| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 965,646 | C→T | 8.9% | G83D (GGC→GAC) | ydeM ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 965,646 | 0 | C | T | 8.9% | 315.0 / 14.5 | 101 | G83D (GGC→GAC) | ydeM | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (39/53); new base T (3/6); total (42/59) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.32e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 101 total aligned reads displayed.
AAAGGCACACCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAGGCCAGCCAGAGTGGGTTCACC > minE/965579‑965713 | aaaGGCACACCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTg < 1:3624547/71‑1 (MQ=255) aGGCACACCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCa > 1:3797910/1‑71 (MQ=255) aGGCACACCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCa > 1:416174/1‑71 (MQ=255) ggCACACCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCAt < 1:4535664/71‑1 (MQ=255) gCACACCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATa < 1:313503/71‑1 (MQ=255) cacaCCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATAg < 1:3236791/71‑1 (MQ=255) acacCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGc < 1:2191605/66‑1 (MQ=255) acacCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATAGc < 1:3138502/71‑1 (MQ=255) acCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCa > 1:3142641/1‑65 (MQ=255) acCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCAt > 1:4284484/1‑66 (MQ=255) acCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCAt > 1:2224367/1‑66 (MQ=255) acCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATAGCgt < 1:3654183/71‑1 (MQ=255) acCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATAGCgt < 1:3407338/71‑1 (MQ=255) ccATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATAGCgtt > 1:1158337/1‑71 (MQ=255) cattcattATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATAGCgttg < 1:2282/71‑1 (MQ=255) ttcattATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATAg < 1:3437677/64‑1 (MQ=255) ttcattATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATAg < 1:2505698/64‑1 (MQ=255) cattATTCAATAAAATGCCATTAGTTTGTAATGCATTAAAAAAACGTTATTGTCCTGc > 1:2511746/1‑58 (MQ=38) aaTAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGaa > 1:4339535/1‑70 (MQ=255) aTAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGTATGCATAGCGTTGTTGATAGTGAAt < 1:3310844/70‑1 (MQ=255) aTAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATAGCgttg > 1:278473/1‑58 (MQ=255) aTAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATAGCgttg > 1:4703402/1‑58 (MQ=255) tAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTg < 1:3453377/47‑1 (MQ=255) tAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTg < 1:4093703/47‑1 (MQ=255) tAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTg < 1:218818/47‑1 (MQ=255) tAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATaa > 1:2650890/1‑71 (MQ=255) aaaaTGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATAGCGTTGTTGACAGTGAATAAc > 1:2320346/1‑71 (MQ=255) aaaTGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCAt > 1:3173023/1‑48 (MQ=255) aaaTGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCAt > 1:4130173/1‑48 (MQ=255) aaaTGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCAt > 1:3934992/1‑48 (MQ=255) aaaTGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCAt > 1:2240723/1‑48 (MQ=255) aaTGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACtt < 1:4030711/71‑1 (MQ=255) aTGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACttt < 1:805488/71‑1 (MQ=255) tGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTa < 1:4216762/71‑1 (MQ=255) tGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTa < 1:4043756/71‑1 (MQ=255) cATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACgg < 1:554133/71‑1 (MQ=255) ttCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATAg < 1:1553363/42‑1 (MQ=255) ttCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATAGc < 1:304187/43‑1 (MQ=255) tCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATAGCGTTGTTGATa < 1:3742102/52‑1 (MQ=255) tCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATAGCGTTGTTGATa < 1:3100820/52‑1 (MQ=255) cGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATAGCg > 1:3032696/1‑42 (MQ=255) cGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATAGCg > 1:4279856/1‑42 (MQ=255) cGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATAGCGttgttg > 1:2898406/1‑48 (MQ=255) cGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGaaa > 1:1756840/1‑70 (MQ=255) cGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGaaa > 1:3559710/1‑70 (MQ=255) tttGTAATGCATTAAAAATACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGaaaaaa < 1:2630350/71‑1 (MQ=255) tttGTAATGCATTAAAAATACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGaaaaaa < 1:346300/71‑1 (MQ=255) tttGTAATGCATTAAAAATACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGaaaaaa < 1:3818530/71‑1 (MQ=255) tGTAATGCATTAAAAATACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAAt < 1:3122803/70‑1 (MQ=255) gTAATGCATTAAAAATACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATc < 1:4757292/70‑1 (MQ=255) tAATGCATTAAAAATACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATcc < 1:2754532/70‑1 (MQ=255) tAATGCATTAAAAATACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATcc < 1:3498319/70‑1 (MQ=255) aTGCATTAAAAATACGTTTTTGGTCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGaaaaa < 1:1102307/64‑1 (MQ=255) aTGCATTAAAAATACGTTTTTGGTCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGaaaaa < 1:4053097/64‑1 (MQ=255) aTGCATTAAAAATACGTTTTTGGTCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGaaaaa < 1:2013593/64‑1 (MQ=255) aTGCATTAAAAATACGTTTTTGGTCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGaaaaa < 1:1878530/64‑1 (MQ=255) aTGCATTAAAAATACGTTTTTGGTCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGaaaaa < 1:1660388/64‑1 (MQ=255) aTGCATTAAAAATACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAgg > 1:4387277/1‑71 (MQ=255) aTGCATTAAAAATACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAgg > 1:1629943/1‑71 (MQ=255) tGCATTAAAAATACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAGgc < 1:3468674/71‑1 (MQ=255) tGCATTAAAAATACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAGgc < 1:2359000/71‑1 (MQ=255) tGCATTAAAAATACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAGgc < 1:1485514/71‑1 (MQ=255) tGCATTAAAAATACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAGgc < 1:374060/71‑1 (MQ=255) cATTAAAAATACGTTTTTGGCCTGCATAGCGttgttg < 1:3160746/37‑1 (MQ=255) cATTAAAAATACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAGgc > 1:583509/1‑69 (MQ=255) ttAAAAATACGTTTTTGGCCTGCATAGCGTTGTTGATAGTg < 1:3823301/41‑1 (MQ=255) tAAAAATACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAGgccagc > 1:838688/1‑70 (MQ=255) aaaaaTACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGaaaaaa > 1:2860158/1‑58 (MQ=255) aaaaaTACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAGgccagc > 1:3191647/1‑69 (MQ=255) aaaaaTACGTTTTTGGCCTGCACAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAGgccagc > 1:2322061/1‑69 (MQ=255) aaaaTACGTTTTTGGTCTGCATAGCGTTGTTGATa > 1:922253/1‑35 (MQ=255) aaaaTACGTTTTTGGTCTGCATAGCGTTGTTGATa > 1:3236658/1‑35 (MQ=255) aaaaTACGTTTTTGGTCTGCATAGCGTTGTTGATa > 1:3231282/1‑35 (MQ=255) aaaaTACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAGgccagcca < 1:630486/70‑1 (MQ=255) aaaaTACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAGgccagcca < 1:2537879/70‑1 (MQ=255) aaaaTACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAGgccagc > 1:108122/1‑68 (MQ=255) aaaaTACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAGgccagc > 1:3627041/1‑68 (MQ=255) aaaaTACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAGgccagc > 1:3621812/1‑68 (MQ=255) aaaaTACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAGgccagc > 1:1313779/1‑68 (MQ=255) aaaaTACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAGgccagc > 1:151067/1‑68 (MQ=255) aaaaTACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAGgccagc > 1:4628995/1‑68 (MQ=255) aaaaTACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAGGccagccag < 1:3740763/71‑1 (MQ=255) aaaaTACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAGGccagccag < 1:3068130/71‑1 (MQ=255) aaTACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAGGCCAGCCagag > 1:3216239/1‑71 (MQ=255) aTACGTTTTTGGCCTGCATAACGTTGTTGATAGTGAATAACTTTACGGaaaaaa < 1:768553/54‑1 (MQ=255) tACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAGGCCAGCCAGAGTg < 1:2740340/71‑1 (MQ=255) aCGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAGGCCAGCCAGAGTgg > 1:327260/1‑71 (MQ=255) cGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAGGCCAGCCAGAGTggg < 1:4679781/71‑1 (MQ=255) gTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAGGCCAGCCAGAGTGGGt > 1:3825159/1‑71 (MQ=255) gTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAGGCCAGCCAGAGTGGGt < 1:4187411/71‑1 (MQ=255) tttttGGCCTGCATAGCGTTGTTGATAGTGAATAACttt < 1:2681045/39‑1 (MQ=255) tttttGGCCTGCATAGCGTTGTTGATAGTGAATAACttt < 1:1232985/39‑1 (MQ=255) tttttGGCCTGCATAGCGTTGTTGATAGTGAATAACttt < 1:1253689/39‑1 (MQ=255) tttttGGCCTGCATAGCGTTGTTGATAGTGAATAACttt < 1:688739/39‑1 (MQ=255) tttttGGCCTGCATAGCGTTGTTGATAGTGAATAACttt < 1:3177231/39‑1 (MQ=255) tttttGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAGGCCAGCCAGAGTGGGtt > 1:4695323/1‑71 (MQ=255) tttttGGCCTGCATAGAGTTGTTGATAGTGAATAACttt < 1:1641644/39‑1 (MQ=255) tttGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAg > 1:1692874/1‑52 (MQ=255) ggCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAGGCCAGCCAGAGTGGGTTCAcc < 1:2425779/70‑1 (MQ=255) gCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGaaa < 1:4726686/40‑1 (MQ=255) | AAAGGCACACCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAGGCCAGCCAGAGTGGGTTCACC > minE/965579‑965713 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |