| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,015,112 | A→G | 28.3% | S319S (TCT→TCC) | fumA ← | aerobic Class I fumarate hydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,015,112 | 0 | A | G | 28.3% | 87.3 / 40.8 | 60 | S319S (TCT→TCC) | fumA | aerobic Class I fumarate hydratase |
| Reads supporting (aligned to +/- strand): ref base A (28/15); new base G (7/10); total (35/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.45e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGT > minE/1015044‑1015182 | ggATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTcagca > 1:2212769/1‑69 (MQ=255) gATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTcagcag < 1:1247317/69‑1 (MQ=255) gATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCagcagag < 1:1056671/71‑1 (MQ=255) aTTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTcagcag < 1:1669408/68‑1 (MQ=255) tATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCagcagagcag > 1:2973981/1‑71 (MQ=255) tATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCagcagagc < 1:1669752/69‑1 (MQ=255) gtGTTCCAGTTTTTCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTCagcggagcag < 1:721844/69‑1 (MQ=255) tGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAgg < 1:3892848/70‑1 (MQ=255) gTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCagcagag > 1:392659/1‑65 (MQ=255) ttCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTcagca < 1:2643762/61‑1 (MQ=255) ttCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGgaga > 1:607970/1‑71 (MQ=255) ttCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGgaga < 1:3764014/71‑1 (MQ=255) cAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGc > 1:78816/1‑71 (MQ=255) aGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTAGATATTACGGTCagcagag < 1:2524783/60‑1 (MQ=255) tttttCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGGAGACGccc < 1:793432/70‑1 (MQ=255) tttCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTcagca > 1:2012158/1‑53 (MQ=255) tttCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGga > 1:2102585/1‑61 (MQ=255) tttCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGc > 1:2842329/1‑66 (MQ=255) tttCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATa > 1:2452796/1‑71 (MQ=255) tttCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCAtg > 1:3208825/1‑70 (MQ=255) tttCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCAtg > 1:3156853/1‑70 (MQ=255) tCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGga < 1:2499349/59‑1 (MQ=255) tCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAgg > 1:2734118/1‑58 (MQ=255) tCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAgg > 1:3667360/1‑58 (MQ=255) ccAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATaccgaccg > 1:3135370/1‑71 (MQ=255) cAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCgg > 1:1743945/1‑71 (MQ=255) gATCCCCTGACTGTTTATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCggg < 1:3775032/70‑1 (MQ=255) aTCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGccc > 1:2632534/1‑57 (MQ=255) aTCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGccc > 1:2886572/1‑57 (MQ=255) tCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCa > 1:2387389/1‑57 (MQ=255) cccTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAgg < 1:241152/70‑1 (MQ=255) cccTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGa > 1:2455545/1‑71 (MQ=255) cccTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGa > 1:3128745/1‑71 (MQ=255) ccTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGAt < 1:2456632/71‑1 (MQ=255) gACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGgaga > 1:215618/1‑45 (MQ=255) gACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGga < 1:2911233/43‑1 (MQ=255) gACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATaccgac > 1:1989042/1‑58 (MQ=255) cgcGGTTGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGATgc > 1:1496659/3‑70 (MQ=255) gcGGTTGATTTTAGCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCAt > 1:1824979/2‑51 (MQ=255) aCGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATgcgc < 1:3068290/71‑1 (MQ=255) ttGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGa < 1:1931279/62‑1 (MQ=255) ttGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGATGCGcc < 1:1287592/68‑1 (MQ=255) ttGATCTTCGCTTTGATATTACGGTCAGCAGATCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGt > 1:353839/1‑70 (MQ=255) tGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGa < 1:1551877/61‑1 (MQ=255) tGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCa > 1:821960/1‑45 (MQ=255) gATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCATgccgaccg > 1:1015337/1‑53 (MQ=255) gATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATa > 1:3037636/1‑46 (MQ=255) ttCGCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTg < 1:3726174/70‑1 (MQ=255) tCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTgg > 1:996414/1‑70 (MQ=255) tCGCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTgg < 1:3781144/70‑1 (MQ=255) cGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTg < 1:287031/68‑1 (MQ=255) gCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCa > 1:587563/1‑37 (MQ=255) gCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCa > 1:2516929/1‑37 (MQ=255) gCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCa < 1:3384544/70‑1 (MQ=255) cTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATa > 1:3394296/1‑38 (MQ=255) tttGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACg < 1:1846925/63‑1 (MQ=255) ttGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCgg < 1:2159129/44‑1 (MQ=255) ttGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCgg > 1:759547/1‑44 (MQ=255) tGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGc < 1:903527/69‑1 (MQ=255) atatTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAAt < 1:3508987/71‑1 (MQ=255) atTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCa > 1:683627/1‑71 (MQ=255) atTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCa > 1:2950878/1‑71 (MQ=255) ttACGGTCAGCAGAGCAGGAGACGCCCATAACGACCGGGCAGGATGCGCCGt > 1:3046932/1‑52 (MQ=255) tACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCa > 1:3438422/1‑40 (MQ=255) tACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCa < 1:246968/60‑1 (MQ=255) tACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCAcg > 1:67741/1‑71 (MQ=255) tACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCAc > 1:1604791/1‑70 (MQ=255) cGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCAcgc < 1:2385778/70‑1 (MQ=255) ggTCAGCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGAtaa < 1:2664253/66‑3 (MQ=255) gTCAGCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAAcgcg < 1:3702762/69‑1 (MQ=255) tCAGCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAAt < 1:458145/71‑1 (MQ=255) agcggagcagGAGACGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTg < 1:1444721/50‑1 (MQ=255) agcagagcagGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGt > 1:1140145/1‑71 (MQ=255) gcagagcagGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTc > 1:613430/1‑71 (MQ=255) agagcagGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGt < 1:3294539/71‑1 (MQ=255) | GGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGT > minE/1015044‑1015182 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |