| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 975,257:1 | +A | 43.5% | coding (358/375 nt) | ydeH ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 975,257 | 1 | . | A | 43.5% | 6.9 / 156.9 | 69 | coding (358/375 nt) | ydeH | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base . (15/24); new base A (18/12); total (33/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.27e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
GAACCCGACGACCCGGCAATCCCGTCAAAACATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGT‑ATCGGTTAATGCCGCAGTAAAAGAAAGCAACCCCTCCTGAAAGGCGTCGAAATGCGCGTCCTGCCAGTGA > minE/975189‑975327 | gAACCCGACGACCCGGCAATCCCGTCAAAACATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGT‑At > 1:3878904/1‑71 (MQ=255)gAACCCGACGACCCGGCAATCCCGTCAAAACATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGT‑At > 1:3601563/1‑71 (MQ=255) ccGACGACCCGGCAATCCCGTCAAAACATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGT‑ATCGGt > 1:2437555/1‑71 (MQ=255) acgacCCGGCAATCCCGTCAAAACATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGT‑ATCGGTTaa < 1:3443201/71‑1 (MQ=255) acgacCCGGCAATCCCGTCAAAACATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGT‑ATCGGTTaa < 1:1912099/71‑1 (MQ=255) cgacCCGGCAATCCCGTCAAAACATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGT‑ATCGGTTAAt > 1:2389228/1‑71 (MQ=255) cgacCCGGCAATCCCGTCAAAACATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGT‑ATCGGTTAAt > 1:130917/1‑71 (MQ=255) acCCGGCAATCCCGTCAAAACATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGT‑ATCGGTTAATGc < 1:1407503/71‑1 (MQ=255) cccGGCAATCCCGTCAAAACATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGT‑ATCGGTTAATGcc > 1:3646093/1‑71 (MQ=255) cGGCAATCCCGTCAAAACATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGTAATCGGTTAATGCCg > 1:1404873/1‑71 (MQ=255) cGGCAATCCCGTCAAAACATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGT‑ATCGGTTAATGCCg > 1:788586/1‑70 (MQ=255) ggCAATCCCGTCAAAACATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGTAATCGGTTAATGCCg > 1:881086/1‑70 (MQ=255) ggCAATCCCGTCAAAACATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGTAATCGGTTAATGCCg > 1:3038044/1‑70 (MQ=255) ggCAATCCCGTCAAAACATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGT‑ATCGGTTAATGCCg > 1:1452960/1‑69 (MQ=255) gCAATCCCGTCAAAACATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGTAATCGGTTAATGCCg > 1:2763301/1‑69 (MQ=255) gCAATCCCGTCAAAACATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGTAATCGGTTAATGCCGCa < 1:3639760/71‑1 (MQ=255) gCAATCCCGTCAAAACATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGTAATCGGTTAATGCCGCa < 1:615850/71‑1 (MQ=255) aaTCCCGTCAAAACATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGT‑ATCGGTTAATGCCGCAGTa < 1:1376465/71‑1 (MQ=255) aTCCCGTCAAAACATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGT‑ATCGGTTAATGCCGCAGTaa < 1:2343895/71‑1 (MQ=255) tCCCGTCAAAACATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGTAATCGGTTAATg > 1:3686787/1‑62 (MQ=255) cGTCAAAACATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGTAATCGGTTAATGCCGCAGTaa > 1:2845407/1‑68 (MQ=255) cGTCAAAACATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGT‑ATCGGTTAATGCCGCAGTAaaaga < 1:3244414/71‑1 (MQ=255) gTCAAAACATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGT‑ATCGGTTAATGCCGCAGTAaaaga < 1:1856739/70‑1 (MQ=255) aaaaCATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGT‑ATCGGTTAATGCCGCAGTAAAAGAAAGc < 1:3813719/71‑1 (MQ=255) aaaaCATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGT‑ATCGGTTAATGCCGCAGTAAAAGAAAGc < 1:2792644/71‑1 (MQ=255) aaaCATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGTAATCGGTTAATGCCGCAGt > 1:3401939/1‑61 (MQ=255) aaaCATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGTAATCGGTTAATGCCGCAGTAAAAGAAAGc < 1:3847473/71‑1 (MQ=255) aaaCATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGT‑ATCGGTTAATGCCGCAGTAAAAGAAAGCa < 1:1826960/71‑1 (MQ=255) aTCCATATTGCTACGGATCGTCAGCAAATAAATTTTGT‑ATCGGTTaa > 1:2821104/1‑47 (MQ=255) ccATATTGCTACGGATCGTCAGCAAATAAATTTTGTAATCGGTTAATGCCGCAGTAaaagaaa > 1:1275683/1‑63 (MQ=255) ccATATTGCTACGGATCGTCAGCAAATAAATTTTGTAATCGGTTAATGCCGCAGTAAAAGAAAGCAAcccc > 1:1111070/1‑71 (MQ=255) ccATATTGCTACGGATCGTCAGCAAATAAATTTTGTAATCGGTTAATGCCGCAGTAAAAGAAAGCAAcccc > 1:2257694/1‑71 (MQ=255) cATATTGCTACGGATCGTCAGCAAATAAATTTTGTAATCGGTTAATGCCGCAGTAAAAGAAAGCAAcccc < 1:2933535/70‑1 (MQ=255) tatTGCTACGGATCGTCAGCAAATAAATTTTGTAATCGGTTAATGCCGCAGTAAAAGAAAGc < 1:3847284/62‑1 (MQ=255) ttGCTACGGATCGTCAGCAAATAAATTTTGT‑ATCGGTTaa > 1:3069977/1‑40 (MQ=255) ttGCTACGGATCGTCAGCAAATAAATTTTGT‑ATCGGTTAATGCCGCAGTAaaaga < 1:1196094/55‑1 (MQ=255) tGCTACGGATCGTCAGCAAATAAATTTTGTAATCGGt > 1:1959725/1‑37 (MQ=37) tGCTACGGATCGTCAGCAAATAAATTTTGTAATCGGTTaa > 1:2195304/1‑40 (MQ=38) gCTACGGATCGTCAGCAAATAAATTTTGTAATCGGTTAATGCCGCAGTAAAAGAAAGCAACCCCTCCTGa < 1:2723537/70‑1 (MQ=255) gCTACGGATCGTCAGCAAATAAATTTTGT‑ATCGGTTAATGCCGCAGTAAAAGAAAGCAACCCCTCCTGa > 1:3020899/1‑69 (MQ=255) cTACGGATCGTCAGCAAATAAATTTTGTAATCGGTTAATGCCGCAGTaaa < 1:3597050/50‑1 (MQ=255) cTACGGATCGTCAGCAAATAAATTTTGT‑ATCGGTTAATGCCg < 1:3208804/42‑1 (MQ=255) cTACGGATCGTCAGCAAATAAATTTTGT‑ATCGGTTAATGCCg < 1:676898/42‑1 (MQ=255) cTACGGATCGTCAGCAAATAAATTTTGT‑ATCGGTTAATGCCg < 1:1999922/42‑1 (MQ=255) cTACGGATCGTCAGCAAATAAATTTTGT‑ATCGGTTAATGCCGCAGTAaaagaaag > 1:591406/1‑55 (MQ=255) tACGGATCGTCAGCAAATAAATTTTGT‑ATCGTTTAATGCCGCAGTAAAAGAAAGCAAc < 1:1502967/58‑1 (MQ=255) tACGGATCGTCAGCAAATAAATTTTGT‑ATCGGTTAATGCCGCAGTAAAAGAAAGCAAc < 1:321851/58‑1 (MQ=255) tACGGATCGTCAGCAAATAAATTTTGT‑ATCGGTTAATGCCGCAGTAAAAGAAAGCAACCcctc > 1:1068004/1‑63 (MQ=255) cGGATCGTCAGCAAATAAATTTTGTAATCGGTTAATGCCGCAGTAAAAGAAAGCAACCCCTCCTGAAAg < 1:3683191/69‑1 (MQ=255) cGGATCGTCAGCAAATAAATTTTGTAATCGGTTAATGCCGCAGTAAAAGAAAGCAACCCCTCCTGAAAGGc > 1:3944412/1‑71 (MQ=255) gTCAGCAAATAAATTTTGTAATCGGTTAATGCCGCAGTAAAAGAAAGCAACCCCTCCTGAAAGGCGTCGaa < 1:669875/71‑1 (MQ=255) gTCAGCAAATAAATTTTGTAATCGGTTAATGCCGCAGTAAAAGAAAGCAACCCCTCCTGAAAGGCGTCGaa < 1:1653402/71‑1 (MQ=255) tCAGCAAATAAATTTTGT‑ATCGGTTAATGCCGCAGTAAAAGAAAGCAACCCCTCCTGAAAGGCGTCGAAAt < 1:2336573/71‑1 (MQ=255) tCAGCAAATAAATTTTGT‑ATCGGTTAATGCCGCAGTAAAAGAAAGCAACCCCTCCTGAAAGGCGTCGAAAt < 1:620087/71‑1 (MQ=255) aGCAAATAAATTTTGTAATCGGTTAATGCCGCAGTAAAAGAAAGCAACCCCTCCTGAAAGGCGTCGAAATg > 1:2094386/1‑71 (MQ=255) aGCAAATAAATTTTGT‑ATCGGTTAATGCCGCAGTAAAAGAAAGCAACCCCTCCTGAAAGGCGTCGAAATgc < 1:1526653/71‑1 (MQ=255) aGCAAATAAATTTTGT‑ATCGGTTAATGCCGCAGTAAAAGAAAGCAACCCCTCCTGAAAGGCGTCGAAATgc < 1:892505/71‑1 (MQ=255) aGCAAATAAATTTTGT‑ATCGGTTAATGCCGCAGTAAAAGAAAGCAACCCCTCCTGAAAGGCGTCGAAATg < 1:1644395/70‑1 (MQ=255) gCAAATAAATTTTGTAATCGGTTAATGCCGCAGTAAAAGAAAGCAACCCCTCCTGAAAGGCGt < 1:649113/63‑1 (MQ=255) taaatTTTGTAATCGGTTAATGCCGCAGTAAAAGAAAGCAACCCCTCCTGAAAGGCGTCGAAATg < 1:948106/65‑1 (MQ=255) aaatTTTGTAATCGGTTAATGCCGCAGTAAAAGAAAGCAACCCCTCCTGAAAGGCGTCGAAATgc > 1:3353168/1‑65 (MQ=255) aaatTTTGTAATCGGTTAATGCCGCAGTAAAAGAAAGCAACCCCTCCTGAAAGGCGTCGAAATGCGCGTcc > 1:2800911/1‑71 (MQ=255) aatTTTGT‑ATCGGTTAATGCCGCAGTAAAAGAAAGCaa > 1:1762927/1‑38 (MQ=255) aatTTTGT‑ATCGGTTAATGCCGCAGTAAAAGAAAGCaa > 1:801386/1‑38 (MQ=255) atTTTGTAATCGGTTAATGCCGCAGTAAAAGAAAGc > 1:1800650/1‑36 (MQ=37) atTTTGTAATCGGTTAATGCCGCAGTAAAAGAAAGCAAccc > 1:1350853/1‑41 (MQ=38) ttttGT‑ATCGGTTAATGCCGCAGTAAAAGAAAGCAACCCCTCCTGAAAGGCGTCGAAAt < 1:1236784/59‑1 (MQ=255) ttttGT‑ATCGGTTAATGCCGCAGTAAAAGAAAGCAACCCCTCCTGAAAGGCGTCGAAAt < 1:2770408/59‑1 (MQ=255) ttttGT‑ATCGGTTAATGCCGCAGTAAAAGAAAGCAACCCCTCCTGAAAGGCGTCGAAATGCGCGTCCTGcc < 1:719016/71‑1 (MQ=255) t‑ATCGGTTAATGCCGCAGTAAAAGAAAGCAACCCCTCCTGAAAGGCGTCGAAATGCGCGTCCTGCCAGTGa < 1:3532633/71‑1 (MQ=255) | GAACCCGACGACCCGGCAATCCCGTCAAAACATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGT‑ATCGGTTAATGCCGCAGTAAAAGAAAGCAACCCCTCCTGAAAGGCGTCGAAATGCGCGTCCTGCCAGTGA > minE/975189‑975327 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |