| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,015,039 | T→G | 12.5% | K344Q (AAA→CAA) ‡ | fumA ← | aerobic Class I fumarate hydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,015,039 | 0 | T | G | 12.5% | 117.4 / 15.2 | 56 | K344Q (AAA→CAA) ‡ | fumA | aerobic Class I fumarate hydratase |
| Reads supporting (aligned to +/- strand): ref base T (25/24); new base G (2/5); total (27/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.24e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.32e-01 | |||||||||||
CGGACGGTTAAGGTCAACGCGCACCGCTTCGCCTTCTCCCGCTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTC > minE/1014971‑1015108 | cGGACGGTTAAGGTCAACGCGCACCGCTTCGCCTTCTCCCGCTTTGCGCAGCTCTTCCGGGATATATTTGc > 1:1917220/1‑71 (MQ=255) ggACGGTTAAGGTCAACGCGCACCGCTTCGCCTTCTCCCGCTTTGCGCAGCTCTTCCGGGATATATTTGcc > 1:1986686/1‑71 (MQ=255) ggACGGTTAAGGTCAACGCGCACCGCTTCGCCTTCTCCCGCTTTGCGCAGCTCTTCCGGGATATATTTGc < 1:150915/70‑1 (MQ=255) aCGGTTAAGGTCAACGCGCACCGCTTCGCCTTCTCCCGCTTTGCGCAGCTCTTCCGGGATATATTTGCCTg < 1:1512345/71‑1 (MQ=255) gTTAAGGTCAACTTTCACCGCTTCGCCTTCACCGGCCTGGCGCAGTTCTTGTGGAATGTACTGGCCTgggt < 1:1731115/71‑3 (MQ=255) tAAGGTCAACGCGCACCGCTTCGCCTTCTCCCGCTTTGCGCAGCTCTTCCGGGATATATTTGCCTg > 1:1941656/1‑66 (MQ=255) aaGGTCAACGCGCACCGCTTCGCCTTCTCCCGCTTTGCGCAGCTCTTCCGGGATATAttt < 1:3523031/60‑1 (MQ=255) aaGGTCAACGCGCACCGCTTCGCCTTCTCCCGCTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTAt > 1:1254363/1‑71 (MQ=255) aGGTCAACGCGCACCGCTTCGCCTTCTCCCGCTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATg > 1:16646/1‑71 (MQ=255) cAACGCGCACCGCTTCGCCTTCTCCCGCTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGtt > 1:2462200/1‑69 (MQ=255) aaCGCGCACCGCTTCGCCTTCTCCCGCTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTc > 1:2295325/1‑69 (MQ=255) tttcACCGCTTCGCCTTCACCGGCCTGGCGCAGTTCTTGTGGAATGTACTGGCCTGGGTTGTGTTCCAGtt > 1:3721710/4‑71 (MQ=255) ttcACCGCTTCGCCTTCACCGGCCTGGCGCAGTTCTTGTGGAATGTACTGGCCTGGGTTGTGTTCCAGttt > 1:787777/3‑71 (MQ=255) cgcACCGCTTCGGCTTCTGCCGCTTTTCGCAGCTCTTCCGGGATATATTTGCCTGGATTTTGTTCCAGt < 1:2163730/69‑1 (MQ=255) tcACCGCTTCGCCTTCACCGGCCTGGCGCAGTTCTTGTGGAATGTACTGGCCTGGGTTGTGTTCCAGttt < 1:47357/69‑1 (MQ=255) cACCGCTTCGCCTTCTCCCGCTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGttttt > 1:2314172/1‑71 (MQ=255) aCCGCTTCGCCTTCTCCCGCTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTc > 1:3517774/1‑71 (MQ=255) ccGCTTCGCCTTCACCGGCCTGGCGCAGTTCTTGTGGAATGTACTGGCCTGGGTTGTGTTCCAGTTTTTCg < 1:2290998/71‑1 (MQ=255) cGCTTCGCCTTCTCCCGCTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGa < 1:692116/71‑1 (MQ=255) cTTCGCCTTCTCCCGCTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATc < 1:437175/71‑1 (MQ=255) cTTCGCCTTCACCGGCCTGGCGCAGTTCTTGTGGAATGTACTGGCCTGGGTTGTGTTCCAGttt < 1:1406777/64‑1 (MQ=255) cGCCTTCTCCCGCTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCa < 1:1165647/70‑1 (MQ=255) cGCCTTCTCCCGCTTTGCCCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCa < 1:2988440/70‑1 (MQ=255) ccTTCTCCCGCTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGt < 1:108948/57‑1 (MQ=255) cTTCTCCCGCTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATc > 1:1594628/1‑71 (MQ=255) cTTCTCCCGCTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATc > 1:1125790/1‑71 (MQ=255) ttCTCCCGCTTTGCGCAGCTCTTCCGGGATATAttt < 1:1315451/36‑1 (MQ=255) ttCTCCCGCTTTGCGCAGCTCTTCCGGGATATATTTGCCTTGATTAt < 1:2380/47‑1 (MQ=255) tctcCCGCTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGt > 1:1497999/1‑48 (MQ=255) ctcCCGCTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATc > 1:3728427/1‑68 (MQ=255) tcCCGCTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGAt > 1:1121387/1‑66 (MQ=255) tcCCGCTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGAt > 1:1125932/1‑66 (MQ=255) ccGCTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGtt < 1:2576907/51‑1 (MQ=255) ccGCTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTg < 1:630993/70‑1 (MQ=255) ccGCTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGa < 1:1215642/71‑1 (MQ=255) gCTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACg > 1:2947824/1‑71 (MQ=255) gCTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACg > 1:1491615/1‑71 (MQ=255) gCTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACg > 1:2299975/1‑71 (MQ=255) cTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGa < 1:3915817/54‑1 (MQ=255) ttGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGtt > 1:1209672/1‑71 (MQ=255) ttGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGtt > 1:3504198/1‑71 (MQ=255) tGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGttttt < 1:381257/48‑1 (MQ=255) cgcAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCt < 1:3868742/61‑1 (MQ=255) gcAGTTCTTGTGGAATGTACTGGCCTGGGTTGTGTTCCAGTTTTTCGATCCAGATACCTTCGCGGGTGAtt < 1:3451835/71‑2 (MQ=255) gcAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGa < 1:1638161/48‑1 (MQ=255) gcAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGa < 1:292661/69‑1 (MQ=255) aGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCtt > 1:1936973/1‑71 (MQ=255) gCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTc > 1:2642122/1‑71 (MQ=255) ttCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCg < 1:2554865/40‑1 (MQ=255) ttCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCg < 1:2120987/40‑1 (MQ=255) ttCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGc < 1:1835960/69‑1 (MQ=255) ccGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCttt > 1:1426032/1‑70 (MQ=255) ccGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCttt > 1:2298172/1‑70 (MQ=255) ccGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCt < 1:2611960/68‑1 (MQ=255) tatatTTGCCTGGATTATGTTCCAGTTTTTCGATCCa < 1:3802083/37‑1 (MQ=255) tatatTTGCCTGGATTATGTTCCAGTTTTTCGATCCa < 1:2543341/37‑1 (MQ=255) tatatTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGt < 1:3249398/51‑1 (MQ=255) atatTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTAc > 1:614520/1‑71 (MQ=255) tttGCCTGGATTATGTTCCAGTTTTTCGATCCAGAt > 1:1497605/1‑36 (MQ=255) tttGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTg < 1:498842/49‑1 (MQ=255) tGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTc < 1:3580333/70‑1 (MQ=255) | CGGACGGTTAAGGTCAACGCGCACCGCTTCGCCTTCTCCCGCTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTC > minE/1014971‑1015108 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |