| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,823,325:1 | +G | 23.7% | intergenic (+62/+66) | norW → / ← hypF | NADH:flavorubredoxin oxidoreductase/carbamoyl phosphate phosphatase and maturation protein for [NiFe] hydrogenases |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,823,325 | 1 | . | G | 23.7% | 95.6 / 42.9 | 38 | intergenic (+62/+66) | norW/hypF | NADH:flavorubredoxin oxidoreductase/carbamoyl phosphate phosphatase and maturation protein for [NiFe] hydrogenases |
| Reads supporting (aligned to +/- strand): ref base . (9/20); new base G (2/7); total (11/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.37e-01 | |||||||||||
ATGTAGGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGA‑CGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGTCCAAAT > minE/1823258‑1823380 | aTGTAGGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGA‑CGc < 1:2478489/71‑1 (MQ=255) tGTAGGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGA‑CGCa > 1:2126560/1‑71 (MQ=255) ggCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGA‑CGCATGTGTAgg < 1:3860173/71‑1 (MQ=255) ggCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGA‑CGCATGTGTAgg < 1:1731577/71‑1 (MQ=255) tACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGA‑CGCATGTGTAGGCCg > 1:1062286/1‑71 (MQ=255) cTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGA‑CGCATGTGTAGGCCGGa < 1:2140307/71‑1 (MQ=255) tgCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGA‑CGCATGTGTAGGCCGGATa < 1:850126/70‑1 (MQ=255) tgCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGA‑CGCATGTGTAGGCCGGATa < 1:1366859/70‑1 (MQ=255) tgCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGA‑CGCATGTGTAGGCCGGATa < 1:2470456/70‑1 (MQ=255) ccTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGA‑CGCATGTGTAGGCCGGATAAgg < 1:1193108/71‑1 (MQ=255) aaaTGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGA‑CGCATGTGTAGGCCGGATAAGGCGtt > 1:523859/1‑71 (MQ=255) gtGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGttt < 1:1518391/70‑1 (MQ=21) tGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGA‑CGCATGTGTAGGCCGGATAAGGCGTTTAc < 1:1564648/71‑1 (MQ=255) tCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGA‑CGCAtg < 1:77009/46‑1 (MQ=255) tCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGA‑CGCATGTGTAGGCCGGATAAGGCGTTTAcg < 1:3682640/70‑1 (MQ=255) tCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGAt < 1:3729480/59‑1 (MQ=18) tCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTAcg < 1:3630201/71‑1 (MQ=21) ggATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGA‑Cg > 1:3024933/1‑40 (MQ=255) gATTCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCAtttg < 1:2979983/46‑1 (MQ=11) gATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCAtttg < 1:1020986/46‑1 (MQ=11) tGCGACGCTGGCGCGTCTTATCCGACCTACGGGGA‑CGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCAt < 1:2192944/71‑1 (MQ=255) tGCGACGCTGGCGCGTCTTATCCGACCTACGGGGA‑CGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCAt > 1:2065098/1‑71 (MQ=255) cGCTGGCGCGTCTTATCCGACCTACGGGGA‑CGCATGTGTAgg > 1:3869039/1‑42 (MQ=255) cGCTGGCGCGTCTTATCCGACCTACGGGGA‑CGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGc < 1:2743751/71‑1 (MQ=255) cGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATc > 1:3271078/1‑68 (MQ=18) gCTGGCGCGTCTTATCCGACCTACGGGGA‑CGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCa < 1:2999668/71‑1 (MQ=255) gacgcgTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCac > 1:2978610/3‑69 (MQ=18) acgcgTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCacc < 1:3187666/69‑3 (MQ=18) cgTCTTATCCGACCTACGGGGA‑CGCATGTGTAGGCCGGATa > 1:3005508/1‑41 (MQ=255) tCTTATCCGACCTACGGGGA‑CGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCaa < 1:1946645/63‑1 (MQ=255) aTCCGACCTACGGGGA‑CGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGgt < 1:3298407/63‑1 (MQ=255) aTCCGACCTACGGGGA‑CGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGTCCaa < 1:2020963/69‑1 (MQ=255) ccGACCTACGGGGA‑CGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGgtgt > 1:1546459/1‑63 (MQ=255) aCCTACGGGGA‑CGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGTCCAAAt < 1:3934155/66‑1 (MQ=255) aCCTACGGGGA‑CGCATGTGGAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGTCCAAAt < 1:863585/66‑1 (MQ=255) tACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTAc < 1:3781593/38‑1 (MQ=14) aCGGGGA‑CGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCa < 1:3070990/42‑1 (MQ=255) aCGGGGA‑CGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGTCCaaa > 1:3220937/1‑61 (MQ=255) | ATGTAGGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGA‑CGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGTCCAAAT > minE/1823258‑1823380 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |