| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 145,530 | T→A | 14.3% | T233S (ACA→TCA) | sfsA ← | predicted DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 145,530 | 0 | T | A | 14.3% | 169.6 / 19.8 | 63 | T233S (ACA→TCA) | sfsA | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base T (21/33); new base A (4/5); total (25/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.19e-01 | |||||||||||
GGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCT > minE/145466‑145597 | ggAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCgg > 1:3803016/1‑71 (MQ=255) aaGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGt > 1:275595/1‑71 (MQ=255) aaGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGt < 1:2727128/71‑1 (MQ=255) gCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGa < 1:4465598/71‑1 (MQ=255) cGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGAt < 1:2279102/71‑1 (MQ=255) gTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGt < 1:4468428/57‑1 (MQ=255) gTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGt < 1:3849317/57‑1 (MQ=255) gTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGt < 1:2777874/57‑1 (MQ=255) gTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGt < 1:1785464/57‑1 (MQ=255) gTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGAAACCGGCAGTGAtt < 1:2233031/71‑1 (MQ=255) tATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGAtt < 1:4639385/70‑1 (MQ=255) tATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGAtt < 1:1713358/70‑1 (MQ=255) aTTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTa < 1:1912536/56‑1 (MQ=255) tttGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTa < 1:1162096/55‑1 (MQ=255) tGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGAtttttt < 1:1243227/70‑1 (MQ=255) gcgcACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTaa > 1:547909/1‑53 (MQ=255) gcgcACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGAttt > 1:2068017/1‑66 (MQ=255) gcgcACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGAttt > 1:4680531/1‑66 (MQ=255) gcACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGAttttt < 1:883206/66‑1 (MQ=255) gcACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGAttttt < 1:3003405/66‑1 (MQ=255) gcgACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCa > 1:1444521/1‑71 (MQ=255) gcgACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGAAACCGGCAGTGATTTTTTAAGAGCCa > 1:2966577/1‑71 (MQ=255) gcgACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGAAACCGGCAGTGATTTTTTAAGAGCCa > 1:4574/1‑71 (MQ=255) ccAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGcc > 1:1574409/1‑71 (MQ=255) cAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCt > 1:4472967/1‑71 (MQ=255) aaTGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCgg < 1:4581925/43‑1 (MQ=255) aaTGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCgg < 1:1271974/43‑1 (MQ=255) gTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGcc < 1:336693/59‑1 (MQ=255) aaTTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGa < 1:1323642/70‑1 (MQ=255) ttAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCGGAAAt < 1:554266/71‑1 (MQ=255) ttAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAAt < 1:3257191/71‑1 (MQ=255) ttAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAAt < 1:1233054/71‑1 (MQ=255) ttAACCAGTTACTTACTTTACTACAATGAAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAAt > 1:1870773/1‑71 (MQ=255) aaCCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAAtt < 1:4585183/70‑1 (MQ=255) aCCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGa < 1:2694799/65‑1 (MQ=255) ccAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTcc > 1:2405386/1‑71 (MQ=255) gTTACTTACTTTACTACAATGTAACCGTCAGTGAtttttt < 1:4428680/40‑1 (MQ=255) gTTACTTACTTTACTACAATGTAACCGGCAGTGAtttttt < 1:811866/40‑1 (MQ=255) gTTACTTACTTTACTACAATGTAACCGGCAGTGAttttt < 1:1380903/39‑1 (MQ=255) gTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCt < 1:3403509/71‑1 (MQ=255) ttacttacTTTACTACAATGTAACCGGCAGTGAtttttt < 1:3211955/39‑1 (MQ=255) tacttactTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCa > 1:3641154/1‑46 (MQ=255) tacttactTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCa > 1:117935/1‑46 (MQ=255) tacttactTTACTACAATGTAACCGGCAGTGATTATTTAAGAGcc > 1:3367934/1‑45 (MQ=255) acttacttTACTACAATGTAACCGGCAGTGAtttttt < 1:2525582/37‑1 (MQ=255) acttacttTACTACAATGTAACCGGCAGTGATTTTTTaa > 1:1559530/1‑39 (MQ=255) acttacttTACTACAATGTAACCGGCAGTGATTTTTTaa > 1:1876472/1‑39 (MQ=255) acttacttTACTACAATGTAACCGGCAGTGATTTTTTaa > 1:2621057/1‑39 (MQ=255) acttacttTACTACAATGTAACCGGCAGTGATTTTTTaa > 1:408648/1‑39 (MQ=255) cttacttTACTACAATGTAACCGGCAGTTATTTTTTAAGAGCCATGCCTTCAGCAGAAAt < 1:2132136/60‑1 (MQ=255) cttacttTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAAt < 1:3828424/60‑1 (MQ=255) actttactACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTa < 1:1254699/69‑1 (MQ=255) actttactACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTa < 1:1200427/69‑1 (MQ=255) tttactACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGc > 1:3411024/1‑70 (MQ=255) tactacAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAAt < 1:2876018/53‑1 (MQ=255) tactacAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCttt > 1:238038/1‑62 (MQ=255) tactacAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGc > 1:1343809/1‑68 (MQ=255) tactacAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGc > 1:2116401/1‑68 (MQ=255) ctacAATGAAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGt < 1:200597/62‑1 (MQ=255) ctacAATGAAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGt < 1:291248/62‑1 (MQ=255) ctacAATGAAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGt < 1:82469/62‑1 (MQ=255) ctacAATGAAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGt < 1:1698372/62‑1 (MQ=255) tacAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAAt > 1:3547479/1‑71 (MQ=255) acAATGAAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTaa > 1:2147704/1‑62 (MQ=255) cAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAAt < 1:1036391/69‑1 (MQ=255) cAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAAt < 1:870925/69‑1 (MQ=255) aTGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCt < 1:1234141/71‑1 (MQ=255) | GGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCT > minE/145466‑145597 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |