| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 863,792 | T→A | 6.3% | Y31* (TAT→TAA) ‡ | yciQ → | predicted inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 863,792 | 0 | T | A | 6.3% | 236.8 / 6.7 | 79 | Y31* (TAT→TAA) ‡ | yciQ | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base T (31/43); new base A (3/2); total (34/45) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.47e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.71e-01 | |||||||||||
TTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAA > minE/863722‑863861 | ttttGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTAt > 1:1785746/1‑71 (MQ=255)ttttGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTAt > 1:435405/1‑71 (MQ=255) ttGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATg < 1:4531384/70‑1 (MQ=255) ttGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATg > 1:1769590/1‑70 (MQ=255) tGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATg > 1:215396/1‑69 (MQ=255) gCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATg > 1:1225798/1‑68 (MQ=255) cTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATgaag < 1:285349/70‑1 (MQ=255) ttGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAgg > 1:1696408/1‑71 (MQ=255) ttGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAgg > 1:2065495/1‑71 (MQ=255) tAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGAtc > 1:1590703/1‑71 (MQ=255) gCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGAtctc > 1:2046183/1‑71 (MQ=255) cAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGAtctc > 1:220302/1‑70 (MQ=255) cAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCg > 1:4117445/1‑71 (MQ=255) aGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCg < 1:2007772/70‑1 (MQ=255) aGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCg < 1:1889471/70‑1 (MQ=255) tttGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGaagaag < 1:3138625/55‑1 (MQ=255) tttGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGaagaag < 1:4417079/55‑1 (MQ=255) tttGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTg < 1:1280187/70‑1 (MQ=255) tGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATg > 1:3393488/1‑71 (MQ=255) tATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGtt < 1:306970/71‑1 (MQ=255) tcatcTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTCATAAGAAgg < 1:3494380/50‑1 (MQ=25) tcatcTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTCATAAGAAgg < 1:1781082/50‑1 (MQ=25) tcatcTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTg < 1:4247866/70‑1 (MQ=255) tcatcTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTg < 1:574056/70‑1 (MQ=255) tcatcTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGa < 1:3635176/71‑1 (MQ=255) tcatcTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGa < 1:3781643/71‑1 (MQ=255) tcatcTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGa < 1:1603042/71‑1 (MQ=255) tcatcTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGa < 1:4410732/71‑1 (MQ=255) tcatcTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGa < 1:699198/71‑1 (MQ=255) tcatcTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGa < 1:1019086/71‑1 (MQ=255) atcTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTCATAAGAAGGGa > 1:270836/1‑50 (MQ=21) atcTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTCATAAGAAGGGa > 1:3087692/1‑50 (MQ=21) atcTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTCATAAGAAGGGa > 1:829399/1‑50 (MQ=21) atcTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAg > 1:1666873/1‑71 (MQ=255) atcTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAg > 1:4423694/1‑71 (MQ=255) tAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCa < 1:4261111/69‑1 (MQ=255) tAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCa < 1:2587104/69‑1 (MQ=255) tAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCa < 1:2632304/69‑1 (MQ=255) tAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCa < 1:1884001/69‑1 (MQ=255) tAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCAct > 1:2234809/1‑71 (MQ=255) aGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGa > 1:2750890/1‑56 (MQ=255) aGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGa > 1:2995220/1‑56 (MQ=255) aGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCActct < 1:4695120/71‑1 (MQ=255) aGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCAct < 1:640971/69‑1 (MQ=255) aGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCAActct > 1:2307132/1‑71 (MQ=255) gTTATGCAGAAAACACGGAGATCCCTTCTTATgaaga < 1:4407412/37‑1 (MQ=255) ttATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTg > 1:2616481/1‑70 (MQ=255) tATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGc > 1:3133113/1‑70 (MQ=255) tATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGc > 1:1385211/1‑70 (MQ=255) aTGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCaa > 1:4431598/1‑71 (MQ=255) aTGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCGTTGATGTTGAAGCCACTCTGCaa > 1:1155152/1‑71 (MQ=255) cAGAAAACACGGAGATCCCTTCTTATGTAGAAGGGATCTCGCTCTTTGATGTTGa > 1:2020278/1‑55 (MQ=255) cAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCa < 1:1108434/71‑1 (MQ=255) aGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAg < 1:3976209/71‑1 (MQ=255) gAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAg < 1:4383889/70‑1 (MQ=255) aaCACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATgg > 1:2178086/1‑71 (MQ=255) cacGGAGATCCCTTCTTATTAAGAAGGGATCTCGCTCTTTGATGTTGa < 1:1272243/48‑1 (MQ=255) cacGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGa < 1:1640396/48‑1 (MQ=255) gagaTCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCAct < 1:1833316/51‑1 (MQ=255) aTCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCa < 1:1289934/57‑1 (MQ=255) aTCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCa < 1:3627075/57‑1 (MQ=255) aTCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCa < 1:1506010/57‑1 (MQ=255) tCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGAc < 1:369722/71‑1 (MQ=255) tCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGAc < 1:2320173/71‑1 (MQ=255) cccTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCa < 1:2176745/55‑1 (MQ=255) cccTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCa < 1:28689/55‑1 (MQ=255) cccTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCa < 1:2210202/55‑1 (MQ=255) ccTTCTTATGAAGAAGTTATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCa < 1:262598/54‑1 (MQ=255) ccTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAg < 1:3163966/55‑1 (MQ=255) ttcttATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATggg < 1:1653596/58‑1 (MQ=255) ttcttATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATc > 1:1456205/1‑70 (MQ=255) cttATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCa > 1:3547894/1‑69 (MQ=255) ttATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGa < 1:1652286/63‑1 (MQ=255) ttATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGa < 1:3327521/63‑1 (MQ=255) ttATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGa > 1:4398449/1‑63 (MQ=255) ttATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGa < 1:3824416/63‑1 (MQ=255) ttATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGa > 1:4738369/1‑63 (MQ=255) aTGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATg > 1:1463483/1‑51 (MQ=255) aTGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATg > 1:4130459/1‑51 (MQ=255) aTGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAg > 1:1100416/1‑69 (MQ=255) aTGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGa > 1:3336499/1‑70 (MQ=255) tGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGaa < 1:518666/70‑1 (MQ=255) tGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGaa < 1:3871669/70‑1 (MQ=255) | TTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAA > minE/863722‑863861 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |