Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	120,663	C→T	17.1%	C181Y (TGC→TAC)	speE ←	spermidine synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	120,663	0	C	T	17.1%	171.4 / 26.4	70	C181Y (TGC→TAC)	speE	spermidine synthase
Reads supporting (aligned to +/- strand):  ref base C (21/37);  new base T (5/7);  total (26/44)
Fisher's exact test for biased strand distribution p-value = 7.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01

TCGATGGCTTCTTCCTGCTGTAAAAAGCAGACGCCGTTTTGTGCGACGAAGATACCGCCAGGATTCAGGCAACGTTTGCAGCCTTCATAAAATGCCGAAGTGAAAAGGCTTTCGCCGGGACCGATAGGATCGGTG  >  minE/120594‑120728                                                                      |                                                                  tCGATGGCTTCTTCCTGCTGTAAAAAGCAGACGCCGTTTTGTGCGACGAAGATACCGCCAGGATTCAGGCa                                                                  <  1:1455742/71‑1 (MQ=255) tCGATGGCTTCTTCCTGCTGTAAAAAGCAGACGCCGTTTTGTGCGACGAAGATACCGCCAGGATTCAGGCa                                                                  <  1:448788/71‑1 (MQ=255) tCGATGGCTTCTTCCTGCTGTAAAAAGCAGACGACGTTTTGTGCGACGAAGATACCGCCAGGATTCAGGCa                                                                  <  1:19724/71‑1 (MQ=255)   gATGGCTTCTTCCTGCTGTAAAAAGCAGACGCCGTTTTGTGCGACGAAGATACCGCCAGGATTCAGGCAAc                                                                >  1:915239/1‑71 (MQ=255)      ggCTTCTTCCTGCTGTAAAAAGCAGACGCCGTTTTGTGCGACGAAGATACCGCCAGGATTCAGGCAACGtt                                                             >  1:734988/1‑71 (MQ=255)      ggCTTCTTCCTGCTGTAAAAAGCAGACGCCGTTTTGTGCGACGAAGATACCGCCAGGATTCAGGCAACGtt                                                             >  1:303552/1‑71 (MQ=255)      ggCTTCTTCCTGCTGTAAAAAGCAGACGCCGTTTTGTGCGACGAAGATACCGCCAGGATTCAGGCAACGtt                                                             >  1:2560410/1‑71 (MQ=255)        cttcttCCTGCTGTAAAAAGCAGACGCCGTTTTGTGCGACGAAGATACCGCCAGGATTCAGGCAACGTTTg                                                           <  1:1299909/71‑1 (MQ=255)        cttcttCCTGCTGTAAAAAGCAGACGCCGTTTTGTGCGACGAAGATACCGCCAGGATTCAGGCAACGTTTg                                                           <  1:4075110/71‑1 (MQ=255)          tcttcCTGCTGTAAAAAGCAGACGCCGTTTTGTGCGACGAAGATACCGCCAGGATTCAGGCAACGTTTg                                                           <  1:4212367/69‑1 (MQ=255)                     tAAAAAGCAGACGCCGTTTTGTGCGACGAAGATACCGCCAGGATTCAGGCAACGTTTGCAGCCTTCATa                                                <  1:2541484/69‑1 (MQ=255)                     tAAAAAGCAGACGCCGTTTTGTGCGACGAAGATACCGCCAGGATTCAGGCAACGTTTGCAGCCTTCATa                                                <  1:243014/69‑1 (MQ=255)                       aaaaGCAGACGCCGTTTTGTGCGACGAAGATACCGCCAGGATTCAGGTAAc                                                                >  1:2167812/1‑51 (MQ=255)                       aaaaGCAGACGCCGTTTTGTGCGACGAAGATACCGCCAGGATTCAGGCAACGTTTGCAGCCTTCATAAAAt                                            >  1:1346941/1‑71 (MQ=255)                        aaaGCAGACGCCGTTTTGTGCGACGAAGATACCGCCAGGATTCAGGCAACGTTTGCAGCCTTCATAAAATg                                           <  1:1781617/71‑1 (MQ=255)                         aaGCAGACGCCGTTTTGTGCGACGAAGATACCGCCAGGATTCAGGCAACGTTTGCAGCCTTCATAAAATGc                                          >  1:841867/1‑71 (MQ=255)                         aaGCAGACGCCGTTTTGTGCGACGAAGATACCGCCAGGATTCAGGCAACGTTTGCAGCCTTCATAAAATGc                                          >  1:3660687/1‑71 (MQ=255)                          aGCAGACGCCGTTTTGTGCGACGAAGATACCGCCAGGATTCAGGc                                                                   <  1:992445/45‑1 (MQ=255)                          aGCAGACGCCGTTTTGTGCGACGAAGATACCGCCAGGATTCAGGc                                                                   <  1:2495657/45‑1 (MQ=255)                          aGCAGACGCCGTTTTGTGCGACGAAGATACCGCCAGGATTCAGGc                                                                   <  1:3164671/45‑1 (MQ=255)                          aGCAGACGCCGTTTTGTGCGACGAAGATACCGCCAGGATTCAGGc                                                                   <  1:498186/45‑1 (MQ=255)                          aGCAGACGCCGTTTTGTGCGACGAAGATACCGCCAGGATTCAGGc                                                                   <  1:1443105/45‑1 (MQ=255)                          aGCAGACGCCGTTTTGTGCGACGAAGATACCGCCAGGATTCAGGc                                                                   <  1:1632124/45‑1 (MQ=255)                           gCAGACGCCGTTTTGTGCGACGAAGATACCGCCAGGATTCAGGCAACGTTTGCAGCCTTCATAAAATGCCg                                        <  1:2608765/71‑1 (MQ=255)                                 gCCGTTTTGTGCGACGAAGATACCGCCAGGATTCAGGCAACGTTTGCAGCCTTCATAAAATGCCGAAGTGa                                  <  1:614542/71‑1 (MQ=255)                                  ccGTTTTGTGCGACGAAGATACCGCCAGGATTCAGGCAACGTTTg                                                           <  1:2246125/45‑1 (MQ=255)                                  ccGTTTTGTGCGACGAAGATACCGCCAGGATTCAGGCAACGTTTGCAGCCTTCATAAAATGCCGAAGTGaa                                 <  1:1334881/71‑1 (MQ=255)                                   cGTTTTGTGCGACGAAGATACCGCCAGGATTCAGGTAACGTTTGCAGCCTTCATAAAATGCCGAAGTGaaa                                <  1:154184/71‑1 (MQ=255)                                   cGTTTTGTGCGACGAAGATACCGCCAGGATTCAGGCAACGTTTGCAGCCTTCATAAAATGCCGAAGTGaa                                 <  1:1897977/70‑1 (MQ=255)                                     ttttGTGCGACGAAGATACCGCCAGGATTCATGCAACGTTTGCAGCCTTCATAAAATGCCGAAGTGaaaa                               >  1:4204192/1‑70 (MQ=255)                                     ttttGTGCGACGAAGATACCGCCAGGATTCAGGTAACGTTTGCAGCCTTCATAAAATGCCGAAGTGaaa                                >  1:4688320/1‑69 (MQ=255)                                     ttttGTGCGACGAAGATACCGCCAGGATTCAGGCAACGTTTGCAGCCTTc                                                   >  1:795794/1‑50 (MQ=255)                                      tttGTGCGACGAAGATACCGCCAGGATTCAGGCAACGTTTGCAGCCTTCATAAAATGCCGAAGTGaaaa                               >  1:1723966/1‑69 (MQ=255)                                      tttGTGCGACGAAGATACCGCCAGGATTCAGGCAACGTTTGCAGCCTTCATAAAATGCCGAAGTGAAAAgg                             >  1:2807694/1‑71 (MQ=255)                                       ttGTGCGACGAAGATACCGCCAGGATTCAGGCAACGTTTGCAGCCTTCATaaaa                                             >  1:1149145/1‑54 (MQ=255)                                       ttGTGCGACGAAGATACCGCCAGGATTCAGGCAACGTTTGCAGCCTTCATaaaa                                             >  1:2484491/1‑54 (MQ=255)                                       ttGTGCGACGAAGATACCGCCAGGATTCAGGCAACGTTTGCAGCCTTCATAAAATGCCGAAGTGAAAAgg                             <  1:3459159/70‑1 (MQ=255)                                       ttGTGCGACGAAGATACCGCCAGGATTCAGGCAACGTTTGCAGCCTTCATAAAATGCCGAAGTGAAAAgg                             <  1:937648/70‑1 (MQ=255)                                         gtgCGACGAAGATACCGCCAGGATTCAGGCAACGTTTGCAGCCTTCATAAAATGCCg                                        <  1:2006996/57‑1 (MQ=255)                                         gtgCGACGAAGATACCGCCAGGATTCAGGCAACGTTTGCAGCCTTCATAAAATGCCg                                        <  1:3387670/57‑1 (MQ=255)                                         gtgCGACGAAGATACCGCCAGGATTCAGGCAACGTTTGCAGCCTTCATAAAATGCCg                                        <  1:4552101/57‑1 (MQ=255)                                         gtgCGACGAAGATACCGCCAGGATTCAGGCAACGTTTGCAGCCTTCATAAAATGCCg                                        <  1:2202452/57‑1 (MQ=255)                                         gtgCGACGAAGATACCGCCAGGATTCAGGCAACGTTTGCAGCCTTCATAAAATGCCg                                        <  1:962405/57‑1 (MQ=255)                                         gtgCGACGAAGATACCGCCAGGATTCAGGCAACGTTTGCAGCCTTCATAAAATGCCg                                        <  1:1218082/57‑1 (MQ=255)                                         gtgCGACGAAGATACCGCCAGGATTCAGGCAACGTTTGCAGCCTTCATAAAATGCCg                                        <  1:1683587/57‑1 (MQ=255)                                         gtgCGACGAAGATACCGCCAGGATTCAGGCAACGTTTGCAGCCTTCATAAAATGCCGAAGTGAAAAGGCt                           <  1:3482632/70‑1 (MQ=255)                                              acgaAGATACCGCCAGGATTCAGGCAACGTTTGCAGCCTTCATAAAATGCCGAAGTGAAAAGGCTTTCGcc                     <  1:1429489/71‑1 (MQ=255)                                                 aaGATACCGCCAGGATTCAGGTAACGTTTGCAGCCTTCATAAAATGCCGa                                       <  1:3862322/50‑1 (MQ=255)                                                 aaGATACCGCCAGGATTCAGGTAACGTTTGCAGCCTTCATAAAATGCCGa                                       <  1:1773326/50‑1 (MQ=255)                                                   gATACCGCCAGGATTCAGGTAACGTTTGCAGCCTTCATaaaa                                             >  1:3435323/1‑42 (MQ=255)                                                   gATACCGCCAGGATTCAGGTAACGTTTGCAGCCTTCATAAAATGCCGaa                                      >  1:2363701/1‑49 (MQ=255)                                                   gATACCGCCAGGATTCAGGTAACGTTTGCAGCCTTCATAAAATGCCGAAg                                     >  1:1162752/1‑50 (MQ=255)                                                   gATACCGCCAGGATTCAGGCAACGTTTGCAGCCTTCATAAAATGCCGAAGTGAAAAGGCTTTCGCCg                    >  1:529120/1‑67 (MQ=255)                                                   gATACCGCCAGGATTCAGGCAACGTTTGCAGCCTTCATAAAATGCCGAAGTGAAAAGGCTTTCGCCg                    >  1:1351698/1‑67 (MQ=255)                                                     tACCGCCAGGATTCAGGCAACGTTTGCAGCCTTCATaa                                               <  1:1126025/38‑1 (MQ=255)                                                     tACCGCCAGGATTCAGGCAACGTTTGCAGCCTTCATaa                                               <  1:4021280/38‑1 (MQ=255)                                                     tACCGCCAGGATTCAGGCAACGTTTGCAGCCTTCATaa                                               <  1:3335243/38‑1 (MQ=255)                                                     tACCGCCAGGATTCAGGCAACGTTTGCAGCCTTCATaa                                               <  1:3977930/38‑1 (MQ=255)                                                     tACCGCCAGGATTCAGGCAACGTTTGCAGCCTTCATAAAATGCCGAAGTGAAAAGGCTTTCGCCGGGACCg              <  1:228816/71‑1 (MQ=255)                                                     tACCGCCAGGATTCAGGCAACGTTTGCAGCCTTCATAAAATGCCGAAGTGAAAAGGCTTTCGCCGGGACCg              <  1:3095069/71‑1 (MQ=255)                                                       ccGCCAGGATTCAGGTAACGTTTGCAGCCTTCATAAAATGCCGAAGTGAAAAGGCTTTCGCCGGGACCGa             <  1:1513234/70‑1 (MQ=255)                                                           cAGGATTCAGGCAACGTTTGCAGCCTTCATAAAATGCCGAAGTGAAAAGGCTTTCGCCGGGACCGATAGGa        >  1:4397965/1‑71 (MQ=255)                                                           cAGGATTCAGGCAACGTTTGCAGCCTTCATAAAATGCCGAAGTGAAAAGGCTTTCGCCGGGACCGATAGGa        >  1:3356452/1‑71 (MQ=255)                                                           cAGGATTCAGGCAACGTTTGCAGCCTTCATAAAATGCCGAAGTGAAAAGGCTTTCGCCGGGACCGATAGGa        >  1:461652/1‑71 (MQ=255)                                                            aGGATTCAGGCAACGTTTGCAGCCTTCATAAAATGCCGAAGTGa                                  <  1:4223371/44‑1 (MQ=255)                                                              gATTCAGGCAACGTTTGCAGCCTTCATAAAATGCCGAAGTTAAAAGGCTTTCGCCGGGACCGa             <  1:975932/63‑1 (MQ=255)                                                              gATTCAGGCAACGTTTGCAGCCTTCATAAAATGCCGAAGTGAAAAGGCTTTc                        <  1:460105/52‑1 (MQ=255)                                                              gATTCAGGCAACGTTTGCAGCCTTCATAAAATGCCGAAGTGAAAAGGCTTTc                        <  1:3179154/52‑1 (MQ=255)                                                              gATTCAGGCAACGTTTGCAGCCTTCATAAAATGCCGAAGTGAAAAGGCTTTc                        <  1:1668892/52‑1 (MQ=255)                                                               aTTCAGGTAACGTTTGCAGCCTTCATAAAATGCCGAAGTGAAAAGGCTTTCGCCGGGACCGATAGGATCgg    <  1:3996563/71‑1 (MQ=255)                                                               aTTCAGGTAACGTTTGCAGCCTTCATAAAATGCCGAAGTGAAAAGGCTTTCGCCGGGACCGATAGGATCgg    <  1:2288642/71‑1 (MQ=255)                                                                ttCAGGTAACGTTTGCAGCCTTCATAAAATGCCGAAGTGAAAAGGCTTTCGCCGGGACCGATAGGATCgg    <  1:2569605/70‑1 (MQ=255)                                                                ttCAGGCAACGTTTGCAGCCTTCATAAAATGCCGAAGTGAAAAGGCTTTCGCCGGGACCGATAGGATCGGt   <  1:4249148/71‑1 (MQ=255)                                                                 tCAGGCAACGTTTGCAGCCTTCATAAAATGCCGAAGTGAAAAGGCTTTCGCCGGGACCGATAGGATCGGt   >  1:1592468/1‑70 (MQ=255)                                                                 tCAGGCAACGTTTGCAGCCTTCATAAAATGCCGAAGTGAAAAGGCTTTCGCCGGGACCGATAGGATCGGTg  >  1:2534461/1‑71 (MQ=255)                                                                  cAGGCAACGTTTGCAGCCTTCATAAAATGCCGAAGTGAAAAGGCTTTCGCCGGGACCGAt            >  1:2305083/1‑60 (MQ=255)                                                                      cAACGTTTGCAGCCTTCATAAAATGCCGAAGTGAAAAGGCTTTCGCCGGGACCTATAg          >  1:3231399/1‑58 (MQ=255)                                                                      cAACGTTTGCAGCCTTCATAAAATGCCGAAGTGAAAAGGCTTTCGCCGGGACCGATAg          >  1:4126654/1‑58 (MQ=255)                                                                      cAACGTTTGCAGCCTTCATAAAATGCCGAAGTGAAAAGGCTTTCGCCGGGACCGATAg          >  1:4095793/1‑58 (MQ=255)                                                                      |                                                                  TCGATGGCTTCTTCCTGCTGTAAAAAGCAGACGCCGTTTTGTGCGACGAAGATACCGCCAGGATTCAGGCAACGTTTGCAGCCTTCATAAAATGCCGAAGTGAAAAGGCTTTCGCCGGGACCGATAGGATCGGTG  >  minE/120594‑120728

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑