| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,015,130 | A→G | 30.6% | G313G (GGT→GGC) | fumA ← | aerobic Class I fumarate hydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,015,130 | 0 | A | G | 30.6% | 77.2 / 47.2 | 62 | G313G (GGT→GGC) | fumA | aerobic Class I fumarate hydratase |
| Reads supporting (aligned to +/- strand): ref base A (19/24); new base G (5/14); total (24/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.60e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCAC > minE/1015060‑1015200 | tttCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATa > 1:2452796/1‑71 (MQ=255) ccAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATaccgaccg > 1:3135370/1‑71 (MQ=255) cAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCgg > 1:1743945/1‑71 (MQ=255) gATCCCCTGACTGTTTATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCggg < 1:3775032/70‑1 (MQ=255) cccTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAgg < 1:241152/70‑1 (MQ=255) cccTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGa > 1:2455545/1‑71 (MQ=255) cccTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGa > 1:3128745/1‑71 (MQ=255) ccTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGAt < 1:2456632/71‑1 (MQ=255) gACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATaccgac > 1:1989042/1‑58 (MQ=255) cgcGGTTGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGATgc > 1:1496659/3‑70 (MQ=255) aCGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATgcgc < 1:3068290/71‑1 (MQ=255) ttGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGa < 1:1931279/62‑1 (MQ=255) ttGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGATGCGcc < 1:1287592/68‑1 (MQ=255) ttGATCTTCGCTTTGATATTACGGTCAGCAGATCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGt > 1:353839/1‑70 (MQ=255) tGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGa < 1:1551877/61‑1 (MQ=255) gATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCATgccgaccg > 1:1015337/1‑53 (MQ=255) gATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATa > 1:3037636/1‑46 (MQ=255) ttCGCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTg < 1:3726174/70‑1 (MQ=255) tCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTgg > 1:996414/1‑70 (MQ=255) tCGCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTgg < 1:3781144/70‑1 (MQ=255) cGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTg < 1:287031/68‑1 (MQ=255) gCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCa < 1:3384544/70‑1 (MQ=255) cTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATa > 1:3394296/1‑38 (MQ=255) tttGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACg < 1:1846925/63‑1 (MQ=255) ttGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCgg < 1:2159129/44‑1 (MQ=255) ttGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCgg > 1:759547/1‑44 (MQ=255) tGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGc < 1:903527/69‑1 (MQ=255) atatTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAAt < 1:3508987/71‑1 (MQ=255) atTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCa > 1:683627/1‑71 (MQ=255) atTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCa > 1:2950878/1‑71 (MQ=255) ttACGGTCAGCAGAGCAGGAGACGCCCATAACGACCGGGCAGGATGCGCCGt > 1:3046932/1‑52 (MQ=255) tACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCa > 1:3438422/1‑40 (MQ=255) tACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCa < 1:246968/60‑1 (MQ=255) tACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCAcg > 1:67741/1‑71 (MQ=255) tACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCAc > 1:1604791/1‑70 (MQ=255) cGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCAcgc < 1:2385778/70‑1 (MQ=255) ggTCAGCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGAtaa < 1:2664253/66‑3 (MQ=255) gTCAGCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAAcgcg < 1:3702762/69‑1 (MQ=255) tCAGCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAAt < 1:458145/71‑1 (MQ=255) agcggagcagGAGACGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTg < 1:1444721/50‑1 (MQ=255) agcagagcagGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGt > 1:1140145/1‑71 (MQ=255) gcagagcagGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTc > 1:613430/1‑71 (MQ=255) agagcagGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGt < 1:3294539/71‑1 (MQ=255) gagcagGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGt > 1:3061506/1‑70 (MQ=255) gagcagGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTg < 1:2411039/71‑1 (MQ=255) agcagGAGACGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTg < 1:3470384/45‑1 (MQ=255) agcagGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTg < 1:1499837/70‑1 (MQ=255) cagGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAg < 1:2901779/47‑1 (MQ=255) cagGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAg < 1:949085/70‑1 (MQ=255) agGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCgg < 1:2169736/59‑1 (MQ=255) agGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCg < 1:1398604/71‑1 (MQ=255) ggAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGc < 1:3311337/69‑1 (MQ=255) gagaCGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGaa < 1:1268342/71‑1 (MQ=255) gagaCGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCg < 1:1711353/69‑1 (MQ=255) gagaCGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCg < 1:2058641/69‑1 (MQ=255) agaCGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAg < 1:677158/71‑1 (MQ=255) agaCGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAg < 1:3228572/71‑1 (MQ=255) gaCGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAg > 1:1438358/1‑70 (MQ=255) gaCGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAg > 1:964574/1‑70 (MQ=255) aCGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTa > 1:1438279/1‑71 (MQ=255) aCGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTa > 1:6168/1‑71 (MQ=255) aCGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAg < 1:2903650/41‑1 (MQ=255) cccATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTAtt < 1:3245503/70‑1 (MQ=255) ccATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTa > 1:717175/1‑71 (MQ=255) ccATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTa > 1:747980/1‑71 (MQ=255) cATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTac < 1:3696802/71‑1 (MQ=255) tACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAg > 1:2835182/1‑57 (MQ=255) tACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTacca > 1:1057730/1‑71 (MQ=255) accgaccgGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTaccac > 1:1268200/1‑71 (MQ=255) accgaccgGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTaccac > 1:3530167/1‑71 (MQ=255) accgaccgGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTacca > 1:2071780/1‑70 (MQ=255) | TTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCAC > minE/1015060‑1015200 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |