| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,613,318 | A→G | 7.0% | F353S (TTC→TCC) | eutH ← | predicted inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,613,318 | 0 | A | G | 7.0% | 209.6 / 6.5 | 71 | F353S (TTC→TCC) | eutH | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base A (37/29); new base G (2/3); total (39/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.52e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.41e-01 | |||||||||||
CCGACAATCATCGGGAAGATCATGGCGTTCATGTTGGCAGCGGCGAAGCCTAAGTGGTCGCCCAGGGCGAAAGCAGCGGAAACGGCGAAGGCGCAGTTGATGACTTTGCCGCGGGTATCCATCTGCTTCATCATGCCG > minE/1613249‑1613386 | ccGACAATCATCGGGAATATCATGGCGTTCATGTTGGCAGCGGCGAAGCCTAAGTGGTCGCCCAGGGCGa > 1:3978735/1‑70 (MQ=255)ccGACAATCATCGGGAAGATCATGGCGTTCATGTTGGCAGCGGCGAAGCCTAAGTGGTCGCCCAGGGCGa > 1:3230653/1‑70 (MQ=255)ccGACAATCATCGGGAAGATCATGGCGTTCATGTTGGCAGCGGCGAAGCCTAAGTGGTCGCCCAGGGCGa > 1:764681/1‑70 (MQ=255)ccGACAATCATCGGGAAGATCATGGCGTTCATGTTGGCAGCGGCGAAGCCTAAGTGGTCGCCCAGGGCGa < 1:3578205/70‑1 (MQ=255)ccGACAATCATCGGGAAGATCATGGCGTTCATGTTGGCAGCGGCGAAGCCTAAGTGGTCGCCCAGGGCGa > 1:364070/1‑70 (MQ=255) cAATCATCGGGAAGATCATGGCGTTCATGTTGGCAGCGGCGAAGCCTAAGTGGTCGCCCAGGGCGAAagca < 1:2954110/71‑1 (MQ=255) aaTCATCGGGAAGATCATGGCGTTCATGTTGGCAGCGGCGAAGCCTAAGTGGTCGCCCAGGGCGAAag > 1:4403211/1‑68 (MQ=255) atcGGGAAGATCATGGCGTTCATGTTGGCAGCGGCGAAGCCTAAGTGGTCGCCCAGGGCGAAAGCAGCGGa < 1:34999/71‑1 (MQ=255) gggAAGATCATGGCGTTCATGTTGGCAGCGGCGAAGCCTAAGTGGTCGCCCAGGGCGAAAGCAGCGGaaa < 1:601292/70‑1 (MQ=255) aGATCATGGCGTTCATGTTGGCAGCGGCGAGGCCTAAGTGGTCGCCCAGGGCGAAAGCAGCGGAAACGGCg < 1:4358247/71‑1 (MQ=255) aGATCATGGCGTTCATGTTGGCAGCGGCGAAGCCTAAGTGGTCGCCCAGGGCGAAAGCAGCGGAAACGGCg < 1:3170280/71‑1 (MQ=255) aGATCATGGCGTTCATGTTGGCAGCGGCGAAGCCTAAGTGGTCGCCCAGGGCGAAAGCAGCGGAAACGGCg < 1:29239/71‑1 (MQ=255) aTCATGGCGTTCATGTTGGCAGCGGCGAAGCCTAAGTGGTCGCCCAGGGCGAAAGCAGCGGa > 1:4619239/1‑62 (MQ=255) aTCATGGCGTTCATGTTGGCAGCGGCGAAGCCTAAGTGGTCGCCCAGGGCGAAAGCAGCGGa > 1:4235575/1‑62 (MQ=255) aTCATGGCGTTCATGTTGGCAGCGGCGAAGCCTAAGTGGTCGCCCAGGGCGAAAGCAGCGGa > 1:4663427/1‑62 (MQ=255) aTCATGGCGTTCATGTTGGCAGCGGCGAAGCCTAAGTGGTCGCCCAGGGCGAAAGCAGCGGa > 1:4212955/1‑62 (MQ=255) cATGGCGTTCATGTTGGCAGCGGCGAAGCCTAAGTGGTCGCCCAGGGCGAAAGCAGCGGAAACGGCGAAgg > 1:1870585/1‑71 (MQ=255) tGGCGTTCATGTTGGCAGCGGCGAAGCCTAAGTGGTCGCCCAGGGCGAAAGCAGCGGAAACGGCGAAGgcg > 1:3184626/1‑71 (MQ=255) gCGTTCATGTTGGCAGCGGCGAAGCCTAAGTGGTCGCCCAGGGCGAAAGCAGCGGAAACGGCGAAGgcg > 1:1777509/1‑69 (MQ=255) gCGTTCATGTTGGCAGCGGCGAAGCCTAAGTGGTCGCCCAGGGCGAAAGCAGCGGAAACGGCGAAGgcg > 1:3383079/1‑69 (MQ=255) ttCATGTTGGCAGCGGCGAAGCCTAAGTGGTCGCCCAGGGCGAAAGCAGCGGAAACGGCGAAGGCGCAGtt < 1:1813397/71‑1 (MQ=255) ttCATGTTGGCAGCGGCGAAGCCTAAGTGGTCGCCCAGGGCGAAAGCAGCGGAAACGGCGAAGGCGCAGtt < 1:3236263/71‑1 (MQ=255) ttCATGTTGGCAGCGGCGAAGCCTAAGTGGTCGCCCAGGGCGAAAGCAGCGGAAACGGCGAAGGCGCAGtt < 1:4633175/71‑1 (MQ=255) cATGTTGGCAGCGGCGAAGCCTAAGTGGTCGCCCAGGGCGaaa > 1:3544519/1‑43 (MQ=255) cATGTTGGCAGCGGCGAAGCCTAAGTGGTCGCCCAGGGCGaaa > 1:1673388/1‑43 (MQ=255) cATGTTGGCAGCGGCGAAGCCTAAGTGGTCGCCCAGGGCGaaa > 1:2309559/1‑43 (MQ=255) cATGTTGGCAGCGGCGAAGCCTAAGTGGTCGCCCAGGGCGaaa > 1:85991/1‑43 (MQ=255) cATGTTGGCAGCGGCGAAGCCTAAGTGGTCGCCCAGGGCGGAAGCAGCGGAAACGGCGAAGGCGCAGTtga > 1:2028695/1‑71 (MQ=255) aTGTTGGCAGCGGCGAAGCCTAAGTGGTCGCCCAGGGCGAAAGCAGCGGAAACGGCGAAGgcg < 1:1367794/63‑1 (MQ=255) aTGTTGGCAGCGGCGAAGCCTAAGTGGTCGCCCAGGGCGAAAGCAGCGGAAACGGCGAAGGCGCAGTtgat > 1:2004945/1‑71 (MQ=255) aTGTTGGCAGCGGCGAAGCCTAAGTGGTCGCCCAGGGCGAAAGCAGCGGAAACGGCGAAGGCGCAGTtgat > 1:1990977/1‑71 (MQ=255) aTGTTGGCAGCGGCGAAGCCTAAGTGGTCGCCCAGGGCGAAAGCAGCGGAAACGGCGAAGGCGCAGTtga > 1:4105387/1‑70 (MQ=255) aTGTTGGCAGCGGCGAAGCCTAAGTGGTCGCCCAGGGCGAAAGCAGCGGAAACGGCGAAGGCGCAGTtga < 1:2641312/70‑1 (MQ=255) ttGGCAGCGGCGAAGCCTAAGTGGTCGCCCAGGGCGGAAGCAGCGGAAACGGCGAAGGCGCAGTtgatga < 1:4184952/70‑1 (MQ=255) ttGGCAGCGGCGAAGCCTAAGTGGTCGCCCAGGGCGAAAGCAGCGGAAACg > 1:890587/1‑51 (MQ=255) ttGGCAGCGGCGAAGCCTAAGTGGTCGCCCAGGGCGAAAGCAGCGGAAACGGCGa > 1:2026665/1‑55 (MQ=255) ttGGCAGCGGCGAAGCCTAAGTGGTCGCCCAGGGCGAAAGCAGCGGAAACGGCGa > 1:2338373/1‑55 (MQ=255) ttGGCAGCGGCGAAGCCTAAGTGGTCGCCCAGGGCGAAAGCAGCGGAAACGGCGa > 1:2659576/1‑55 (MQ=255) tGGCAGCGGCGAAGCCTAAGTGGTCGCCCAGGGCGAAAGCAGCGGAAACGGCGAAGGCGCAGTTGATGACt > 1:4195015/1‑71 (MQ=255) tGGCAGCGGCGAAGCCTAAGTGGTCGCCCAGGGCGAAAGCAGCGGAAACGGCGAAGGCGCAGTTGATGACt > 1:880223/1‑71 (MQ=255) tGGCAGCGGCGAAGCCTAAGTGGTCGCCCAGGGCGAAAGCAGCGGAAACGGCGAAGGCGCAGTTGATGACt > 1:4482328/1‑71 (MQ=255) aGCGGCGAAGCCTAAGTGGTCGCCCAGGGCGAAAGCAGCGGAAACGGCGAAGGCGCAGTtgat < 1:3586298/63‑1 (MQ=255) gcggcgAAGCCTAAGTGGTCGCCCAGGGCGAAAGCAGCGGAAACGGCGAAGGCGCAGTTGATGAc < 1:1003063/65‑1 (MQ=255) gcggcgAAGCCTAAGTGGTCGCCCAGGGCGAAAGCAGCGGAAACGGCGAAGGCGCAGTTGATGAc < 1:1273993/65‑1 (MQ=255) gcggcgAAGCCTAAGTGGTCGCCCAGGGCGAAAGCAGCGGAAACGGCGAAGGCGCAGTTGATGAc < 1:3506369/65‑1 (MQ=255) cggcgAAGCCTAAGTGGTCGCCCAGGGCGAAAGCAGCGGAAACGGCGAAGGCGCAGtt < 1:2549887/58‑1 (MQ=255) cggcgAAGCCTAAGTGGTCGCCCAGGGCGAAAGCAGCGGAAACGGCGAAGGCGCAGtt < 1:4178327/58‑1 (MQ=255) cggcgAAGCCTAAGTGGTCGCCCAGGGCGAAAGCAGCGGAAACGGCGAAGGCGCAGtt < 1:682950/58‑1 (MQ=255) cggcgAAGCCTAAGTGGGCGCCCAGGGCGAAAGCAGCGGAAACGGCGAAGGCGCAGTtgat < 1:1140925/61‑1 (MQ=255) ggcgAAGCCTAAGTGGTCGCCCAGGGCGAAAGCAGCGGAAACGGCGAAGGCGCAGTTGATGACTTTGCcgc > 1:1503552/1‑71 (MQ=255) gcgAAGCCTAAGTGGTCGCCCAGGGCGAAAGCAGCGGAAACGGCGAAGGCGCAGTTGATGACTTTGCcgcg > 1:650608/1‑71 (MQ=255) aGCCTAAGTGGTCGCCCAGGGCGAAAGCAGCGGAAACGGCGAAGGCGCAGTTGATGACTTTGCCGCGGGTa > 1:1269144/1‑71 (MQ=255) aGCCTAAGTGGTCGCCCAGGGCGAAAGCAGCGGAAACGGCGAAGGCGCAGTTGATGACTTTGCCGCGGGTa > 1:1325086/1‑71 (MQ=255) tAAGTGGTCGCCCAGGGCGAAAGCAGCGGAAACGGCGaa < 1:2489488/39‑1 (MQ=255) tAAGTGGTCGCCCAGGGCGAAAGCAGCGGAAACGGCGAAGGCGCAGTTGATGACTTTGCCGCGGGTATCCa > 1:1350836/1‑71 (MQ=255) aGTGGTCGCCCAGGGCGAAAGCAGCGGAAACGGCGAAGGCGCAGTTGATGACTTTGCCGCGGGTATCCa < 1:3490095/69‑1 (MQ=255) aGTGGTCGCCCAGGGCGAAAGCAGCGGAAACGGCGAAGGCGCAGTTGATGACTTTGCCGCGGGTATCCAt < 1:1323048/70‑1 (MQ=255) gTGGTCGCCCAGGGCGAAAGCAGCGGAAACGGCGa < 1:320304/35‑1 (MQ=255) cgTCGCCCAGGGCGAAAGCAGCGGAAACGGCGAAGGCGCAGTTGATGACTTTGCCGCGGGTa > 1:2976175/2‑62 (MQ=255) cGCCCAGGGCGAAAGCAGCGGAAACGGCGAAGGCGCAGtt < 1:4139895/40‑1 (MQ=255) gCCCAGGGCGAAAGCAGCGGAAACGGCGAAGGCGCAGTTGATGACTTTGCCGCGGGTATCCATCTGCTtca > 1:4348282/1‑71 (MQ=255) cccAGGGCGAAAGCAGCGGAAACGGCGAAGGTGCAGTtga > 1:2532848/1‑40 (MQ=255) cccAGGGCGAAAGCAGCGGAAACGGCGAAGGCGCAGTtga > 1:201562/1‑40 (MQ=255) ccAGGGCGGAAGCAGCGGAAACGGCGAAGGCGCAGTTGATGACTTTGCCGCGGGTATCCATCTGCTtcat < 1:4075282/70‑1 (MQ=255) ccAGGGCGGAAGCAGCGGAAACGGCGAAGGCGCAGTTGATGACTTTGCCGCGGGTATCCATCTGCTtcat < 1:1143531/70‑1 (MQ=255) ccAGGGCGAAAGCAGCGGAAACGGCGAAGGCGCAGTTGATGACTTTGCcg > 1:4697004/1‑50 (MQ=255) ccAGGGCGAAAGCAGCGGAAACGGCGAAGGCGCAGTTGATGACTTTGCGGCGGGTATCCATCTGCTtca > 1:3112066/1‑69 (MQ=255) aGGGCGGAAGCAGCGGAAACGGCGAAGGCGCAGTTGATGACTTTGCCGCGGGTATCCATCTGCTtcatca > 1:3318732/1‑70 (MQ=255) aGGGCGAAAGCAGCGGAAACGGCGAAGGCGCAGTTGATGACTTTGCCGCGGGTATCCATCTGCTtcatca > 1:3857181/1‑70 (MQ=255) aGGGCGAAAGCAGCGGAAACGGCGAAGGCGCAGTTGATGACTTTGCCGCGGGTATCCATCTGCTTcatcat > 1:3925957/1‑71 (MQ=255) aGGGCGAAAGCAGCGGAAACGGCGAAGGCGCAGTTGATGACTTTGCCGCGGGTATCCATCTGCTTcatcat > 1:3673506/1‑71 (MQ=255) aGGGCGAAAGCAGCGGAAACGGCGAAGGCGCAGTTGATGACTTTGCCGCGGGTATCCATCTGCTTcatcat > 1:2676068/1‑71 (MQ=255) aGGGCGAAAGCAGCGGAAACGGCGAAGGCGCAGTTGATGACTTTGCCGCGGGTATCCATCTGCTTcatcat > 1:1113294/1‑71 (MQ=255) gggCGAAAGCAGCGGAAACGGCGAAGGCGCAGTTGATGAc < 1:3466278/40‑1 (MQ=255) ggCGAAAGCAGCGGAAACGGCGAAGGCGCAGTTGATGACTTTGCCGCGGGTATCCATCTGCTTCATCATGc > 1:4259493/1‑71 (MQ=255) ggCGAAAGCAGCGGAAACGGCGAAGGCGCAGTTGATGACTTTGCCGCGGGTATCCATCTGCTTCATCATGc > 1:2529882/1‑71 (MQ=255) cGAAAGCAGCGGAAACGGCGAAGGCGCAGTTGATGACTTTGCCGCGGGTATCCATCTGCTTCATCATGCCg < 1:4283595/71‑1 (MQ=255) cGAAAGCAGCGGAAACGGCGAAGGCGCAGTTGATGACTTTGCCGCGGGTATCCATCTGCTTCATCATGCCg < 1:3370965/71‑1 (MQ=255) cGAAAGCAGCGGAAACGGCGAAGGCGCAGTTGATGACTTTGCCGCGGGTATCCATCTGCTTCATCATGCCg < 1:1933686/71‑1 (MQ=255) cGAAAGCAGCGGAAACGGCGAAGGCGCAGTTGATGACTTTGCCGCGGGTATCCATCTGCTTCATCATGCCg < 1:1339516/71‑1 (MQ=255) | CCGACAATCATCGGGAAGATCATGGCGTTCATGTTGGCAGCGGCGAAGCCTAAGTGGTCGCCCAGGGCGAAAGCAGCGGAAACGGCGAAGGCGCAGTTGATGACTTTGCCGCGGGTATCCATCTGCTTCATCATGCCG > minE/1613249‑1613386 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |