| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,824,288 | G→A | 8.1% | D452D (GAC→GAT) | hypF ← | carbamoyl phosphate phosphatase and maturation protein for [NiFe] hydrogenases |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,824,288 | 0 | G | A | 8.1% | 234.1 / 8.7 | 74 | D452D (GAC→GAT) | hypF | carbamoyl phosphate phosphatase and maturation protein for [NiFe] hydrogenases |
| Reads supporting (aligned to +/- strand): ref base G (29/39); new base A (3/3); total (32/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.58e-01 | |||||||||||
ATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGTAACGCTTCGC > minE/1824219‑1824349 | atgCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGt < 1:845121/71‑1 (MQ=255) gCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCa > 1:1109051/1‑71 (MQ=255) gCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCa > 1:720887/1‑71 (MQ=255) cAGTACCGTTTTCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCa < 1:4606304/70‑1 (MQ=255) aGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATg > 1:2077876/1‑71 (MQ=255) aGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATg > 1:162727/1‑71 (MQ=255) aCCGTTTGCGTAGGCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCATCATGca > 1:2617037/1‑70 (MQ=255) ccGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGcaga < 1:1331171/71‑3 (MQ=255) ccGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGcaca < 1:3210985/71‑1 (MQ=255) ccGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGcaca < 1:2415235/71‑1 (MQ=255) ccGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGcac < 1:1376538/70‑1 (MQ=255) ccGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGcac < 1:230331/70‑1 (MQ=255) ccGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGcac < 1:1158461/70‑1 (MQ=255) ccGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGca > 1:1153447/1‑69 (MQ=255) ccGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGca > 1:4301469/1‑69 (MQ=255) tGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGt < 1:2299363/70‑1 (MQ=255) tGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGt < 1:4631037/70‑1 (MQ=255) cGTCGGCAGATTCATTTCACGCGCCCACTGGCTTGAGACATAGCCCGGATGCGCGTCATGCACAACGTAt < 1:1767945/70‑1 (MQ=255) cGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTAtt > 1:632069/1‑71 (MQ=255) cGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGCCAGGATGCGCGTCATGCACAACGTAtt < 1:198718/71‑1 (MQ=255) tCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGc < 1:4398569/71‑1 (MQ=255) tCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGc < 1:4244354/71‑1 (MQ=255) tCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGc < 1:3046037/71‑1 (MQ=255) ggCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCgg > 1:3083874/1‑71 (MQ=255) ggCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCg < 1:1039020/70‑1 (MQ=255) aTTCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTa > 1:1450710/1‑70 (MQ=255) tCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCg < 1:2928106/63‑1 (MQ=255) tCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCg < 1:3287459/63‑1 (MQ=255) tCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCg < 1:3219127/63‑1 (MQ=255) tCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCg < 1:4409102/63‑1 (MQ=255) tCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTaaaa > 1:3745033/1‑71 (MQ=255) tCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTaa < 1:2288248/69‑1 (MQ=255) tCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTaa < 1:3126627/69‑1 (MQ=255) cATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGt < 1:2465780/56‑1 (MQ=255) cATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGt < 1:4502781/56‑1 (MQ=255) tCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCATCATGCACAACGTATTGCGGGGTAAAATCg > 1:4366508/1‑69 (MQ=255) cACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCg > 1:113487/1‑68 (MQ=255) cgcgCCCACTGGCTGGAGACATAGCCCGGATGCGCATCATGCACAACGTATTGCGGGGTAAAATCGTAGAt > 1:2231729/1‑71 (MQ=255) gcgcCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATg < 1:4426735/71‑1 (MQ=255) gcgcCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATg < 1:3321476/71‑1 (MQ=255) cgcCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGt < 1:4258615/71‑1 (MQ=255) cccACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTaaaa > 1:3580727/1‑59 (MQ=255) ccACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGtttt > 1:4468023/1‑71 (MQ=255) aCTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTg > 1:1496647/1‑70 (MQ=255) aCTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTg > 1:4745326/1‑70 (MQ=255) gtggctggAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATg < 1:893082/63‑1 (MQ=255) ggctggAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCAt > 1:3746463/1‑70 (MQ=255) gctggAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCAt < 1:4662884/69‑1 (MQ=255) gctggAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCAt > 1:3049647/1‑69 (MQ=255) gctggAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCAt < 1:2591730/69‑1 (MQ=255) gctggAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATc < 1:1175588/70‑1 (MQ=255) gctggAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATc < 1:2062473/70‑1 (MQ=255) gctggAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATc < 1:397388/70‑1 (MQ=255) gctggAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATc < 1:2118032/70‑1 (MQ=255) gagaCATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCg > 1:3802699/1‑71 (MQ=255) gagaCATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCg > 1:3983930/1‑71 (MQ=255) aCATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGTaa > 1:3111742/1‑71 (MQ=255) aCATAGCCCGGATGCGCATCATGCACAACGTATTGCGGGGTAAAATCGTTGATGttt < 1:3424063/57‑1 (MQ=255) aCATAGCCCGGATGCGCATCATGCACAACGTATTGCGGGGTAAAATCGTAGATGttt < 1:2575433/57‑1 (MQ=255) aCATAGCCCGGATGCGCATCATGCACAACGTATTGCGGGGTAAAATCGTAGATGttt < 1:3171997/57‑1 (MQ=255) cATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGCTGtt > 1:1491502/1‑55 (MQ=255) cATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGt < 1:2495351/54‑1 (MQ=255) tAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGttt > 1:2758085/1‑54 (MQ=255) tAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGttt > 1:2952266/1‑54 (MQ=255) tAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGttt > 1:571452/1‑54 (MQ=255) tAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGTAACGc < 1:2082073/71‑1 (MQ=255) gCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAg > 1:1174192/1‑46 (MQ=255) gCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGa > 1:4271988/1‑47 (MQ=255) gCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGa > 1:2965329/1‑47 (MQ=255) cGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGTAACGCTTcgc < 1:4310852/71‑1 (MQ=255) cGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGTAACGCTTcgc < 1:2734229/71‑1 (MQ=255) ggATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGAt > 1:108199/1‑44 (MQ=255) ggATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGAt > 1:1213881/1‑44 (MQ=255) tGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGt < 1:2852777/43‑1 (MQ=255) cgcgTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGt > 1:2046531/1‑55 (MQ=255) cgcgTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGt > 1:867957/1‑55 (MQ=255) gTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGTAACGc < 1:839674/57‑1 (MQ=255) | ATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGTAACGCTTCGC > minE/1824219‑1824349 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |