Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	608,697:1	+G	10.1%	coding (1557/2283 nt)	pflB ←	pyruvate formate lyase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	608,697	1	.	G	10.1%	248.3 / 34.2	79	coding (1557/2283 nt)	pflB	pyruvate formate lyase I
Reads supporting (aligned to +/- strand):  ref base . (33/38);  new base G (4/4);  total (37/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01

TTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGT  >  minE/608631‑608767                                                                    |                                                                       tttGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGAGGATa‑a                                                                       >  1:131469/1‑68 (MQ=255)  ttGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACg                                                                     <  1:549217/69‑1 (MQ=255)    gATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATa‑a                                                                       >  1:3247054/1‑65 (MQ=255)    gATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCAc                                                                 >  1:880896/1‑71 (MQ=255)     aTTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACg                                                                <  1:1839862/71‑1 (MQ=255)        gCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGt                                                              <  1:882219/70‑1 (MQ=255)        gCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTc                                                             >  1:3093960/1‑71 (MQ=255)           gACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTg                                                          >  1:989717/1‑71 (MQ=255)            aCAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGt                                                                    >  1:3476678/1‑60 (MQ=255)            aCAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTg                                                          >  1:1112320/1‑70 (MQ=255)            aCAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTg                                                          >  1:827740/1‑70 (MQ=255)            aCAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTg                                                          >  1:3782036/1‑70 (MQ=255)                ggAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAgcg                                                     <  1:3705642/71‑1 (MQ=255)                ggAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAgcg                                                     >  1:2005090/1‑71 (MQ=255)                  aGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATa                                                                         <  1:1920507/50‑1 (MQ=255)                   gTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCa                                                  <  1:2950475/71‑1 (MQ=255)                   gTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCa                                                  <  1:3249682/71‑1 (MQ=255)                    tCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑Ac                                                                      >  1:3074245/1‑50 (MQ=255)                    tCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑Ac                                                                      >  1:3078752/1‑50 (MQ=255)                    tCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCa                                                  <  1:1277/70‑1 (MQ=255)                    tCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCAt                                                 >  1:3428425/1‑71 (MQ=255)                    tCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCAt                                                 <  1:609911/71‑1 (MQ=255)                      agcagcAATGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCa                                               <  1:98677/71‑1 (MQ=255)                      agcagcAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑Ac                                                                      <  1:2761886/48‑1 (MQ=255)                      agcagcAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCa                                               <  1:2589152/71‑1 (MQ=255)                        cagcaACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAgcg                                                     <  1:3737942/63‑1 (MQ=255)                        cagcaACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCaga                                             <  1:1218174/71‑1 (MQ=255)                         agcaACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGcc                                                   <  1:895464/64‑1 (MQ=255)                         agcaACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCagag                                            <  1:3678985/71‑1 (MQ=255)                          gcaACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCa                                               >  1:220826/1‑67 (MQ=255)                            aaCGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCa                                                        <  1:2887690/56‑1 (MQ=255)                              ccgACAGGCCCGCGATGCCGCATGCCATAGTGC‑GATAGACATCACGATCGTGCAGCGCCATCAGCGAAGCt                                       <  1:1898037/69‑1 (MQ=255)                              cGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCt                                       >  1:3421578/1‑71 (MQ=255)                               ggACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCag                                              >  1:1146727/1‑63 (MQ=255)                               cgACAGGCCCGCGATGCCGCATGCCATAGTGC‑GATAGACATCACGATCGTGCAGCGCCATCAGCGAAGCtt                                      >  1:150386/2‑71 (MQ=255)                                gACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTg                                                          >  1:1831780/1‑50 (MQ=255)                                 aCAGGCCCGCGATGCCGCATGCCATAGTGC‑GATAGACATCACGATCGTGCAGCGCCATCAGCGAAGCTTCg                                    >  1:943171/1‑71 (MQ=255)                                 aCAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCg                                    >  1:3372418/1‑71 (MQ=255)                                 aCAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCg                                    >  1:3799173/1‑71 (MQ=255)                                 aCAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCg                                    >  1:1107471/1‑71 (MQ=255)                                  cAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCg                                    >  1:3149032/1‑70 (MQ=255)                                  cAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGt                                   <  1:3694058/71‑1 (MQ=255)                                   aGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCg                                    >  1:96270/1‑69 (MQ=255)                                    gACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCGGAGAGGCTTCGTa                                  <  1:215811/70‑1 (MQ=255)                                    gACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTa                                  >  1:3193277/1‑70 (MQ=255)                                     aCCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGc                                <  1:1685785/71‑1 (MQ=255)                                       cAGCGATACCACACGCCATGGTGCGGATA‑ACGTCa                                                                  >  1:25645/1‑35 (MQ=255)                                       cAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACgg                                                               <  1:2526365/38‑1 (MQ=255)                                       cAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCa                                                        <  1:2533402/45‑1 (MQ=255)                                         gCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGt                             >  1:2710663/1‑70 (MQ=255)                                         gCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTa                            >  1:1630226/1‑71 (MQ=255)                                         gCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTa                            >  1:194067/1‑71 (MQ=255)                                          cGATGCCGCATGCCATAGTGC‑GATAGACATCACGATCGTGCAGCGCCATCAGCGAAGCTTCGTAGCTGTAc                           <  1:2629928/71‑1 (MQ=255)                                           gATGCCGCATGCCATAGTGC‑GATAGACATCACGATCGTGCAGCGCCATCAGCGAAGCTTCGTAGCTGTAc                           <  1:3079912/70‑1 (MQ=255)                                            aTACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTg                              <  1:1217645/66‑1 (MQ=255)                                            aTACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACtt                         <  1:1694886/71‑1 (MQ=255)                                             tACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTg                        >  1:1711142/1‑71 (MQ=255)                                              aCCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGt                       >  1:1712925/1‑71 (MQ=255)                                              aCCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGt                       >  1:411857/1‑71 (MQ=255)                                               ccACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTc                      <  1:3614236/71‑1 (MQ=255)                                                cacaCGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCg                     <  1:256898/71‑1 (MQ=255)                                                  catgCCATAGTGC‑GATAGACATCACGATCGTGCAGCGCCATCAGCGAAGCTTCGTAGCTGTACTTGTCGt                    <  1:1649123/67‑1 (MQ=255)                                                  cacGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGt                       >  1:1554954/1‑67 (MQ=255)                                                    tgCCATAGTGC‑GATAGACATCACGATCGTGCAGCGCCATCAGCGAAGCTTCGTAGCTGTACTTGTCGTGCa                 >  1:514557/2‑71 (MQ=255)                                                    tgCCATAGTGC‑GATAGACATCACGATCGTGCAGCGCCATCAGCGAAGCTTCGTAGCTGTACTTGTCGTGCa                 >  1:2448498/2‑71 (MQ=255)                                                    cGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTa                            <  1:1576949/60‑1 (MQ=255)                                                        aTGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATg               >  1:3881228/1‑69 (MQ=255)                                                        aTGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTa             <  1:448813/71‑1 (MQ=255)                                                        aTGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTa             <  1:1979514/71‑1 (MQ=255)                                                           gTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGt           <  1:1766988/70‑1 (MQ=255)                                                           gTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTg          <  1:2598374/71‑1 (MQ=255)                                                            tGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTgg         <  1:3700027/71‑1 (MQ=255)                                                            tGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTgg         <  1:693798/71‑1 (MQ=255)                                                              cGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTc                                     >  1:617567/1‑41 (MQ=255)                                                              cGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCTTGCATGTAGTGga        <  1:180471/70‑1 (MQ=255)                                                              cGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGga        <  1:3045120/70‑1 (MQ=255)                                                              cGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGga        <  1:115108/70‑1 (MQ=255)                                                               ggATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGgatg      <  1:1051507/71‑1 (MQ=255)                                                               ggATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGgat       >  1:2498510/1‑70 (MQ=255)                                                               ggATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGgat       >  1:1712621/1‑70 (MQ=255)                                                                gATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGgatga     <  1:1510895/71‑1 (MQ=255)                                                                  tA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGatgatg   <  1:2111386/71‑1 (MQ=255)                                                                   a‑aCGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGt  >  1:1109247/1‑71 (MQ=255)                                                                   a‑aCGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGt  >  1:2848019/1‑71 (MQ=255)                                                                    |                                                                       TTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGT  >  minE/608631‑608767

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑