Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,571,344:1 +A 16.9% intergenic (+112/‑1667) yibH → / → ysaB hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,571,3441.A16.9% 167.7 / 44.2 59intergenic (+112/‑1667)yibH/ysaBhypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (18/31);  new base A (5/5);  total (23/36)
Fisher's exact test for biased strand distribution p-value = 4.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

ATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCG  >  minE/2571277‑2571411
                                                                     |                                                                   
attattTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTg                                                                   <  1:2119564/70‑1 (MQ=255)
 ttattTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGa                                                                 <  1:1232140/71‑1 (MQ=255)
 ttattTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGa                                                                 <  1:2225942/71‑1 (MQ=255)
  tattTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGAt                                                                >  1:85200/1‑71 (MQ=255)
  tattTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGAt                                                                >  1:2182597/1‑71 (MQ=255)
  tattTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGAt                                                                >  1:418124/1‑71 (MQ=255)
   attTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGAt                                                                >  1:2254309/1‑70 (MQ=255)
    tttttGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATg                                                               <  1:3533469/70‑1 (MQ=255)
      tttGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAg                                                             <  1:2502571/70‑1 (MQ=255)
        tGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAgtg                                                           <  1:2389877/70‑1 (MQ=255)
         gCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAgtgt                                                          >  1:1360047/1‑70 (MQ=255)
           atatTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTAct                                                       <  1:2694549/71‑1 (MQ=255)
              ttACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGctct                                                     <  1:175902/70‑1 (MQ=255)
                aCTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCa                                                   <  1:3027968/70‑1 (MQ=255)
                aCTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCa                                                   <  1:1985376/70‑1 (MQ=255)
                 cTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCAtt                                                 <  1:2485622/71‑1 (MQ=255)
                    cATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGa                                                                 >  1:1637292/1‑52 (MQ=255)
                    cATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTg                                                <  1:2992931/69‑1 (MQ=255)
                    cATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGa                                              >  1:2008979/1‑71 (MQ=255)
                     aTTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGGGTGCTCTCATTGGAt                                             <  1:2791507/71‑1 (MQ=255)
                      ttAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTg                                                                   <  1:909931/48‑1 (MQ=255)
                      ttAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTg                                                                   <  1:1884285/48‑1 (MQ=255)
                        aGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGa                                                                 >  1:2105731/1‑48 (MQ=255)
                          cTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGAtttttt                                        <  1:51191/71‑1 (MQ=255)
                          cTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGAtttttt                                        <  1:1809107/71‑1 (MQ=255)
                          cTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGAtttttt                                        <  1:1410050/71‑1 (MQ=255)
                            tCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAgt                                                            >  1:1241951/1‑49 (MQ=255)
                            tCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAgt                                                            >  1:2625243/1‑49 (MQ=255)
                            gtgatgatTTTTCCTTTAATATCCAGCATGTTAAGAAGTTCt                                                                    >  1:1733264/3‑42 (MQ=12)
                            gtgatgatTTTTCCTTTAATATCCAGCATGTTAAGAAGTTCTg                                                                   >  1:2571275/3‑43 (MQ=16)
                             cGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATc                                     >  1:466854/1‑71 (MQ=255)
                              gatgatTTTTCCTTTAATATCCAGCATGTAAAGAAGTTc                                                                      >  1:2962167/1‑39 (MQ=9)
                               atgatTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCg                                   <  1:1035998/71‑1 (MQ=255)
                                tgatTTTTCCTTTAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATcc                                    >  1:602030/1‑71 (MQ=255)
                                 gatTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTc                                 >  1:334079/1‑71 (MQ=255)
                                  atTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTc                                 <  1:833991/70‑1 (MQ=255)
                                       tccTTTAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTc                                 >  1:1663272/3‑67 (MQ=255)
                                       tccTTTAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTCTTg                              <  1:1699436/68‑1 (MQ=255)
                                        ccTTTAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTCTTga                             <  1:2002593/69‑2 (MQ=255)
                                         cTTTAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTACCCGTCTTGATc                           <  1:681628/71‑1 (MQ=255)
                                         atttAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTCTTGATc                           >  1:1204721/2‑71 (MQ=255)
                                           ttAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTCTTGATCTg                         >  1:1385251/1‑71 (MQ=255)
                                            tAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTCTTGATCTgt                        >  1:3859165/1‑70 (MQ=255)
                                               tatTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGAt                                             <  1:2954658/46‑1 (MQ=255)
                                               tatTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTAACCGTCTTGTTCTGCCGc                     <  1:1658843/70‑1 (MQ=255)
                                                    aTTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGAt                <  1:2701468/70‑1 (MQ=255)
                                                    aTTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGAt                <  1:3677991/70‑1 (MQ=255)
                                                     ttATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATga              <  1:2009661/71‑1 (MQ=255)
                                                     gcaTGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTa                                       <  1:3193494/45‑1 (MQ=255)
                                                     gcaTGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTa                                       <  1:3041316/45‑1 (MQ=255)
                                                      tATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTc                           >  1:779794/1‑57 (MQ=255)
                                                      tATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGa                 >  1:2418661/1‑67 (MQ=255)
                                                       aTGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATgag             <  1:3209317/70‑1 (MQ=255)
                                                        tGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGAc           >  1:355609/1‑71 (MQ=255)
                                                          ttAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACt          <  1:209001/70‑1 (MQ=255)
                                                          ttAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACt          <  1:3269259/70‑1 (MQ=255)
                                                            aataaGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACtat        <  1:257301/70‑1 (MQ=255)
                                                             ataaGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACtata       <  1:2815788/70‑1 (MQ=255)
                                                              taaGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACa     >  1:13632/1‑71 (MQ=255)
                                                               aaGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACAt    >  1:3522116/1‑71 (MQ=255)
                                                                 gTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACAtcg  <  1:1944225/71‑1 (MQ=255)
                                                                     |                                                                   
ATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCG  >  minE/2571277‑2571411

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: