Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	63,052	A→T	7.3%	V136D (GTT→GAT)	polB ←	DNA polymerase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	63,052	0	A	T	7.3%	150.2 / 4.0	55	V136D (GTT→GAT)	polB	DNA polymerase II
Reads supporting (aligned to +/- strand):  ref base A (34/17);  new base T (2/2);  total (36/19)
Fisher's exact test for biased strand distribution p-value = 6.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01

TCAATATCTATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAG  >  minE/62986‑63120                                                                   |                                                                     tCAATATCTATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGa                                                                   >  1:926905/1‑70 (MQ=255) tCAATATCTATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGa                                                                   >  1:520038/1‑70 (MQ=255) tCAATATCTATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGa                                                                   >  1:3108879/1‑70 (MQ=255)  cAATATCTATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGa                                                                   >  1:101153/1‑69 (MQ=255)  cAATATCTATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGa                                                                   >  1:3644107/1‑69 (MQ=255)  cAATATCTATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGa                                                                   >  1:1605203/1‑69 (MQ=255)           taGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGTGCCa                                                           <  1:640950/68‑1 (MQ=255)           taGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGTGCCATtg                                                        <  1:1257820/71‑1 (MQ=255)           taGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGTGCCATtg                                                        <  1:3915707/71‑1 (MQ=255)             gAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGTGCCATtgt                                                       <  1:34236/70‑1 (MQ=255)               aaCCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGTGCCATTGTGCa                                                    <  1:1748270/71‑1 (MQ=255)                aCCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGTGCCATTGTGCat                                                   <  1:3799938/71‑1 (MQ=255)                aCCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGTGCCATTGTGCa                                                    >  1:1490486/1‑70 (MQ=255)                aCCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGTGCCATTGTGCa                                                    >  1:2907924/1‑70 (MQ=255)                  ccACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGTGCCATTGTGCatat                                                 <  1:1234864/71‑1 (MQ=255)                    aCTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGTGCCATTGTGCATATCa                                               >  1:2055238/1‑71 (MQ=255)                       tGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGTGCCATTGTGCATATCAcc                                             >  1:2833113/1‑70 (MQ=255)                         aGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGa                                                                   <  1:2882542/46‑1 (MQ=255)                         aGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGa                                                                   <  1:3897010/46‑1 (MQ=255)                         aGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGa                                                                   <  1:2809462/46‑1 (MQ=255)                             gcggACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGTGCCATTGTGCATATCACCCTCGAcc                                      >  1:3209140/1‑71 (MQ=255)                                   gATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGTGCCATTGTGCATATCACCCTCGACCCACAcc                                <  1:384981/71‑1 (MQ=255)                                      aGTCGGGATGCGGTTTCAGACGGGCATTAACGATAg                                                                <  1:3559714/36‑1 (MQ=255)                                      aGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGTGCCATTGTGCATATCAcc                                             >  1:2832398/1‑55 (MQ=255)                                      aGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCggt                             >  1:2264294/1‑71 (MQ=255)                                       gTCGGGATGCGGTTTCAGACGGGCATTAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCggt                             >  1:3730974/1‑70 (MQ=255)                                        tCGGGATGCGGTTTCAGACGGGCATTAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCggtga                           <  1:945813/71‑1 (MQ=255)                                         cGGGATGCGGTTTCAGACGGGCATTAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCggtgag                          >  1:3543699/1‑71 (MQ=255)                                          gggATGCGGTTTCAGACGGGCATTAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCggtgagg                         >  1:2835111/1‑71 (MQ=255)                                          gggATGCGGTTTCAGACGGGCATTAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCggtgagg                         >  1:1573966/1‑71 (MQ=255)                                                cGGTTTCAGACGGGCATTAACGATAGTGCCATTGTGCata                                                  >  1:892390/1‑40 (MQ=255)                                                cGGTTTCAGACGGGCATTAACGATAGTGCCATTGTGCata                                                  >  1:1244860/1‑40 (MQ=255)                                                cGGTTTCAGACGGGCATTAACGATAGTGCCATTGTGCata                                                  >  1:3665811/1‑40 (MQ=255)                                                cGGTTTCAGACGGGCATTAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATa                    >  1:3241377/1‑70 (MQ=255)                                                 ggTTTCAGACGGGCATTAACGATAGTGCCATTGTGCATATCACCCTCGACCca                                    >  1:386437/1‑53 (MQ=255)                                                 ggTTTCAGACGGGCATTAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATaaa                  >  1:2351909/1‑71 (MQ=255)                                                     tCAGACGGGGATTAACGATAGTGCCATTGTGCATATCACCCTc                                          >  1:2619606/1‑43 (MQ=255)                                                     tCAGACGGGCATTAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGc              >  1:2082603/1‑71 (MQ=255)                                                      cAGACGGGCATTAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCt             >  1:328870/1‑71 (MQ=255)                                                       aGACGGGCATTAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTc            >  1:2606051/1‑71 (MQ=255)                                                         aCGGGCATTAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCa          <  1:215644/71‑1 (MQ=255)                                                          cGGGCATTAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCAt         <  1:729795/71‑1 (MQ=255)                                                          atggCACTATCGTTAATGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCAt         <  1:3883913/69‑1 (MQ=38)                                                          atggCACTATCGTTAATGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCAt         <  1:697070/69‑1 (MQ=38)                                                           tggCACTATCGTTAATGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCAt         >  1:1814764/2‑70 (MQ=255)                                                           tggCACTATCGTTAATGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCAt         >  1:1882755/2‑70 (MQ=255)                                                              cATTAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCAt         >  1:708358/1‑67 (MQ=255)                                                              cATTAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGa     <  1:1354054/71‑1 (MQ=255)                                                              cATTAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGa     <  1:2852539/71‑1 (MQ=255)                                                               aTTAACGATAGTGCCATTGTGCATATCACCCTCGCCCCACACCGGTGAGGTGATAAACCGCTCCATCAGa     >  1:1959647/1‑70 (MQ=255)                                                               aTTAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCAt         >  1:2826842/1‑66 (MQ=255)                                                                ttAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGa     >  1:1030964/1‑69 (MQ=255)                                                                ttAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGa     >  1:685845/1‑69 (MQ=255)                                                                 tAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAAc                 >  1:3456679/1‑56 (MQ=255)                                                                 tAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAg  >  1:1234885/1‑71 (MQ=255)                                                                  aaCGATAGTGCCATTGTGCATATCACCCTCGACCcaca                                  >  1:1384704/1‑38 (MQ=255)                                                                  aaCGATAGTGCCATTGTGCATATCACCCTCGACCCACACCggtgag                          <  1:1403659/46‑1 (MQ=255)                                                                  aaCGATAGTGCCATTGTGCATATCACCCTCGACCCACACCggtgag                          >  1:3372039/1‑46 (MQ=255)                                                                  aaCGATAGTGCCATTGTGCATATCACCCTCGACCCACACCg                               >  1:412935/1‑41 (MQ=255)                                                                   aCGATAGTGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAAcc                <  1:1003400/55‑1 (MQ=255)                                                                   aCGATAGTGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAAcc                <  1:3712400/55‑1 (MQ=255)                                                                   |                                                                     TCAATATCTATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAG  >  minE/62986‑63120

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑