| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,015,220 | A→G | 23.6% | L283L (CTT→CTC) | fumA ← | aerobic Class I fumarate hydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,015,220 | 0 | A | G | 23.6% | 102.9 / 29.6 | 55 | L283L (CTT→CTC) | fumA | aerobic Class I fumarate hydratase |
| Reads supporting (aligned to +/- strand): ref base A (22/20); new base G (6/7); total (28/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.58e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.04e-01 | |||||||||||
GTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGC > minE/1015156‑1015283 | gTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGAttt > 1:1204794/1‑71 (MQ=255) tGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGtttct < 1:3538794/71‑3 (MQ=255) gCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGTTTCTgg > 1:3748278/1‑70 (MQ=255) gCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGTTTCTgg > 1:197621/1‑70 (MQ=255) aGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTg < 1:2654428/68‑1 (MQ=255) aGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTg < 1:1729927/68‑1 (MQ=255) aaTCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGa < 1:2636938/71‑1 (MQ=255) aaTCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCGTCGa < 1:1510792/71‑1 (MQ=255) aTCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGAt > 1:2149742/1‑71 (MQ=255) tCACGCGGATGTCGTGAGCGAAGTATTTACCACCTAACTGCGCACCCAGACCAAGATTTTGCGCTTCGAt > 1:1224255/1‑70 (MQ=255) cgGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTc > 1:255338/1‑62 (MQ=255) tGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCt < 1:2850582/71‑1 (MQ=255) tCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCttt < 1:123056/71‑1 (MQ=255) tGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCg < 1:2444911/54‑1 (MQ=255) gAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTc > 1:1434402/1‑70 (MQ=255) gCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGTTTCTGGGCCTCTTCCAGCAGTTCCTGTTCCAg < 1:761127/71‑1 (MQ=255) aaGTATTTACCGCCAAACTGCGCGCCAAGACCGAGTTTCTgggc > 1:1193010/1‑44 (MQ=12) aaGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTc > 1:1230023/1‑71 (MQ=255) aGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTcc < 1:1825202/71‑1 (MQ=255) tATTTACCGCCAAACTGCGCGCCAAGACCGAGtttc < 1:3255009/36‑2 (MQ=12) tATTTACCGCCAAACTGCGCGCCAAGACCGAGtttc < 1:45772/36‑2 (MQ=12) tATTTACCGCCAAACTGCGCGCCAAGACCGAGtttc < 1:1917897/36‑2 (MQ=12) tATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCTCTTCGATCAGCAATTCTTTTTcc < 1:3576004/63‑1 (MQ=255) tATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCcac < 1:3951433/71‑1 (MQ=255) aTTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCcaca > 1:1578601/1‑71 (MQ=255) aTTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCcaca > 1:2323110/1‑71 (MQ=255) tttACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCtt < 1:3916735/56‑1 (MQ=255) tACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCa > 1:3248751/1‑60 (MQ=255) tACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATcg > 1:1425660/1‑71 (MQ=255) gccaAACTGCGCGCCAAGACCGAGTTTCTGGGCCTCTTCCAGCAGTTCCTGTTCCAgc < 1:38180/57‑2 (MQ=255) ccaAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCttttt > 1:933603/1‑52 (MQ=255) ccaAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCttttt > 1:3846029/1‑52 (MQ=255) aaaCTGCGCGCCAAGACCGAGTTTCTGGGCCTCTTCCAGCAGTTCCTGTTCCAGCTGGACATCGCGGAACg < 1:1363336/71‑1 (MQ=255) aaaCTGCGCGCCAAGACCGAGTTTCTGGGCCTCTTCCAGCAGTTCCTGTTCCAGCTGGACATCGCGGAACg > 1:3231890/1‑71 (MQ=255) aaaCTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCt > 1:924361/1‑46 (MQ=255) aaCTGCGCGCCAAGACCGAGTTTCTGGGCCTCTTCCAGCAGTTCCTGTTCCAGCTGGACATCGCGGAACGc > 1:2134061/1‑71 (MQ=255) aCTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGcc > 1:772568/1‑71 (MQ=255) cTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTc > 1:1734605/1‑54 (MQ=255) cTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACAt < 1:572389/59‑1 (MQ=255) cTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACAt < 1:3433633/59‑1 (MQ=255) cTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCt > 1:1216288/1‑71 (MQ=255) tGCGCGCCAAGACCGAGTTTCTGGGCCTCTTCCAGCAGTTCCTGTTCCAGCTGGACATCGCGGAACGCCt > 1:31996/1‑70 (MQ=255) gcgcACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCttt > 1:2006461/1‑43 (MQ=255) gcACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGAcc < 1:164936/71‑1 (MQ=255) gcACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGAcc < 1:151129/71‑1 (MQ=255) cACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCg < 1:3950693/71‑1 (MQ=255) cACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCg < 1:2768556/71‑1 (MQ=255) aCCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACAt < 1:3234393/53‑1 (MQ=255) ccAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGc > 1:1908612/1‑71 (MQ=255) cAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCg < 1:2190500/67‑1 (MQ=255) aGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTg < 1:1935804/62‑1 (MQ=255) gACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCca > 1:2463939/1‑45 (MQ=255) gACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCca > 1:2516226/1‑45 (MQ=255) gACAAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCca > 1:1728732/1‑45 (MQ=255) aCCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGa > 1:1696140/1‑61 (MQ=255) | GTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGC > minE/1015156‑1015283 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |