| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,015,236 | A→T | 8.8% | I278N (ATC→AAC) ‡ | fumA ← | aerobic Class I fumarate hydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,015,236 | 0 | A | T | 8.8% | 183.8 / 9.3 | 68 | I278N (ATC→AAC) ‡ | fumA | aerobic Class I fumarate hydratase |
| Reads supporting (aligned to +/- strand): ref base A (34/28); new base T (3/3); total (37/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01 | |||||||||||
AATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGT > minE/1015166‑1015304 | aaTCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGa < 1:2636938/71‑1 (MQ=255)aaTCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCGTCGa < 1:1510792/71‑1 (MQ=255) aTCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGAt > 1:2149742/1‑71 (MQ=255) tCACGCGGATGTCGTGAGCGAAGTATTTACCACCTAACTGCGCACCCAGACCAAGATTTTGCGCTTCGAt > 1:1224255/1‑70 (MQ=255) tGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCt < 1:2850582/71‑1 (MQ=255) tCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCttt < 1:123056/71‑1 (MQ=255) gAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTc > 1:1434402/1‑70 (MQ=255) gCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGTTTCTGGGCCTCTTCCAGCAGTTCCTGTTCCAg < 1:761127/71‑1 (MQ=255) aaGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTc > 1:1230023/1‑71 (MQ=255) aGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTcc < 1:1825202/71‑1 (MQ=255) tATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCTCTTCGATCAGCAATTCTTTTTcc < 1:3576004/63‑1 (MQ=255) tATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCcac < 1:3951433/71‑1 (MQ=255) aTTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCcaca > 1:1578601/1‑71 (MQ=255) aTTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCcaca > 1:2323110/1‑71 (MQ=255) tttACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCtt < 1:3916735/56‑1 (MQ=255) tACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCa > 1:3248751/1‑60 (MQ=255) tACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATcg > 1:1425660/1‑71 (MQ=255) gccaAACTGCGCGCCAAGACCGAGTTTCTGGGCCTCTTCCAGCAGTTCCTGTTCCAgc < 1:38180/57‑2 (MQ=255) ccaAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCttttt > 1:933603/1‑52 (MQ=255) ccaAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCttttt > 1:3846029/1‑52 (MQ=255) aaaCTGCGCGCCAAGACCGAGTTTCTGGGCCTCTTCCAGCAGTTCCTGTTCCAGCTGGACATCGCGGAACg > 1:3231890/1‑71 (MQ=255) aaaCTGCGCGCCAAGACCGAGTTTCTGGGCCTCTTCCAGCAGTTCCTGTTCCAGCTGGACATCGCGGAACg < 1:1363336/71‑1 (MQ=255) aaaCTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCt > 1:924361/1‑46 (MQ=255) aaCTGCGCGCCAAGACCGAGTTTCTGGGCCTCTTCCAGCAGTTCCTGTTCCAGCTGGACATCGCGGAACGc > 1:2134061/1‑71 (MQ=255) aCTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGcc > 1:772568/1‑71 (MQ=255) cTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTc > 1:1734605/1‑54 (MQ=255) cTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACAt < 1:572389/59‑1 (MQ=255) cTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACAt < 1:3433633/59‑1 (MQ=255) cTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCt > 1:1216288/1‑71 (MQ=255) tGCGCGCCAAGACCGAGTTTCTGGGCCTCTTCCAGCAGTTCCTGTTCCAGCTGGACATCGCGGAACGCCt > 1:31996/1‑70 (MQ=255) gcgcACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCttt > 1:2006461/1‑43 (MQ=255) gcACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGAcc < 1:164936/71‑1 (MQ=255) gcACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGAcc < 1:151129/71‑1 (MQ=255) cACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCg < 1:3950693/71‑1 (MQ=255) cACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCg < 1:2768556/71‑1 (MQ=255) aCCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACAt < 1:3234393/53‑1 (MQ=255) ccAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGc > 1:1908612/1‑71 (MQ=255) cAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCg < 1:2190500/67‑1 (MQ=255) aGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTg < 1:1935804/62‑1 (MQ=255) gACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCca > 1:2463939/1‑45 (MQ=255) gACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCca > 1:2516226/1‑45 (MQ=255) gACAAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCca > 1:1728732/1‑45 (MQ=255) aCCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGa > 1:1696140/1‑61 (MQ=255) aGATTTTGCGCTTCGATCAGCAATTCTTTTTACAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTccc > 1:3387117/1‑71 (MQ=255) gATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGa > 1:1219349/1‑56 (MQ=255) ttttGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGaa > 1:2285161/1‑47 (MQ=255) ttttGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTc > 1:1230565/1‑71 (MQ=255) tttGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTg < 1:920588/52‑1 (MQ=255) tttGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTg < 1:3274110/52‑1 (MQ=255) ttGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTcc < 1:691656/70‑1 (MQ=255) tGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGa < 1:2296884/43‑1 (MQ=255) tGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCg < 1:3796645/70‑1 (MQ=255) tGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCg < 1:1863658/70‑1 (MQ=255) gcgcTTCGATCAGCAATTCTTTTTCCAGTTCCACATcg < 1:617746/38‑1 (MQ=255) cgcTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGtt < 1:3467956/70‑1 (MQ=255) gcTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCg < 1:3005636/67‑1 (MQ=255) gcTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTgg > 1:1797452/1‑71 (MQ=255) cTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGa > 1:634963/1‑39 (MQ=255) cTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCa > 1:395392/1‑56 (MQ=255) cTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCa > 1:2385587/1‑56 (MQ=255) cTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCa > 1:137271/1‑56 (MQ=255) cTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGc < 1:279154/71‑1 (MQ=255) ttCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGAc < 1:1625035/47‑1 (MQ=255) ttCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTgg > 1:262112/1‑69 (MQ=255) cGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTg > 1:2446395/1‑49 (MQ=255) cGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGt > 1:1635787/1‑71 (MQ=255) cGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGt > 1:638593/1‑71 (MQ=255) cGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGt > 1:1567985/1‑71 (MQ=255) gATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGt < 1:1912878/70‑1 (MQ=255) gATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGt < 1:1938763/70‑1 (MQ=255) aTCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTgg < 1:479704/65‑1 (MQ=255) | AATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGT > minE/1015166‑1015304 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |