Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,139,620 T→C 24.5% S332S (AGT→AGC)  ydjN → predicted transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,139,6200TC24.5% 185.7 / 67.8 102S332S (AGT→AGC) ydjNpredicted transporter
Reads supporting (aligned to +/- strand):  ref base T (34/43);  new base C (15/10);  total (49/53)
Fisher's exact test for biased strand distribution p-value = 2.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.46e-01

Only 100 of 114 total aligned reads displayed.

GTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCCTCTTTCGGTGCAACCATTGGTCAGAACGGCTGCGCCGGTTTGTATCCGGCAATGCTGGCGG  >  minE/1139550‑1139690
                                                                      |                                                                      
gTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGt                                                                        <  1:4097900/71‑1 (MQ=255)
gTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGt                                                                        <  1:2203579/71‑1 (MQ=255)
gTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGt                                                                        >  1:1294991/1‑71 (MQ=255)
gTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGt                                                                        <  1:2709136/71‑1 (MQ=255)
gTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGt                                                                        <  1:3846575/71‑1 (MQ=255)
   ctgcGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTc                                                                     <  1:3045924/71‑1 (MQ=255)
   ctgcGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTc                                                                     <  1:693786/71‑1 (MQ=255)
      cGTCTATCCCACTGAATGTGGAAGCACAATCGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTcg                                                                    <  1:3458851/69‑1 (MQ=255)
      cGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTcg                                                                    <  1:3636955/69‑1 (MQ=255)
        tCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGtt                                                                       <  1:751378/64‑1 (MQ=255)
        tCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGtt                                                                       <  1:3824413/64‑1 (MQ=255)
           aTCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCctc                                                             >  1:1116197/1‑71 (MQ=255)
           aTCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCctc                                                             >  1:3311026/1‑71 (MQ=255)
            tCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCctct                                                            >  1:1356379/1‑71 (MQ=255)
            tCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCctct                                                            >  1:1992876/1‑71 (MQ=255)
              ccACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCCTCttt                                                          <  1:3029606/71‑1 (MQ=255)
              ccACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGCTTCGCCGCct                                                              >  1:1655939/1‑67 (MQ=255)
              ccACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGCTTCGCCGCCTCttt                                                          >  1:705454/1‑71 (MQ=255)
              ccACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGCTTCGCCGCCTCttt                                                          >  1:3111224/1‑71 (MQ=255)
              ccACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGCTTCGCCGCCTCttt                                                          >  1:2762723/1‑71 (MQ=255)
              ccACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGCTTCGCCGCCTCttt                                                          >  1:1984755/1‑71 (MQ=255)
               cACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCCTCTTTc                                                         <  1:1644565/71‑1 (MQ=255)
                aCTGAATGTGGAAGCACAACCGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCCTCTTTCg                                                        >  1:2866979/1‑71 (MQ=255)
                aCTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCCTCTTTCg                                                        >  1:3375619/1‑71 (MQ=255)
                  tGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCCTCTTTCGGt                                                      >  1:2510370/1‑71 (MQ=255)
                   gAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCCTCTTTCGGt                                                      >  1:365390/1‑70 (MQ=255)
                   gAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCCTCTTTCGGTg                                                     >  1:1507461/1‑71 (MQ=255)
                   gAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCCTCTTTCGGTg                                                     >  1:252082/1‑71 (MQ=255)
                    aaTGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCCTCTTTCg                                                        >  1:3947999/1‑67 (MQ=255)
                    aaTGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCCTCTTTCGGTg                                                     >  1:1663204/1‑70 (MQ=255)
                    aaTGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCCTCTTTCGGTg                                                     >  1:1566853/1‑70 (MQ=255)
                    aaTGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCCTCTTTCGGTg                                                     >  1:1120366/1‑70 (MQ=255)
                    aaTGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGCTTCGCCGCCTCTTTCGGTGc                                                    <  1:1360564/71‑1 (MQ=255)
                       gtgGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCCTCTTTCGGTGCAAc                                                 >  1:780016/1‑71 (MQ=255)
                        tgGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCTCCAGTTTCGCCGCCTCTTTCGGTGCAAc                                                 <  1:2072206/70‑1 (MQ=255)
                        tgGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCCTCTTTCGGTGCAAc                                                 <  1:4630921/70‑1 (MQ=255)
                        tgGAAGCACAAACGCGTCGTCTGGGCGTTCATGAATCCATCGCCAGTTTCGCCGCCTCTTTCGGTGCAAc                                                 <  1:2702665/70‑1 (MQ=255)
                          gAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCCTCTTTCGGTGCAACCAt                                              <  1:3402155/71‑1 (MQ=255)
                          gAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCCTCTTTCGGTGCAACCAt                                              <  1:3480905/71‑1 (MQ=255)
                          gAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCCTCTTTCGGTGCAACCAt                                              <  1:3240621/71‑1 (MQ=255)
                          gAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCCGCTTTCGGTGCAACCAt                                              <  1:2295096/71‑1 (MQ=255)
                          gAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGCTTCGCCGCCTCTTTCGGTGCAACCAt                                              >  1:1467070/1‑71 (MQ=255)
                              cacaAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGctt                                                                      >  1:2377559/1‑43 (MQ=255)
                              cacaAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGctt                                                                      >  1:3937233/1‑43 (MQ=255)
                              cacaAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGctt                                                                      >  1:4598561/1‑43 (MQ=255)
                                 aaaCGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGCTTCGCCGCCTCTTTCGGTGCAACCATTg                                            >  1:1274646/1‑66 (MQ=255)
                                 aaaCGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGCTTCGACGCCTCTTTCGGTGCAACCATTGGt                                          >  1:248401/1‑68 (MQ=255)
                                  aaCGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCCTCttt                                                          <  1:1333161/51‑1 (MQ=255)
                                  aaCGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCCTCTTTCGGTGCAACCATTGGTCAg                                       >  1:741410/1‑70 (MQ=255)
                                  aaCGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCCTCTTTCGGTGCAACCATTGGTCAg                                       >  1:828459/1‑70 (MQ=255)
                                    cgcgTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCCTCTTTCGGTGCAACCATTGGTCAGAAc                                    <  1:4287540/71‑1 (MQ=255)
                                    cgcgTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCCTCTTTCGGTGCAACCATTGGTCAGAAc                                    <  1:279562/71‑1 (MQ=255)
                                    cgcgTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCCTCTTTCGGTGCAACCATTGGTCAGAAc                                    <  1:4293273/71‑1 (MQ=255)
                                      cgtcgtCTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCCTCTTTCGGTGCAACCATTGGTCAGAACgg                                  <  1:4446214/71‑1 (MQ=255)
                                      cgtcgtCTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCCTCTTTCGGTGCAACCATTGGTCAGAACgg                                  <  1:917804/71‑1 (MQ=255)
                                      cgtcgtCTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCCTCTTTCGGTGCAACCATTGGTCAGAACgg                                  >  1:3165142/1‑71 (MQ=255)
                                       gtcgtcTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCCTCTTTCGGTGCAACCATTGGTCAGAACgg                                  <  1:727902/70‑1 (MQ=255)
                                          gtcTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCCTCTTTCg                                                        >  1:2204774/1‑45 (MQ=255)
                                          gtcTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCCTCTTTCg                                                        >  1:4248074/1‑45 (MQ=255)
                                          gtcTGGGCGTTCCTGAATCCATCGCCAGCTTCGCCGCCTCTTTCGGTGCAACCATTGGTCAGAACGGCTgc                              >  1:1825441/1‑71 (MQ=255)
                                          gtcTGGGCGTTCCTGAATCCATCGCCAGCTTCGCCGCCTCTTTCGGTGCAACCATTGGTCAGAACGGCTgc                              >  1:877833/1‑71 (MQ=255)
                                          gtcTGGGCGTTCCTGAATCCATCGCCAGCTTCGCCGCCTCTTTCGGTGCAACCATTGGTCAGAACGGCTgc                              >  1:231551/1‑71 (MQ=255)
                                          gtcTGGGCGTTCCTGAATCCATCGCCAGCTTCGCCGCCTCTTTCGGTGCAACCATTGGTCAGAACGGCTgc                              >  1:376137/1‑71 (MQ=255)
                                               ggCGTTCCTGAATCCATCGCCAGTTTCGCCGCCTCTTTCGGTGCAACCATTGGTCAGAACGGCTGCGCCgg                         <  1:2129477/71‑1 (MQ=255)
                                                gCGTTCCTGAATCCATCGCCAGTTTCGCCGCCTCTTTCGGTGCAACCATTGGTCAGAACGGCTGCGCCGGt                        <  1:866822/71‑1 (MQ=255)
                                                gCGTTCCTGAATCCATCGCCAGTTTCGCCGCCTCTTTCGGTGCAACCATTGGTCAGAACGGCTGCGCCGGt                        <  1:2298245/71‑1 (MQ=255)
                                                gCGTTCCTGAATCCATCGCCAGTTTCGCCGCCTCTTTCGGTGCAACCATTGGTCAGAACGGCTGCGCCGGt                        <  1:2085892/71‑1 (MQ=255)
                                                 cGTTCCTGAATCCATCGCCAGCTTCGCCGCCTCTTTCGGTGCAACCATTGGTCAGAACGGCTGCGCCGGtt                       <  1:1194689/71‑1 (MQ=255)
                                                  gTTCCTGAATCCATCGCCAGTTTCGCCGCCTCTTTCGGTGCAACCATTGGTCAGAACGGCTGCGCCGGt                        <  1:2939856/69‑1 (MQ=255)
                                                  gTTCCTGAATCCATCGCCAGCTTCGCCGCCTCTTTCGGTGCAACCATTGGTCAGAACGGCTGCGCCgg                         <  1:3345174/68‑1 (MQ=255)
                                                   ttCCTGAATCCATCGCCAGTTTCGCCGCCTCTTTCGGTGCAACCATTGGTCAGAACGGCTGCGCCGGt                        <  1:3546134/68‑1 (MQ=255)
                                                   ttCCTGAATCCATCGCCAGTTTCGCCGCCTCTTTCGGTGCAACCATTGGTCAGAACGGCTGCGCCGGt                        <  1:3333599/68‑1 (MQ=255)
                                                   ttCCTGAATCCATCGCCAGTTTCGCCGCCTCTTTCGGTGCAACCATTGGTCAGAACGGCTGCGCCGGTTTg                     <  1:201745/71‑1 (MQ=255)
                                                   ttCCTGAATCCATCGCCAGTTTCGCCGCCTCTTTCGGTGCAACCATTGGTCAGAACGGCTGCGCCGGTTTg                     <  1:207668/71‑1 (MQ=255)
                                                     ccTGAATCCATCGCCAGCTTCGCCGCCTCTTTCGGTGCAACCATTGGTCAGAACGGCTGCGCCGGTTTGt                    <  1:4525774/70‑1 (MQ=255)
                                                     ccTGAATCCATCGCCAGCTTCGCCGCCTCTTTCGGTGCAACCATTGGTCAGAACGGCTGCGCCGGTTTGt                    <  1:320534/70‑1 (MQ=255)
                                                     ccTGAATCCATCGCCAGCTTCGCCGCCTCTTTCGGTGCAACCATTGGTCAGAACGGCTGCGCCGGTTTGt                    <  1:112714/70‑1 (MQ=255)
                                                     ccTGAATCCATCGCCAGCTTCGCCGCCTCTTTCGGTGCAACCATTGGTCAGAACGGCTGCGCCGGTTTGt                    <  1:872999/70‑1 (MQ=255)
                                                         aaTCCATCGCCAGTTTCGCCGCCTCTTTCGGTGCAACCATTGGTCAGAACGGCTGCGCCGGTTTGTATCCg               >  1:1698100/1‑71 (MQ=255)
                                                         aaTCCATCGCCAGTTTCGCCGCCTCTTTCGGTGCAACCATTGGTCAGAACGGCTGAGCCGGTTTGTATc                 <  1:1218454/69‑1 (MQ=255)
                                                         aaTCCATCGCCAGTTTCGCCGCCTCTTTCGGTGCAACCATTGGTCAGAACGGCTGAGCCGGTTTGTATc                 <  1:1213003/69‑1 (MQ=255)
                                                         aaTCCATCGCCAGCTTCGCCGCCTCTTTCGGTGCAACCATTGGTCAg                                       <  1:641481/47‑1 (MQ=255)
                                                         aaTCCATCGCCAGCTTCGCCGCCTCTTTCGGTGCAACCATTGGTCAg                                       <  1:3212679/47‑1 (MQ=255)
                                                           tCCATCGCCAGTTTCGCCGCCTCTTTCGGTGCAACCATTGGTCAGAACGGCTGCGCCGGTTTGTATCCgg              >  1:2260780/1‑70 (MQ=255)
                                                           tCCATCGCCAGTTTCGCCGCCTCTTTCGGTGCAACCATTGGTCAGAACGGCTGCGCCGGTTTGTATCCGGc             <  1:3016507/71‑1 (MQ=255)
                                                           tCCATCGCCAGTTTCGCCGCCTCTTTCGGTGCAACCATTGGTCAGAACGGCTGCGCCGGTTTGTATCCGGc             <  1:2968192/71‑1 (MQ=255)
                                                           tCCATCGCCAGTTTCGCCGCCTCTTTCGGTGCAACCATTGGTCAGAACGGCTGCGCCGGTTTGTATCCGGc             <  1:854096/71‑1 (MQ=255)
                                                             cATCGCCAGTTTCGCCGCCTCTTTCGGTGCAACCATTGGTCAGa                                      <  1:1667960/44‑1 (MQ=255)
                                                               tCGCCAGTTTCGCCGCCTCTTTCGGTGCAACCATTGGTCAGAACGGCTGCGcc                           <  1:4652776/53‑1 (MQ=255)
                                                               tCGCCAGTTTCGCCGCCTCTTTCGGTGCAACCATTGGTCAGAACGGCTGCGcc                           <  1:345840/53‑1 (MQ=255)
                                                                cGCCAGTTTCGCCGCCTCTTTCGGTGCAACCATTGGTCAGAACGGCTGCGCCGGTTTGTATCCGGCAATg         <  1:772414/70‑1 (MQ=255)
                                                                cGCCAGTTTCGCCGCCTCTTTCGGTGCAACCATTGGTCAGAACGGCTGCGCCGGTTTGTATCCGGCAATGc        >  1:3938518/1‑71 (MQ=255)
                                                                cGCCAGTTTCGCCGCCTCTTTCGGTGCAACCATTGGTCAGAACGGCTGCGCCGGTTTGTATCCGGCAATGc        >  1:794436/1‑71 (MQ=255)
                                                                 gCCAGTTTCGCCGCCTCTTTCGGTGCAACCATTGGTCAGAACGGCTGCGCCGGTTTGTATCCGGCAATGCt       <  1:2526189/71‑1 (MQ=255)
                                                                 gCCAGCTTCGCCGCCTCTTTCGGTGCAACCATTGGTCa                                        >  1:1160068/1‑38 (MQ=255)
                                                                 gCCAATTTCGCCGCCTCGTTCGGCGCCACCATCGGCCAGAATGGCTGCGCCGGCATCTATCCGGCGATGCt       >  1:1084386/1‑71 (MQ=255)
                                                                 gCCAATTTCGCCGCCTCGTTCGGCGCCACCATCGGCCAGAATGGCTGCGCCGGCATCTATCCGGCGATGCt       >  1:2913442/1‑71 (MQ=255)
                                                                   cAGTTTCGCCGCCTCTTTCGGTGCAACCATTGGTCAGAACGGCTGCGCCGGTTTGTATCCGGCAATGCTgg     >  1:2534950/1‑71 (MQ=255)
                                                                    aGTTTCGCCGCCTCTTTCGGTGCAACCATTGGTCAGAACGGCTGCGCCGGTTTGTATCCGGCAATGCTGGc    >  1:2526906/1‑71 (MQ=255)
                                                                      tttCGCCGCCTCTTTCGGTGCAACCATTGGTCAGAACGGCTGCGCCGGTTTGTATCCGGCAATGCTGGCgg  >  1:4725756/1‑71 (MQ=255)
                                                                      |                                                                      
GTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCCTCTTTCGGTGCAACCATTGGTCAGAACGGCTGCGCCGGTTTGTATCCGGCAATGCTGGCGG  >  minE/1139550‑1139690

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑