| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,015,280 | G→A | 11.9% | H263H (CAC→CAT) | fumA ← | aerobic Class I fumarate hydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,015,280 | 0 | G | A | 11.9% | 157.6 / 10.5 | 59 | H263H (CAC→CAT) | fumA | aerobic Class I fumarate hydratase |
| Reads supporting (aligned to +/- strand): ref base G (24/27); new base A (2/5); total (26/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.42e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.82e-01 | |||||||||||
CACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCAGTTTCACCGTTTTAAGGTT > minE/1015210‑1015348 | cACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCg < 1:2768556/71‑1 (MQ=255)cACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCg < 1:3950693/71‑1 (MQ=255) ccAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGc > 1:1908612/1‑71 (MQ=255) cAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCg < 1:2190500/67‑1 (MQ=255) aGATTTTGCGCTTCGATCAGCAATTCTTTTTACAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTccc > 1:3387117/1‑71 (MQ=255) ttttGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTc > 1:1230565/1‑71 (MQ=255) ttGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTcc < 1:691656/70‑1 (MQ=255) tGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCg < 1:1863658/70‑1 (MQ=255) tGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCg < 1:3796645/70‑1 (MQ=255) cgcTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGtt < 1:3467956/70‑1 (MQ=255) gcTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCg < 1:3005636/67‑1 (MQ=255) gcTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTgg > 1:1797452/1‑71 (MQ=255) cTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCa > 1:2385587/1‑56 (MQ=255) cTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCa > 1:137271/1‑56 (MQ=255) cTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCa > 1:395392/1‑56 (MQ=255) cTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGc < 1:279154/71‑1 (MQ=255) ttCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTgg > 1:262112/1‑69 (MQ=255) cGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTg > 1:2446395/1‑49 (MQ=255) cGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGt > 1:1635787/1‑71 (MQ=255) cGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGt > 1:1567985/1‑71 (MQ=255) cGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGt > 1:638593/1‑71 (MQ=255) gATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGt < 1:1938763/70‑1 (MQ=255) gATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGt < 1:1912878/70‑1 (MQ=255) aTCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTgg < 1:479704/65‑1 (MQ=255) cagcagTTCCTGTTCCAGCTGGACATCGCGGAACGCCTGACCATGTTCGTTCCCTTCCGTCGGCAGTtcat < 1:646300/71‑1 (MQ=255) cAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTtcat > 1:1757373/1‑71 (MQ=255) cAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTtca > 1:321642/1‑70 (MQ=255) gCAGTTCCTGTTCCAGCTGGACATCGCGGAACGCCTGACCATGTTCGTTCCCTTCCGTCGGCAGTtcatcg < 1:3297527/71‑2 (MQ=255) gCAGTTCCTGTTCCAGCTGGACATCGCGGAACGCCTGACCATGTTCGTTCCCTTCCGTCGGCAGTtca < 1:3419707/68‑1 (MQ=255) gCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTtcatca < 1:1664489/71‑1 (MQ=255) cAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTTcatcat < 1:290090/71‑1 (MQ=255) ttCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCAtt > 1:805861/1‑45 (MQ=255) tCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCtt > 1:3218016/1‑49 (MQ=255) tCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTtca > 1:1262810/1‑63 (MQ=255) cTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTTCATCATAg > 1:3740127/1‑68 (MQ=255) tttttCCAGTTCCACATCGCGGAACGCCTGACCTTGCTCATTCCCTTCCGtt < 1:3130134/52‑1 (MQ=255) ttttCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTTCATCATAGTAttt > 1:2936579/1‑71 (MQ=255) tttCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGTCAGTTCATCATAGTAt < 1:3444093/68‑1 (MQ=255) gttCCAGCTGGACATCGCGGAACGCCTGACCATGTTc > 1:2890939/2‑37 (MQ=12) ttCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTc > 1:3805777/1‑45 (MQ=255) ttCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTTCATCATAGTATTTc < 1:3471748/70‑1 (MQ=255) ttCCAGCTGGACATCGCGGAACGCCTGACCATGTTCGTTCCCTTCCGTCGGCAGTtcat > 1:2544471/1‑59 (MQ=255) ttCCAGCTGGACATCGCGGAACGCCTGACCATGTTCGTTCCCTTCCGTCGGCAGTTCATCGTAATagtg < 1:1632628/69‑4 (MQ=255) tCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTTCATCATAGTATTTcg < 1:142764/70‑1 (MQ=255) tCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTTCATCATAGTATTTcg < 1:3540392/70‑1 (MQ=255) ccAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCGGTTg < 1:1109231/48‑1 (MQ=255) ccAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTg < 1:383607/48‑1 (MQ=255) ccAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTTCATCATAGTATTTcg < 1:1381048/69‑1 (MQ=255) cAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCgg < 1:1792455/71‑1 (MQ=255) cAGTTCCACATCGCGGAACGCCTGACCGT‑CTCATTCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCgg < 1:3403505/70‑1 (MQ=255) ccACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGc > 1:63942/1‑70 (MQ=255) caTCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCAGt < 1:1568943/71‑1 (MQ=255) tCGCGGAACGCCTGACCATGTTCGTTCCCTTCCGtc < 1:573673/36‑2 (MQ=25) gAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCAGTTTCACCg > 1:1320581/1‑71 (MQ=255) aCGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCGGaa > 1:1699660/1‑56 (MQ=255) cGCCTGACCGTTCTCATTCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCAGTTTCACCGttt < 1:2229574/71‑1 (MQ=255) cGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTTCATCATAGTATTTcgcg < 1:2148689/52‑1 (MQ=255) cGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCAGTTTCACCGttt < 1:3311719/71‑1 (MQ=255) cGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCAGTTTCACCGttt > 1:3836725/1‑71 (MQ=255) gCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCAGTTTCACCGtttt < 1:3181957/71‑1 (MQ=255) ccTGACCGTGCTCATTCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCAg < 1:105375/58‑1 (MQ=255) ccGTGCTCATTCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCAGTTTCACCGTTTTAAGGtt < 1:1105546/71‑1 (MQ=255) | CACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCAGTTTCACCGTTTTAAGGTT > minE/1015210‑1015348 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |