| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 218,157 | C→T | 15.9% | D48D (GAC→GAT) | yafF → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 218,157 | 0 | C | T | 15.9% | 292.4 / 39.1 | 119 | D48D (GAC→GAT) | yafF | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (51/49); new base T (5/14); total (56/63) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.74e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 119 total aligned reads displayed.
ATATTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAGTAATACCCCGACTCTCCCCG > minE/218090‑218222 | atatTTTGACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAg > 1:1397117/1‑70 (MQ=255)atatTTTGACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAg > 1:3891349/1‑70 (MQ=255)atatTTTGACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAg < 1:1122330/70‑1 (MQ=255) tatTTTGACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACa < 1:3529269/68‑1 (MQ=255) tatTTTGACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGa < 1:3083070/70‑1 (MQ=255) tatTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGa < 1:3439000/70‑1 (MQ=255) tatTTTAACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGATAGaa < 1:1368838/71‑1 (MQ=255) atTTTGACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATAGACAGaaa > 1:2105796/1‑71 (MQ=255) ttttGACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAAc > 1:1118617/1‑71 (MQ=255) ttttGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAAc < 1:1755981/71‑1 (MQ=255) ttGACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACt < 1:2634741/70‑1 (MQ=255) ttGACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTa > 1:3356191/1‑71 (MQ=255) ttGACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTa > 1:2226934/1‑71 (MQ=255) gACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTAc < 1:1429119/70‑1 (MQ=255) aCGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCt < 1:486881/71‑1 (MQ=255) gAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTg > 1:1157819/1‑70 (MQ=255) agaAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCa < 1:2567343/71‑1 (MQ=255) taaGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAg < 1:2192812/70‑1 (MQ=255) aGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGc < 1:3423784/64‑1 (MQ=255) aGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTc < 1:2304032/71‑1 (MQ=255) aGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTATCTGGCGTCAGTc < 1:2308547/71‑1 (MQ=255) ggTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACATAAACTACCTGGCGTCAGTcc < 1:3012776/71‑1 (MQ=255) gTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCt > 1:2977425/1‑71 (MQ=255) tATTCAAGGCAGGGTTAAGACGTAATATGCGAAAAGCAGCCATGGACAGAAACTATCTg < 1:1249339/59‑1 (MQ=255) tATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTATCTg < 1:915348/59‑1 (MQ=255) tATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTATCTGGCGTCAGTCCtt < 1:1822875/71‑1 (MQ=255) ttCAAGGCAGTGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTg < 1:1133043/70‑1 (MQ=255) ttCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGATAGAAACTATCt < 1:2825384/56‑1 (MQ=255) ttCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGATAGAAACTATCt < 1:2829873/56‑1 (MQ=255) ttCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTg < 1:3432835/70‑1 (MQ=255) ttCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGa < 1:1511386/48‑1 (MQ=255) ttCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGa < 1:3141568/48‑1 (MQ=255) ttCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGc < 1:2383706/71‑1 (MQ=255) ttCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGc < 1:1626290/71‑1 (MQ=255) tCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTcgcg > 1:5689/1‑59 (MQ=255) cAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCtt > 1:1861789/1‑67 (MQ=255) cAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAg > 1:1485514/1‑45 (MQ=255) cAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTTGCGTCAGTCCTTGCgg > 1:2889748/1‑71 (MQ=255) cAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCgg > 1:3418360/1‑71 (MQ=255) cAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTACgg > 1:2222874/1‑71 (MQ=255) cAAGGCA‑GGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTg > 1:225888/1‑54 (MQ=255) aaGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGaaa > 1:555751/1‑47 (MQ=255) aaGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGaaa > 1:1200662/1‑47 (MQ=255) aaGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTACggg > 1:2057439/1‑71 (MQ=255) aGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTAcc < 1:2795499/51‑1 (MQ=255) ggCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTat > 1:488574/1‑48 (MQ=255) ggCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTAc > 1:2202170/1‑49 (MQ=255) gCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAg > 1:1061903/1‑71 (MQ=255) cAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTGCGGGGAGc < 1:1069311/71‑1 (MQ=255) gggTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTACGGGGAGCgg < 1:1027957/71‑1 (MQ=255) ggTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTa > 1:713361/1‑43 (MQ=255) ggTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTATCTGGCGTCAGTCCTTGc < 1:1948180/61‑1 (MQ=255) ggTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGt < 1:2095755/55‑1 (MQ=255) ttAAGACGTAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTcgcg < 1:1745777/48‑1 (MQ=255) ttAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTATCTGGCGTCAGTCCTTGCGGGGAGCGGGCt > 1:3535584/1‑71 (MQ=255) ttAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCt < 1:1114153/71‑1 (MQ=255) ttAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCt < 1:2674328/71‑1 (MQ=255) ttAAGACG‑AAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGt < 1:3814885/48‑1 (MQ=39) tAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTACGGGTAGCGGGCtt > 1:1790473/1‑71 (MQ=255) aaGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTATCTGGCGTCAGTCCTTGCGGGGAGCGGGCttt > 1:1243038/1‑71 (MQ=255) aaGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTACGGGGAGCGGGCtt < 1:3607905/70‑1 (MQ=255) aGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTATCTGGCGTCAGTCCTTGCGGGGAGCGGGCtt < 1:2100372/69‑1 (MQ=255) aGACGTAAGATGCGAAAAGCAGCAATGGACAGAAACTACCTGGCGTCAGTcc > 1:1627727/1‑52 (MQ=255) gACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTATCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCg > 1:114188/1‑71 (MQ=255) gACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTATCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCg > 1:1700406/1‑71 (MQ=255) gACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCt < 1:887272/40‑1 (MQ=255) gACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTc < 1:371921/50‑1 (MQ=255) gACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGa < 1:2466753/60‑1 (MQ=255) cGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTgg > 1:2171587/1‑40 (MQ=255) cGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCttac < 1:3455665/53‑3 (MQ=255) gTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGc < 1:1968357/40‑1 (MQ=255) gTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCtt > 1:3102107/1‑50 (MQ=255) gTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCtt > 1:2046771/1‑50 (MQ=255) tAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTGCGGGGAGc > 1:560724/1‑58 (MQ=255) aaGATGCGAAAAGCAGCCATGGACAGAAACTATCTGGCGt > 1:1415825/1‑40 (MQ=255) aaGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGTGGAGCGGGCTTTCgtag > 1:3456885/1‑69 (MQ=255) aaGATCCCAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCATACgtagt > 1:2704951/1‑70 (MQ=255) aGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAGTaa > 1:2145273/1‑71 (MQ=255) aGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAGTaa < 1:1763557/71‑1 (MQ=255) gATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTGCGGGGAGCGGGCTTTc > 1:3837798/1‑63 (MQ=255) aTGCGAAAAGCAGCCATGGACAGAAACTATCTGGCGTCAGt > 1:689513/1‑41 (MQ=255) tGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGt > 1:2997168/1‑36 (MQ=255) tGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTcc < 1:1156780/42‑1 (MQ=255) gCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTc > 1:2038019/1‑60 (MQ=255) cGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCtt > 1:264636/1‑42 (MQ=255) cGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTACGGGGAGCGGGCTTTCgtaatc < 1:3786105/65‑4 (MQ=255) gAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAGTAATAccc < 1:2576615/70‑1 (MQ=255) aaGCAGCCATGGACAGAAACTACCTGGCGTCAGTcc > 1:1308844/1‑36 (MQ=255) ggcagcTATGGTTAGAAACTACCTGACGGCAGTCCTTGCGGGGAGCAGGCTTTCgtaa < 1:2911956/57‑2 (MQ=255) gcagcCATGGATAGAAACTATCTCGCGTCAGTCCtt < 1:2477675/36‑1 (MQ=37) gcagcCATGGATAGAAACTATCTCGCGTCAGTCCTTGCggg < 1:3587545/41‑1 (MQ=255) gcagcCATGGACAGAAACTACCTGGCGTCAGTCCTTGCg < 1:3156852/39‑1 (MQ=255) gcagcCATGGACAGAAACTACCTGGCGTCAGTCCTTGCg < 1:167262/39‑1 (MQ=255) cagcCATGAACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAGTAATACCCCGActct < 1:338180/71‑1 (MQ=255) agcCATGGATAGAAACTATCTCGCGTCAGTCCTTGCGGGGAGCGGGCTTTCgtaat < 1:2745131/56‑3 (MQ=255) aTGGACAGAAACTACCTGGCGTAAGTCCTTGCGGGGAGCGGGCTTTCGTAGTAATACCCCGACTCTCCCCg > 1:2602766/1‑71 (MQ=255) tGGATAGAAACTATCTCGCGTCAGTCCTTGCGGGGAg < 1:1398882/37‑1 (MQ=37) tGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCg < 1:1676202/47‑1 (MQ=255) tGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAGTAATACCCCGACTCTcccc > 1:904705/1‑69 (MQ=255) gACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCgg > 1:1173149/1‑38 (MQ=255) | ATATTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAGTAATACCCCGACTCTCCCCG > minE/218090‑218222 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |