| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,225,925 | T→C | 8.6% | L77P (CTT→CCT) | yebV → | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,225,925 | 0 | T | C | 8.6% | 236.6 / 11.0 | 81 | L77P (CTT→CCT) | yebV | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (36/38); new base C (4/3); total (40/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.12e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTAAATGGCGAACACTCTGTCCTTTACCGTACCCGTTACGATCAACAATCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCGGCATGGCGGGTTATTTGTCCTGGTTATTCCCCCGTTGTAAAAT > minE/1225855‑1225993 | cTAAATGGCGAACACTCTGTCCTTTACCGTACCCGTTACGATCAACAATCTGATGCCTGGATTATGCGTCt > 1:4681680/1‑71 (MQ=255) aaaTGGCGAACACTCTGTCCTTTACCGTACCCGTTACGATCAACAATCTGATGCCTGGATTATGCGTCTTg > 1:350664/1‑71 (MQ=255) aaaTGGCGAACACTCTGTCCTTTACCGTACCCGTTACGATCAACAATCTGATGCCTGGATTATGCGTCTTg > 1:1726743/1‑71 (MQ=255) aaTGGCGAACACTCTGTCCTTTACCGTACCCGTTACGATCAACAATCTGATGCCTGGATTATGCGTCtt < 1:4217927/69‑1 (MQ=255) tGGCGAACACTCTGTCCTTTACCGTACCCGTTACGATCAACAATCTGATGCCTGGATTATGCGTCCTGCCt < 1:3712900/71‑1 (MQ=255) aaCACTCTGTCCTTTACCGTACCCGTTACGATCAACAATCTGATGCCTGGATTATGCGTCCTGCCTGATc > 1:2770184/1‑70 (MQ=255) aaCACTCTGTCCTTTACCGTACCCGTTACGATCAACAATCTGATGCCTGGATTATGCGTCCTGCCTGATc > 1:2650761/1‑70 (MQ=255) acacTCTGTCCTTTACCGTACCCGTTACGATCAACAATCTGATGCCTGGATTATGCGTCTTGCCTGATc > 1:1090737/1‑69 (MQ=255) cacTCTGTCCTTTACCGTACCCGTTACGATCAACAATCTGATGCCTGGATTATGCGTCCTGCCTGATCCaa < 1:4340774/71‑1 (MQ=255) tCCTTTACCGTACCCGTTACGATCAACAATCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAg < 1:4314006/68‑1 (MQ=255) tCCTTTACCGTACCCGTTACGATCAACAATCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAg < 1:3220306/68‑1 (MQ=255) ccTTTACCGTACCCGTTACGATCAACAATCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAAcc > 1:893098/1‑71 (MQ=255) ttACCGTACCCGTTACGATCAACAATCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGt > 1:23857/1‑71 (MQ=255) ttACCGTACCCGTTACGATCAACAATCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGt > 1:1978566/1‑71 (MQ=255) tACCGTACCCGTTACGATCAACAATCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCg < 1:2828007/69‑1 (MQ=255) tACCGTACCCGTTACGATCAACAATCTGATGCCTGGATTATGCGTCCTGCCTGATCCAAAAAGAACCCg < 1:3013384/69‑1 (MQ=255) aCCGTACCCGTTACGATCAACAATCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTc < 1:2427829/70‑1 (MQ=255) aCCGTACCCGTTACGATCAACAATCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTc < 1:1768942/70‑1 (MQ=255) aCCGTACCCGTTACGATCAACAATCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTc < 1:4623815/70‑1 (MQ=255) cGTACCCGTTACGATCAACAATCTGATGCCTGGATTATGCGTCTTGCCTGATCCaaa < 1:3155362/57‑1 (MQ=255) cGTACCCGTTACGATCAACAATCTGATGCCTGGATTATGCGTCTTGCCTGATCCaaa < 1:2126508/57‑1 (MQ=255) cGTACCCGTTACGATCAACAATCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCgg > 1:4183816/1‑70 (MQ=255) cGTACCCGTTACGATCAACAATCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCgg > 1:2784084/1‑70 (MQ=255) gTTACGATCAACAATCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAAc > 1:2503029/1‑56 (MQ=255) ttACGATCAACAATCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCgg < 1:4246453/62‑1 (MQ=255) ttACGATCAACAATCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCgg < 1:883338/62‑1 (MQ=255) ttACGATCAACAATCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCgg < 1:4135382/62‑1 (MQ=255) tACGATCAACAATCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGaa > 1:1859110/1‑53 (MQ=255) tACGATCAACAATCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGaa > 1:1583611/1‑53 (MQ=255) tACGATCAACAATCTGATGCCTGGATTATGCGTCCTGCCTGATCCAAAAAGAACCCGTCGGCATGGCGGGt > 1:4271455/1‑71 (MQ=255) tACGATCAACAATCTGATGCCTGGATTATGCGTCCTGCCTGATCCAAAAAGAACCCGTCGGCATGGCGGGt > 1:4162887/1‑71 (MQ=255) aCGATCAACAATCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCGGCa > 1:154467/1‑62 (MQ=255) aCGATCAACAATCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCGGCa > 1:736866/1‑62 (MQ=255) aCGATCAACAATCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCGGCa > 1:2088951/1‑62 (MQ=255) aCGATCAACAATCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCGGCa > 1:1725813/1‑62 (MQ=255) aCGATCAACAATCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCGGCATGGCGGGt > 1:1164711/1‑70 (MQ=255) aCGATCAACAATCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCGGCATGGCGGGt > 1:1367280/1‑70 (MQ=255) aCGATCAACAATCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCGGCATGGCGGGt > 1:2953383/1‑70 (MQ=255) cGATCAACAATCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCGGCATGGCGGGTTa < 1:2153486/71‑1 (MQ=255) cGATCAACAATCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCGGCATGGCGGGTTa < 1:1357825/71‑1 (MQ=255) cGATCAACAATCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCGGCATGGCGGGTTa < 1:4108847/71‑1 (MQ=255) gATCAAGAATCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCGGCATGGCGGGTTAt < 1:493320/71‑1 (MQ=255) gATCAACAATCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCGGCATGGCGGGTTAt < 1:3786325/71‑1 (MQ=255) gATCAACAATCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCGGCATGGCGGGTTAt < 1:169019/71‑1 (MQ=255) gATCAACAATCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCGGCATGGCGGGTTAt < 1:3441284/71‑1 (MQ=255) tCAACAATCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCGGCa > 1:1748508/1‑58 (MQ=255) tCAACAATCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCGGCATGGCGGGTTAttt > 1:113249/1‑71 (MQ=255) tCAACAATCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCGGCATGGCGGGTTAttt > 1:752616/1‑71 (MQ=255) caacaaTCTGATGCCTGGATTATGCGTCTTGCCTGATCCaaa < 1:4022239/42‑1 (MQ=255) caacaaTCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCGGCATGGCGGGTTATTTg > 1:4633650/1‑71 (MQ=255) caacaaTCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCGGCATGGCGGGTTATTTg > 1:3719460/1‑71 (MQ=255) aacaaTCTGATGCCTGGATTATGCGTCTTGCCTGATCCaaa < 1:3589597/41‑1 (MQ=255) aacaaTCTGATGCCTGGATTATGCGTCTTGCCTGATCCaaa < 1:4146275/41‑1 (MQ=255) aacaaTCTGATGCCTGGATTATGCGTCTTGCCTGATCCaaa < 1:3094818/41‑1 (MQ=255) aacaaTCTGATGCCTGGATTATGCGTCTTGCCTGATCCaaa < 1:814675/41‑1 (MQ=255) aacaaTCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAAc > 1:278059/1‑47 (MQ=255) acaaTCTGATGCCTGGATTATGCGTCTTGCCTGATCCaaa < 1:2088513/40‑1 (MQ=255) acaaTCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCGGCATGGCGGGTTATTTGTc < 1:1563236/71‑1 (MQ=255) caaTCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCGGCATGGCGGGTTATTTGTc < 1:2666349/70‑1 (MQ=255) aTCTGATGCCTGGATTATGCGTCTTGCCTGATCCaaa < 1:2855339/37‑1 (MQ=255) aTCTGATGCCTGGATTATGCGTCTTGCCTGATCCaaa < 1:4377854/37‑1 (MQ=255) aTCTGATGCCTGGATTATGCGTCTTGCCTGATCCaaa < 1:3110544/37‑1 (MQ=255) aTCTGATGCCTGGATTATGCGTCTTGCCTGATCCaaa < 1:160747/37‑1 (MQ=255) aTCTGATGCCTGGATTATGCGTCTTGCCTGATCCaaa < 1:2061519/37‑1 (MQ=255) aTCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCGGCATGGCGGGTTATTTGTCCTg > 1:3381899/1‑71 (MQ=255) aTCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCGGCATGGCGGGTTATTTGTCCTg > 1:1676546/1‑71 (MQ=255) tCTGATGCCTGGATTATGCGTCTTGCCTGATCCaaa < 1:896423/36‑1 (MQ=255) aTGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCGGCATGGc > 1:1940963/1‑51 (MQ=255) gCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCGGCATGGCGGGTTATTTGTCCTGGTTATTc > 1:4299981/1‑71 (MQ=255) gCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCGGCATGGCGGGTTATTTGTCCTGGTTATTc > 1:2308470/1‑71 (MQ=255) gCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCGGCATGGCGGGTTATTTGTCCTGGTTATTc > 1:2241432/1‑71 (MQ=255) cTGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCGGCATGGCGGGTTATTTGTCCTGGTTATTccc < 1:4268722/71‑1 (MQ=255) tGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCGGCATGGCGGGTTATTTGTCCTGGt < 1:2262931/63‑1 (MQ=255) tGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCGGCATGGCGGGTTATTTGTCCTGGt < 1:2332397/63‑1 (MQ=255) tGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCGGCATGGCGGGTTATTTGTCCTGGt < 1:766644/63‑1 (MQ=255) gATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCGGCa > 1:2988299/1‑40 (MQ=255) gATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCGGCa > 1:500140/1‑40 (MQ=255) gATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCGGCa > 1:107967/1‑40 (MQ=255) gATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCGGCATGGCGGGTTATTTGTCCTGGTTATTCCCCCg > 1:1201416/1‑71 (MQ=255) gATTATGCGTCTTGCCTGATACAAAAAGAACCCGTCGGCa > 1:2708150/1‑40 (MQ=255) gCGTCTTGCCTGATCCAAAAAGAACCCGTCGGCATGGCGGGTTATTTGTCCTGGTTATTCCCCCGTTGTa > 1:196018/1‑70 (MQ=255) tCTTGCCTGATCCAAAAAGAACCCGTCGGCATGGCGGGTTATTTGTCCTGGTTATTCCCCCGTTGTAAAAt < 1:490140/71‑1 (MQ=255) cTTGCCTGATCCAAAAAGAACCCGTCGGCATGGCGGGTTa < 1:1377929/40‑1 (MQ=255) ttGCCTGATCCAAAAAGAACCCGTCGGCATGGCGGGTTAtt > 1:1538673/1‑41 (MQ=255) | CTAAATGGCGAACACTCTGTCCTTTACCGTACCCGTTACGATCAACAATCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCGGCATGGCGGGTTATTTGTCCTGGTTATTCCCCCGTTGTAAAAT > minE/1225855‑1225993 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |