| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,015,199 | A→G | 26.4% | G290G (GGT→GGC) | fumA ← | aerobic Class I fumarate hydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,015,199 | 0 | A | G | 26.4% | 88.3 / 32.8 | 53 | G290G (GGT→GGC) | fumA | aerobic Class I fumarate hydratase |
| Reads supporting (aligned to +/- strand): ref base A (20/19); new base G (5/9); total (25/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.65e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.23e-01 | |||||||||||
TACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCG > minE/1015129‑1015265 | tACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTacca > 1:1057730/1‑71 (MQ=255) accgaccgGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTaccac > 1:3530167/1‑71 (MQ=255) accgaccgGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTaccac > 1:1268200/1‑71 (MQ=255) accgaccgGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTacca > 1:2071780/1‑70 (MQ=255) ccgaccgGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTaccac > 1:2396244/1‑70 (MQ=255) cgaccgGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTaccacc > 1:2415730/1‑70 (MQ=255) gaccgGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTAccgcca > 1:401388/1‑70 (MQ=255) accgGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCaaa > 1:1992963/1‑71 (MQ=255) cgGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACt < 1:3477962/71‑1 (MQ=255) cgGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACt < 1:2441658/71‑1 (MQ=255) cgGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACt < 1:577221/71‑1 (MQ=255) gggCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTg < 1:2890421/71‑1 (MQ=255) ggCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTacca > 1:2087469/1‑62 (MQ=255) ggCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTacca > 1:705830/1‑62 (MQ=255) ggCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTgc < 1:1629995/71‑1 (MQ=255) aGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCa > 1:2787290/1‑71 (MQ=255) aGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTgcgcg > 1:2597170/1‑70 (MQ=255) tGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCa < 1:2837449/71‑1 (MQ=255) cgcCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGa > 1:2380973/1‑71 (MQ=255) ccGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCa < 1:472444/63‑1 (MQ=255) ccGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGAcc < 1:322860/71‑1 (MQ=255) ccGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGAcc < 1:2259648/71‑1 (MQ=255) ccGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGAc > 1:3107929/1‑70 (MQ=255) cGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGAcc > 1:73788/1‑70 (MQ=255) cGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGAcc > 1:2369732/1‑70 (MQ=255) cGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGAcc < 1:2094105/70‑1 (MQ=255) tGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACt > 1:186372/1‑55 (MQ=255) gACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTaccacc < 1:3492478/49‑1 (MQ=255) aCGTGGCAGGCGAATCACGAGGATGTCGTGAGCGAAGTATTTACCACCAAAc > 1:117988/1‑52 (MQ=255) gTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGAttt > 1:1204794/1‑71 (MQ=255) tGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGtttct < 1:3538794/71‑3 (MQ=255) ggCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTg > 1:3915066/1‑50 (MQ=255) gCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGTTTCTgg > 1:197621/1‑70 (MQ=255) gCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGTTTCTgg > 1:3748278/1‑70 (MQ=255) aGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTg < 1:2654428/68‑1 (MQ=255) aGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTg < 1:1729927/68‑1 (MQ=255) gCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCAcc > 1:3176725/1‑51 (MQ=255) gAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACt < 1:2200366/42‑1 (MQ=255) gAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCAcc < 1:2672173/49‑1 (MQ=255) aaTCACGCGGATGTCGTGAGCGAAGTATTTACCACCaaa > 1:404564/1‑39 (MQ=255) aaTCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGa < 1:2636938/71‑1 (MQ=255) aaTCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCGTCGa < 1:1510792/71‑1 (MQ=255) aTCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGAt > 1:2149742/1‑71 (MQ=255) tCACGCGGATGTCGTGAGCGAAGTATTTACCACCTAACTGCGCACCCAGACCAAGATTTTGCGCTTCGAt > 1:1224255/1‑70 (MQ=255) cgGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTc > 1:255338/1‑62 (MQ=255) tGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCt < 1:2850582/71‑1 (MQ=255) tCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCttt < 1:123056/71‑1 (MQ=255) tGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCg < 1:2444911/54‑1 (MQ=255) gAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTc > 1:1434402/1‑70 (MQ=255) gCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGTTTCTGGGCCTCTTCCAGCAGTTCCTGTTCCAg < 1:761127/71‑1 (MQ=255) aaGTATTTACCGCCAAACTGCGCGCCAAGACCGAGTTTCTgggc > 1:1193010/1‑44 (MQ=12) aaGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTc > 1:1230023/1‑71 (MQ=255) aGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTcc < 1:1825202/71‑1 (MQ=255) tATTTACCGCCAAACTGCGCGCCAAGACCGAGtttc < 1:3255009/36‑2 (MQ=12) tATTTACCGCCAAACTGCGCGCCAAGACCGAGtttc < 1:1917897/36‑2 (MQ=12) tATTTACCGCCAAACTGCGCGCCAAGACCGAGtttc < 1:45772/36‑2 (MQ=12) tATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCTCTTCGATCAGCAATTCTTTTTcc < 1:3576004/63‑1 (MQ=255) tATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCcac < 1:3951433/71‑1 (MQ=255) aTTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCcaca > 1:2323110/1‑71 (MQ=255) aTTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCcaca > 1:1578601/1‑71 (MQ=255) tttACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCtt < 1:3916735/56‑1 (MQ=255) tACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCa > 1:3248751/1‑60 (MQ=255) tACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATcg > 1:1425660/1‑71 (MQ=255) | TACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCG > minE/1015129‑1015265 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |