Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	608,693	Δ1 bp	10.3%	coding (1561/2283 nt)	pflB ←	pyruvate formate lyase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	608,693	0	G	.	10.3%	243.2 / 18.7	78	coding (1561/2283 nt)	pflB	pyruvate formate lyase I
Reads supporting (aligned to +/- strand):  ref base G (34/36);  new base . (4/4);  total (38/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.00e-01

TCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATG  >  minE/608624‑608763                                                                      |                                                                        gggCATATTTGATGGCAGACAGGGAGTCCGTCGCCACCGACAGGCCCGCGATGCCGCATGCCATAGTGCga                                                                        <  1:97846/69‑2 (MQ=255)     atatTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCg                                                                         >  1:3663084/1‑66 (MQ=255)     atatTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCg                                                                         <  1:1283174/66‑1 (MQ=255)        tttGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGAGGATa‑a                                                                   >  1:131469/1‑68 (MQ=255)         ttGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACg                                                                 <  1:549217/69‑1 (MQ=255)           gATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATa‑a                                                                   >  1:3247054/1‑65 (MQ=255)           gATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCAc                                                             >  1:880896/1‑71 (MQ=255)            aTTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACg                                                            <  1:1839862/71‑1 (MQ=255)               gCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGa                                                                       >  1:517001/1‑58 (MQ=255)               gCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGt                                                          <  1:882219/70‑1 (MQ=255)               gCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTc                                                         >  1:3093960/1‑71 (MQ=255)                  gACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTg                                                      >  1:989717/1‑71 (MQ=255)                   aCAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGt                                                                >  1:3476678/1‑60 (MQ=255)                   aCAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTg                                                      >  1:1112320/1‑70 (MQ=255)                   aCAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTg                                                      >  1:827740/1‑70 (MQ=255)                   aCAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTg                                                      >  1:3782036/1‑70 (MQ=255)                       ggAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAgcg                                                 >  1:2005090/1‑71 (MQ=255)                       ggAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAgcg                                                 <  1:3705642/71‑1 (MQ=255)                        gAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCg                                                                         <  1:3402155/47‑1 (MQ=255)                         aGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATa                                                                     <  1:1920507/50‑1 (MQ=255)                          gTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCa                                              <  1:3249682/71‑1 (MQ=255)                          gTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCa                                              <  1:2950475/71‑1 (MQ=255)                           tCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑Ac                                                                  >  1:3074245/1‑50 (MQ=255)                           tCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑Ac                                                                  >  1:3078752/1‑50 (MQ=255)                           tCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCa                                              <  1:1277/70‑1 (MQ=255)                           tCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCAt                                             <  1:609911/71‑1 (MQ=255)                           tCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCAt                                             >  1:3428425/1‑71 (MQ=255)                             agcagcAATGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCa                                           <  1:98677/71‑1 (MQ=255)                             agcagcAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑Ac                                                                  <  1:2761886/48‑1 (MQ=255)                             agcagcAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCa                                           <  1:2589152/71‑1 (MQ=255)                               cagcaACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAgcg                                                 <  1:3737942/63‑1 (MQ=255)                               cagcaACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCaga                                         <  1:1218174/71‑1 (MQ=255)                                agcaACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGcc                                               <  1:895464/64‑1 (MQ=255)                                agcaACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCagag                                        <  1:3678985/71‑1 (MQ=255)                                 gcaACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCa                                           >  1:220826/1‑67 (MQ=255)                                   aaCGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCa                                                    <  1:2887690/56‑1 (MQ=255)                                     ccgACAGGCCCGCGATGCCGCATGCCATAGTGC‑GATAGACATCACGATCGTGCAGCGCCATCAGCGAAGCt                                   <  1:1898037/69‑1 (MQ=255)                                     cGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCt                                   >  1:3421578/1‑71 (MQ=255)                                      ggACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCag                                          >  1:1146727/1‑63 (MQ=255)                                      cgACAGGCCCGCGATGCCGCATGCCATAGTGC‑GATAGACATCACGATCGTGCAGCGCCATCAGCGAAGCtt                                  >  1:150386/2‑71 (MQ=255)                                       gACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTg                                                      >  1:1831780/1‑50 (MQ=255)                                        aCAGGCCCGCGATGCCGCATGCCATAGTGC‑GATAGACATCACGATCGTGCAGCGCCATCAGCGAAGCTTCg                                >  1:943171/1‑71 (MQ=255)                                        aCAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCg                                >  1:1107471/1‑71 (MQ=255)                                        aCAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCg                                >  1:3799173/1‑71 (MQ=255)                                        aCAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCg                                >  1:3372418/1‑71 (MQ=255)                                         cAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCg                                >  1:3149032/1‑70 (MQ=255)                                         cAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGt                               <  1:3694058/71‑1 (MQ=255)                                          aGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCg                                >  1:96270/1‑69 (MQ=255)                                           gACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCGGAGAGGCTTCGTa                              <  1:215811/70‑1 (MQ=255)                                           gACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTa                              >  1:3193277/1‑70 (MQ=255)                                            aCCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGc                            <  1:1685785/71‑1 (MQ=255)                                              cAGCGATACCACACGCCATGGTGCGGATA‑ACGTCa                                                              >  1:25645/1‑35 (MQ=255)                                              cAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACgg                                                           <  1:2526365/38‑1 (MQ=255)                                              cAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCa                                                    <  1:2533402/45‑1 (MQ=255)                                                gCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGt                         >  1:2710663/1‑70 (MQ=255)                                                gCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTa                        >  1:194067/1‑71 (MQ=255)                                                gCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTa                        >  1:1630226/1‑71 (MQ=255)                                                 cGATGCCGCATGCCATAGTGC‑GATAGACATCACGATCGTGCAGCGCCATCAGCGAAGCTTCGTAGCTGTAc                       <  1:2629928/71‑1 (MQ=255)                                                  gATGCCGCATGCCATAGTGC‑GATAGACATCACGATCGTGCAGCGCCATCAGCGAAGCTTCGTAGCTGTAc                       <  1:3079912/70‑1 (MQ=255)                                                   aTACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTg                          <  1:1217645/66‑1 (MQ=255)                                                   aTACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACtt                     <  1:1694886/71‑1 (MQ=255)                                                    tACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTg                    >  1:1711142/1‑71 (MQ=255)                                                     aCCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGt                   >  1:1712925/1‑71 (MQ=255)                                                     aCCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGt                   >  1:411857/1‑71 (MQ=255)                                                      ccACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTc                  <  1:3614236/71‑1 (MQ=255)                                                       cacaCGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCg                 <  1:256898/71‑1 (MQ=255)                                                         catgCCATAGTGC‑GATAGACATCACGATCGTGCAGCGCCATCAGCGAAGCTTCGTAGCTGTACTTGTCGt                <  1:1649123/67‑1 (MQ=255)                                                         cacGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGt                   >  1:1554954/1‑67 (MQ=255)                                                           tgCCATAGTGC‑GATAGACATCACGATCGTGCAGCGCCATCAGCGAAGCTTCGTAGCTGTACTTGTCGTGCa             >  1:2448498/2‑71 (MQ=255)                                                           tgCCATAGTGC‑GATAGACATCACGATCGTGCAGCGCCATCAGCGAAGCTTCGTAGCTGTACTTGTCGTGCa             >  1:514557/2‑71 (MQ=255)                                                           cGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTa                        <  1:1576949/60‑1 (MQ=255)                                                               aTGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATg           >  1:3881228/1‑69 (MQ=255)                                                               aTGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTa         <  1:1979514/71‑1 (MQ=255)                                                               aTGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTa         <  1:448813/71‑1 (MQ=255)                                                                  gTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGt       <  1:1766988/70‑1 (MQ=255)                                                                  gTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTg      <  1:2598374/71‑1 (MQ=255)                                                                   tGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTgg     <  1:693798/71‑1 (MQ=255)                                                                   tGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTgg     <  1:3700027/71‑1 (MQ=255)                                                                     cGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTc                                 >  1:617567/1‑41 (MQ=255)                                                                     cGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCTTGCATGTAGTGga    <  1:180471/70‑1 (MQ=255)                                                                     cGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGga    <  1:3045120/70‑1 (MQ=255)                                                                     cGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGga    <  1:115108/70‑1 (MQ=255)                                                                      ggATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGgatg  <  1:1051507/71‑1 (MQ=255)                                                                      ggATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGgat   >  1:2498510/1‑70 (MQ=255)                                                                      ggATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGgat   >  1:1712621/1‑70 (MQ=255)                                                                      |                                                                        TCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATG  >  minE/608624‑608763

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑