Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,015,457 C→A 14.7% T204T (ACG→ACT fumA ← aerobic Class I fumarate hydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,015,4570CA14.7% 175.0 / 12.8 61T204T (ACG→ACTfumAaerobic Class I fumarate hydratase
Reads supporting (aligned to +/- strand):  ref base C (28/24);  new base A (2/7);  total (30/31)
Fisher's exact test for biased strand distribution p-value = 1.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.32e-01

ATACGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGA  >  minE/1015388‑1015527
                                                                     |                                                                      
aTACGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCg                                                                       <  1:3422075/71‑1 (MQ=255)
   cGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGt                                                                      >  1:2216414/1‑69 (MQ=255)
   cGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGt                                                                      >  1:3333164/1‑69 (MQ=255)
            ggCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCtt                                                           <  1:1545850/71‑1 (MQ=255)
             gccgccGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGc                                                             <  1:3165892/68‑1 (MQ=255)
               cgccgTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCtt                                                           <  1:2783463/68‑1 (MQ=255)
                  cgTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGttt                                                     <  1:756110/71‑1 (MQ=255)
                   gTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTc                                                    <  1:802988/71‑1 (MQ=255)
                    tACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGt                                                                  <  1:2957101/56‑1 (MQ=255)
                    tACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTcc                                                   >  1:2133629/1‑71 (MQ=255)
                        cAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTATCCTGAt                                               <  1:1437798/71‑1 (MQ=255)
                         aGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGa                                                >  1:3924993/1‑69 (MQ=255)
                          gCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGa                                                >  1:3683308/1‑68 (MQ=255)
                          gCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGa                                                >  1:2826789/1‑68 (MQ=255)
                           cGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATag                                             <  1:961320/70‑1 (MQ=255)
                             tacgcATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTa                                              >  1:429865/3‑67 (MQ=255)
                             tacgcATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAgaga                                          <  1:1089364/69‑1 (MQ=255)
                              gcgcATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCa                                                                    >  1:1995983/1‑44 (MQ=255)
                              gcgcATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAgaga                                          >  1:2117039/1‑70 (MQ=255)
                                gcATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCtt                                                           <  1:72174/51‑1 (MQ=255)
                                gcATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCtt                                                           <  1:246406/51‑1 (MQ=255)
                                 cATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCtt                                                           <  1:2898833/50‑1 (MQ=255)
                                  aTCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGt                                     >  1:773441/1‑71 (MQ=255)
                                  aTCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGt                                     >  1:1650343/1‑71 (MQ=255)
                                  aTCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGt                                     >  1:928590/1‑71 (MQ=255)
                                   ttttctCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTc                                                    <  1:1852119/53‑1 (MQ=255)
                                   tcttctCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTg                                                 <  1:451989/58‑1 (MQ=255)
                                    cttctCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTc                                                    >  1:2744265/1‑54 (MQ=255)
                                    cttctCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCt                                   >  1:2457701/1‑71 (MQ=255)
                                    cttctCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCt                                   >  1:947164/1‑71 (MQ=255)
                                     ttctCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCtt                                  >  1:770460/1‑71 (MQ=255)
                                       ctcGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTg                                 <  1:486537/70‑1 (MQ=255)
                                       ctcGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTg                                 <  1:3394272/70‑1 (MQ=255)
                                       ctcAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGt                                >  1:1384873/1‑71 (MQ=255)
                                        tcAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGtt                               >  1:2938573/1‑71 (MQ=255)
                                         cAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCttt                                                          >  1:1855221/1‑43 (MQ=255)
                                          aaCCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTg                             >  1:109881/1‑71 (MQ=255)
                                           aCCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATa                                              <  1:3360637/53‑1 (MQ=255)
                                               ggTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTg                                                 <  1:2346197/46‑1 (MQ=255)
                                                gTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTg                                 <  1:1585827/61‑1 (MQ=255)
                                                  aaTTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGttt                              >  1:2359568/1‑62 (MQ=255)
                                                  aaTTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGttt                              >  1:1472822/1‑62 (MQ=255)
                                                   gtttttCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAgccgc                    <  1:1590002/70‑3 (MQ=255)
                                                    tttttCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAaccacc                   <  1:678444/71‑1 (MQ=255)
                                                     ttttCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAgccgccg                  >  1:1557771/1‑70 (MQ=255)
                                                      tttCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAaccaccac                 >  1:557730/1‑71 (MQ=255)
                                                       ttCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTc                                    <  1:3827191/51‑1 (MQ=255)
                                                       ttCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTc                                    <  1:3466461/51‑1 (MQ=255)
                                                       ttCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAaccaccacc                >  1:2378811/1‑71 (MQ=255)
                                                         cAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCt               <  1:1077067/70‑1 (MQ=255)
                                                         cAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACg                                      <  1:3932005/47‑1 (MQ=255)
                                                          aGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATa                                              <  1:2934391/38‑1 (MQ=255)
                                                             ttCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGc          >  1:349173/1‑71 (MQ=255)
                                                              tCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGc          >  1:3921911/1‑70 (MQ=255)
                                                                cccGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGa        <  1:36554/70‑1 (MQ=255)
                                                                 ccGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTg                                 <  1:3096924/44‑1 (MQ=255)
                                                                 ccGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATa      >  1:1389115/1‑71 (MQ=255)
                                                                 ccGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATa      <  1:3506317/71‑1 (MQ=255)
                                                                  cGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCg                          >  1:736124/1‑50 (MQ=255)
                                                                   ggCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATa      >  1:172658/1‑69 (MQ=255)
                                                                   ggCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACa    <  1:90233/71‑1 (MQ=255)
                                                                     cGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACaga  <  1:2916157/71‑1 (MQ=255)
                                                                     cGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACa    >  1:1100380/1‑69 (MQ=255)
                                                                     |                                                                      
ATACGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGA  >  minE/1015388‑1015527

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: