| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,515,612 | G→A | 10.9% | S135L (TCA→TTA) | cvpA ← | membrane protein required for colicin V production |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,515,612 | 0 | G | A | 10.9% | 188.6 / 11.9 | 64 | S135L (TCA→TTA) | cvpA | membrane protein required for colicin V production |
| Reads supporting (aligned to +/- strand): ref base G (28/29); new base A (4/3); total (32/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AAACTTGACGAGCTTTGCAGATAATCAAAAAAACATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGTAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAAC > minE/1515543‑1515679 | aaaCTTGACGAGCTTTGCAGATAATCAAAAAAACATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGa < 1:1313561/71‑1 (MQ=255)aaaCTTGACGAGCTTTGCAGATAATCAAAAAAACATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGa < 1:2121717/71‑1 (MQ=255) ttGACGAGCTTTGCAGATAATCAAAAAAACATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTg > 1:4488989/1‑71 (MQ=255) ttGACGAGCTTTGCAGATAATCAAAAAAACATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTg > 1:3181876/1‑71 (MQ=255) aCGAGCTTTGCAGATAATCAAAAAAACATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTc < 1:370359/71‑1 (MQ=255) aCGAGCTTTGCAGATAATCAAAAAAACATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTc < 1:3199788/71‑1 (MQ=255) cGAGCTTTGCAGATAATCAAAAAAACATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTc < 1:1876323/70‑1 (MQ=255) tGCAGATAATCAAAAAAACATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTc > 1:3931873/1‑71 (MQ=255) cAGATAATCAAAAAAACATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGc > 1:4004905/1‑71 (MQ=255) cAGATAATCAAAAAAACATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGc > 1:1739177/1‑71 (MQ=255) gATAATCAAAAAAACATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCtt < 1:4338920/71‑1 (MQ=255) gATAATCAAAAAAACATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCtt < 1:2404010/71‑1 (MQ=255) aTAATCAAAAAAACATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCttt > 1:1979979/1‑71 (MQ=255) tAATCAAAAAAACATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCttt < 1:457021/70‑1 (MQ=255) aaTCAAAAAACCATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTc > 1:2805215/1‑71 (MQ=255) aaTCAAAAAAACATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCtttt > 1:4619063/1‑70 (MQ=255) aaTCAAAAAAACATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTc > 1:4318263/1‑71 (MQ=255) aaTCAAAAAAACATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTc > 1:4139084/1‑71 (MQ=255) aaTCAAAAAAACATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTc > 1:2597708/1‑71 (MQ=255) aTCAAAAAAACATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTc < 1:4569285/70‑1 (MQ=255) aTCAAAAAAACATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTc < 1:2291759/70‑1 (MQ=255) tCAAAAAAACATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGa < 1:2026969/47‑1 (MQ=255) tCAAAAAAACATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGa < 1:2268818/47‑1 (MQ=255) tCAAAAAAACATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGa < 1:3874826/47‑1 (MQ=255) tCAAAAAAACATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGa < 1:3007757/47‑1 (MQ=255) tCAAAAAAACATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGt > 1:2566718/1‑58 (MQ=255) aaaaaaaCATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTg > 1:1520839/1‑49 (MQ=255) aaaaaaaCATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTg > 1:4736554/1‑49 (MQ=255) aCATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCtttt > 1:2006008/1‑60 (MQ=255) aCATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCtttt > 1:2773489/1‑60 (MQ=255) tgatgaTAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGt > 1:3486812/1‑45 (MQ=255) tgatAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACAcccc < 1:2022925/61‑1 (MQ=255) atAAAACTGAATTGCGGGATCAGCTGTGATTTGCt > 1:1756991/1‑35 (MQ=255) atAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGTAAAGGAGTc < 1:2965375/71‑1 (MQ=255) atAAAACTGAATTGCGGGATCAGCTGTAATTTGCTCCAGTCtt < 1:2308435/43‑1 (MQ=255) atAAAACTGAATTGCGGGATCAGCTGTAATTTGCTCCAGTCtt < 1:3331424/43‑1 (MQ=255) atAAAACTGAATTGCGGGATCAGCTGTAATTTGCTCCAGTAtt < 1:3261911/43‑1 (MQ=38) tAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGTAAAGGAGTc < 1:104820/70‑1 (MQ=255) tAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGTAAAGGAGTc < 1:1334322/70‑1 (MQ=255) aaaaCTGAATTGCGGGATCAGCTGTGATTTGCTCCAGt > 1:4035569/1‑38 (MQ=255) aaaaCTGAATTGCGGGATCAGCTGTAATTTGCTCCAGTCTTCg > 1:1255848/1‑43 (MQ=255) aaaaCTGAATTGCGGGATCAGCTGTAATTTGCTCCAGTCTTCg > 1:384815/1‑43 (MQ=255) aaaaCTGAATTGCGGGATCAGCTGTAATTTGCTCCAGTCTTCg > 1:1250386/1‑43 (MQ=255) aaaaCTGAATTGCGGGATCAGCTGTAATTTGCTCCAGTCTTCg > 1:4362712/1‑43 (MQ=255) aaaCTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGTAAAGGAGTCgag < 1:1598720/71‑1 (MQ=255) aaCTGAATTGCGGGATCAGCTGTGATTTGCTCCAGt > 1:1212509/1‑36 (MQ=255) aaCTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCg > 1:4054237/1‑48 (MQ=255) aaCTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGTAAAGGAGTCgag > 1:3473187/1‑70 (MQ=255) aaCTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGTAAAGGAGTCgag > 1:3317677/1‑70 (MQ=255) aaCTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGTAAAGGAGTCgag > 1:3833195/1‑70 (MQ=255) tGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTc > 1:4079017/1‑44 (MQ=255) tGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTc > 1:1897216/1‑44 (MQ=255) tGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTc > 1:1809296/1‑44 (MQ=255) tGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTc > 1:1902773/1‑44 (MQ=255) gCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGTAAAGGAGTCGAGAAAGAagag < 1:3594685/70‑1 (MQ=255) gCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGTAAAGGAGTCGAGAAAGAagag < 1:327547/70‑1 (MQ=255) gCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGTAAAGGAGTCGAGAAAGAagag < 1:4117062/70‑1 (MQ=255) gCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGTAAAGGAGTCGAGAAAGAagag < 1:2448822/70‑1 (MQ=255) ggATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGTAAAGGAGTCGAGAATGAAGAGAAt < 1:4328983/70‑1 (MQ=255) aTCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGTAAAGGAGTCGAGAAAGAAGAGAATGgc < 1:733929/71‑1 (MQ=255) cAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGTAAAGGAGTCGagaaagaa < 1:2328901/60‑1 (MQ=255) aGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGTAAAGGAGTCGagaaagaa < 1:1121443/59‑1 (MQ=255) cTGTGATTTGGTCCTGTCTTCGGTTTTCGACAGCCCGGTAATGGAGTCGagaaag < 1:3460499/55‑1 (MQ=255) cTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGTAAAGGAGTCGagaaag < 1:4033889/55‑1 (MQ=255) tgtgATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGTAAAGGAGTCg < 1:3780605/48‑1 (MQ=255) tgtgATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGTAAAGGAGTCg < 1:3185365/48‑1 (MQ=255) gtgATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGTAAAGGAGTCGAGAAAGAAGAGAATGGCAGcaac > 1:1299505/1‑70 (MQ=255) | AAACTTGACGAGCTTTGCAGATAATCAAAAAAACATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGTAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAAC > minE/1515543‑1515679 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |