Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,960,739 Δ1 bp 10.6% intergenic (+189/+231) yjjV → / ← yjjW predicted DNase/predicted pyruvate formate lyase activating enzyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,960,7390G.10.6% 145.4 / 9.6 47intergenic (+189/+231)yjjV/yjjWpredicted DNase/predicted pyruvate formate lyase activating enzyme
Reads supporting (aligned to +/- strand):  ref base G (14/28);  new base . (3/2);  total (17/30)
Fisher's exact test for biased strand distribution p-value = 3.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.33e-01

CGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGAC  >  minE/2960678‑2960808
                                                             |                                                                     
cGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGcc                                                                   >  1:729297/1‑66 (MQ=40)
 ggCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCg                                                             >  1:1519038/1‑71 (MQ=39)
  tcAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGa                                                                 <  1:2250453/65‑1 (MQ=255)
   cAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTa                                                           <  1:1158872/71‑1 (MQ=39)
   cAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTa                                                           <  1:2366611/71‑1 (MQ=39)
      ttGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCtg                                                                       >  1:3603993/1‑56 (MQ=255)
        ggCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGcc                                                       <  1:522082/70‑1 (MQ=255)
         gcgcACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGcctg                                                     <  1:2138097/71‑3 (MQ=255)
          cgcACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGa                                                    <  1:3105339/71‑1 (MQ=255)
           gcACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCg                                                                  <  1:87461/56‑1 (MQ=255)
           gcACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCg                                                                  <  1:2178458/56‑1 (MQ=255)
           gcACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCg                                                                  <  1:2353149/56‑1 (MQ=255)
           gcACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGAt                                                   <  1:1050671/71‑1 (MQ=255)
            cacaATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATa                                                  <  1:2414918/71‑1 (MQ=255)
              caATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCGGATAAgg                                               >  1:1629148/1‑70 (MQ=11)
               aaTGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCtgt                                                                      <  1:3706744/48‑1 (MQ=255)
                aTGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGc                                              <  1:549425/71‑1 (MQ=39)
                aTGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGc                                              <  1:2031189/71‑1 (MQ=39)
                aTGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGcac                                            >  1:1028695/1‑69 (MQ=2)
                 tGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGcact                                           >  1:619685/1‑68 (MQ=0)
                 tGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCgc                                            <  1:636913/70‑2 (MQ=11)
                   ccTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGtt                                           <  1:2200443/71‑1 (MQ=39)
                   ccTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGtt                                           <  1:227800/71‑1 (MQ=39)
                    cTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTc                                          <  1:1549748/71‑1 (MQ=39)
                    cTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTc                                          <  1:3634524/71‑1 (MQ=39)
                    cTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTc                                          <  1:2483357/71‑1 (MQ=39)
                    cTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTc                                          <  1:422685/71‑1 (MQ=39)
                         gCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCAcgc                                      >  1:1879315/1‑70 (MQ=255)
                           gACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCccgccg                                    >  1:3668052/1‑70 (MQ=255)
                              gCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAAc                                                               >  1:491565/1‑40 (MQ=255)
                              gCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATc                                >  1:204965/1‑71 (MQ=255)
                                ttGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGc                                                        >  1:3877196/1‑45 (MQ=255)
                                ttGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCg                              >  1:1713907/1‑71 (MQ=255)
                                ttGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCg                              >  1:1175304/1‑71 (MQ=255)
                                ctGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATa                                                  <  1:1403060/49‑1 (MQ=2)
                                   tCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCa                           <  1:1050223/71‑1 (MQ=40)
                                       gTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCg                       >  1:3188691/1‑71 (MQ=255)
                                            aTCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCgg                             <  1:3010247/60‑1 (MQ=255)
                                            aTCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCgg                             <  1:1795392/60‑1 (MQ=255)
                                            aTCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCgg                             <  1:1270326/60‑1 (MQ=255)
                                            aTCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCa                  >  1:887940/1‑71 (MQ=255)
                                              cATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGtt                                           >  1:2011826/1‑44 (MQ=255)
                                              cATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTc                        >  1:2168649/1‑63 (MQ=255)
                                               aTGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCgg                             <  1:3715563/57‑1 (MQ=255)
                                                    tACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGc                                              <  1:949416/35‑1 (MQ=255)
                                                     aCAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCt          >  1:673345/1‑70 (MQ=255)
                                                      cAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCt          <  1:1884132/69‑1 (MQ=255)
                                                      cAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGa        <  1:1546678/71‑1 (MQ=255)
                                                      cAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGa        <  1:1265104/71‑1 (MQ=255)
                                                            tgtgCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGAc  >  1:3556926/1‑71 (MQ=39)
                                                            tgtgCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGAc  >  1:2020011/1‑71 (MQ=39)
                                                             |                                                                     
CGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGAC  >  minE/2960678‑2960808

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: