| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,061,553 | G→A | 5.7% | K20K (AAG→AAA) | rpsU → | 30S ribosomal subunit protein S21 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,061,553 | 0 | G | A | 5.7% | 290.5 / 5.6 | 87 | K20K (AAG→AAA) | rpsU | 30S ribosomal subunit protein S21 |
| Reads supporting (aligned to +/- strand): ref base G (46/36); new base A (3/2); total (49/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01 | |||||||||||
GAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGAAAAACCGACTACCG > minE/2061485‑2061623 | gagaGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCg > 1:3287774/1‑71 (MQ=255)gagaGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCg > 1:3612611/1‑71 (MQ=255)gagaGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGACGTTCGACGTAGCTCTGCGTCGCTTCAAGCg > 1:625022/1‑71 (MQ=255) agagGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGt > 1:3955954/1‑71 (MQ=255) agagGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGt > 1:1975976/1‑71 (MQ=255) gagGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGtt > 1:2975369/1‑71 (MQ=255) agGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTc > 1:1294462/1‑71 (MQ=255) acaTGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGc < 1:1018230/71‑1 (MQ=255) acaTGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGc < 1:932962/71‑1 (MQ=255) aTGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGa < 1:4697634/71‑1 (MQ=255) aTGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGa < 1:2514086/71‑1 (MQ=255) aTGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGa < 1:4650931/71‑1 (MQ=255) aTGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGa < 1:1768505/71‑1 (MQ=255) tGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGa > 1:542875/1‑70 (MQ=255) gCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGaa > 1:281357/1‑70 (MQ=255) ccGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGaaaa < 1:2203690/71‑1 (MQ=255) ccGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGaaa < 1:2549376/70‑1 (MQ=255) ccGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGaaa > 1:1994760/1‑70 (MQ=255) ccGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGaaa < 1:622552/70‑1 (MQ=255) cGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGaaa > 1:720970/1‑69 (MQ=255) cGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGaaa > 1:2831723/1‑69 (MQ=255) ttAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAgg < 1:1864572/70‑1 (MQ=255) ttAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGgt > 1:711071/1‑71 (MQ=255) tAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGgtg > 1:1850508/1‑71 (MQ=255) tAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGgtg > 1:307214/1‑71 (MQ=255) aaaGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGgtgt > 1:364969/1‑71 (MQ=255) aaaGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGgtgt > 1:4416356/1‑71 (MQ=255) aaaGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGgtgt > 1:3040003/1‑71 (MQ=255) aaGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGgtgt < 1:2317958/70‑1 (MQ=255) cGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGc > 1:127102/1‑59 (MQ=255) cGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGc > 1:2404828/1‑59 (MQ=255) cGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTgg < 1:1992416/70‑1 (MQ=255) tGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGgtgt < 1:2518852/63‑1 (MQ=255) tGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGgtgt < 1:1463528/63‑1 (MQ=255) tGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGgtgt < 1:880993/63‑1 (MQ=255) gAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCgg > 1:2811013/1‑70 (MQ=255) aaaaCGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGgtgt < 1:2022568/61‑1 (MQ=255) aaaaCGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCgg > 1:285253/1‑69 (MQ=255) aaaaCGAGCCGTTCGAAGTCGCAATGCGTCGCTTCAAACGCACCATCGAAAAAACTGGTCTGCTGACGGaa > 1:1449614/1‑71 (MQ=255) aaaaCGAGCCGTTCGAAGTCGCAATGCGTCGCTTCAAACGCACCATCGAAAAAACTGGTCTGCTGACGGaa > 1:1019933/1‑71 (MQ=255) aaaaCGAGCCGTTCGAAGTCGCAATGCGTCGCTTCAAACGCACCATCGAAAAAACTGGTCTGCTGACGGa > 1:432435/1‑70 (MQ=255) aaaCGAGCCGTTCGAAGTCGCAATGCGTCGCTTCAAACGCACCATCGAAAAAACTGGTCTGCTGACGGaac < 1:3206717/71‑2 (MQ=255) aaaCGAGCCGTTCGAAGTCGCAATGCGTCGCTTCAAACGCACCATCGAAAAAACTGGTCTGCTGACGGaac < 1:1410787/71‑2 (MQ=255) aaCGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGt < 1:987020/71‑1 (MQ=255) aaCGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGt < 1:4660032/71‑1 (MQ=255) aaCGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGt < 1:4741940/71‑1 (MQ=255) aaCGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGt > 1:283093/1‑71 (MQ=255) aGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGaaaaa > 1:217485/1‑47 (MQ=255) aGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGaaaaa > 1:4322648/1‑47 (MQ=255) gTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTtcgtcgtc < 1:1336043/71‑1 (MQ=255) gTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTtcgtcgtc < 1:1479754/71‑1 (MQ=255) ttCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGaa < 1:4548268/39‑1 (MQ=255) cGACGTAGCTCTGCGTCGCTTCAAGCTTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTcgtcgtcgt > 1:4333830/1‑70 (MQ=255) cGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTc > 1:375935/1‑50 (MQ=255) cGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGaa > 1:544467/1‑58 (MQ=255) cGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGaa > 1:353370/1‑58 (MQ=255) cGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGaa > 1:2890048/1‑58 (MQ=255) cGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGaa > 1:946537/1‑58 (MQ=255) cGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTcgtcgtcgt > 1:2437457/1‑70 (MQ=255) cGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTcgtcgtcgt < 1:3036209/70‑1 (MQ=255) gACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTg < 1:4286524/70‑1 (MQ=255) tAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGtt < 1:2920429/44‑1 (MQ=255) aGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGt > 1:3257521/1‑68 (MQ=255) aGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTc > 1:1869581/1‑70 (MQ=255) gCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCg < 1:3799372/48‑1 (MQ=255) gCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTa < 1:1819439/71‑1 (MQ=255) gCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGAGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCt < 1:4684452/70‑1 (MQ=255) tctGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTAt < 1:718194/70‑1 (MQ=255) ctGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTtcgtcgtc < 1:2316137/58‑1 (MQ=255) ctGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTtcgtcgtc < 1:1307541/58‑1 (MQ=255) ctGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTtcgtcgtc < 1:1663780/58‑1 (MQ=255) ctGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTtcgtcgtc < 1:173323/58‑1 (MQ=255) ctGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTtcgtcgtc < 1:867195/58‑1 (MQ=255) cGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGaaa > 1:2984343/1‑70 (MQ=255) gCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTcgtcgtcgt > 1:2857210/1‑53 (MQ=255) gCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTTGTGAGTTCTATGAAAAACCg < 1:4737592/71‑1 (MQ=255) gCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGaaa > 1:4551479/1‑66 (MQ=255) gCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGaaa > 1:735017/1‑66 (MQ=255) gCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGaaa > 1:1500118/1‑66 (MQ=255) gCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGaaa > 1:4228349/1‑66 (MQ=255) gCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGaaa > 1:3080315/1‑66 (MQ=255) gCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGCAAAAcc > 1:102703/1‑70 (MQ=255) ttCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGAAAAAcc < 1:1057783/68‑1 (MQ=255) cAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGAAAAACCGACTa > 1:422836/1‑71 (MQ=255) cAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGAAAAACCGACTa > 1:1024470/1‑71 (MQ=255) aGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGAAAAAc > 1:4381512/1‑63 (MQ=255) aGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGAAAAACCGACTAcc > 1:1270394/1‑71 (MQ=255) gCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGAAAAACCGACTACCg < 1:926129/71‑1 (MQ=255) | GAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGAAAAACCGACTACCG > minE/2061485‑2061623 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |