| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,976,997 | G→A | 6.0% | L83L (TTG→TTA) | sprT → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,976,997 | 0 | G | A | 6.0% | 223.0 / 3.4 | 67 | L83L (TTG→TTA) | sprT | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (32/31); new base A (2/2); total (34/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.34e-01 | |||||||||||
ATCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCTGGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGAGTGGAAGTGGATGATGGAAAACGTG > minE/1976927‑1977066 | aTCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTg < 1:4715035/71‑1 (MQ=255) cTGTTGGAAAACAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCTGGTATGGaa < 1:3348623/71‑1 (MQ=255) ttGGAAAACAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCTGGTATGGAAACa > 1:4306076/1‑71 (MQ=255) ttGGAAAACAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCTGGTATGGAAACa > 1:21727/1‑71 (MQ=255) tGGAAAACAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCTGGTATGGAAACAt > 1:2574149/1‑71 (MQ=255) tGGAAAACAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCTGGTATGGAAACAt > 1:2199815/1‑71 (MQ=255) ggAAAACAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCTGGTATGGAAACAt < 1:152428/70‑1 (MQ=255) aaaCAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCTGGTATGGAAACATTTCg > 1:3881665/1‑71 (MQ=255) aGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCTGGTATGGaa < 1:4093054/59‑1 (MQ=255) aGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCTGGTATGGaa < 1:4024303/59‑1 (MQ=255) aGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCTGGTATGGaa < 1:3591625/59‑1 (MQ=255) aaGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCTGGTATGGAAACATTTCGGCcgcg > 1:111112/1‑69 (MQ=255) aaGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCTGGTATGGAAACATTTCGGCcgcg > 1:816697/1‑69 (MQ=255) aaGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCTGGTATGGAAACATTTCGGCCGCGt < 1:1148427/70‑1 (MQ=255) aGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCTGGTATGGAAACATTTCGGCCGCGt < 1:2040273/69‑1 (MQ=255) gCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTg > 1:1728051/1‑42 (MQ=255) gCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCTGGTa > 1:2919628/1‑48 (MQ=255) gCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCTGGTa > 1:580552/1‑48 (MQ=255) cTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCTGGTATGGAAACATTTCgg < 1:1917930/61‑1 (MQ=255) cTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCTGGTATGGAAACATTTCgg < 1:2048920/61‑1 (MQ=255) tttATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCTGGTATGGAAACATTTCGGcc > 1:3873841/1‑61 (MQ=255) tttATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCTGGTATGGAAACATTTCGGcc > 1:1844139/1‑61 (MQ=255) tttATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCTGGTATGGAAACATTTCGGcc > 1:2906970/1‑61 (MQ=255) ttATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCTGGTATGGa < 1:373143/48‑1 (MQ=255) ttATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCTGGTATGGa < 1:1588279/48‑1 (MQ=255) ttATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCTGGTATGGAAACATTTCGGCCGCGTAg > 1:3873781/1‑66 (MQ=255) tATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCTGGTATGGAAACATTTCGGCcgcg < 1:3394415/62‑1 (MQ=255) ttGAAGAAGTGGTACCGCACGAACTGGCACATTTGCTGGTATGGAAACATTTCGGCCGCGTa > 1:2319156/1‑62 (MQ=255) ttGAAGAAGTGGTACCGCACGAACTGGCACATTTGCTGGTATGGAAACATTTCGGCCGCGTa > 1:2486696/1‑62 (MQ=255) gaagaaGTGGTACCGCACGAACTGGCACATTTGCTGGTATGGAAACATTTCGGCCGCGTAGCGCCACATgg > 1:3876871/1‑71 (MQ=255) gaagTGGTACCGCACGAACTGGCACATTTACTGGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCa > 1:1884457/1‑70 (MQ=255) gTGGTACCGCACGAACTGGCACATTTGCTGGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAaga < 1:4734324/71‑1 (MQ=255) gTGGTACCGCACGAACTGGCACATTTGCTGGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAaga < 1:197579/71‑1 (MQ=255) tGGTACCGCACGAACTGGCACATTTGCTGGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAagag < 1:3780871/71‑1 (MQ=255) gTACCGCACGAACTGGCACATTTGCTGGTATGGAAACATTTCg > 1:2801596/1‑43 (MQ=255) gTACCGCACGAACTGGCACATTTGCTGGTATGGAAACATTTCg > 1:2515506/1‑43 (MQ=255) gTACCGCACGAACTGGCACATTTGCTGGTATGGAAACATTTCg > 1:643448/1‑43 (MQ=255) ccGCACGAACTGGCACATTTGCTGGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGagtgga < 1:1584355/70‑1 (MQ=255) ccGCACGAACTGGCACATTTGCTGGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGagtgga < 1:3034173/70‑1 (MQ=255) ccGCACGAACTGGCACATTTGCTGGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGagtgga < 1:584746/70‑1 (MQ=255) cGCACGAACTGGCACATTTGCTGGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCaaa < 1:388420/62‑1 (MQ=255) cGCACGAACTGGCACATTTGCTGGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCaaa < 1:2814216/62‑1 (MQ=255) cGCACGAACTGGCACATTTGCTGGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCaaa < 1:1867977/62‑1 (MQ=255) gAACTGGCACATTTGCTGGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGAGTGGAAGTGgat < 1:1273285/71‑1 (MQ=255) gAACTGGCACATTTGCTGGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGAGTGGAAGTGgat < 1:809555/71‑1 (MQ=255) gAACTGGCACATTTGCTGGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGAGTGGAAGTGgat > 1:3574785/1‑71 (MQ=255) aaCTGGCACATTTGCTGGTATGGAAACATTTCGGCCGCGTAGCGCCACATGTCAAagag > 1:3831423/1‑59 (MQ=255) aaCTGGCACATTTGCTGGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAagag > 1:1968273/1‑59 (MQ=255) aaCTGGCACATTTGCTGGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGagtggaagtg < 1:3525448/67‑1 (MQ=255) cTGGCACATTTGCTGGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAaga > 1:3264227/1‑56 (MQ=255) cTGGCACATTTGCTGGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAaga > 1:3263711/1‑56 (MQ=255) cTGGCACATTTGCTGGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGAGTGGAAGTGgatgat > 1:2069673/1‑71 (MQ=255) cTGGCACATTTGCTGGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGAGTGGAAGTGgatgat > 1:1895744/1‑71 (MQ=255) gCACATTTGCTGGTATGGAAACATTTCGGCCGCGTAGCGCcaca > 1:2427704/1‑44 (MQ=255) gCACATTTGCTGGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGAGTGGAAGTGGATGATgg < 1:1998718/70‑1 (MQ=255) acaTTTGCTGGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGAGTGGAAGTGGATGATGGaa > 1:2793139/1‑70 (MQ=255) caTTTACTGGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGAGTGGAAGTGGATGATGGaaaa < 1:3811269/71‑1 (MQ=255) caTTTACTGGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGAGTGGAAGTGGATGATGGaaaa < 1:4378543/71‑1 (MQ=255) aTTTGCTGGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGagtggaagtg < 1:1169303/58‑1 (MQ=255) aTTTGCTGGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGagtggaagtg < 1:3637562/58‑1 (MQ=255) aTTTGCTGGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGAGTGGAAGTGGATGATGGAAAAc < 1:2985667/71‑1 (MQ=255) aTTTACTGGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGAGTGGAAGTGGATGATGGAAAAc > 1:3754060/1‑71 (MQ=255) tttGCTTGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGAGTGGAAGTGGATGATGGAAAACg < 1:4412715/71‑1 (MQ=255) tttGCTGGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGagtgg < 1:3970856/52‑1 (MQ=255) tttGCTGGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGAGTGGAAGTGGATGATGGAAAAc < 1:2864348/70‑1 (MQ=255) tGCTGGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGAGTGGAAGTGGATGATGGAAAAc > 1:3682728/1‑68 (MQ=255) tGCTGGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGAGTGGAAGTGGATGATGGAAAACGTg > 1:1086371/1‑71 (MQ=255) tGCTGGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGAGTGGAAGTCGATGATGGAAAACGTg > 1:4070986/1‑71 (MQ=255) tGCTGGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGAGTAGAAGTGGATGATGGAAAAc > 1:2907505/1‑68 (MQ=255) | ATCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCTGGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGAGTGGAAGTGGATGATGGAAAACGTG > minE/1976927‑1977066 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |