| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,467,910:2 | +T | 25.9% | intergenic (+69/+59) | ubiG → / ← yfaL | bifunctional 3‑demethylubiquinone‑9 3‑methyltransferase and 2‑octaprenyl‑6‑hydroxy phenol methylase/adhesin |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,467,910 | 2 | . | T | 25.9% | 158.6 / 67.4 | 54 | intergenic (+69/+59) | ubiG/yfaL | bifunctional 3‑demethylubiquinone‑9 3‑methyltransferase and 2‑octaprenyl‑6‑hydroxy phenol methylase/adhesin |
| Reads supporting (aligned to +/- strand): ref base . (19/21); new base T (8/6); total (27/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.57e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTAC‑AAAT‑‑‑‑G‑‑CTCC‑‑CCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTT > minE/1467845‑1467977 | tGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTAC‑AAAT‑‑‑‑G‑‑CTCC‑‑CCg > 1:1061835/1‑69 (MQ=255) gATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTAC‑AAAT‑‑‑‑G‑‑CTCC‑‑CCGTAg > 1:1395669/1‑71 (MQ=255) aTGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTAC‑AAAT‑‑‑‑G‑‑CTCC‑‑CCGTAg < 1:3460347/70‑1 (MQ=255) ttGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTAC‑AAAT‑‑‑‑G‑‑CTCC‑‑CCGTAGGCCg > 1:21774/1‑70 (MQ=255) gCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTAC‑AAAT‑‑‑‑G‑‑CTCC‑‑CCGTAGGCCGGATa < 1:959386/71‑1 (MQ=255) gCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTAC‑AAAT‑‑‑‑G‑‑CTCC‑‑CCGTAGGCCGGATa < 1:2080018/71‑1 (MQ=255) cgcCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTAC‑AAAT‑‑‑‑G‑‑CTCC‑‑CCGTAGGCCGGATAAGGc < 1:2404202/71‑1 (MQ=255) ggttGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAAC‑‑‑‑GCACTAC‑‑CCGTAGGTCGGATAAGGc > 1:3763323/4‑71 (MQ=2) cAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTAC‑AAAT‑‑‑‑G‑‑CTCC‑‑CCGTAGGCCTGATAAGGCGtt < 1:105602/71‑1 (MQ=255) aaTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTAC‑AAAT‑‑‑‑G‑‑CTCC‑‑CCGTAGGCCGGATAAGGCGttt < 1:3945874/71‑1 (MQ=255) aaTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTAC‑AAAT‑‑‑‑G‑‑CTCC‑‑CCGTAGGCCGGATAAGGCGtt > 1:785002/1‑70 (MQ=255) aTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTAC‑AAAT‑‑‑‑G‑‑CTCC‑‑CCGTAGGCCGGATAAGGCGTTTa > 1:552824/1‑71 (MQ=255) tGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAAC‑‑‑‑GCACTAC‑‑CCGTAGGTCGGATAAGGCGt > 1:155206/1‑70 (MQ=11) tGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTAC‑AAAT‑‑‑‑G‑‑CTCC‑‑CCGTAGGCCGGATAAGGCGTTTa < 1:3159968/70‑1 (MQ=255) gCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTAC‑AAAT‑‑‑‑G‑‑CTCC‑‑CCGTAGGCCGGATAAGGCGTTTAcg > 1:1053630/1‑71 (MQ=255) ccTGATGCGACGCTTGCCGCGTCTTATCAGGCCTAC‑AAAT‑‑‑‑G‑‑CTCC‑‑CCGTAGGCCGGATAAGGCGTTTAcg < 1:58221/70‑1 (MQ=255) gATGCGACGCTTGCCGCGTCTTATCAGGCCTAC‑AAAT‑‑‑‑G‑‑CTCC‑‑CCGTAGGCCGGAt > 1:1678345/1‑55 (MQ=255) gATGCGACGCTTGCCGCGTCTTATCAGGCCTAC‑AAAT‑‑‑‑G‑‑CTCC‑‑CCGTAGGCCGGAt > 1:2093085/1‑55 (MQ=255) gATGCGACGCTTG‑CGCGTCTTATCAGGCCTAC‑AAATTCCCG‑‑CACCCTCCGTAGGCCGGATAAGGCGTTTAc > 1:3139993/1‑71 (MQ=0) aTGCGACGCTTGCCGCGTCTTATCAGGCCTAC‑AAAT‑‑‑‑G‑‑CTCC‑‑CCGTAGGCCGGATAAGGCGTTTACGCCGCa > 1:3890194/1‑71 (MQ=255) aTGCGACGCTTG‑CGCGTCTTATCAGGCCTAC‑AAATTCCCG‑‑CACCCTCCGTAGGCCGGATAAGGCGTTTAcg < 1:3152114/71‑1 (MQ=0) aTGCGACGCTTG‑CGCGTCTTATCAGGCCTAC‑AAATTCCCG‑‑CACCCTCCGTAGGCCGGATAAGGCGTTTAcg < 1:2865534/71‑1 (MQ=0) cGACGCTTGCCGCGTCTTATCAGGCCTAC‑AAAT‑‑‑‑G‑‑CTCC‑‑CCGTAGGCCGGATAAGGCGTTTACGCCGCATc < 1:3065057/70‑1 (MQ=255) cGACGCTTG‑CGCGTCTTATCAGGCCTAC‑AAATTCCCG‑‑CACCCTCCGTAGGCCGGATAAGGCGTTTAcgccg < 1:1147494/71‑1 (MQ=0) gACGCTTG‑CGCGTCTTATCAGGCCTAC‑AAATTCCCG‑‑CACCCTCCGTAGGCCGGATAAGGCGTTTAcgccg > 1:110259/1‑70 (MQ=0) aCGCTTG‑CGCGTCTTATCAGGCCTAC‑AAATTCCCG‑‑CACCCTCCGTAGGCCGGATAAGGCGTTTACGCCGCa < 1:651183/71‑1 (MQ=0) aCGCTTG‑CGCGTCTTATCAGGCCTAC‑AAATTCCCG‑‑CACCCTCCGTAGGCCGGATAAGGCGTTTACGCCGCa > 1:1521629/1‑71 (MQ=0) cGCTTG‑CGCGTCTTATCAGGCCTAC‑AAATTCCCG‑‑CACCCTCCGTAGGCCGGATAAGGCGTTTACGCCGCa > 1:725712/1‑70 (MQ=0) gCTTG‑CGCGTCTTATCAGGCCTAC‑AAATTCCCG‑‑CACCCTCCGTAGGCCGGATAAGGCGTTTACGCCGCATc > 1:2562409/1‑71 (MQ=0) gCTTG‑CGCGTCTTATCAGGCCTAC‑AAATTCCCG‑‑CACCCTCCGTAGGCCGGATAAGGCGTTTACGCCGCATc > 1:2531117/1‑71 (MQ=0) gCTTG‑CGCGTCTTATCAGGCCTAC‑AAATTCCCG‑‑CACCCTCCGTAGGCCGGATAAGGCGTTTACGCCGCATc > 1:3740559/1‑71 (MQ=0) cTTGCCGCGTCTTATCTGGCCTAC‑AAAT‑‑‑‑G‑‑CTCC‑‑CCg < 1:3155041/36‑1 (MQ=255) gCCGCGTCTTATCAGGCCTAC‑AAAT‑‑‑‑G‑‑CTCC‑‑CCGTAg > 1:3487816/1‑36 (MQ=255) acgcgTCTTATCAGGCCTAC‑AAATTCCCG‑‑CGCCATCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGc < 1:2864009/70‑1 (MQ=0) cgcgTCTTATCAGGCCTAC‑AAATTCCCG‑‑CACCCTCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGc > 1:1977681/1‑70 (MQ=2) cgcgTCTTATCAGGCCTAC‑AAATTCCCG‑‑CACCATCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCa < 1:2418182/71‑1 (MQ=2) gcgTCTTATCAGGCCTAC‑AAAT‑‑‑‑G‑‑CTCC‑‑CCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGc < 1:400610/71‑1 (MQ=255) tATCAGGCCTAC‑AAAT‑‑‑‑G‑‑CTCC‑‑CCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGAc > 1:3449640/1‑69 (MQ=255) aTCAGGCCTAC‑AAAT‑‑‑‑G‑‑CTCC‑‑CCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTa < 1:486145/70‑1 (MQ=255) cAGGCCTAC‑AAAT‑‑‑‑G‑‑CTCC‑‑CCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGcc > 1:916471/1‑63 (MQ=255) ggCCTAC‑AAAT‑‑‑‑G‑‑CTCC‑‑CCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAg < 1:3686530/71‑1 (MQ=255) gCCTAC‑AAAT‑‑‑‑G‑‑CTCC‑‑CCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGt > 1:3221245/1‑71 (MQ=255) gCCTAC‑AAAT‑‑‑‑G‑‑CTCC‑‑CCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGt > 1:3476971/1‑71 (MQ=255) ccTAC‑AAAT‑‑‑‑G‑‑CTCC‑‑CCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTg < 1:3586403/71‑1 (MQ=255) cTAC‑AAAT‑‑‑‑G‑‑CTCC‑‑CCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGa < 1:2792831/71‑1 (MQ=255) c‑AAAT‑‑‑‑G‑‑CTCC‑‑CCGTAGGCCGGATAAGGCGTTTAcg > 1:495872/1‑35 (MQ=255) c‑AAAT‑‑‑‑G‑‑CTCC‑‑CCGTAGGCCGGATAAGGCGTTTACGCCGCATCCgg > 1:610608/1‑45 (MQ=255) c‑AAAT‑‑‑‑G‑‑CTCC‑‑CCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGa < 1:2996247/68‑1 (MQ=255) c‑AAAT‑‑‑‑G‑‑CTCC‑‑CCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGa < 1:2453992/68‑1 (MQ=255) c‑AAAT‑‑‑‑G‑‑CTCC‑‑CCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGAt < 1:2304244/69‑1 (MQ=255) c‑AAAT‑‑‑‑G‑‑CTCC‑‑CCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGAt < 1:1145492/69‑1 (MQ=255) aaaT‑‑‑‑G‑‑CTCC‑‑CCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTAc < 1:3428344/71‑1 (MQ=255) aaaT‑‑‑‑G‑‑CTCC‑‑CCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTAc > 1:437211/1‑71 (MQ=255) tCC‑‑CCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGAt < 1:1516659/62‑1 (MQ=255) cc‑‑ccGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCAttt < 1:2240166/69‑1 (MQ=255) | TGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTAC‑AAAT‑‑‑‑G‑‑CTCC‑‑CCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTT > minE/1467845‑1467977 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |