| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 828,622 | T→C | 7.2% | S226G (AGT→GGT) | narX ← | sensory histidine kinase in two‑component regulatory system with NarL |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 828,622 | 0 | T | C | 7.2% | 247.8 / 8.8 | 83 | S226G (AGT→GGT) | narX | sensory histidine kinase in two‑component regulatory system with NarL |
| Reads supporting (aligned to +/- strand): ref base T (44/33); new base C (2/4); total (46/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.00e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01 | |||||||||||
GAGAGGATCTGATTTTTATGCTCCAGCCCGGCGGTTTTCTCCTGAACCCGCTGCTCAAGTACGGCATAACTTTCGGCCAGTTCTGCAGACATATTGTTCAACGCAGTTCCAAGCATCGCCATTTCGTTGCGCCCGCTGATG > minE/828552‑828692 | gagagGATCTGATTTTTATGCTCCAGCCCGGCGGTTTTCTCCTGAACCCGCTGCTCAAGTACGGCATAACt > 1:27178/1‑71 (MQ=255)gagagGATCTGATTTTTATGCTCCAGCCCGGCGGTTTTCTCCTGAACCCGCTGCTCAAGTACGGCATAACt < 1:2817854/71‑1 (MQ=255)gagagGATCTGATTTTTATGCTCCAGCCCGGCGGTTTTCTCCTGAACCCGCTGCTCAAGTACGGCATAACt > 1:3978622/1‑71 (MQ=255)gagagGATCTGATTTTTATGCTCCAGCCCGGCGGTTTTCTCCTGAACCCGCTGCTCAAGTACGGCATAACt > 1:4507569/1‑71 (MQ=255) agagGATCTGATTTTTATGCTCCAGCCCGGCGGTTTTCTCCTGAACCCGCTGCTCAAGTACGGCATAACtt < 1:56889/71‑1 (MQ=255) agGATCTGATTTTTATGCTCCAGCCCGGCAGTTTTCTCCTGAACCCGCTGCTCAAGTACGGCATAACtt < 1:3755905/69‑1 (MQ=255) tCTGATTTTTATGCTCCAGCCCGGCGGTTTTCTCCTGAACCCGCTGCTCAAGTACGGCATAACTTTCGGc > 1:4419915/1‑70 (MQ=255) aTTTTTATGCTCCAGCCCGGCGGTTTTCTCCTGAACCCGCTGCTCAAGTACGGCATAACTTTCGGCCAGtt > 1:1270154/1‑71 (MQ=255) aTTTTTATGCTCCAGCCCGGCGGTTTTCTCCTGAACCCGCTGCTCAAGTACGGCATAACTTTCGGCCAGtt > 1:4115679/1‑71 (MQ=255) cTCCAGCCCGGCGGTTTTCTCCTGAACCCGCTGCTCAAGTACGGCATAACTTTCGGCCAGTTCTGCAGACa > 1:1813071/1‑71 (MQ=255) tCCAGCCCGGCGGTTTTCTCCTGAACCCGCTGCTCAAGTACGGCATAACTTTCGGCCAGTTCTGCAGACa > 1:824387/1‑70 (MQ=255) cAGCCCGGCGGTTTTCTCCTGAACCCGCTGCTCAAGTACGGCATAACtt < 1:1938362/49‑1 (MQ=255) cAGCCCGGCGGTTTTCTCCTGAACCCGCTGCTCAAGTACGGCATAACTTTCGGCCAGTTCTGCAGACatat > 1:2396701/1‑71 (MQ=255) cccGGCGGTTTTCTCCTGAACCCGCTGCTCAAGTACGGCATAACTTTCGGCCAGTTCTGCAGACATATTGt < 1:3209979/71‑1 (MQ=255) ccGGCGGTTTTCTCCTGAACCCGCTGCTCAAGTACGGCATAACTTTCGGCCAGTTCTGCAGACATATTGtt < 1:3009660/71‑1 (MQ=255) ccGGCGGTTTTCTCCTGAACCCGCTGCTCAAGTACGGCATAACTTTCGGCCAGTTCTGCAGACATATTGtt < 1:3521455/71‑1 (MQ=255) cggTTTTCTCCTGAACCCGCTGCTCAAGTACGGCATAACTTTCGGCCAGTTCTGCAg > 1:3262539/1‑57 (MQ=255) cggTTTTCTCCTGAACCCGCTGCTCAAGTACGGCATAACTTTCGGCCAGTTCTGCAGACATATTGTTCAAc < 1:3342373/71‑1 (MQ=255) ttttCTCCTGAACCCGCTGCTCAAGTACGGCATAACTTTCgg < 1:235486/42‑1 (MQ=255) ttttCTCCTGAACCCGCTGCTCAAGTACGGCATAACTTTCgg < 1:736516/42‑1 (MQ=255) ttttCTCCTGAACCCGCTGCTCAAGTACGGCATAACTTTCGGc < 1:1692260/43‑1 (MQ=255) ttttCTCCTGAACCCGCTGCTCAAGTACGGCATAACTTTCGGc < 1:416940/43‑1 (MQ=255) ttttCTCCTGAACCCGCTGCTCAAGTACGGCATAACTTTCGGCCAGTTCTGCAGACATATTGTTCAACg > 1:15457/1‑69 (MQ=255) tttCTCCTGAACCCGCTGCTCAAGTACGGCATAACTTTCGGCCAGTTCTGCAGACATATTGTTCAACGCa > 1:941332/1‑70 (MQ=255) ttCTCCTGAACCCGCTGCTCAAGTACGGCATAACTTTCGGCCa > 1:964960/1‑43 (MQ=255) ttCTCCTGAACCCGCTGCTCAAGTACGGCATAACTTTCGGCCa > 1:29379/1‑43 (MQ=255) ttCTCCTGAACCCGCTGCTCAAGTACGGCATAACTTTCGGCCAGTTCTGCAGACATATTGTTCAACGCAGt > 1:1960156/1‑71 (MQ=255) ttCTCCTGAACCCGCTGCTCAAGTACGGCATAACTTTCGGCCAGTTCTGCAGACATATTGTTCAACGCAGt > 1:123981/1‑71 (MQ=255) ttCTCCTGAACCCGCTGCTCAAGTACGGCATAACTTTCGGCCAGTTCTGCAGACATATTGTTCAACGCAGt > 1:2596554/1‑71 (MQ=255) cTGAACCCGCTGCTCAAGTACGGCATAACTTTCGGCCAGTTCTGCAg < 1:1618684/47‑1 (MQ=255) cTGAACCCGCTGCTCAAGTACGGCATAACTTTCGGCCAGTTCTGCAg < 1:1472128/47‑1 (MQ=255) cTGAACCCGCTGCTCAAGTACGGCATAACTTTCGGCCAGTTCTGCAg < 1:1308715/47‑1 (MQ=255) cTGAACCCGCTGCTCAAGTACGGCATAACTTTCGGCCAGTTCTGCAGACATATTGTTCAACGCAGTTc < 1:1492957/68‑1 (MQ=255) cTGAACCCGCTGCTCAAGTACGGCATAACTTTCGGCCAGTTCTGCAGACATATTGTTCAACGCAGTTCCaa < 1:4591372/71‑1 (MQ=255) cTGAACCCGCTGCTCAAGTACGGCATAACTTTCGGCCAGTTATGCAg < 1:1142153/47‑1 (MQ=255) cTGAACCCGCTGCTCAAGTACGGAATAACTTTCGGCCAGTTCTGCAGACATATTGTTCAACGCAGTTc < 1:4323864/68‑1 (MQ=255) aaCCCGCTGCTCAAGTACGGCATAACTTTCGGCCAGTTCTGCAGACATATTGTTCAACGCAGTTCCAAGc < 1:429943/70‑1 (MQ=255) aaCCCGCTGCTCAAGTACGGCATAACTTTCGGCCAGTTCTGCAGACATATTGTTCAACGCAGTTCCAAGc < 1:3801083/70‑1 (MQ=255) aaCCCGCTGCTCAAGTACGGCATAACTTTCGGCCAGTTCTGCAGACATATTGTTCAACGCAGTTCCAAGc < 1:2044496/70‑1 (MQ=255) aCCCGCTGCTCAAGTACGGCATAACTTTCGGCCAGTTCTGCAGACATATTGTTCAACGCAGTTCCAAGCAt > 1:4512603/1‑71 (MQ=255) aCCCGCTGCTCAAGTACGGCATAACTTTCGGCCAGTTCTGCAGACATATTGTTCAACGCAGTTCCAAGCAt > 1:2924335/1‑71 (MQ=255) cccGCTGCTCAAGTACTGCATAACTTTCGGCCAGTTCTGCAGACATATTGTTCAACGCTGTTc < 1:3800756/63‑1 (MQ=255) ctgctCAAGTACGGCATAACCTTCGGCCAGTTCTGCAGACATATTGTTc < 1:4736915/49‑1 (MQ=255) ctgctCAAGTACGGCATAACCTTCGGCCAGTTCTGCAGACATATTGTTc < 1:479315/49‑1 (MQ=255) ctgctCAAGTACGGCATAACCTTCGGCCAGTTCTGCAGACATATTGTTc < 1:149728/49‑1 (MQ=255) ctgctCAAGTACGGCATAACCTTCGGCCAGTTCTGCAGACATATTGTTc < 1:3293539/49‑1 (MQ=255) gctCAAGTACGGCATAACCTTCGGCCAGTTCTGCAGACATATTGTTCaa > 1:2129665/1‑49 (MQ=255) gctCAAGTACGGCATAACCTTCGGCCAGTTCTGCAGACATATTGTTCaa > 1:3723831/1‑49 (MQ=255) cAAGTACGGCATAACTTTCGGCCAGTTCTGCAGACATATTGTTCAACGCAGTTCCAAGCATCGCCAttt > 1:895466/1‑69 (MQ=255) aaGTACGGCATAACTTTCGGCCAGTTCTGCAGACATATTGTTCAACGCAGTTCCAAGCATCGCCATTTc < 1:4617135/69‑1 (MQ=255) aaGTACGGCATAACTTTCGGCCAGTTCTGCAGACATATTGTTCAACGCAGTTCCAAGCATCGCCATTTCGt < 1:3727376/71‑1 (MQ=255) tACGGCATAACTTTCGGCCAGTTCTGCAGACATATTGTTCAACGCAGTTCCAAGCATCGCCATTTCGTTgc > 1:795715/1‑71 (MQ=255) tACGGCATAACTTTCGGCCAGTTCTGCAGACATATTGTTCAACGCAGTTCCAAGCATCGCCATTTCGTTgc > 1:1773038/1‑71 (MQ=255) tACGGCATAACTTTCGGCCAGTTCTGCAGACATATTGTTCAACGCAGTTCCAAGCATCGCCATTTCGTTgc > 1:4685525/1‑71 (MQ=255) tACGGCATAACTTTCGGCCAGTTCTGCAGACATATTGTTCAACGCAGTTCCAAGCATCGCCATTTCGTTgc > 1:2343693/1‑71 (MQ=255) tACGGCATAACTTTCGGCCAGTTCGGCAGACATATTGTTCAACGCAGTTCCAAGCATCGCCATTTCGTTgc > 1:4245713/1‑71 (MQ=255) cGGCATAACTTTCGGCCAGTTCTGCAGACATATTGTTCAACGCAg > 1:3148000/1‑45 (MQ=255) cGGCATAACTTTCGGCCAGTTCTGCAGACATATTGTTCAACGCAg > 1:2806507/1‑45 (MQ=255) cGGCATAACTTTCGGCCAGTTCTGCAGACATATTGTTCAACGCAg > 1:3036477/1‑45 (MQ=255) cGGCATAACTTTCGGCCAGTTCTGCAGACATATTGTTCAACGCAg > 1:4312789/1‑45 (MQ=255) cGGCATAACTTTCGGCCAGTTCTGCAGACATATTGTTCAACGCAGTTCCAAGCATCGCCAttt > 1:2391817/1‑63 (MQ=255) cGGCATAACTTTCGGCCAGTTCTGCAGACATATTGTTCAACGCAGTTCCAAGCATCGCCAttt > 1:1911661/1‑63 (MQ=255) cGGCATAACTTTCGGCCAGTTCTGCAGACATATTGTTCAACGCAGTTCCAAGCATCGCCAtta > 1:3683531/1‑62 (MQ=255) cGGCATAACTTTCGGCCAGTTCTGCAGACATATTGTTCAACGCAGTTCCAAGCATCGCCATTTCGTTgcgc > 1:779048/1‑71 (MQ=255) ggCATAACTTTCGGCCAGTTCTGCAGACATATTGTTCAACGCAGTTcc > 1:3303711/1‑48 (MQ=255) ggCATAACTTTCGGCCAGTTCTGCAGACATATTGTTCAACGCAGTTcc > 1:1637680/1‑48 (MQ=255) ggCATAACTTTCGGCCAGTTCTGCAGACATATTGTTCAACGCAGTTcc > 1:1895110/1‑48 (MQ=255) ggCATAACTTTCGGCCAGTTCTGCAGACATATTGTTCAACGCAGTTcc > 1:730773/1‑48 (MQ=255) ggCATAACTTTCGGCCAGATCTGCAGACATATTGTTCAACGCAGTTcc > 1:2082499/1‑48 (MQ=255) cATAACTTTCGGCCAGTTCTGCAGACATATTGTTCAACGc < 1:1839719/40‑1 (MQ=255) cATAACTTTCGGCCAGTTCTGCAGACATATTGTTCAACGc < 1:2953598/40‑1 (MQ=255) cATAACTTTCGGCCAGTTCTGCAGACATATTGTTCAACGc < 1:2968357/40‑1 (MQ=255) cATAACTTTCGGCCAGTTCTGCAGACATATTGTTCAACGc < 1:3530096/40‑1 (MQ=255) tAACTTTCGGCCAGTTCTGCAGACATATTGTTCAACGCAGTTCCAAGCa < 1:2973315/49‑1 (MQ=255) tAACTTTCGGCCAGTTCTGCAGACATATTGTTCAACGCAGTTCCAAGCa < 1:3509164/49‑1 (MQ=255) tAACTTTCGGCCAGTTCTGCAGACATATTGTTCAACGCAGTTCCAAGCa < 1:2044824/49‑1 (MQ=255) tAACTTTCGGCCAGTTCTGCAGACATATTGTTCAACGCAGTTCCAAGCATCGCCATTTc < 1:553165/59‑1 (MQ=255) tAACTTTCGGCCAGTTCTGCAGACATATTGTTCAACGCAGTTCCAAGCATCGCCATTTc < 1:1126989/59‑1 (MQ=255) tAACTTTCGGCCAGTTCTGCAGACATATTGTTCAACGCAGTTCCAAGCATCGCCATTTc < 1:1823974/59‑1 (MQ=255) tAACTTTCGGCCAGTTCTGCAGACATATTGTTCAACGCAGTTCCAAGCATCGCCATTTc < 1:3766116/59‑1 (MQ=255) tAACTTTCGGCCAGTTCTGCAGACATATTGTTCAACGCAGTTCCAAGCATCGCCATTTc < 1:2102164/59‑1 (MQ=255) tAACTTTCGGCCAGTTCTGCAGACATATTGTTCAACGCAGTTCCAAGCATCGCCATTTCGTTGCGCCCGCt > 1:4497773/1‑71 (MQ=255) tAACTTTCGGCCAGTTCTGCAGACATATTGTTCAACGCAGTTCCAAGCATCGCCATTTCGTTGCGCCCGCt > 1:50236/1‑71 (MQ=255) tAACTTTCGGCCAGTTCTGCAGACATATTGTTCAACGCAGTTCCAAGCATCGCCATTTCGTTGCGCCCGCt > 1:1338051/1‑71 (MQ=255) tAACTTTCGGCCAGTTCTGCAGACATATTGTTCAACGCAGTTCCAAGCATCGCCATTTCGTTGCGCCCGCt > 1:2576984/1‑71 (MQ=255) aCTTTCGGCCAGTTCTGCAGACATATTGTTCAACGCAGTTCCAAGCATCGCCATTTCGt > 1:1247356/1‑59 (MQ=255) aCTTTCGGCCAGTTCTGCAGACATATTGTTCAACGCAGTTCCAAGCATCGCCATTTCGTTGCGCCCGCTGa > 1:2673159/1‑71 (MQ=255) cTTTCGGCCAGTTCTGCAGACATATTGTTCAACGCAGTTCCAAGCATCGCCATTTCGTTgc > 1:1439952/1‑61 (MQ=255) cTTTCGGCCAGTTCTGCAGACATATTGTTCAACGCAGTTCCAAGCATCGCCATTTCGTTGCGCCCGCTGAt > 1:4316319/1‑71 (MQ=255) cTTTCGGCAAGTTCTGCAGACATATTGTTCAACGCAGTTCCAAGCATCGACATTTCGTTGCGccc > 1:512184/1‑65 (MQ=255) tttCGGCCAGTTCTGCAGACATATTGTTCAACGCAGTTCCAAGCATCGCCATTTCGTTGCGCCCGCTGATg > 1:4718846/1‑71 (MQ=255) tttCGGCCAGTTCTGCAGACATATTGTTCAACGCAGTTCCAAGCATCGCCATTTCGTTGCGCCCGCTGATg > 1:2013857/1‑71 (MQ=255) tttCGGCCAGTTCTGCAGACATATTGTTCAACGCAGTTCCAAGCATCGCCATTTCGTTGCGCCCGCTGATg > 1:3744977/1‑71 (MQ=255) tttCGGCCAGTTATGCAGACATATTGTTCAACGCAGTTCCAAGCATCGCCATTTCGTTGCGCCCGCTGATg > 1:2425234/1‑71 (MQ=255) | GAGAGGATCTGATTTTTATGCTCCAGCCCGGCGGTTTTCTCCTGAACCCGCTGCTCAAGTACGGCATAACTTTCGGCCAGTTCTGCAGACATATTGTTCAACGCAGTTCCAAGCATCGCCATTTCGTTGCGCCCGCTGATG > minE/828552‑828692 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |