| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,015,451 | C→G | 16.6% | G206G (GGG→GGC) | fumA ← | aerobic Class I fumarate hydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,015,451 | 0 | C | G | 16.6% | 123.8 / 16.0 | 54 | G206G (GGG→GGC) | fumA | aerobic Class I fumarate hydratase |
| Reads supporting (aligned to +/- strand): ref base C (25/20); new base G (2/7); total (27/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.42e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01 | |||||||||||
ATACGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGC > minE/1015388‑1015519 | aTACGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCg < 1:3422075/71‑1 (MQ=255) aCGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTccc < 1:2724711/64‑1 (MQ=255) cGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGt > 1:3333164/1‑69 (MQ=255) cGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGt > 1:2216414/1‑69 (MQ=255) ggCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCtt < 1:1545850/71‑1 (MQ=255) gccgccGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGc < 1:3165892/68‑1 (MQ=255) cgccgTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCtt < 1:2783463/68‑1 (MQ=255) cgTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGttt < 1:756110/71‑1 (MQ=255) gTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTc < 1:802988/71‑1 (MQ=255) tACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGt < 1:2957101/56‑1 (MQ=255) tACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTcc > 1:2133629/1‑71 (MQ=255) cAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTATCCTGAt < 1:1437798/71‑1 (MQ=255) aGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGa > 1:3924993/1‑69 (MQ=255) gCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGa > 1:3683308/1‑68 (MQ=255) gCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGa > 1:2826789/1‑68 (MQ=255) cGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATag < 1:961320/70‑1 (MQ=255) tacgcATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTa > 1:429865/3‑67 (MQ=255) tacgcATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAgaga < 1:1089364/69‑1 (MQ=255) gcgcATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCa > 1:1995983/1‑44 (MQ=255) gcgcATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAgaga > 1:2117039/1‑70 (MQ=255) gcATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCtt < 1:72174/51‑1 (MQ=255) gcATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCtt < 1:246406/51‑1 (MQ=255) cATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCtt < 1:2898833/50‑1 (MQ=255) aTCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGt > 1:773441/1‑71 (MQ=255) aTCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGt > 1:928590/1‑71 (MQ=255) aTCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGt > 1:1650343/1‑71 (MQ=255) ttttctCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTc < 1:1852119/53‑1 (MQ=255) tcttctCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTg < 1:451989/58‑1 (MQ=255) cttctCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTc > 1:2744265/1‑54 (MQ=255) cttctCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCt > 1:2457701/1‑71 (MQ=255) cttctCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCt > 1:947164/1‑71 (MQ=255) ttctCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCtt > 1:770460/1‑71 (MQ=255) ctcGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTg < 1:486537/70‑1 (MQ=255) ctcGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTg < 1:3394272/70‑1 (MQ=255) ctcAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGt > 1:1384873/1‑71 (MQ=255) tcAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGtt > 1:2938573/1‑71 (MQ=255) cAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCttt > 1:1855221/1‑43 (MQ=255) aaCCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTg > 1:109881/1‑71 (MQ=255) aCCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATa < 1:3360637/53‑1 (MQ=255) ggTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTg < 1:2346197/46‑1 (MQ=255) gTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTg < 1:1585827/61‑1 (MQ=255) aaTTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGttt > 1:1472822/1‑62 (MQ=255) aaTTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGttt > 1:2359568/1‑62 (MQ=255) gtttttCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAgccgc < 1:1590002/70‑3 (MQ=255) tttttCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAaccacc < 1:678444/71‑1 (MQ=255) ttttCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAgccgccg > 1:1557771/1‑70 (MQ=255) tttCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAaccaccac > 1:557730/1‑71 (MQ=255) ttCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTc < 1:3827191/51‑1 (MQ=255) ttCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTc < 1:3466461/51‑1 (MQ=255) ttCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAaccaccacc > 1:2378811/1‑71 (MQ=255) cAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCt < 1:1077067/70‑1 (MQ=255) cAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACg < 1:3932005/47‑1 (MQ=255) aGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATa < 1:2934391/38‑1 (MQ=255) ttCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGc > 1:349173/1‑71 (MQ=255) tCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGc > 1:3921911/1‑70 (MQ=255) | ATACGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGC > minE/1015388‑1015519 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |