGCTTTCTTAACTTTCCATTTTTTGCGGCATCATTAAAGAACAGATAACGGGTATCt > 1:3142079/1‑71 (MQ=255) ttCACCACGCCAGGCTTTCTTAACTTTCCATTTTTTGCGGCATCATTAAAGAACAGATACCCGTTATCtt < 1:1136818/70‑1 (MQ=255) ttCACCACGCCAGGCTTTCTTAACTTTCCATTTTTTGCGGCATCATTAAAGAACAGATAACGGGTATCtg < 1:1733582/70‑2 (MQ=255) accacGCCAGGCTTTCTTAACTTTCCATTTTTTGCGGCATCATTAAAGAACAGATAACGGGTATCtg < 1:3166201/67‑2 (MQ=255) gCCAGGCTTTCTTAACTTTCCATTTTTTGCGGCATCATTAAAGAACAGATACCCGTTATCTTAATATCCGc > 1:3969170/1‑71 (MQ=255) ccAGGCTTTCTTAACTTTCCATTTTTTGCGGCATCATTAAAGAACAGATACCCGTTATCTTAATATCCGc > 1:1804013/1‑70 (MQ=255) aGGCTTTCTTAACTTTCCATTTTTTGCGGCATCATTAAAGAACAGATACCCGTTATCTTAATATCCGCca > 1:556056/1‑70 (MQ=255) ggCTTTCTTAACTTTCCATTTTTTGCGGCATCATTAAAGAACAGATACCCGTTATCTTAATATCCGCca > 1:3613147/1‑69 (MQ=255) ggCTTTCTTAACTTTCCATTTTTTGCGGCATCATTAAAGAACAGATACCCGTTATCTTAATATCCGCca > 1:4743759/1‑69 (MQ=255) aCTTTCCATTTTTTGCGGCATCATTAAAGAACAGATACCCGTTATCTTAATATCCGCCACAGAATTGctc > 1:4535597/1‑70 (MQ=255) aCTTTCCATTTTTTGCGGCATCATTAAAGAACAGATACCCGTTATCTTAATATCCGCCACAGAATTGctc > 1:3291955/1‑70 (MQ=255) aCTTTCCATTTTTTGCGGCATCATTAAAGAACAGATACCCGTTATCTTAATATCCGCCACAGAATTGctc > 1:4013157/1‑70 (MQ=255) cTTTCCATTTTTTGCGGCATCATTAAAGAACAGATACCCGTTATCTTAATATCCGcc > 1:3678830/1‑57 (MQ=255) tttCCATTTTTTGCGGCATCATTAAAGAACAGATACCCGTTATCTTAATATCCGCCACAGa > 1:4691493/1‑61 (MQ=255) tttCCATTTTTTGCGGCATCATTAAAGAACAGATACCCGTTATCTTAATATCCGCCACAGa > 1:3934900/1‑61 (MQ=255) tttCCATTTTTTGCGGCATCATTAAAGAACAGATACCCGTTATCTTAATATCCGCCACAGa > 1:811176/1‑61 (MQ=255) tttCCATTTTTTGCGGCATCATTAAAGAACAGATACCCGTTATCTTAATATCCGCCACAGa > 1:1216073/1‑61 (MQ=255) tttCCATTTTTTGCGGCATCATTAAAGAACAGATACCCGTTATCTTAATATCCGCCACAGa > 1:1399211/1‑61 (MQ=255) tttCCATTTTTTGCGGCATCATTAAAGAACAGATACCCGTTATCTTAATATCCGCCACAGa > 1:518381/1‑61 (MQ=255) tttCCATTTTTTGCGGCATCATTAAAGAACAGATACCCGTTATCTTAATATCCGCCACAGa > 1:1872579/1‑61 (MQ=255) tCCATTTTTTGCGGCATCATTAAAGAACATATACCCGTTATCTTAATATCCGCCACAGAATTGCTCTATCa > 1:3825653/1‑71 (MQ=255) tCCATTTTTTGCGGCATCATTAAAGAACAGATACCCGTTATCTTAATATCCGCCACAGAATTGCTCTATCa > 1:2556654/1‑71 (MQ=255) tCCATTTTTTGCGGCATCATTAAAGAACAGATACCCGTTATCTTAATATCCGCCACAGAATTGCTCTATCa > 1:2973035/1‑71 (MQ=255) ccATTTTTTGCGGCATCATTAAAGAACAGATACCCGTTATCTTAATATCCGCCACAGAATTGCTCTATCa > 1:3716847/1‑70 (MQ=255) cATTTTTTGCGGCATCATTAAAGAACAGATACCCGTTATCTTAATATCCGCCACAGAATTGCTCTATCa > 1:630364/1‑69 (MQ=255) ttttttGCGGCATCATTAAAGAACAGATACCCGTTATCTTAATATCCGCCACAGAATTGCTCTATCATAGa < 1:3173538/71‑1 (MQ=255) tttttGCGGCATCATTAAAGAACAGATACCCGTTATCTTAat > 1:2572496/1‑42 (MQ=255) tttttGCGGCATCATTAAAGAACAGATACCCGTTATCTTAat > 1:4548897/1‑42 (MQ=255) ttttGCGGCATCATTAAAGAACAGATACCCGTTATCTTaa > 1:1819419/1‑40 (MQ=255) ttttGCGGCATCATTAAAGAACAGATACCCGTTATCTTaa > 1:570561/1‑40 (MQ=255) ttttGCGGCATCATTAAAGAACAGATACCCGTTATCTTaa > 1:2313587/1‑40 (MQ=255) ttttGCGGCATCATTAAAGAACAGATACCCGTTATCTTAATATCCTCCACAGAATTGCTCTATCATAGaa < 1:1780487/70‑1 (MQ=255) ttttGCGGCATCATTAAAGAACAGATACCCGTTATCTTAATATCCGCCACAGAATTGCTCTATCATAGaa < 1:491144/70‑1 (MQ=255) ttttGCGGCATCATTAAAGAACAGATACCCGTTATCTTAATATCCGCCACAGAATTGCTCTATCATAGaa < 1:1991353/70‑1 (MQ=255) tttGCGGCATCATTAAAGAACAGATACCCGTTATCTTAATATCCGCCACAGAATTGCTCTATCATAGaaa < 1:117948/70‑1 (MQ=255) catcatTAAAGAACAGATACCCGTTATCTTAATATCCGCCACAGAATTGCTCTATCATAGAAACCGCagaa < 1:3147323/71‑1 (MQ=255) catcatTAAAGAACAGATACCCGTTATCTTAATATCCGCCACAGAATTGCTCTATCATAGAAACCGCagaa < 1:3463048/71‑1 (MQ=255) catcatTAAAGAACAGATACCCGTTATCTTAATATCCGCCACAGAATTGCTCTATCATAGAAACCGCagaa < 1:3091298/71‑1 (MQ=255) atcatTAAAGAACAGATACCCGTTATCTTAATATCCGCCACAGAATTGCTCTATCATAGAAACCGCagaag < 1:97421/71‑1 (MQ=255) tcatTAAAGAACAGATACCCGTTATCTTAATATCCGCCACAGAATTGCTCTATCa < 1:1002966/55‑1 (MQ=255) tcatTAAAGAACAGATACCCGTTATCTTAATATCCGCCACAGAATTGCTCTATCa < 1:4226830/55‑1 (MQ=255) tcatTAAAGAACAGATACCCGTTATCTTAATATCCGCCACAGAATTGCTCTATCa < 1:138761/55‑1 (MQ=255) tcatTAAAGAACAGATACCCGTTATCTTAATATCCGCCACAGAATTGCTCTATCATAGAAACCGCagaaga > 1:168371/1‑71 (MQ=255) tcatTAAAGAACAGATACCCGTTATCTTAATATCCGCCACAGAATTGCTCTATCATAGAAACCGCagaaga > 1:4509603/1‑71 (MQ=255) tcatTAAAGAACAGATACCCGTTATCTTAATATCCGCCACAGAATTACTCTATCa < 1:4114202/55‑1 (MQ=255) atTAAAGAACAGATACCCGTTATCTTAATATCCGCCACAGAATTGCTCTATCATAGAAACCGCAGAAGAc < 1:1642819/70‑1 (MQ=255) ttAAAGAACAGATACCCGTTATCTTAATATCCGCCACAGAATTGCTCTATCATAGAAACCGCAGAAGACGt < 1:3252349/71‑1 (MQ=255) ttAAAGAACAGATACCCGTTATCTTAATATCCGCCACAGAATTGCTCTATCATAGAAACCGCAGAAGACGt < 1:4736901/71‑1 (MQ=255) tAAAGAACAGATACCCGTTATCTTAATATCCGCCACAGaa > 1:1085756/1‑40 (MQ=255) tAAAGAACAGATACCCGTTATCTTAATATCCGCCACAGaa > 1:736954/1‑40 (MQ=255) tAAAGAACAGATACCCGTTATCTTAATATCCGCCACAGaa > 1:2557916/1‑40 (MQ=255) tAAAGAACAGATACCCGTTATCTTAATATCCGCCACAGa > 1:4381275/1‑39 (MQ=255) cAGATACCCGTTATCTTAATATCCGCCACAGAATTGCTCTATCATAGAAACCGCAGAAGACGTCTGATACt > 1:4378500/1‑71 (MQ=255) cAGATACCCGTTATCTTAATATCCGCCACAGAATTGCTCTATCATAGAAACCGCAGAAGACGTCTGATACt < 1:4703686/71‑1 (MQ=255) aGATACCCGTTATCTTAATATCCGCCACAGAATTGCTCTATCATAGAAACCGCAGAAGACGTCTGATACtt > 1:4621857/1‑71 (MQ=255) aGATACCCGTTATCTTAATATCCGCCACAGAATTGCTCTATCATAGAAACCGCAGAAGACGTCTGATACtt < 1:4079734/71‑1 (MQ=255) aGATACCCGTTATCTTAATATCCGCCACAGAATTGCTCTATCATAGAAACCGCAGAAGACGTCTGATACtt > 1:4304873/1‑71 (MQ=255) aGATACCCGTTATCTTAATATCCGCCACAGAATTGCTCTATCATAGAAACCGCAGAAGACGTCTGATACt > 1:4246049/1‑70 (MQ=255) gATACCCGTTATCTTAATATCCGCCACAGAATTGCTCTATCATAGAAACCGCAGAAGACGTCTGATACTTc > 1:3931842/1‑71 (MQ=255) aTACCCGTTATCTTAATATCCGCCACAGAATTGCTCTATCATAGAAACCGCagaa < 1:2313939/55‑1 (MQ=255) aCCCGTTATCTTAATATCCGCCACAGAATTGCTCTATCATAGAAACCGCAGAAGACGTCTGATACTTCt > 1:4482667/1‑69 (MQ=255) cccGTTATCTTAATATCCGCCACAGAATTGCTCTATCATAGAAACCGCAGAAGACGTCTGATACTTCTGt > 1:1915873/1‑70 (MQ=255) cccGTTATCTTAATATCCGCCACAGAATTGCTCTATCATAGAAACCGCAGAAGACGTCTGATACTTCTGt > 1:1817861/1‑70 (MQ=255) ccGTTATCTTAATATCCGCCACAGAATTGCTCTATCATAGAAACCGCAGAAGACGt < 1:2900329/56‑1 (MQ=255) ccGTTATCTTAATATCCGCCACAGAATTGCTCTATCATAGAAACCGCAGAAGACGTCTGATACTTCTGTCa < 1:2403012/71‑1 (MQ=255) cGTTATCTTAATATCCGCCACAGAATTGCTCTATCATAGAAACCGCaga < 1:2898528/49‑1 (MQ=255) cGTTATCTTAATATCCGCCACAGAATTGCTCTATCATAGAAACCGCag < 1:1602690/48‑1 (MQ=255) cGTTATCTTAATATCCGCCACAGAATTGCTCTATCATAGAAACCGCAGAAGACGTCTGATACTTCTGTCAg < 1:362990/71‑1 (MQ=255) cGTTATCTTAATATCCGCCACAGAATTGCTCTATCATAGAAACCGCAGAAGACGTCTGATACTTCTGTCAg < 1:4749688/71‑1 (MQ=255) cGTTATCTTAATATCCGCCACAGAATTGCTCTATCATAGAAACCGCAGAAGACGTCTGATACTTCTGTCAg > 1:281326/1‑71 (MQ=255) ttATCTTAATATCCGCCACAGAATTGCTCTATCATAGAAACCGCAGAAGACGTCTGATACTTCTGTCAGGt > 1:4358980/1‑71 (MQ=255) ttATCTTAATATCCGCCACAGAATTGCTCTATCATAGAAACCGCAGAAGACGTCTGATACTTCTGTCAGGt > 1:897745/1‑71 (MQ=255) | TTTTTTCACCACGCCAGGCTTTCTTAACTTTCCATTTTTTGCGGCATCATTAAAGAACAGATACCCGTTATCTTAATATCCGCCACAGAATTGCTCTATCATAGAAACCGCAGAAGACGTCTGATACTTCTGTCAGGT > minE/778586‑778723 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |