| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 874,334 | T→C | 6.3% | pseudogene (221/299 nt) pseudogene (111/189 nt) |
yciX → yciX → |
ECK1270:JW5198+JW5199:b4574; hypothetical protein ECK1270:JW5199:b4523; hypothetical protein, C‑ter fragment |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 874,334 | 0 | T | C | 6.3% | 336.9 / 9.3 | 111 | pseudogene (221/299 nt) pseudogene (111/189 nt) | yciX yciX | ECK1270:JW5198+JW5199:b4574; hypothetical protein ECK1270:JW5199:b4523; hypothetical protein, C‑ter fragment |
| Reads supporting (aligned to +/- strand): ref base T (54/50); new base C (5/2); total (59/52) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.45e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 116 total aligned reads displayed.
GGAGTCGTCACCATTATGCCAGCATAGTGACAATGAAACAGAAACGAAACGGGAATGTTCCGTCGTTATTCCAGACGACTGGCAACTAACATCGCAGCAGCAAGCCTTTATAGAACTGTTTGCTGAAGATGATCAGCCG > minE/874265‑874403 | gAGTCGTCACCATTATGCCAGCATAGTGACAATGAAACAGAAACGAAACGGGAATGTTCCGTCGTTATTc < 1:315549/70‑1 (MQ=255) gAGTCGTCACCATTATGCCAGCATAGTGACAATGAAACAGAAACGAAACGGGAATGTTCCGTCGTTATTc < 1:4665504/70‑1 (MQ=255) gtcACCATTATGCCAGCATAGTGACAATGAAACAGAAACGAAACGGGAATGTTCCGTCGTTATTCCAga > 1:2129278/1‑69 (MQ=255) aCCATTATGCCAGCATAGTGACAATGAAACAGAAACGAAACGGGAATGTTCCGTCGTTATTCCAGACGACt > 1:2967585/1‑71 (MQ=255) aCCATTATGCCAGCATAGTGACAATGAAACAGAAACGAAACGGGAATGTTCCGTCGTTATTCCAGACGACt > 1:3772954/1‑71 (MQ=255) aCCATTATGCCAGCATAGTGACAATGAAACAGAAACGAAACGGGAATGTTCCGTCGTTATTCCAGACGACt > 1:3707111/1‑71 (MQ=255) aCCATTATGCCAGCATAGTGACAATGAAACAGAAACGAAACGGGAATGTTCCGTCGTTATTCCAGACGACt > 1:3464918/1‑71 (MQ=255) aCCATTATGCCAGCATAGTGACAATGAAACAGAAACGAAACGGGAATGTTCCGTCGTTATTCCAGACGACt > 1:4645889/1‑71 (MQ=255) aTTATGCCAGCATAGTGACAATGAAACAGAAACGAAACGGGAATGTTCCGTCGTTATTCCAGACGACTGGc < 1:2812646/71‑1 (MQ=255) aTTATGCCAGCATAGTGACAATGAAACAGAAACGAAACGGGAATGTTCCGTCGTTATTCCAGACGACTGGc < 1:1725022/71‑1 (MQ=255) ttATGCCAGCATAGTGACAATGAAACAGAAACGAAACGGGAATGTTCCGTCGTTATTCCAGACGACTGGc > 1:835867/1‑70 (MQ=255) aTGCCAGCATAGTGACAATGAAACAGAAACGAAACGGGAATGTTCCGTCGTTATCCCAGACGACTGGCAAc > 1:312060/1‑71 (MQ=255) aTGCCAGCATAGTGACAATGAAACAGAAACGAAACGGGAATGTTCCGTCGTTATCCCAGACGACTGGCAAc > 1:3757491/1‑71 (MQ=255) gCCAGCATAGTGACAATGAAACAGAAACGAAACGGGAATGTTCCGTCGTTATTCCAGACGACTGGCAACt > 1:2522413/1‑70 (MQ=255) gCCAGCATAGTGACAATGAAACAGAAACGAAACGGGAATGTTCCGTCGTTATTCCAGACGACTGGCAACt > 1:358327/1‑70 (MQ=255) ccAGCATAGTGACAATGAAACAGAAACGAAACGGGAATGTTCCGTCGTTATTCCAGACGACTGGCAACTaa < 1:4345783/71‑1 (MQ=255) cAGCATAGTGACAATGAAACAGAAACGAAACGGGAATGTTCCGTCGTTATTCCAGACGACTGGCAACTAAc < 1:727790/71‑1 (MQ=255) aTAGTGACAATGAAACAGAAACGAAACGGGAATGTTCCGTCGTTATTCCAGACGACTGGCAACTAACATCg > 1:3226517/1‑71 (MQ=255) tAGTGACAATGAAACAGAAACGAAACGGGAATGTTCCGTCGTTATTCCAGACGACTGGCAACTAACATCgc < 1:2891336/71‑1 (MQ=255) tAGTGACAATGAAACAGAAACGAAACGGGAATGTTCCGTCGTTATTCCAGACGACTGGCAACTAACATCgc < 1:756413/71‑1 (MQ=255) gTGACAATGAAACAGAAACGAAACGGGAATGTTCCGTCGTTATTCCAGACGACTGGCAACTAACATCgcag > 1:4009613/1‑71 (MQ=255) gTGACAATGAAACAGAAACGAAACGGGAATGTTCCGTCGTTATTCCAGACGACTGGCAACTAACATCgcag > 1:2983343/1‑71 (MQ=255) gTGACAATGAAACAGAAACGAAACGGGAATGTTCCGTCGTTATTCCAGACGACTGGCAACTAACATCgcag > 1:918459/1‑71 (MQ=255) gTGACAATGAAACAGAAACGAAACGGGAATGTTCCGTCGTTATTCCAGACGACTGGCAACTAACATCgcag > 1:976774/1‑71 (MQ=255) tGACAATGAAACAGAAACGAAACGGGAATGTTCCGTCGTTAtt < 1:17141/43‑1 (MQ=255) tGACAATGAAACAGAAACGAAACGGGAATGTTCCGTCGTTAtt < 1:4153159/43‑1 (MQ=255) tGACAATGAAACAGAAACGAAACGGGAATGTTCCGTCGTTAtt < 1:2870569/43‑1 (MQ=255) tGACAATGAAACAGAAACGAAACGGGAATGTTCCGTCGTTATTCCAGACGACTGGCAACTAACATCgcagc < 1:2041895/71‑1 (MQ=255) tGACAATGAAACAGAAACGAAACGGGAATGTTCCGTCGTTATTCCAGACGACTGGCAACTAACATCgcagc < 1:1698280/71‑1 (MQ=255) tGACAATGAAACAGAAACGAAACGGGAATGTTCCGTCGTTATTCCAGACGACTGGCAACTAACATCgcagc < 1:2515893/71‑1 (MQ=255) aCAATGAAACAGAAACGAAACGGGAATGTTCCGTCGTTATTCCAGACGACTGGCAACTAACATCgcagcag < 1:3415987/71‑1 (MQ=255) aCAATGAAACAGAAACGAAACGGGAATGTTCCGTCGTTATTCCAGACGACTGGCAACTAACATCgcagca < 1:721245/70‑1 (MQ=255) aCAATGAAACAGAAACGAAACGGGAATGTTCCGTCGTTATTCCAGACGACTGGCAACTAACATCgcagca < 1:2968003/70‑1 (MQ=255) cAATGAAACAGAAACGAAACGGGAATGTTCCGTCGTTATTCCAGACGACTGGCAACTAACATCgcagcagc < 1:4123295/71‑1 (MQ=255) cAATGAAACAGAAACGAAACGGGAATGTTCCGTCGTTATTCCAGACGACTGGCAACTAACATCgcagca < 1:4538716/69‑1 (MQ=255) aaTGAAACAGAAACGAAACGGGAATGTTCCGTCGTTATTCCa > 1:4286111/1‑42 (MQ=255) aaTGAAACAGAAACGAAACGGGAATGTTCCGTCGTTATTCCa > 1:1038758/1‑42 (MQ=255) aaTGAAACAGAAACGAAACGGGAATGTTCCGTCGTTATTCCa > 1:1873547/1‑42 (MQ=255) aaTGAAACAGAAACGAAACGGGAATGTTCCGTCGTTATTCCa > 1:2890498/1‑42 (MQ=255) aTGAAACAGAAACGAAACGGGAATGTTCCGTCGTTATTc < 1:2694930/39‑1 (MQ=255) aTGAAACAGAAACGAAACGGGAATGTTCCGTCGTTATTc < 1:4498264/39‑1 (MQ=255) aTGAAACAGAAACGAAACGGGAATGTTCCGTCGTTATTc < 1:3072250/39‑1 (MQ=255) aTGAAACAGAAACGAAACGGGAATGTTCCGTCGTTATTc < 1:1562322/39‑1 (MQ=255) aTGAAACAGAAACGAAACGGGAATGTTCCGTCGTTATTc < 1:973019/39‑1 (MQ=255) aTGAAACAGAAACGAAACGGGAATGTTCCGTCGTTATTCCAGACGACTGGCAACTAACATCGCAGCAGCaa < 1:3744177/71‑1 (MQ=255) aTGAAACAGAAACGAAACGGGAATGTTCCGTCGTTATTCCAGACGACTGGCAACTAACATCGCAGCAGCaa < 1:2786436/71‑1 (MQ=255) aTGAAACAGAAACGAAACGGGAATGTTCCGTCGTTATTCCAGACGACTGGCAACTAACATCGCAGCAGCaa < 1:4454520/71‑1 (MQ=255) aTGAAACAGAAACGAAACGGGAATGTTCCGTCGTTATTCCAGACGACTGGCAACTAACATCGCAGCAGCaa < 1:2182049/71‑1 (MQ=255) tGAAACAGAAACGAAACGGGAATGTTCCGTCGTTATTCCAGACGACTGGCAACTAACATCGCAGCAGCAAg < 1:564856/71‑1 (MQ=255) gAAACAGAAACGAAACGGGAATGTTCCGTCGTTATTCCAGACGACTGGCAACTAACATCGCAGCAGCaa > 1:4330664/1‑69 (MQ=255) aaaCAGAAACGAAACGGGAATGTTCCGTCGTTATTCCAGACGACTGGCAACTAACATCGCAGCAGCAAGc < 1:3955953/70‑1 (MQ=255) aaaCAGAAACGAAACGGGAATGTTCCGTCGTTATTCCAGACGACTGGCAACTAACATCGCAGCAGCAAGc < 1:1703405/70‑1 (MQ=255) aaCAGAAACGAAACGGGAATGTTCCGTCGTTATTCCAgacgac > 1:1201769/1‑43 (MQ=255) aaCAGAAACGAAACGGGAATGTTCCGTCGTTATTCCAgacgac > 1:982151/1‑43 (MQ=255) aaCAGAAACGAAACGGGAATGTTCCGTCGTTATTCCAgacgac > 1:3651101/1‑43 (MQ=255) aaCAGAAACGAAACGGGAATGTTCCGTCGTTATTCCAgacgac > 1:1074911/1‑43 (MQ=255) aGAAACGAAACGGGAATGTTCCGTCGTTATTCCAGACGACTGGCAACTAACATCgcagc < 1:4518048/59‑1 (MQ=255) aGAAACGAAACGGGAATGTTCCGTCGTTATTCCAGACGACTGGCAACTAACATCGCAGCAGCAAGCCTTta < 1:935600/71‑1 (MQ=255) aacgaaacgGGAATGTTCCGTCGTTATCCCAGACGACTGGCAACTAACATCGCAGCAGCAAGCCTTTATAg < 1:1717726/71‑1 (MQ=255) acgaaacgGGAATGTTCCGTCGTTATTCCAGACGACTGGCAACTAAc > 1:306858/1‑47 (MQ=255) acgaaacgGGAATGTTCCGTCGTTATTCCAGACGACTGGCAACTAAc > 1:2505157/1‑47 (MQ=255) acgaaacgGGAATGTTCCGTCGTTATTCCAGACGACTGGCAACTAACATCGCAGCAGCaa < 1:541357/60‑1 (MQ=255) cgaaacgGGAATGTTCCGTCGTTATTCCAGACGACTGGCAACTAACATCGCAGCAGCAAGCCTTTATAGaa > 1:3736394/1‑71 (MQ=255) cgaaacgGGAATGTTCCGTCGTTATTCCAGACGACTGGCAACTAACATCGCAGCAGCAAGCCTTTATAGaa > 1:1468818/1‑71 (MQ=255) cgaaacgGGAATGTTCCGTCGTTATTCCAGACGACTGGCAACTAACATCGCAGCAGCAAGCCTTTATAGaa > 1:3213927/1‑71 (MQ=255) aaacgGGAATGTTCCGTCGTTATTCCAGACGACTGGCaa > 1:2618660/1‑39 (MQ=255) aacgGGAATGTTCCGTCGTTATTCCAGACGACTGGCAACTAACATCGCAGCAGCAAGCCTTTATAGAACTg < 1:4723008/71‑1 (MQ=255) aacgGGAATGTTCCGTCGTTATTCCAGACGACTGGCAACTAACATCGCAGCAGCAAGCCTTTATAGAACTg > 1:4330163/1‑71 (MQ=255) aacgGGAATGTTCCGTCGTTATTCCAGACGACTGGCAACTAACATCGCAGCAGCAAGCCTTTATAGAACTg > 1:4221391/1‑71 (MQ=255) aacgGGAATGTTCCGTCGTTATTCCAGACGACTGGCAACTAACATCGCAGCAGCAAGCCTTTATAGAACTg > 1:1529822/1‑71 (MQ=255) acgGGAATGTTCCGTCGTTATTCCAGACGACTGGCAACTAACATCGCAGCAGCAAGCCTTTATAGAACTGt > 1:4338402/1‑71 (MQ=255) cgGGAATGTTCCGTCGTTATTCCAGACGACTGGCAACTAACATc < 1:2625307/44‑1 (MQ=255) cgGGAATGTTCCGTCGTTATTCCAGACGACTGGCAACTAACATc < 1:917619/44‑1 (MQ=255) cgGGAATGTTCCGTCGTTATTCCAGACGACTGGCAACTAACATCGCAGCAGCAAGCCTTTATAGAACTGtt < 1:650150/71‑1 (MQ=255) cgGGAATGTTCCGTCGTTATTCCAGACGACTGGCAACTAACATCGCAGCAGCAAGCCTTTATAGAACTGtt < 1:859571/71‑1 (MQ=255) gggAATGTTCCGTCGTTATTCCAGACGACTGGCAACTAACATCGCAGCAGCAAGCCTTTATAGAACTGttt > 1:4544126/1‑71 (MQ=255) gggAATGTTCCGTCGTTATTCCAGACGACTGGCAACTAACATCGCAGCAGCAAGCCTTTATAGAACTGtt < 1:364867/70‑1 (MQ=255) aTGTTCCTTCGTTATTCCAGACGACTGGCAACTAACATCGCAGCAGCAAGcc > 1:1135968/1‑52 (MQ=255) aTGTTCCGTCGTTATTCCAGACGACTGGCAACTAACATCGCAGCAGCAAGcc > 1:4452701/1‑52 (MQ=255) aTGTTCCGTCGTTATTCCAGACGACTGGCAACTAACATCGCAGCAGCAAGcc > 1:305805/1‑52 (MQ=255) aTGTTCCGTCGTTATTCCAGACGACTGGCAACTAACATCGCAGCAGCAAGcc > 1:1034512/1‑52 (MQ=255) ttCCGTCGTTATTCCAGACGACTGGCAACTAACATCGCAGCAGCAAGCCTTTATAGAACTGTTTGCTg > 1:1552331/1‑68 (MQ=255) ttCCGTCGTTATTCCAGACGACTGGCAACTAACATCGCAGCAGCAAGCCTTTATAGAACTGTTTGCTg > 1:2135540/1‑68 (MQ=255) ttCCGTCGTTATTCCAGACGACTGGCAACTAACATCGCAGCAGCAAGCCTTTATAGAACTGTTTGCTg > 1:1469150/1‑68 (MQ=255) ttCCGTCGTTATTCCAGACGACTGGCAACTAACATCGCAGCAGCAAGCCTTTATAGAACTGTTTGCTGAAg < 1:1559934/71‑1 (MQ=255) ttCCGTCGTTATTCCAGACGACTGGCAACTAACATCGCAGCAGCAAGCCTTTATAGAACTGTTTGCTGAAg < 1:2844832/71‑1 (MQ=255) ttCCGTCGTTATCCCAGACGACTGGCAACTAACATCGCAGCAGCAAGcc > 1:4577731/1‑49 (MQ=255) ttCCGTCGTTATCCCAGACGACTGGCAACTAACATCGCAGCAGCAAGcc > 1:2446936/1‑49 (MQ=255) cgtcgtTATTCCAGACGACTGGCAACTAACATCGCAGCAGCAAGCCTTTATAGAACTGTTTGCTGAAgatg < 1:4014256/71‑1 (MQ=255) gtcgtTATTCCAGACGACTGGCAACTAACATCGCAGCAGCAAGCCTTTATAGAACTGTTTGCTGAAgatga < 1:1364006/71‑1 (MQ=255) gtcgtTATTCCAGACGACTGGCAACTAACATCGCAGCAGCAAGCCTTTATAGAACTGTTTGCTGAAgatga < 1:1331582/71‑1 (MQ=255) tcgtTATTCCAGACGACTGGCAACTAACATCGCAGCAGCAAGCCTTTATAGAACTGtt < 1:4513450/58‑1 (MQ=255) cgtTATTCCAGACGACTGGCAACTAACATCGCAGCAGCAAGCCTTTATAGAACTGTTTGCTGAAgatgat > 1:3167857/1‑70 (MQ=255) gtTATTCCAGACGACTGGCAACTAACATCGCAGCAGCAAGCCTTTATAGAACTGTTTGCTGAAgatgat > 1:4582647/1‑69 (MQ=255) gtTATTCCAGACGACTGGCAACTAACATCGCAGCAGCAAGCCTTTATAGAACTGTTTGCTGAAgatgat > 1:1933518/1‑69 (MQ=255) tATCCCAGACGACTGGCAACTAACATCGCAGCAGCAAGCCTTTATAg < 1:3166850/47‑1 (MQ=255) aTTCCAGACGACTGGCAACTAACATCGCAGCAGCAAGCCTTTATAGAACTGTTTGCTGAAGATGATCAGcc > 1:3593769/1‑71 (MQ=255) aTTCCAGACGACTGGCAACTAACATCGCAGCAGCAAGCCTTTATAGAACTGTTTGCTGAAGATGATCAGc > 1:2078117/1‑70 (MQ=255) ttCCAGACGACTGGCAACTAACATCGCAGCAGCAAGCCTTTATAGAACTGTTTGCTGAAGATGATCAGCCg < 1:3762735/71‑1 (MQ=255) tCCAGACGACTGGCAACTAACATCGCAGCAGCAAGCCTTTATAGAACTGTTTGCTGaa < 1:786180/58‑1 (MQ=255) | GGAGTCGTCACCATTATGCCAGCATAGTGACAATGAAACAGAAACGAAACGGGAATGTTCCGTCGTTATTCCAGACGACTGGCAACTAACATCGCAGCAGCAAGCCTTTATAGAACTGTTTGCTGAAGATGATCAGCCG > minE/874265‑874403 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |