| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,015,175 | G→A | 27.7% | I298I (ATC→ATT) | fumA ← | aerobic Class I fumarate hydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,015,175 | 0 | G | A | 27.7% | 130.5 / 56.0 | 83 | I298I (ATC→ATT) | fumA | aerobic Class I fumarate hydratase |
| Reads supporting (aligned to +/- strand): ref base G (28/32); new base A (11/12); total (39/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01 | |||||||||||
TCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGAT > minE/1015107‑1015237 | tCAGCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAAt < 1:458145/71‑1 (MQ=255) agcagagcagGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGt > 1:1140145/1‑71 (MQ=255) gcagagcagGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTc > 1:613430/1‑71 (MQ=255) agagcagGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGt < 1:3294539/71‑1 (MQ=255) gagcagGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGt > 1:3061506/1‑70 (MQ=255) gagcagGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTg < 1:2411039/71‑1 (MQ=255) agcagGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTg < 1:1499837/70‑1 (MQ=255) cagGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAg < 1:949085/70‑1 (MQ=255) agGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCgg < 1:2169736/59‑1 (MQ=255) agGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCg < 1:1398604/71‑1 (MQ=255) ggAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGc < 1:3311337/69‑1 (MQ=255) gagaCGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGaa < 1:1268342/71‑1 (MQ=255) gagaCGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCg < 1:2058641/69‑1 (MQ=255) gagaCGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCg < 1:1711353/69‑1 (MQ=255) agaCGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAg < 1:3228572/71‑1 (MQ=255) agaCGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAg < 1:677158/71‑1 (MQ=255) gaCGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAg > 1:964574/1‑70 (MQ=255) gaCGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAg > 1:1438358/1‑70 (MQ=255) aCGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTa > 1:6168/1‑71 (MQ=255) aCGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTa > 1:1438279/1‑71 (MQ=255) cccATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTAtt < 1:3245503/70‑1 (MQ=255) ccATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTa > 1:747980/1‑71 (MQ=255) ccATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTa > 1:717175/1‑71 (MQ=255) cATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTac < 1:3696802/71‑1 (MQ=255) atgccgaccgGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTacc > 1:3217624/4‑71 (MQ=255) atgccgaccgGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTacc > 1:242734/4‑71 (MQ=255) tACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAg > 1:2835182/1‑57 (MQ=255) tACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTacca > 1:1057730/1‑71 (MQ=255) gccgaccgGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGa < 1:3117168/58‑1 (MQ=255) accgaccgGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTaccac > 1:3530167/1‑71 (MQ=255) accgaccgGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTaccac > 1:1268200/1‑71 (MQ=255) accgaccgGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTacca > 1:2071780/1‑70 (MQ=255) ccgaccgGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTaccac > 1:2396244/1‑70 (MQ=255) cgaccgGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTaccacc > 1:2415730/1‑70 (MQ=255) gaccgGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTAccgcca > 1:401388/1‑70 (MQ=255) accgGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCg < 1:3857586/54‑1 (MQ=255) accgGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCaaa > 1:1992963/1‑71 (MQ=255) cgGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACt < 1:577221/71‑1 (MQ=255) cgGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACt < 1:2441658/71‑1 (MQ=255) cgGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACt < 1:3477962/71‑1 (MQ=255) gggCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTg < 1:2890421/71‑1 (MQ=255) ggCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTacca > 1:705830/1‑62 (MQ=255) ggCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTacca > 1:2087469/1‑62 (MQ=255) ggCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTgc < 1:1629995/71‑1 (MQ=255) aGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTAtt < 1:267602/54‑1 (MQ=255) aGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTAtt < 1:2946039/54‑1 (MQ=255) aGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCa > 1:2787290/1‑71 (MQ=255) aGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTgcgcg > 1:2597170/1‑70 (MQ=255) aTGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTc < 1:1061449/37‑1 (MQ=255) tGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCa < 1:2837449/71‑1 (MQ=255) cgcCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGa > 1:2380973/1‑71 (MQ=255) gcCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAg < 1:3144837/43‑1 (MQ=255) gcCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAg < 1:691562/43‑1 (MQ=255) gcCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAg < 1:562541/43‑1 (MQ=255) gcCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAg < 1:2926827/43‑1 (MQ=255) ccGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCa < 1:472444/63‑1 (MQ=255) ccGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTAtt < 1:2301721/46‑1 (MQ=255) ccGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTAtt < 1:36690/46‑1 (MQ=255) ccGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGAcc < 1:2259648/71‑1 (MQ=255) ccGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGAcc < 1:322860/71‑1 (MQ=255) ccGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGAc > 1:3107929/1‑70 (MQ=255) cGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGAcc > 1:2369732/1‑70 (MQ=255) cGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGAcc > 1:73788/1‑70 (MQ=255) cGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGAcc < 1:2094105/70‑1 (MQ=255) tGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACt > 1:186372/1‑55 (MQ=255) gACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTaccacc < 1:3492478/49‑1 (MQ=255) aCGTGGCAGGCGAATCACGAGGATGTCGTGAGCGAAGTATTTACCACCAAAc > 1:117988/1‑52 (MQ=255) gTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGAttt > 1:1204794/1‑71 (MQ=255) tGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGtttct < 1:3538794/71‑3 (MQ=255) ggCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTg > 1:3915066/1‑50 (MQ=255) gCAGGCGAATCACGCGGATGTCGTGAGCGAAGTAtt < 1:3942081/36‑1 (MQ=255) gCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGTTTCTgg > 1:197621/1‑70 (MQ=255) gCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGTTTCTgg > 1:3748278/1‑70 (MQ=255) aGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTg < 1:2654428/68‑1 (MQ=255) aGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTg < 1:1729927/68‑1 (MQ=255) gCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCAcc > 1:3176725/1‑51 (MQ=255) gAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACt < 1:2200366/42‑1 (MQ=255) gAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCAcc < 1:2672173/49‑1 (MQ=255) aaTCACGCGGATGTCGTGAGCGAAGTATTTACCACCaaa > 1:404564/1‑39 (MQ=255) aaTCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGa < 1:2636938/71‑1 (MQ=255) aaTCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCGTCGa < 1:1510792/71‑1 (MQ=255) aTCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGAt > 1:2149742/1‑71 (MQ=255) tCACGCGGATGTCGTGAGCGAAGTATTTACCACCTAACTGCGCACCCAGACCAAGATTTTGCGCTTCGAt > 1:1224255/1‑70 (MQ=255) cgGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTc > 1:255338/1‑62 (MQ=255) | TCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGAT > minE/1015107‑1015237 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |