| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,735,602 | T→C | 11.0% | E157E (GAA→GAG) | era ← | membrane‑associated, 16S rRNA‑binding GTPase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,735,602 | 0 | T | C | 11.0% | 227.5 / 17.7 | 82 | E157E (GAA→GAG) | era | membrane‑associated, 16S rRNA‑binding GTPase |
| Reads supporting (aligned to +/- strand): ref base T (34/39); new base C (5/4); total (39/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.30e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.97e-01 | |||||||||||
GGAAGTGATGAGTCGCTTCAGGTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGCCAGGAACTGCAGGTGCGGCAGCAG > minE/1735532‑1735670 | ggAAGTGATGAGTCGCTTCAGGTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGtt > 1:2260312/1‑71 (MQ=255)ggAAGTGATGAGTCGCTTCAGGTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGtt > 1:4040201/1‑71 (MQ=255) gAAGTGATGAGTCGCTTCAGGTAGATGCTTACGCCCGATTGCCGCAATAGTGTCAACATTCAGCCCGGttt > 1:1436114/1‑71 (MQ=255) gTGATGAGTCGCTTCAGGTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCgg > 1:542882/1‑71 (MQ=255) atgaGTCGCTTCAGGTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGc < 1:286712/69‑1 (MQ=255) atgaGTCGCTTCAGGTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTCTCGGc < 1:4414510/69‑1 (MQ=255) tgaGTCGCTTCAGGTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGc < 1:1767592/68‑1 (MQ=255) cGCTTCAGGTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTCTCGGCAGAGATTg > 1:2309235/1‑71 (MQ=255) cGCTTCAGGTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTCTCGGCAGAGATTg > 1:1961905/1‑71 (MQ=255) gCTTCAGGTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTgg < 1:3054077/71‑1 (MQ=255) gCTTCAGGTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTgg < 1:1564464/71‑1 (MQ=255) cTTCAGGTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGc < 1:1535428/71‑1 (MQ=255) cTTCAGGTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGc < 1:3376615/71‑1 (MQ=255) cAGGTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCa > 1:2569818/1‑59 (MQ=255) cAGGTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCa > 1:3835602/1‑59 (MQ=255) cAGGTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCa > 1:4706895/1‑59 (MQ=255) cAGGTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCa > 1:4687029/1‑59 (MQ=255) tAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGatat < 1:4307365/71‑1 (MQ=255) gATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCATAGATTGGCACGATATc < 1:3383294/70‑1 (MQ=255) gATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATc < 1:3763256/70‑1 (MQ=255) aTGCTTACGGACGATTGCCGCAATAGTGTCAACATTCAGCCCGGttt < 1:3146755/47‑1 (MQ=255) aTGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGttt < 1:3352437/47‑1 (MQ=255) aTGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGttt < 1:3813019/47‑1 (MQ=255) aTGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGttt < 1:3109318/47‑1 (MQ=255) aTGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGttt < 1:2664069/47‑1 (MQ=255) aTGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGttt < 1:3060940/47‑1 (MQ=255) aTGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGttt < 1:2061434/47‑1 (MQ=255) aTGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGttt < 1:785108/47‑1 (MQ=255) gCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAg > 1:1217208/1‑70 (MQ=255) gCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAg > 1:3627026/1‑70 (MQ=255) gCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAg > 1:3826865/1‑70 (MQ=255) gCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAg > 1:3451761/1‑70 (MQ=255) gCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAg > 1:2659620/1‑70 (MQ=255) ttACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGa < 1:1005742/67‑1 (MQ=255) ttACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAgg < 1:3558688/69‑1 (MQ=255) cGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGtt > 1:1408170/1‑71 (MQ=255) cGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGtt > 1:2573264/1‑71 (MQ=255) cGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGt > 1:3943847/1‑70 (MQ=255) cGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGt > 1:3761683/1‑70 (MQ=255) cGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTCTCGGCAgaga > 1:4653903/1‑49 (MQ=255) cGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTCTCGGCAgaga > 1:4269339/1‑49 (MQ=255) cGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTCTCGGCAgaga > 1:3282150/1‑49 (MQ=255) cACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTc > 1:2640955/1‑70 (MQ=255) cACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCa < 1:3969772/71‑1 (MQ=255) cACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCa < 1:2152006/71‑1 (MQ=255) cACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCa < 1:588615/71‑1 (MQ=255) cACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCa < 1:2468675/71‑1 (MQ=255) aCGATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCa < 1:374947/70‑1 (MQ=255) cGATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATc > 1:2228539/1‑71 (MQ=255) gATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCt < 1:1248830/71‑1 (MQ=255) gATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCt < 1:3814548/71‑1 (MQ=255) gATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCt < 1:3327859/71‑1 (MQ=255) gATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCt > 1:194349/1‑71 (MQ=255) gATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCt < 1:2515718/71‑1 (MQ=255) gATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCt < 1:2841964/71‑1 (MQ=255) aTTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCAt > 1:3269167/1‑68 (MQ=255) aTTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCAt > 1:3031455/1‑68 (MQ=255) aTTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTg > 1:1443294/1‑71 (MQ=255) ccGCAATAGTGTCAACATTCAGCCCGGTTTCGGCtgag < 1:3771919/38‑1 (MQ=255) gCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGAt > 1:3716053/1‑38 (MQ=255) gCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGc < 1:4698178/71‑1 (MQ=255) gCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGc < 1:2284834/71‑1 (MQ=255) gCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGc < 1:433706/71‑1 (MQ=255) gCAATAGTGTCAACATTCAGCCCGGTCTCGGCAGAGATTGGCACGATATCGAgg < 1:3322933/54‑1 (MQ=255) tAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGCCAgg < 1:803499/71‑1 (MQ=255) tgtCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTg < 1:363288/63‑1 (MQ=255) tgtCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTg < 1:4601650/63‑1 (MQ=255) tgtCAACATTCAGCCCGGTCTCGGCAGAGATTGGCACGATATCGAGGAAGTTc < 1:4545974/53‑1 (MQ=255) tgtCAACATTCAGCCCGGTCTCGGCAGAGATTGGCACGATATCGAGGAAGTTc < 1:2809755/53‑1 (MQ=255) tCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGcc > 1:814491/1‑63 (MQ=255) aaCATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGCCAGGAACTg > 1:751647/1‑69 (MQ=255) aaCATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGCCAGGAACTg > 1:1519449/1‑69 (MQ=255) aaCATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGCCAGGAACTg > 1:515470/1‑69 (MQ=255) aaCATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGCCAGGAACTg > 1:2101028/1‑69 (MQ=255) aaCATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGCCAGGAACTGc < 1:4241739/70‑1 (MQ=255) aTTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGaa > 1:1907088/1‑42 (MQ=255) aTTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGaa > 1:3098239/1‑42 (MQ=255) aTTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGaa > 1:2573847/1‑42 (MQ=255) ttCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAgg < 1:1793153/39‑1 (MQ=255) ttCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAgg < 1:2835376/39‑1 (MQ=255) ttCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGCCAGGAACTGCAGGTg > 1:3408008/1‑71 (MQ=255) cAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGaa > 1:4454215/1‑39 (MQ=255) aGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGCCAGGaa > 1:829221/1‑59 (MQ=255) aGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGCCAGGaa > 1:3710521/1‑59 (MQ=255) aGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGCCAGGAACTGCAGGTGCgg < 1:1045645/71‑1 (MQ=255) ccGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGCCAGGAACTGCAGGTGCGgcag < 1:4226239/71‑1 (MQ=255) ccGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGCCAGGAACTGCAGGTGCGgcag < 1:3473044/71‑1 (MQ=255) ccGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGCCAGGAACTGCAGGTGCGgcag < 1:1938032/71‑1 (MQ=255) ccGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGCCAGGAACTGCAGGTGCGgcag < 1:112932/71‑1 (MQ=255) cGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGCCAGGAACTGc < 1:255252/59‑1 (MQ=255) ggTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCt < 1:3961440/41‑1 (MQ=255) ggTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGCCAGGAACTGCAGGTGCGgcag > 1:1604827/1‑69 (MQ=255) gTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGCCAGGAACTGCAGGTGCGGcagcag < 1:2789403/71‑1 (MQ=255) tttCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGCCAGGAACTGCAGGTGCGgcag > 1:499856/1‑67 (MQ=255) | GGAAGTGATGAGTCGCTTCAGGTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGCCAGGAACTGCAGGTGCGGCAGCAG > minE/1735532‑1735670 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |