| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,015,298 | T→C | 11.5% | P257P (CCA→CCG) | fumA ← | aerobic Class I fumarate hydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,015,298 | 0 | T | C | 11.5% | 157.1 / 11.0 | 61 | P257P (CCA→CCG) | fumA | aerobic Class I fumarate hydratase |
| Reads supporting (aligned to +/- strand): ref base T (22/32); new base C (3/4); total (25/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.42e-01 | |||||||||||
CGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCAGTTTCACCGTTTTAAGGTTCGTTTCTGCAGAAGTTCCAC > minE/1015229‑1015368 | cgcTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGtt < 1:3467956/70‑1 (MQ=255) gcTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTgg > 1:1797452/1‑71 (MQ=255) cTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGc < 1:279154/71‑1 (MQ=255) ttCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTgg > 1:262112/1‑69 (MQ=255) cGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGt > 1:638593/1‑71 (MQ=255) cGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGt > 1:1635787/1‑71 (MQ=255) cGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGt > 1:1567985/1‑71 (MQ=255) gATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGt < 1:1912878/70‑1 (MQ=255) gATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGt < 1:1938763/70‑1 (MQ=255) aTCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTgg < 1:479704/65‑1 (MQ=255) cagcagTTCCTGTTCCAGCTGGACATCGCGGAACGCCTGACCATGTTCGTTCCCTTCCGTCGGCAGTtcat < 1:646300/71‑1 (MQ=255) cAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTtcat > 1:1757373/1‑71 (MQ=255) cAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTtca > 1:321642/1‑70 (MQ=255) gCAGTTCCTGTTCCAGCTGGACATCGCGGAACGCCTGACCATGTTCGTTCCCTTCCGTCGGCAGTtcatcg < 1:3297527/71‑2 (MQ=255) gCAGTTCCTGTTCCAGCTGGACATCGCGGAACGCCTGACCATGTTCGTTCCCTTCCGTCGGCAGTtca < 1:3419707/68‑1 (MQ=255) gCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTtcatca < 1:1664489/71‑1 (MQ=255) cAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTTcatcat < 1:290090/71‑1 (MQ=255) tCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTtca > 1:1262810/1‑63 (MQ=255) cTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTTCATCATAg > 1:3740127/1‑68 (MQ=255) tttttCCAGTTCCACATCGCGGAACGCCTGACCTTGCTCATTCCCTTCCGtt < 1:3130134/52‑1 (MQ=255) ttttCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTTCATCATAGTAttt > 1:2936579/1‑71 (MQ=255) tttCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGTCAGTTCATCATAGTAt < 1:3444093/68‑1 (MQ=255) ttCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTTCATCATAGTATTTc < 1:3471748/70‑1 (MQ=255) ttCCAGCTGGACATCGCGGAACGCCTGACCATGTTCGTTCCCTTCCGTCGGCAGTtcat > 1:2544471/1‑59 (MQ=255) ttCCAGCTGGACATCGCGGAACGCCTGACCATGTTCGTTCCCTTCCGTCGGCAGTTCATCGTAATagtg < 1:1632628/69‑4 (MQ=255) tCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTTCATCATAGTATTTcg < 1:142764/70‑1 (MQ=255) tCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTTCATCATAGTATTTcg < 1:3540392/70‑1 (MQ=255) ccAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCGGTTg < 1:1109231/48‑1 (MQ=255) ccAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTg < 1:383607/48‑1 (MQ=255) ccAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTTCATCATAGTATTTcg < 1:1381048/69‑1 (MQ=255) cAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCgg < 1:1792455/71‑1 (MQ=255) cAGTTCCACATCGCGGAACGCCTGACCGT‑CTCATTCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCgg < 1:3403505/70‑1 (MQ=255) ccACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGc > 1:63942/1‑70 (MQ=255) caTCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCAGt < 1:1568943/71‑1 (MQ=255) gAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCAGTTTCACCg > 1:1320581/1‑71 (MQ=255) aCGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCGGaa > 1:1699660/1‑56 (MQ=255) cGCCTGACCGTTCTCATTCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCAGTTTCACCGttt < 1:2229574/71‑1 (MQ=255) cGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTTCATCATAGTATTTcgcg < 1:2148689/52‑1 (MQ=255) cGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCAGTTTCACCGttt > 1:3836725/1‑71 (MQ=255) cGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCAGTTTCACCGttt < 1:3311719/71‑1 (MQ=255) gCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCAGTTTCACCGtttt < 1:3181957/71‑1 (MQ=255) ccTGACCGTGCTCATTCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCAg < 1:105375/58‑1 (MQ=255) ccGTGCTCATTCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCAGTTTCACCGTTTTAAGGtt < 1:1105546/71‑1 (MQ=255) tGCTCATTCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGc < 1:1251879/47‑1 (MQ=255) tGCTCATTCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCAGTTTCACCGTTTTAAGGTTCGt < 1:561368/71‑1 (MQ=255) gCTCATTCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCAGTTTCACCGTTTTAAGGTTCGtt > 1:3727133/1‑71 (MQ=255) cTCATTCCCTTCCGTTGGCAGTTCATCATAGTATTTcgcg > 1:1970032/1‑40 (MQ=255) cTCATTCCCTTCCGTTGGCAGTTCATCATAGTATTTcgcg > 1:3797188/1‑40 (MQ=255) cTCATTCCCTTCCGTTGGCAGTTCATCATAGTATTTcgcg > 1:723388/1‑40 (MQ=255) cTCATTCCCTTCCGTTGGCAGGTCATCATAGTATTTCGCGGAAGCCAGTTTCACCGTTTTAAGGTTCGttt < 1:2197495/71‑1 (MQ=255) tcgttCCCTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTc > 1:1332389/4‑71 (MQ=255) tcgttCCCTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTc > 1:3909374/4‑71 (MQ=255) tCATTCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCAGttt < 1:3012355/50‑1 (MQ=255) aTTCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCAGTTTCACCGTTTTAAGGTTCGTTTc < 1:167899/69‑1 (MQ=255) ttCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCAGTTTCACCGTTTTAAGGTTCGTTTCTg > 1:2860197/1‑70 (MQ=255) cccTTCCGTTGGCAGTTCATCATAGTATTTCGCGGaa > 1:1215210/1‑37 (MQ=255) ccTTCCGTTGGCAGGTCATCATAGTATTTCGCGGAAGCCAGTTTCACCGTTTTAAGGTTCGttt > 1:2532486/1‑64 (MQ=255) ttCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCAGTTTCACCGTTTTAAGGTTCGTTTCTGCAg < 1:2010506/68‑1 (MQ=255) ttCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCAGTTTCACCGTTTTAAGGTTCGTTTCTGCAGAAg > 1:3420221/1‑71 (MQ=255) ccGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCAGTTTCACCGTTTTAAGGTTCGTTTCTGCAGAAGt < 1:2809015/70‑1 (MQ=255) cGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCAGTTTCACCGTTTTAAGGTTCGTTTCTGCAGAAGtt < 1:361565/70‑1 (MQ=255) cGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCAGTTTCACCGTTTTAAGGTTCGTTTCTGCAGAAGTTc < 1:3370886/71‑1 (MQ=255) gTTGGCAGTTCATCATAGTATTTCGCGGAAGCCAGTTTCACCGTTTTAAGGTTCGTTTCTGCAGAAGTTcc < 1:1300484/71‑1 (MQ=255) tGGCAGTTCATCATAGTATTTCGCGGAAGCCAGTTTCACCGTTTTAAGGTTCGTTTCTGc > 1:779978/1‑60 (MQ=255) tGGCAGTTCATCATAGTATTTCGCGGAAGCCAGTTTCACCGTTTTAAGGTTCGTTTCTGCAGAAGTTccac > 1:536662/1‑71 (MQ=255) | CGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCAGTTTCACCGTTTTAAGGTTCGTTTCTGCAGAAGTTCCAC > minE/1015229‑1015368 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |