BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	1,192,955	T→G	5.5%	E236D (GAA→GAC) ‡	fadD ←	acyl‑CoA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,192,955	0	T	G	5.5%	256.1 / 9.4	91	E236D (GAA→GAC) ‡	fadD	acyl‑CoA synthetase
Reads supporting (aligned to +/- strand): ref base T (54/32); new base G (2/3); total (56/35)
Fisher's exact test for biased strand distribution p-value = 3.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.30e-01

Only 100 of 107 total aligned reads displayed.

CAGCGGCAGCGCCGTCACCACCAGCTCTTTGCCCGGATGCAACAGCGGACCATAGGTCGCGTTAACCTGTTCCAGGTTCGCCAGCATATTGCGGTGAGTCAGCATCGCGCCTTTCGCCACACCAGTGGTGCCGCCGGTG  >  minE/1192886‑1193024

cAGCGGCAGCGCCGTCACCACCAGCTCTTTGCCCGGATGCAACAGCGGACCATAGGTCGCGTTAACCTGtt                                                                      <  1:2924698/71‑1 (MQ=255)

cAGCGGCAGCGCCGTCACCACCAGCTCTTTGCCCGGATGCAACAGCGGACCATAGGTCGCGTTAACCTGtt                                                                      <  1:1207427/71‑1 (MQ=255)

cAGCGGCAGCGCCGTCAACACCAGCTCTTTGCCCGGATGCAACAGCGGACCATAGGTCGCGTTAACCTGtt                                                                      <  1:2537574/71‑1 (MQ=255)

 aGCGGCAGCGCCGTCACCACCAGCTCTTTGCCCGGATGCAACAGCGGACCATAGGTCGCGTTAAGCTGTTc                                                                     <  1:3888/71‑1 (MQ=255)

 aGCGGCAGCGCCGTCACCACCAGCTCTTTGCCCGGATGCAACAGCGGACCATAGGTCGCGTTAACCTGTTc                                                                     <  1:855119/71‑1 (MQ=255)

      cAGCGCCGTCACCACCAGCTCTTTGCCCGGATGCAACAGCGGACCATAGGTCGCGTTAACCTGTTCCAGGt                                                                <  1:2188538/71‑1 (MQ=255)

      cAGCGCCGTCACCACAAGCTCTTTGCCCGGATGCAACAGCGGACCATAGGTCGCGTTAACCTGTTCCAGGt                                                                <  1:1662603/71‑1 (MQ=255)

          gcCGTCACCACCAGCTCTTTGCCCGGATGCAACAGCGGACCATAGGTCGCGTTAACCTGTTCCAGGTTCGc                                                            >  1:1492076/1‑71 (MQ=255)

              tCACCACCAGCTCTTTGCCCGGATGCAACAGCGGACCATAGGTCGCGTTAACCTGTTCCAGGt                                                                <  1:3747609/63‑1 (MQ=255)

              tCACCACCAGCTCTTTGCCCGGATGCAACAGCGGACCATAGGTCGCGTTAACCTGTTCCAGGt                                                                <  1:3555058/63‑1 (MQ=255)

              tCACCACCAGCTCTTTGCCCGGATGCAACAGCGGACCATAGGTCGCGTTAACCTGTTCCAGGt                                                                <  1:4258495/63‑1 (MQ=255)

              tCACCACCAGCTCTTTGCCCGGATGCAACAGCGGACCATAGGTCGCGTTAACCTGTTCCAGGt                                                                <  1:4327999/63‑1 (MQ=255)

              tCACCACCAGCTCTTTGCCCGGATGCAACAGCGGACCATAGGTCGCGTTAACCTGTTCCAGGt                                                                <  1:4478345/63‑1 (MQ=255)

              tCACCACCAGCTCTTTGCCCGGATGCAACAGCGGACCATAGGTCGCGTTAACCTGTTCCAGGt                                                                <  1:175731/63‑1 (MQ=255)

                accaccAGCTCTTTGCCCGGATGCAACAGCGGACCATAGGTCGCGTTAACCTGTTCCAGGTTCGCCAGc                                                        >  1:1295076/1‑69 (MQ=255)

                accaccAGCTCTTTGCCCGGATGCAACAGCGGACCATAGGTCGCGTTAACCTGTTCCAGGTTCGCCAGCa                                                       >  1:3825284/1‑70 (MQ=255)

                     caGCTCTTTGCCCGGATGCAACAGCGGACCATAGGTCGCGTTAACCTGTTCCAGGTTCGCCAGCATATTGc                                                 <  1:3429504/71‑1 (MQ=255)

                      aGCTCTTTGCCCGGATGCAACAGCGGACCATAGGTCGCGTTAACCTGTTCCAGGTTCGCCAGCATATTGCg                                                >  1:1869578/1‑71 (MQ=255)

                      aGCTCTTTGCCCGGATGCAACAGCGGACCATAGGTCGCGTTAACCTGTTCCAGGTTCGCCAGCATATTGCg                                                >  1:1019235/1‑71 (MQ=255)

                      aGCTCTTTGCCCGGATGCAACAGCGGACCATAGGTCGCGTTAACCTGTTCCAGGTTCGCCAGCATATTGCg                                                <  1:805560/71‑1 (MQ=255)

                          ctTTGCCCGGATGCAACAGCGGACCATAGGTCGCGTTAACCTGTTCCAGGTTCGCCAGCATATTGCGGt                                              >  1:2418748/1‑69 (MQ=255)

                              gCCCGGATGCAACAGCGGACCATAGGTCGCGTTAACCTGTTCCAGGTTCGCCAGCATATTGCGGTGAGTc                                         <  1:4198490/70‑1 (MQ=255)

                               cccGGATGCAACAGCGGACCATAGGTCGCGTTAACCTGTTCCAGGTTCGCCAGCATATTGCGGTGAGTCAg                                       <  1:3786130/71‑1 (MQ=255)

                                ccGGATGCAACAGCGGACCATAGGTCGCGTTAACCTGTTCCAGGTTCGCCAGCATATTGCGGTGAGTCAg                                       >  1:187732/1‑70 (MQ=255)

                                ccGGATGCAACAGCGGACCATAGGTCGCGTTAACCTGTTCCAGGTTCGCCAGCATATTGCGGTGAGTCAg                                       >  1:4374066/1‑70 (MQ=255)

                                ccGGATGCAACAGCGGACCATAGGTCGCGTTAACCTGTTCCAGGTTCGCCAGCATATTGCGGTGAGTCAGc                                      <  1:4181183/71‑1 (MQ=255)

                                  ggATGCAACAGCGGACCATAGGTCGCGTTAACCTGTTCCAGGTTCGCCAGCATATTGCGGTGAGTCa                                        >  1:608332/1‑67 (MQ=255)

                                  ggATGCAACAGCGGACCATAGGTCGCGTTAACCTGTTCCAGGTTCGCCAGCATATTGCGGTGAGTCAGCa                                     >  1:2724176/1‑70 (MQ=255)

                                  ggATGCAACAGCGGACCATAGGTCGCGTTAACCTGTTCCAGGTTCGCCAGCATATTGCGGTGAGTCAGCAt                                    >  1:1076830/1‑71 (MQ=255)

                                  ggATGCAACAGCGGACCATAGGTCGCGTTAACCTGTTCCAGGTTCGCCAGCATATTGCGGTGAGTCAGCAt                                    >  1:1071272/1‑71 (MQ=255)

                                  ggATGCAACAGCGGACCATAGGTCGCGTTAACCTGTTCCAGGTTCGCCAGCATATTGCGGTGAGTCAGCAt                                    >  1:3461390/1‑71 (MQ=255)

                                  ggATGCAACAGCGGACCATAGGTCGCGTTAACCTGTTCCAGGTTCGCCAGCATATTGCGGTGAGTCAGCAt                                    >  1:1935887/1‑71 (MQ=255)

                                  ggATGCAACAGCGGACCATAGGTCGCGTTAACCTGTTCCAGGTTCGCCAGCATATTGCGGTGAGTCAGCAt                                    >  1:1890982/1‑71 (MQ=255)

                                  ggATGCAACAGCGGACCATAGGTCGCGTTAACCTGTTCCAGGTTCGCCAGCATATTGCGGTGAGTCAGCAt                                    >  1:4732288/1‑71 (MQ=255)

                                  ggATGCAACAGCGGACCATAGGTCGCGTTAACCTGGAACAGGtt                                                               <  1:3040264/44‑1 (MQ=37)

                                  ggATGCAACAGCGGACCATAGGTCGCGTTAACCTGGAACAGGtt                                                               <  1:3029299/44‑1 (MQ=37)

                                  ggATGCAACAGCGGACCATAGGTCGCGTTAACCTGGAACAGGtt                                                               <  1:815886/44‑1 (MQ=37)

                                    aTGCAACAGCGGACCATAGGTCGCGTTAACCTGGAACAGGttaa                                                             >  1:4523749/1‑42 (MQ=25)

                                    aTGCAACAGCGGACCATAGGTCGCGTTAACCTGGAACAGGtta                                                              >  1:3933658/1‑42 (MQ=25)

                                          cAGCGGACCATAGGTCGCGTTAACCTGTTCCAGGTTCGCCAGCATATTGCGGTGAGTCAGCATCGCGCCtt                            >  1:3522283/1‑71 (MQ=255)

                                          cAGCGGACCATAGGTCGCGTTAACCTGTTCCAGGTTCGCCAGCATATTGCGGTGAGTCAGCATCGCGCCtt                            <  1:2416389/71‑1 (MQ=255)

                                          cAGCGGACCATAGGTCGCGTTAACCTGTTCCAGGTTCGCCAGCATATTGCGGTGAGTCAGCATCGCGCCtt                            >  1:4445477/1‑71 (MQ=255)

                                           aGCGGACCATAGGTCGCGTTAACCTGTTCCAGGTTCGCCAGCATATTGCGGTGAGTCAGCATCGCGCCttt                           >  1:3131587/1‑71 (MQ=255)

                                           aGCGGACCATAGGTCGCGTTAACCTGTTCCAGGTTCGCCAGCATATTGCGGTGAGTCAGCATCGCGCCttt                           <  1:3316572/71‑1 (MQ=255)

                                               taCCATAGGGCGCGTTAACCTGTTCCAGGTTCGCCAGCATATTGc                                                 <  1:1426392/44‑1 (MQ=255)

                                               gACCATAGGTCGCGTTAACCTGTTCCAGGTTCGCCAGCATATTGCGGTGAGTCAGCATCGCGCCTTTCGc                        >  1:2202702/1‑70 (MQ=255)

                                                aCCATAGGTCGCGTTAACCTGTTCCAGGTTCGCCAGCATATTGCGGTGAGTCAGCATCGCGCCTTTCGCca                      <  1:2033223/71‑1 (MQ=255)

                                                  cATAGGTCGCGTTAACCTGTTCCAGGTTCGCCAGCATATTGCGGTGAGTCAGCATCGCGCCTTTCGCcaca                    >  1:1483034/1‑71 (MQ=255)

                                                   aTAGGTCGCGTTAACCTGTTCCAGGTTCGCCAGCATATTGCGGTGAGTCAGCATcgcg                                >  1:3904691/1‑58 (MQ=255)

                                                     aGGTCGCGTTAACCTGTTCCAGGTTCGCCAGCATATTGCGGTGAGTGAGCATCGCGCCTTTCGCca                      >  1:836415/1‑66 (MQ=255)

                                                     aGGTCGCGTTAACCTGTTCCAGGTTCGCCAGCATATTGCGGTGAGTCAGCATCGCGCCTTTCGCCacac                   >  1:3868606/1‑69 (MQ=255)

                                                      ggTCGCGTTAACCTGTTCCAGGTTCGCCAGCATATTGCGGTGAGTCAGCATCGCGCCTTTc                          >  1:2500733/1‑61 (MQ=255)

                                                      ggTCGCGTTAACCTGTTCCAGGTTCGCCAGCATATTGCGGTGAGTCAGCATCGCGCCTTTCGCCACACCa                 <  1:75525/70‑1 (MQ=255)

                                                      ggTCGCGTTAACCTGTTCCAGGTTCGCCAGCATATTGCGGTGAGTCAGCATCGCGCCTTTCGCCACACCa                 <  1:3738930/70‑1 (MQ=255)

                                                      ggTCGCGTTAACCTGTTCCAGGTTCGCCAGCATATTGCGGTGAGTCAGCATCGCGCCTTTCGCCACACCAg                <  1:132544/71‑1 (MQ=255)

                                                       gTCGCGTTAACCTGTTCCAGGTTCGCCAGCATATTGCGGTGAGTCAGCATCGCGCCTTTCGc                        <  1:96497/62‑1 (MQ=255)

                                                       gTCGCGTTAACCTGTTCCAGGTTCGCCAGCATATTGCGGTGAGTCAGCATCGCGCCTTTCGc                        >  1:1855349/1‑62 (MQ=255)

                                                       gTCGCGTTAACCTGTTCCAGGTTCGCCAGCATATTGCGGTGAGTCAGCATCGCGCCTTTCGCCACAcc                  >  1:1925411/1‑68 (MQ=255)

                                                       gTCGCGTTAACCTGTTCCAGGTTCGCCAGCATATTGCGGTGAGTCAGCATCGCGCCTTTCGCCACACCa                 >  1:245165/1‑69 (MQ=255)

                                                       gTCGCGTTAACCTGTTCCAGGTTCGCCAGCATATTGCGGTGAGTCAGCATCGCGCCTTTCGCCACACCa                 >  1:1413002/1‑69 (MQ=255)

                                                       gTCGCGTTAACCTGTTCCAGGTTCGCCAGCATATTGCGGTGAGTCAGCATCGCGCCTTTCGCCACACCa                 >  1:1537115/1‑69 (MQ=255)

                                                       gTCGCGTTAACCTGTTCCAGGTTCGCCAGCATATTGCGGTGAGTCAGCATCGCGCCTTTCGCCACACCAgt               <  1:3540858/71‑1 (MQ=255)

                                                       gTCGCGTTAACCTGTTCCAGGTTCGCCAGCATATTGCGGTGAGTCAGCATCGCGCCTTTCGCCACACCAgt               <  1:2945651/71‑1 (MQ=255)

                                                         cgcgTTAACCTGTTCCAGGTTCGCCAGCATATTGCGGTGAGTCAGCATCGCGCCTTTCGCCACACCAgtgg             >  1:1652561/1‑71 (MQ=255)

                                                             ttAACCTGTTCCAGGTTCGCCAGCATATTGCGGTGAGt                                          <  1:3493701/38‑1 (MQ=255)

                                                             ttAACCTGTTCCAGGTTCGCCAGCATATTGCGGTGAGt                                          <  1:3567375/38‑1 (MQ=255)

                                                             ttAACCTGTTCCAGGTTCGCCAGCATATTGCGGTGAGTCAGCATcgcg                                <  1:1214562/48‑1 (MQ=255)

                                                             ttAACCTGTTCCAGGTTCGCCAGCATATTGCGGTGAGTCAGCATcgcg                                <  1:158283/48‑1 (MQ=255)

                                                             ttAACCTGTTCCAGGTTCGCCAGCATATTGCGGTGAGTCAGCATCGCGCCTTTCGCCACACCAGTGGTgcc         >  1:4691321/1‑71 (MQ=255)

                                                              tAACCTGTTCCAGGTTCGCCAGCATATTGCGGTGAGTCAGCATCGCGCCTTTCGCCACACCAGTGGTgccg        >  1:4615666/1‑71 (MQ=255)

                                                              tAACCTGTTCCAGGTTCGCCAGCATATTGCGGTGAGTCAGCATCGCGCCTTTCGCCACACCAGTGGTgccg        >  1:3189745/1‑71 (MQ=255)

                                                               aaCCTGTTCCAGGTTCGCCAGCATATTGCGGTGAGt                                          <  1:2361922/36‑1 (MQ=255)

                                                               aaCCTGTTCCAGGTTCGCCAGCATATTGCGGTGAGTCAGCATcgcg                                >  1:594774/1‑46 (MQ=255)

                                                               aaCCTGTTCCAGGTTCGCCAGCATATTGCGGTGAGTCAGCATCgcgc                               >  1:795793/1‑47 (MQ=255)

                                                               aaCCTGTTCCAGGTTCGCCAGCATATTGCGGTGAGTCAGCATCgcgc                               >  1:122993/1‑47 (MQ=255)

                                                               aaCCTGTTCCAGGTTCGCCAGCATATTGCGGTGAGTCAGCATCGCGCCttt                           >  1:4082748/1‑51 (MQ=255)

                                                               aaCCTGTTCCAGGTTCGCCAGCATATTGCGGTGAGTCAGCATCGCGCCttt                           >  1:4240735/1‑51 (MQ=255)

                                                               aaCCTGTTCCAGGTTCGCCAGCATATTGCGGTGAGTCAGCATCGCGCCttt                           >  1:308738/1‑51 (MQ=255)

                                                               aaCCTGTTCCAGGTTCGCCAGCATATTGCGGTGAGTCAGCATCGCGCCttt                           >  1:3286205/1‑51 (MQ=255)

                                                               aaCCTGTTCCAGGTTCGCCAGCATATTGCGGTGAGTCAGCATCGCGCCTTTCGCca                      >  1:3734040/1‑56 (MQ=255)

                                                               aaCCTGTTCCAGGTTCGCCAGCATATTGCGGTGAGTCAGCATCGCGCCTTTCGCca                      >  1:3887253/1‑56 (MQ=255)

                                                               aaCCTGTTCCAGGTTCGCCAGCATATTGCGGTGAGTCAGCATCGCGCCTTTCGCCACACCAGTGGTgccgc       >  1:347728/1‑71 (MQ=255)

                                                               aaCCTGTTCCAGGTTCGCCAGCATATTGCGGTGAGTCAGCATCGCGCCTTTCGCCACACCAGTGGTgccgc       >  1:3169981/1‑71 (MQ=255)

                                                               aaCCTGTTCCAGGTTCGCCAGCATATTGCGGTGAGTCAGCATCGCGCCTTTCGCCACACCAGTGGTgccgc       >  1:4124970/1‑71 (MQ=255)

                                                                   tGTTCCAGGTTCGCCAGCATATTGCGGTGAGTCAGCATCGCGCCTTTCGCCACACCAgtggt            >  1:2038107/1‑62 (MQ=255)

                                                                   tGTTCCAGGTTCGCCAGCATATTGCGGTGAGTCAGCATCGCGCCTTTCGCCACACCAgtggt            >  1:1036658/1‑62 (MQ=255)

                                                                    gTTCCAGGTTCGCCAGCATATTGCGGTGAGTCAGCATCGCGCCTTTCGCCACACCAGTGGTGCCGCCgg    >  1:146886/1‑69 (MQ=255)

                                                                    gTTCCAGGTTCGCCAGCATATTGCGGTGAGTCAGCATCGCGCCTTTCGCCACACCAGTGGTGCCGCCGgtg  >  1:2748148/1‑71 (MQ=255)

                                                                    gTTCCAGGTTCGCCAGCATATTGCGGTGAGTCAGCATCGCGCCTTTCGCCACACCAGTGGTGCCGCCGgtg  >  1:759755/1‑71 (MQ=255)

                                                                     ttCCAGGTTCGCCAGCATATTGCGGTGAGTCAGCATCGCGCCTTTCGCCACACCAgtg              <  1:2259047/58‑1 (MQ=255)

                                                                     ttCCAGGTTCGCCAGCATATTGCGGTGAGTCAGCATCGCGCCTTTCGCCACACCAgtg              <  1:3822139/58‑1 (MQ=255)

                                                                     ttCCAGGTTCGCCAGCATATTGCGGTGAGTCAGCATCGCGCCTTTCGCCACACCAgtg              <  1:3408085/58‑1 (MQ=255)

                                                                     ttCCAGGTTCGCCAGCATATTGCGGTGAGTCAGCATCGCGCCTTTCGCCACACCAGTGGTGCCGCCGgtg  <  1:4161385/70‑1 (MQ=255)

                                                                     ttCCAGGTTCGCCAGCATATTGCGGTGAGTCAGCATCGCGCCTTTCGCCACACCAGTGGTGCCGCCGgtg  <  1:2071296/70‑1 (MQ=255)

                                                                     ttCCAGGTTCGCCAGCATATTGCGGTGAGTCAGCATCGCGCCTTTCGCCACACCAGTGGTGCCGCCGgtg  <  1:651672/70‑1 (MQ=255)

                                                                     ttCCAGGTTCGCCAGCATATTGCGGTGAGTCAGCATCGCGCCTTTCGCCACACCAGTGGTGCCGCCGgtg  <  1:3375931/70‑1 (MQ=255)

                                                                     ttCCAGGTTCGCCAGCATATTGCGGTGAGTCAGCATCGCGCCTTTCGCCACACCAGTGGTGCCGCCGgtg  <  1:3718622/70‑1 (MQ=255)

                                                                     ttCCAGGTTCGCCAGCATATTGCGGTGAGTCAGCATCGCGCCTTTCGCCACACCAGTGGTGCCGCCGgtg  <  1:1062095/70‑1 (MQ=255)

                                                                     ttCCAGGTTCGCCAGCATATTGCGGTGAGTCAGCATCGCGCCTTTCGCCACACCAGTGGTGCCGCCGgtg  <  1:4437578/70‑1 (MQ=255)

                                                                     ttCCAGGTTCGCCAGCATATTGCGGTGAGTCAGCATCGCGCCTTTCGCCACACAAGTGGTGCCGCCGgtg  <  1:2315041/70‑1 (MQ=255)

CAGCGGCAGCGCCGTCACCACCAGCTCTTTGCCCGGATGCAACAGCGGACCATAGGTCGCGTTAACCTGTTCCAGGTTCGCCAGCATATTGCGGTGAGTCAGCATCGCGCCTTTCGCCACACCAGTGGTGCCGCCGGTG  >  minE/1192886‑1193024

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`