| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,015,088 | C→T | 22.2% | K327K (AAG→AAA) | fumA ← | aerobic Class I fumarate hydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,015,088 | 0 | C | T | 22.2% | 143.4 / 35.1 | 72 | K327K (AAG→AAA) | fumA | aerobic Class I fumarate hydratase |
| Reads supporting (aligned to +/- strand): ref base C (32/24); new base T (8/8); total (40/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.76e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01 | |||||||||||
AGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGT > minE/1015020‑1015152 | aGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCtt > 1:1936973/1‑71 (MQ=255) gCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTc > 1:2642122/1‑71 (MQ=255) ttCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGc < 1:1835960/69‑1 (MQ=255) ccGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCttt > 1:2298172/1‑70 (MQ=255) ccGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCttt > 1:1426032/1‑70 (MQ=255) ccGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCt < 1:2611960/68‑1 (MQ=255) atatTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTAc > 1:614520/1‑71 (MQ=255) tGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTc < 1:3580333/70‑1 (MQ=255) gCCTGGGTTGTGTTCCAGTTTTTCGATCCAGATACCTTCGCGGTTGATTTTCGCttt < 1:3234085/57‑1 (MQ=255) ccTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTcag < 1:1789955/70‑1 (MQ=255) tGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGa < 1:2661297/56‑1 (MQ=255) tGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGa < 1:2291907/56‑1 (MQ=255) tGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTcag > 1:2800083/1‑68 (MQ=255) tGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTcag > 1:834441/1‑68 (MQ=255) tGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTc < 1:2398348/66‑1 (MQ=255) tGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTc < 1:155690/66‑1 (MQ=255) ggATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTcagca > 1:2212769/1‑69 (MQ=255) gATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGata > 1:266991/1‑56 (MQ=255) gATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTcagcag < 1:1247317/69‑1 (MQ=255) gATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCagcagag < 1:1056671/71‑1 (MQ=255) aTTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTcagcag < 1:1669408/68‑1 (MQ=255) tATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTa > 1:1125117/1‑56 (MQ=255) tATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCagcagagcag > 1:2973981/1‑71 (MQ=255) tATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCagcagagc < 1:1669752/69‑1 (MQ=255) gtGTTCCAGTTTTTCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTCagcggagcag < 1:721844/69‑1 (MQ=255) tGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAgg < 1:3892848/70‑1 (MQ=255) gTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCagcagag > 1:392659/1‑65 (MQ=255) gTTCCAGTTTTTCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTca > 1:1812360/1‑59 (MQ=255) ttCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTcagca < 1:2643762/61‑1 (MQ=255) ttCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGgaga < 1:3764014/71‑1 (MQ=255) ttCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGgaga > 1:607970/1‑71 (MQ=255) tCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCt < 1:2805696/42‑1 (MQ=255) cAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGc > 1:78816/1‑71 (MQ=255) cAGTTTTTCGATCCAGATACCTTCGCGGTTGATTTTCGCttt < 1:243883/42‑1 (MQ=19) aGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTAGATATTACGGTCagcagag < 1:2524783/60‑1 (MQ=255) tttttCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGt < 1:1312897/50‑1 (MQ=255) tttttCGATCCAGATACCTTCGCGGTTGATTTTCg < 1:115257/35‑1 (MQ=12) tttttCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGGAGACGccc < 1:793432/70‑1 (MQ=255) ttttCGATCCAGATCCCCTGACGGTTGATCTTCGc > 1:1709663/1‑35 (MQ=255) tttCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTcagca > 1:2012158/1‑53 (MQ=255) tttCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGga > 1:2102585/1‑61 (MQ=255) tttCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGc > 1:2842329/1‑66 (MQ=255) tttCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATa > 1:2452796/1‑71 (MQ=255) tttCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCAtg > 1:3208825/1‑70 (MQ=255) tttCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCAtg > 1:3156853/1‑70 (MQ=255) tCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGata > 1:961996/1‑39 (MQ=255) tCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGga < 1:2499349/59‑1 (MQ=255) tCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAgg > 1:3667360/1‑58 (MQ=255) tCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAgg > 1:2734118/1‑58 (MQ=255) ccAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATaccgaccg > 1:3135370/1‑71 (MQ=255) cAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCgg > 1:1743945/1‑71 (MQ=255) gATCCCCTGACTGTTTATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCggg < 1:3775032/70‑1 (MQ=255) aTCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGccc > 1:2886572/1‑57 (MQ=255) aTCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGccc > 1:2632534/1‑57 (MQ=255) tCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCa > 1:2387389/1‑57 (MQ=255) cccTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAgg < 1:241152/70‑1 (MQ=255) cccTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGa > 1:3128745/1‑71 (MQ=255) cccTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGa > 1:2455545/1‑71 (MQ=255) ccTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGAt < 1:2456632/71‑1 (MQ=255) gACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGgaga > 1:215618/1‑45 (MQ=255) gACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGga < 1:2911233/43‑1 (MQ=255) gACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATaccgac > 1:1989042/1‑58 (MQ=255) cgcGGTTGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGATgc > 1:1496659/3‑70 (MQ=255) gcGGTTGATTTTAGCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCAt > 1:1824979/2‑51 (MQ=255) aCGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATgcgc < 1:3068290/71‑1 (MQ=255) ttGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGa < 1:1931279/62‑1 (MQ=255) ttGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGATGCGcc < 1:1287592/68‑1 (MQ=255) ttGATCTTCGCTTTGATATTACGGTCAGCAGATCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGt > 1:353839/1‑70 (MQ=255) tGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGa < 1:1551877/61‑1 (MQ=255) tGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCa > 1:821960/1‑45 (MQ=255) gATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCATgccgaccg > 1:1015337/1‑53 (MQ=255) gATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATa > 1:3037636/1‑46 (MQ=255) | AGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGT > minE/1015020‑1015152 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |