BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	974,093	A→G	8.2%	T290A (ACG→GCG)	lsrB →	AI2 transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	974,093	0	A	G	8.2%	280.5 / 14.3	97	T290A (ACG→GCG)	lsrB	AI2 transporter
Reads supporting (aligned to +/- strand): ref base A (45/44); new base G (3/5); total (48/49)
Fisher's exact test for biased strand distribution p-value = 7.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.88e-01

Only 100 of 107 total aligned reads displayed.

ATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGTCTCGCCAAACAGCGTTCAGGGCTATGA  >  minE/974025‑974163

aTGTGGTTCAGCAAGTCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACg                                                                      >  1:3039700/1‑71 (MQ=255)

aTGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACg                                                                      >  1:454018/1‑71 (MQ=255)

aTGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACg                                                                      >  1:1262119/1‑71 (MQ=255)

 tgtgGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACgg                                                                     >  1:2647074/1‑71 (MQ=255)

 tgtgGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACgg                                                                     >  1:4497620/1‑71 (MQ=255)

   tgGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGc                                                                   >  1:3209017/1‑71 (MQ=255)

       tCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGAc                                                                >  1:3958352/1‑70 (MQ=255)

        cAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGTGCGACa                                                               <  1:3815849/70‑1 (MQ=255)

        cAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGAc                                                                >  1:2724709/1‑69 (MQ=255)

        cAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGAc                                                                >  1:3352861/1‑69 (MQ=255)

         aGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACa                                                               <  1:815865/69‑1 (MQ=255)

             aGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAg                                                             >  1:2861803/1‑67 (MQ=255)

                cAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGata                                                      >  1:3081926/1‑71 (MQ=255)

                cAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGata                                                      >  1:1803647/1‑71 (MQ=255)

                cAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGata                                                      >  1:3076254/1‑71 (MQ=255)

                cAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGata                                                      >  1:379921/1‑71 (MQ=255)

                 aaaaTTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGc                                                                   <  1:1595295/57‑1 (MQ=255)

                 aaaaTTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGc                                                                   <  1:3700840/57‑1 (MQ=255)

                 aaaaTTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGc                                                                   <  1:2445336/57‑1 (MQ=255)

                          gtgtATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGt                                            <  1:889775/71‑1 (MQ=255)

                           tgtATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAggg                                               <  1:2006776/67‑1 (MQ=255)

                           tgtATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAggg                                               <  1:2611251/67‑1 (MQ=255)

                             tATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCg                                             >  1:4238276/1‑67 (MQ=255)

                             tATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCg                                             >  1:419780/1‑67 (MQ=255)

                             tATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAgg                                         >  1:3472029/1‑71 (MQ=255)

                             tATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAgg                                         >  1:264501/1‑71 (MQ=255)

                             tATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAgg                                         >  1:577595/1‑71 (MQ=255)

                               tGTCGCGGATGCATTATTGAAAAAAGGATCGATGAAAAc                                                                       >  1:3002479/1‑39 (MQ=255)

                               tGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAAc                                                                       >  1:3760705/1‑39 (MQ=255)

                                 tCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCa                                      <  1:1259202/70‑1 (MQ=255)

                                 tCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCa                                      <  1:2253511/70‑1 (MQ=255)

                                 tCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCa                                      <  1:2320545/70‑1 (MQ=255)

                                   gcgTATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGt                                   >  1:4680572/1‑71 (MQ=255)

                                     ggATGCATTATTGAAAAAAGGATCAATGAAAGCGGGCGACAAGCTGGATATc                                                    <  1:1007436/52‑1 (MQ=255)

                                     ggATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTg                                 <  1:2138040/71‑1 (MQ=255)

                                     ggATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTg                                 <  1:2250880/71‑1 (MQ=255)

                                     ggATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTg                                 <  1:532357/71‑1 (MQ=255)

                                      gATGCATTATTGAAAAAAGGATCAATGAAAGCGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGa                                <  1:376355/71‑1 (MQ=255)

                                      gATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTg                                 <  1:570984/70‑1 (MQ=255)

                                      gATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGa                                <  1:3679590/71‑1 (MQ=255)

                                      gATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGa                                <  1:4358002/71‑1 (MQ=255)

                                      gATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGa                                <  1:532706/71‑1 (MQ=255)

                                       aTGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGaa                               <  1:41332/71‑1 (MQ=255)

                                       aTGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGaa                               <  1:1319552/71‑1 (MQ=255)

                                       aTGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGaa                               <  1:2492933/71‑1 (MQ=255)

                                       aTGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGaa                               <  1:2404728/71‑1 (MQ=255)

                                        tGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAg                              <  1:2211688/71‑1 (MQ=255)

                                           attattGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCa                                      <  1:2659516/60‑1 (MQ=255)

                                            ttattGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATc                                                    <  1:2988457/45‑1 (MQ=255)

                                            ttattGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATc                                                    <  1:206662/45‑1 (MQ=255)

                                            ttattGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATc                                                    <  1:384718/45‑1 (MQ=255)

                                            ttattGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATc                                                    <  1:4270441/45‑1 (MQ=255)

                                            ttattGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAg                                     <  1:1951556/60‑1 (MQ=255)

                                            ttattGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAg                                     <  1:4409717/60‑1 (MQ=255)

                                            ttattGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAg                                     <  1:3312551/60‑1 (MQ=255)

                                             tattGAAAAAAGGATCAATGAAAGCGGGCGACAAGCTGGATATCAAGGGCGTa                                           >  1:3026317/1‑53 (MQ=255)

                                             tattGAAAAAAGGATCAATGAAAGCGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGTCTCg                         >  1:3837444/1‑71 (MQ=255)

                                             tattGAAAAAAGGATCAATGAAAGCGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGTCTCg                         >  1:3175283/1‑71 (MQ=255)

                                              attGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGaa                               >  1:2496202/1‑64 (MQ=255)

                                              attGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGTCTCGc                        >  1:3198833/1‑71 (MQ=255)

                                              attGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGTCTCGc                        >  1:3025854/1‑71 (MQ=255)

                                              attGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGTCTCGc                        >  1:1104572/1‑71 (MQ=255)

                                              attGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGTCTCGc                        >  1:1373691/1‑71 (MQ=255)

                                               ttGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGTCTCGc                        >  1:1662658/1‑70 (MQ=255)

                                               ttGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGTCTCGc                        >  1:1361934/1‑70 (MQ=255)

                                                tGAAAAAAGGATCAATGAAAACGGGCGACAAGCTg                                                          <  1:3710394/35‑1 (MQ=255)

                                                tGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGata                                                      >  1:4322785/1‑39 (MQ=255)

                                                tGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGaa                               >  1:826155/1‑62 (MQ=255)

                                                 gAAAAAAGGATCAATGAAAGCGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGTCTCGCCa                      <  1:4284627/70‑1 (MQ=255)

                                                 gAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGTCTCGCCa                      <  1:2162690/70‑1 (MQ=255)

                                                 gAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGTCTCGCCa                      <  1:1673755/70‑1 (MQ=255)

                                                  aaaaaaGGATCAATGAAAACGGGCGACAAGCTGGa                                                        >  1:2620377/1‑35 (MQ=255)

                                                  aaaaaaGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGTCTCGCCaaa                    <  1:3782155/71‑1 (MQ=255)

                                                    aaaaGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGTCTCGCCAAACa                  <  1:388613/71‑1 (MQ=255)

                                                    aaaaGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGTCTCGCCAAACa                  <  1:1471646/71‑1 (MQ=255)

                                                       aGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGTCTCGCCAAACAGCg               >  1:1628223/1‑71 (MQ=255)

                                                          aTCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGTCTCGCCAAACAGCGtt             >  1:4119429/1‑70 (MQ=255)

                                                          aTCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGTCTCGCCAAACAGCGtt             >  1:3113739/1‑70 (MQ=255)

                                                           tCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGTCTCGCCAAACAGCGtt             >  1:3432203/1‑69 (MQ=255)

                                                           tCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGTCTCGCCAAACAGCGtt             >  1:276687/1‑69 (MQ=255)

                                                           tCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGTCTCGCCAAACAGCGtt             >  1:4374851/1‑69 (MQ=255)

                                                            cAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGTCTCGCCaaa                    >  1:349877/1‑61 (MQ=255)

                                                            cAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGTCTCGCCaaa                    >  1:4696543/1‑61 (MQ=255)

                                                             aaTGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGTCTCGCCAAACAGCGTTCAg          <  1:3073159/70‑1 (MQ=255)

                                                             aaTGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGTCTCGCCAAACAGCGTTCAg          <  1:199376/70‑1 (MQ=255)

                                                             aaTGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGTCTCGCCAAACAGCGTTCAg          <  1:1440692/70‑1 (MQ=255)

                                                               tGAAAGCGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTg                                 <  1:4686044/45‑1 (MQ=255)

                                                               tGAAAGCGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTg                                 <  1:2528235/45‑1 (MQ=255)

                                                               tGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGTCTCGCCa                      >  1:4247177/1‑56 (MQ=255)

                                                               tGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGTCTCGCCa                      >  1:775862/1‑56 (MQ=255)

                                                                 aaagCGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGaa                               >  1:529852/1‑45 (MQ=255)

                                                                 aaaaCGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGTCTCGCCAAACAGCGTTCa           >  1:3379036/1‑65 (MQ=255)

                                                                 aaaaCGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGTCTCGCCAAACAGCGTTCa           >  1:765084/1‑65 (MQ=255)

                                                                 aaaaCGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGTCTCGCCAAACAGCGTTCa           >  1:2502786/1‑65 (MQ=255)

                                                                 aaaaCGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGTCTCGCCAAACAGCGTTCa           >  1:2155924/1‑65 (MQ=255)

                                                                 aaaaCGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGTCTCGCCAAACAGCGTTCAGGGCTa     >  1:2803487/1‑71 (MQ=255)

                                                                 aaaaCGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGTCTCGCCAAACAGCGTTCAGGGCTa     >  1:2695279/1‑71 (MQ=255)

                                                                  aaaCGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGTCTCGCCAAACAGCGTTCAGGGCt      <  1:1886914/69‑1 (MQ=255)

                                                                    aCGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGTCTCGCCAAACAGCGTTCAGGGCTATGa  >  1:4490678/1‑71 (MQ=255)

                                                                    aCGGGCGACAAGATGGATATCAAGGGCGTAGGTCAGGTTGAAGTCTCGCCAAACAGCGTTCAGGGCt      >  1:2359789/1‑67 (MQ=255)

ATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGTCTCGCCAAACAGCGTTCAGGGCTATGA  >  minE/974025‑974163

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`