| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,093,658 | T→C | 20.8% | T907A (ACA→GCA) | ydiJ ← | predicted FAD‑linked oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,093,658 | 0 | T | C | 20.8% | 216.4 / 54.2 | 101 | T907A (ACA→GCA) | ydiJ | predicted FAD‑linked oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base T (28/52); new base C (14/7); total (42/59) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.25e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.78e-01 | |||||||||||
Only 100 of 105 total aligned reads displayed.
GCGCACCCGGCAAGGCGGTAACTTCGGTACAGTGACCAAAGAAATACCATGATTCACCGCTGACTGTAGCCACTGGCTGTGACTCAAGTGCGCTTGCCAGCCATTCATTCGCCAGTAAGACGTTAAACTCGCCACGC > minE/1093592‑1093728 | gcgcACCCGGCAAGGCGGTAACTTCGGTACAGTGACCAAAGAAATACCATGATTCACCGCTGACTGTAGc < 1:2897164/70‑1 (MQ=255)gcgcACCCGGCAAGGCGGTAACTTCGGTACAGTGACCAAAGAAATACCATGATTCACCGCTGACTGTAGc < 1:790626/70‑1 (MQ=255) cGGCAAGGCGGTAACTTCGGTACAGTGACCAAAGAAATACCATGATTCACCGCTGACTGCAGCCACTGGCt > 1:3538754/1‑71 (MQ=255) ggCAAGGCGGTAACTTCGGTACAGTGACCAAAGAAATACCATGATTCACCGCTGACTGTAGCCACTGGCtg > 1:3000481/1‑71 (MQ=255) ggCAAGGCGGTAACTTCGGTACAGTGACCAAAGAAATACCATGATTCACCGCTGACTGCAGCCACTGGCtg > 1:128765/1‑71 (MQ=255) ggCAAGGCGGTAACTTCGGTACAGTGACCAAAGAAATACCATGATTCACCGCTGACTGCAGCCACTGGCtg > 1:4748707/1‑71 (MQ=255) gCAAGGCGGTAACTTCGGTACAGTGACCAATGAAATACCATGATTCACCGCTGACTGTAGc < 1:1743769/61‑1 (MQ=255) gCAAGGCGGTAACTTCAGTACAGTGACCAAAGAAATACCATGATTCACCGCTGACTGTAGCCACTGGCtgt < 1:1072304/71‑1 (MQ=255) cAAGGCGGTAACTTCGGTACAGTGACCAAAGAAATACCATGATTCACCGCTGACTGTAGCCACTGGCtgtg < 1:3186342/71‑1 (MQ=255) gCGGTAACTTCGGTACAGTGACCAAAGAAATACCATGATTCACCGCTGACTGTAGCCACTGGCTGTGACt > 1:1553164/1‑70 (MQ=255) ggTAACTTCGGTACAGTGACCAAAGAAATACCATGATTCACCGCTGACTGTAGCCACTGGCTGTGACTCaa < 1:2185484/71‑1 (MQ=255) tAACTTCGGTACAGTGACCAAAGAAATACCATGATTCACCGCTGACTGTAGCCACTGGCTGTGAc < 1:3828780/65‑1 (MQ=255) tAACTTCGGTACAGTGACCAAAGAAATACCATGATTCACCGCTGACTGTAGCCACTGGCTGTGAc < 1:1976994/65‑1 (MQ=255) tAACTTCGGTACAGTGACCAAAGAAATACCATGATTCACCGCTGACTGTAGCCACTGGCTGTGACTCAAGt < 1:636766/71‑1 (MQ=255) tAACTTCGGTACAGTGACCAAAGAAATACCATGATTCACCGCTGACTGTAGCCACTGGCTGTGACTCAAGt < 1:221310/71‑1 (MQ=255) aCTTCGGTACAGTGACCAAAGAAATACCATGATTCACCGCTGACTGTAGCCACTgg < 1:1992777/56‑1 (MQ=255) aCTTCGGTACAGTGACCAAAGAAATACCATGATTCACCGCTGACTGTAGCCACTgg < 1:1431394/56‑1 (MQ=255) aCTTCGGTACAGTGACCAAAGAAATACCATGATTCACCGCTGACTGTAGCCACTgg < 1:3717770/56‑1 (MQ=255) cTTCGGTACAGTGACCAAAGAAATACCATGATTCACCGCTGACTGTAGCCACTGGCTGTGa < 1:823933/61‑1 (MQ=255) cTTCGGTACAGTGACCAAAGAAATACCATGATTCACCGCTGACTGTAGCCACTGGCTGTGACTCAAGTgcg < 1:1884416/71‑1 (MQ=255) cTTCGGTACAGTGACCAAAGAAATACCATGATTCACCGCTGACTGTAGCCACTGGCTGTGACTCAAGTgcg < 1:2778013/71‑1 (MQ=255) ttCGGTACAGTGACCAAAGAAATACCATGATTCACCGCTGACTGTAGcc < 1:144055/49‑1 (MQ=255) ttCGGTACAGTGACCAAAGAAATACCATGATTCACCGCTGACTGTAGcc < 1:4545353/49‑1 (MQ=255) ttCGGTACAGTGACCAAAGAAATACCATGATTCACCGCTGACTGTAGCCACTGGCTGTGACTCAAGTg < 1:2213710/68‑1 (MQ=255) ttCGGTACAGTGACCAAAGAAATACCATGATACACCGCTGACTGTAGcc < 1:3074607/49‑1 (MQ=255) tCGGTACAGTGACCAAAGAAATACCATGATTCACCGCTGACTGTAGCCACTGGCTGTGACTCAAGTGCGCt > 1:2262173/1‑71 (MQ=255) tCGGTACAGTGACCAAAGAAATACCATGATTCACCGCTGACTGCAGCCACTGGCTGTGACTCAAGTGCGCt > 1:3534134/1‑71 (MQ=255) tCGGTACAGTGACCAAAGAAATACCATGATTCACCGCTGACTGCAGCCACTGGCTGTGACTCAAGTGCGCt > 1:2735193/1‑71 (MQ=255) tCGGTACAGTGACCAAAGAAATACCATGATTCACCGCTGACTGCAGCCACTGGCTGTGACTCAAGTGCGCt > 1:2902779/1‑71 (MQ=255) tCGGTACAGTGACCAAAGAAATACCATGATTCACCGCTGACTGCAGCCACTGGCTGTGACTCAAGTGCGCt > 1:2082854/1‑71 (MQ=255) tCGGTACAGTGACCAAAGAAATACCATGATTCACCGCTGACTGCAGCCACTGGCTGTGACTCAAGTGCGCt > 1:3623312/1‑71 (MQ=255) cGGTACAGTGACCAAAGAAATACCATGATTCACCGCTGACTGTAGCCACTGGCTGTGa > 1:1380149/1‑58 (MQ=255) cGGTACAGTGACCAAAGAAATACCATGATTCACCGCTGACTGTAGCCACTGGCTGTGa > 1:32058/1‑58 (MQ=255) cGGTACAGTGACCAAAGAAATACCATGATTCACCGCTGACTGTAGCCACTGGCTGTGa > 1:4498860/1‑58 (MQ=255) cGGTACAGTGACCAAAGAAATACCATGATTCACCGCTGACTGTAGCCACTGGCTGTGa > 1:1829858/1‑58 (MQ=255) cGGTACAGTGACCAAAGAAATACCATGATTCACCGCTGACTGTAGCCACTGGCTGTGACTCAAGTGCGCtt > 1:3960713/1‑71 (MQ=255) cGGTACAGTGACCAAAGAAATACCATGATTCACCGCTGACTGTAGCCACTGGCTGTGACTCAAGTGCGCtt > 1:3761624/1‑71 (MQ=255) cGGTACAGTGACCAAAGAAATACCATGATTCACCGCTGACTGCAGCCa > 1:4362988/1‑48 (MQ=255) ggTACAGTGACCAAAGAAATACCATGATTCACCGCTGACTGTAGCCACTGGCTGTGACTCAAGTGCGCtt < 1:1949694/70‑1 (MQ=255) ggTACAGTGACCAAAGAAATACCATGATTCACCGCTGACTGTAGCCACTGGCTGTGACTCAAGTGCGCtt < 1:3238527/70‑1 (MQ=255) ggTACAGTGACCAAAGAAATACCATGATTCACCGCTGACTGTAGCCACTGGCTGTGACTCAAGTGCGCtt < 1:1146323/70‑1 (MQ=255) ggTACAGTGACCAAAGAAATACCATGATTCACCGCTGACTGTAGCCACTGGCTGTGACTCAAGTGCGCtt < 1:3744262/70‑1 (MQ=255) gTACAGTGACCAAAGAAATACCATGATTCACCGCTGACTGTAGCCACTGGCTGTGACTCAAGTGCGCTTgc < 1:344842/71‑1 (MQ=255) gTACAGTGACCAAAGAAATACCATGATTCACCGCTGACTGTAGCCACTGGCTGTGACTCAAGTGCGCTTgc < 1:3212462/71‑1 (MQ=255) gTACAGTGACCAAAGAAATACCATGATTCACCGCTGACTGTAGCCACTGGCTGTGACTCAAGTGCGCTTgc < 1:1299060/71‑1 (MQ=255) tACAGTGACCAAAGAAATACCATGATTCACCGCTGACTGTAGCCACTgg < 1:3494233/49‑1 (MQ=255) tACAGTGACCAAAGAAATACCATGATTCACCGCTGACTGTAGCCACTGGCTGTGACTCAAGTGCGCTTgc > 1:724850/1‑70 (MQ=255) cAGTGACCAAAGAAATACCATGATTCACCGCTGACTGTAGCCACTGGCTGTGACTCAAGTGCGCTTgccag < 1:2777627/71‑1 (MQ=255) cAGTGACCAAAGAAATACCATGATTCACCGCTGACTGTAGCCACTGGCTGTGACTCAAGTGCGCTTgccag > 1:679939/1‑71 (MQ=255) aGTGACCAAAGAAATACCATGATTCACCGCTGACTGTAGCCACTg < 1:337843/45‑1 (MQ=255) aGTGACCAAAGAAATACCATGATTCACCGCTGACTGTAGCCACTg < 1:4089544/45‑1 (MQ=255) aGTGACCAAAGAAATACCATGATTCACCGCTGACTGTAGCCACTg < 1:343404/45‑1 (MQ=255) aGTGACCAAAGAAATACCATGATTCACCGCTGACTGTAGCCACTg < 1:3133487/45‑1 (MQ=255) aGTGACCAAAGAAATACCATGATTCACCGCTGACTGTAGCCACTg < 1:1880429/45‑1 (MQ=255) aGTGACCAAAGAAATACCATGATTCACCGCTGACTGTAGCCACTg < 1:139777/45‑1 (MQ=255) gTGACCAAAGAAATACCATGATTCACCGCTGACTGTAGCCACTGGCTGTGACTCAAGTGCGCTTgccagcc < 1:3921864/71‑1 (MQ=255) gTGACCAAAGAAATACCATGATTCACCGCTGACTGTAGCCACTGGCTGTGACTCAAGTGCGCTTgccagc > 1:1553277/1‑70 (MQ=255) gTGACCAAAGAAATACCATGATTCACCGCTGACTGTAGCCACTGGCTGTGACTCAAGTGCGCTTgccag < 1:1327589/69‑1 (MQ=255) aCCAAAGAAATACCATGATTCACCGCTGACTGTAGCCACTGGCTGTGACTCAAGTGCGCTTGCCAGCcat < 1:3021206/70‑1 (MQ=255) aaGAAATACCATGATTCACCGCTGACTGCAGCCACTGGCTGTGACTc < 1:3749236/47‑1 (MQ=255) aGAAATACCATGATTCACCGCTGACTGTAGCCACTGGCTGTGACTCAAGTGCGCTTGCCAGCcattcatt > 1:1674905/1‑70 (MQ=255) aGAAATACCATGATTCACCGCTGACTGTAGCCACTGGCTGTGACTCAAGTGCGCTTGCCAGCCattcattc > 1:1680434/1‑71 (MQ=255) aGAAATACCATGATTCACCGCTGACTGCAGCCACTGGCTGTGa > 1:1851425/1‑43 (MQ=255) gAAATACCATGATTCACCGCTGACTGCAGCCACTGGCTGTGACTCaa > 1:1178087/1‑47 (MQ=255) gAAATACCATGATTCACCGCTGACTGCAGCCACTGGCTGTGACTCaa > 1:168247/1‑47 (MQ=255) gAAATACCATGATTCACCGCTGACTGCAGCCACTGGCTGTGACTCaa > 1:704892/1‑47 (MQ=255) gAAATAACATGATTCACCGCTGACTGCAGCCACTGGCTGTGACTCaa > 1:3345135/1‑47 (MQ=255) aaaTACCATGATTCACCGCTGACTGCAGCCACTGGCTGTGACTCAAGTGCGCTTGCCAGCCATTCATTCGc < 1:1153851/71‑1 (MQ=255) tACCATGATTCACCGCTGACTGTAGCCACTGGCtgt < 1:2713906/36‑1 (MQ=255) tACCATGATTCACCGCTGACTGTAGCCACTGGCtgt < 1:641328/36‑1 (MQ=255) aCCATGATTCACCGCTGACTGTAGCCACTGGCTGTGa > 1:3067551/1‑37 (MQ=255) aCCATGATTCACCGCTGACTGTAGCCACTGGCTGTGa > 1:2448420/1‑37 (MQ=255) ccATGATTCACCGCTGACTGTAGCCACTGGCTGTGa > 1:2636220/1‑36 (MQ=255) ccATGATTCACCGCTGACTGTAGCCACTGGCTGTGa > 1:1865833/1‑36 (MQ=255) ccATGATTCACCGCTGACTGTAGCCACTGGCTGTGACTCAAGTGCGCTTGCCAGCCATTCATTCGCCAGt < 1:2691435/70‑1 (MQ=255) ccATGATTCACCGCTGACTGTAGCCACTGGCTGTGACTCAAGTGCGCTTGCCAGCCATTCATTCGCCAGt < 1:2605840/70‑1 (MQ=255) ccATGATTCACCGCTGACTGTAGCCACTGGCTGTGACTCAAGTGCGCTTGCCAGCCATTCATTCGCCAGt < 1:1801392/70‑1 (MQ=255) cATGATTCACCGCTGACTGTAGCCACTGGCTGTGACTCAAGTGCGCTTgcca < 1:4074518/52‑1 (MQ=255) cATGATTCACCGCTGACTGTAGCCACTGGCTGTGACTCAAGTGCGCTTGCCAGCCATTCATTCGc < 1:995224/65‑1 (MQ=255) cATGATTCACCGCTGACTGTAGCCACTGGCTGTGACTCAAGTGCGCTTGCCAGCCATTCATTCGc < 1:1129351/65‑1 (MQ=255) cATGATTCACCGCTGACTGTAGCCACTGGCTGTGACTCAAGTGCGCTTGCCAGCCATTCATTCGCCAGTaa < 1:3082609/71‑1 (MQ=255) tGATTCACCGCTGACTGTAGCCACTGGCTGTGACTCAAGTGCGCTTgccagc > 1:1318113/1‑52 (MQ=255) tGATTCACCGCTGACTGTAGCCACTGGCTGTGACTCAAGTGCGCTTgccagc > 1:188333/1‑52 (MQ=255) tGATTCACCGCTGACTGTAGCCACTGGCTGTGACTCAAGTGCGCTTGCCAGCCATTCATTCGCCa > 1:3057211/1‑65 (MQ=255) tGATTCACCGCTGACTGTAGCCACTGGCTGTGACTCAAGTGCGCTTGCCAGCCATTCATTCGCCa > 1:1399097/1‑65 (MQ=255) tGATTCACCGCTGACTGTAGCCACTGGCTGTGACTCAAGTGCGCTTGCCAGCCATTCATTCGCCa > 1:1825663/1‑65 (MQ=255) tGATTCACCGCTGACTGTAGCCACTGGCTGTGACTCAAGTGCGCTTGCCAGCCATTCATTCGCCAGTAAGa < 1:3149163/71‑1 (MQ=255) tGATTCACCGCTGACTGTAGCCACTGGCTGTGACTCAAGTGCGCTTGCCAGCCATTCATTCGCCAGTAAGa < 1:913951/71‑1 (MQ=255) gATTCACCGCTGACTGTAGCCACTGGCTGTGACTCa < 1:1406862/36‑1 (MQ=255) ttCACCGCTGACTGTAGCCACTGGCTGTGACTCAAg > 1:4562962/1‑36 (MQ=255) aCCGCTGACTGTAGCCACTGGCTGTGACTCAAGTGCGCTTGCCAGCCATTCATTCGCCAGTAAGACGTTa < 1:2797073/70‑1 (MQ=255) aCCGCTGACTGCAGCCACTGGCTGTGACTCAAGTGCGCTTGCCAGCCATTCATTCGCCAGTAAGACGTTaa < 1:1772694/71‑1 (MQ=255) aCCGCTGACTGCAGCCACTGGCTGTGACTCAAGTGCGCTTGCCAGCCATTCATTCGCCAGTAAGACGTTaa < 1:835273/71‑1 (MQ=255) cTGACTGTAGCCACTGGCTGTGACTCAAGTGCGCTTGCCAGCCATTCATTCGCCAGTaa > 1:2708653/1‑59 (MQ=255) cTGACTGCAGCCACTGGCTGTGACTCAAGTGCGCTTGCCAGCCATTCATTCGCCAGTAAGACGTTAAACTc < 1:2032890/71‑1 (MQ=255) aCTGCAGCCACTGGCTGTGACTCAAGTGCGCTTGCCAGCCATTCATTCGCCAGTAAGACGTTAAACTCGc < 1:3241946/70‑1 (MQ=255) tAGCCACTGGCTGTGACTCAAGTGCGCTTGCCAGCCATTCATTCGCCAGTAAGACGTTAAACTCGCCa > 1:2587798/1‑68 (MQ=255) tAGCCACTGGCTGTGACTCAAGTGCGCTTGCCAGCCATTCATTCGCCAGTAAGACGTTAAACTCGCCa > 1:2894310/1‑68 (MQ=255) tAGCCACTGGCTGTGACTCAAGTGCGCTTGCCAGCCATTCATTCGCCAGTAAGACGTTAAACTCGCCACGc < 1:2096153/71‑1 (MQ=255) tAGCCACTGGCTGTGACTCAAGTGCGCTTGCCAGCCATTCATTCGCCAGTAAGACGTTAAACTCGCCACGc < 1:4457608/71‑1 (MQ=255) | GCGCACCCGGCAAGGCGGTAACTTCGGTACAGTGACCAAAGAAATACCATGATTCACCGCTGACTGTAGCCACTGGCTGTGACTCAAGTGCGCTTGCCAGCCATTCATTCGCCAGTAAGACGTTAAACTCGCCACGC > minE/1093592‑1093728 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |