| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,061,532 | C→A | 5.0% | D13E (GAC→GAA) | rpsU → | 30S ribosomal subunit protein S21 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,061,532 | 0 | C | A | 5.0% | 345.5 / 2.6 | 98 | D13E (GAC→GAA) | rpsU | 30S ribosomal subunit protein S21 |
| Reads supporting (aligned to +/- strand): ref base C (50/43); new base A (3/2); total (53/45) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.06e-01 | |||||||||||
GGCAAACCGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTG > minE/2061462‑2061599 | ggCAAACCGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGAc > 1:3623087/1‑71 (MQ=255) aaaCCGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACg > 1:3113416/1‑69 (MQ=255) aaaCAGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACg > 1:4571508/1‑69 (MQ=255) cGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGctc < 1:435675/71‑1 (MQ=255) cGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGctc < 1:4317462/71‑1 (MQ=255) cGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGctc < 1:4012498/71‑1 (MQ=255) gAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGctct < 1:1069081/71‑1 (MQ=255) gAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGctct < 1:2179505/71‑1 (MQ=255) gAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGctct > 1:193155/1‑71 (MQ=255) tAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGt > 1:3391364/1‑71 (MQ=255) aaTCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTc > 1:274622/1‑71 (MQ=255) cAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCt > 1:3953842/1‑71 (MQ=255) aaaGGTGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGctct > 1:2954023/1‑62 (MQ=255) aaaGGTGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGctct > 1:1353859/1‑62 (MQ=255) aaaGGTGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCtt > 1:1573189/1‑71 (MQ=255) aaaGGTGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCtt < 1:4667490/71‑1 (MQ=255) aaaGGTGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCtt > 1:923647/1‑71 (MQ=255) aaaGGTGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCtt > 1:1342936/1‑71 (MQ=255) aaGGTGAGAGGCACATGCCGGTAATTAAAGTACGTGAAGACGAGCCGTTCGACGTAGCTCTGCGTCGCTTc < 1:1521630/71‑1 (MQ=255) ggTGAGAGGCACATGCCGGTTATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCaa < 1:619816/71‑1 (MQ=255) ggTGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCaa < 1:167638/71‑1 (MQ=255) gagaGGCTCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCa < 1:2361297/67‑1 (MQ=255) gagaGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCa < 1:1985972/67‑1 (MQ=255) gagaGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCa < 1:572986/67‑1 (MQ=255) gagaGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCg > 1:3612611/1‑71 (MQ=255) gagaGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCg > 1:3287774/1‑71 (MQ=255) gagaGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGACGTTCGACGTAGCTCTGCGTCGCTTCAAGCg > 1:625022/1‑71 (MQ=255) agagGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGt > 1:1975976/1‑71 (MQ=255) agagGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGt > 1:3955954/1‑71 (MQ=255) gagGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGtt > 1:2975369/1‑71 (MQ=255) agGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTc > 1:1294462/1‑71 (MQ=255) ggCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCg < 1:4207475/55‑1 (MQ=255) acaTGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGc < 1:1018230/71‑1 (MQ=255) acaTGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGc < 1:932962/71‑1 (MQ=255) aTGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGa < 1:4697634/71‑1 (MQ=255) aTGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGa < 1:2514086/71‑1 (MQ=255) aTGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGa < 1:4650931/71‑1 (MQ=255) aTGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGa < 1:1768505/71‑1 (MQ=255) tGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGt < 1:755120/40‑1 (MQ=255) tGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGt < 1:2095833/40‑1 (MQ=255) tGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGc > 1:2176984/1‑48 (MQ=255) tGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGa > 1:542875/1‑70 (MQ=255) gCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGaa > 1:281357/1‑70 (MQ=255) ccGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGaaaa < 1:2203690/71‑1 (MQ=255) ccGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGaaa < 1:2549376/70‑1 (MQ=255) ccGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGaaa > 1:1994760/1‑70 (MQ=255) ccGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGaaa < 1:622552/70‑1 (MQ=255) cGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGaaa > 1:2831723/1‑69 (MQ=255) cGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGaaa > 1:720970/1‑69 (MQ=255) ttAAAGTACGTGAAAATGAGCCGTTCGACGTAGCTCTGCGTc < 1:2218418/42‑1 (MQ=255) ttAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTc < 1:4754101/42‑1 (MQ=255) ttAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTc < 1:3722320/42‑1 (MQ=255) ttAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTc < 1:3634383/42‑1 (MQ=255) ttAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAgg < 1:1864572/70‑1 (MQ=255) ttAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGgt > 1:711071/1‑71 (MQ=255) tAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGgtg > 1:1850508/1‑71 (MQ=255) tAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGgtg > 1:307214/1‑71 (MQ=255) aaaGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCaa > 1:435663/1‑47 (MQ=255) aaaGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCaa > 1:3593242/1‑47 (MQ=255) aaaGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGgtgt > 1:364969/1‑71 (MQ=255) aaaGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGgtgt > 1:3040003/1‑71 (MQ=255) aaaGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGgtgt > 1:4416356/1‑71 (MQ=255) aaGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGgtgt < 1:2317958/70‑1 (MQ=255) cGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGc > 1:2404828/1‑59 (MQ=255) cGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGc > 1:127102/1‑59 (MQ=255) cGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTgg < 1:1992416/70‑1 (MQ=255) tGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGgtgt < 1:1463528/63‑1 (MQ=255) tGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGgtgt < 1:880993/63‑1 (MQ=255) tGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGgtgt < 1:2518852/63‑1 (MQ=255) gAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCgg > 1:2811013/1‑70 (MQ=255) aaaaCGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGgtgt < 1:2022568/61‑1 (MQ=255) aaaaCGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCgg > 1:285253/1‑69 (MQ=255) aaaaCGAGCCGTTCGAAGTCGCAATGCGTCGCTTCAAACGCACCATCGAAAAAACTGGTCTGCTGACGGaa > 1:1019933/1‑71 (MQ=255) aaaaCGAGCCGTTCGAAGTCGCAATGCGTCGCTTCAAACGCACCATCGAAAAAACTGGTCTGCTGACGGaa > 1:1449614/1‑71 (MQ=255) aaaaCGAGCCGTTCGAAGTCGCAATGCGTCGCTTCAAACGCACCATCGAAAAAACTGGTCTGCTGACGGa > 1:432435/1‑70 (MQ=255) aaaCGAGCCGTTCGACGTAGCTCTGCGTCGCTTCaa > 1:1226344/1‑36 (MQ=255) aaaCGAGCCGTTCGACGTAGCTCTGCGTCGCTTCaa > 1:1408612/1‑36 (MQ=255) aaaCGAGCCGTTCGACGTAGCTCTGCGTCGCTTCaa > 1:4305000/1‑36 (MQ=255) aaaCGAGCCGTTCGACGTAGCTCTGCGTCGCTTCaa > 1:4679386/1‑36 (MQ=255) aaaCGAGCCGTTCGAAGTCGCAATGCGTCGCTTCAAACGCACCATCGAAAAAACTGGTCTGCTGACGGaac < 1:1410787/71‑2 (MQ=255) aaaCGAGCCGTTCGAAGTCGCAATGCGTCGCTTCAAACGCACCATCGAAAAAACTGGTCTGCTGACGGaac < 1:3206717/71‑2 (MQ=255) aaCGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGt < 1:987020/71‑1 (MQ=255) aaCGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGt > 1:283093/1‑71 (MQ=255) aaCGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGt < 1:4741940/71‑1 (MQ=255) aaCGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGt < 1:4660032/71‑1 (MQ=255) aGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGaaaaa > 1:217485/1‑47 (MQ=255) aGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGaaaaa > 1:4322648/1‑47 (MQ=255) gTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTtcgtcgtc < 1:1479754/71‑1 (MQ=255) gTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTtcgtcgtc < 1:1336043/71‑1 (MQ=255) ttCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGaa < 1:4548268/39‑1 (MQ=255) cGACGTAGCTCTGCGTCGCTTCAAGCTTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTcgtcgtcgt > 1:4333830/1‑70 (MQ=255) cGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTc > 1:375935/1‑50 (MQ=255) cGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGaa > 1:353370/1‑58 (MQ=255) cGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGaa > 1:544467/1‑58 (MQ=255) cGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGaa > 1:2890048/1‑58 (MQ=255) cGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGaa > 1:946537/1‑58 (MQ=255) cGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTcgtcgtcgt < 1:3036209/70‑1 (MQ=255) cGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTcgtcgtcgt > 1:2437457/1‑70 (MQ=255) gACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTg < 1:4286524/70‑1 (MQ=255) | GGCAAACCGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTG > minE/2061462‑2061599 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |