| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,015,511 | A→G | 8.7% | G186G (GGT→GGC) | fumA ← | aerobic Class I fumarate hydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,015,511 | 0 | A | G | 8.7% | 154.7 / 5.4 | 57 | G186G (GGT→GGC) | fumA | aerobic Class I fumarate hydratase |
| Reads supporting (aligned to +/- strand): ref base A (22/30); new base G (3/2); total (25/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.45e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.19e-01 | |||||||||||
TTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTATTCGTCGCCATCAACGGCATAAAGATCGATCTGCGCTGGCAGAT > minE/1015442‑1015581 | tttCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAaccaccac > 1:557730/1‑71 (MQ=255) ttCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAaccaccacc > 1:2378811/1‑71 (MQ=255) cAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCt < 1:1077067/70‑1 (MQ=255) ttCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGc > 1:349173/1‑71 (MQ=255) tCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGc > 1:3921911/1‑70 (MQ=255) cccGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGa < 1:36554/70‑1 (MQ=255) ccGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATa > 1:1389115/1‑71 (MQ=255) ccGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATa < 1:3506317/71‑1 (MQ=255) ggCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATa > 1:172658/1‑69 (MQ=255) ggCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACa < 1:90233/71‑1 (MQ=255) cGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACaga < 1:2916157/71‑1 (MQ=255) cGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACa > 1:1100380/1‑69 (MQ=255) tCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAgg < 1:2600837/71‑1 (MQ=255) tCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAgg < 1:3298385/71‑1 (MQ=255) aGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGaa > 1:1298969/1‑71 (MQ=255) aGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGaa > 1:1375379/1‑71 (MQ=255) aGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGaa < 1:1532209/71‑1 (MQ=255) aGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGaa > 1:1823738/1‑71 (MQ=255) tAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAAtt > 1:1573660/1‑71 (MQ=255) tAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAAt < 1:2676835/70‑1 (MQ=255) cGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTg > 1:1235750/1‑70 (MQ=255) cGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGt < 1:1777358/71‑1 (MQ=255) ttGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTATtc > 1:3679953/1‑71 (MQ=255) tGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTAt < 1:1847334/68‑1 (MQ=255) aggTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGa > 1:3286151/2‑51 (MQ=255) ggTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTATtcg < 1:267339/70‑1 (MQ=255) ggTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTATtcg < 1:1133749/70‑1 (MQ=255) gTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACtcatc > 1:3637666/1‑71 (MQ=255) gTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACtcatc > 1:91304/1‑71 (MQ=255) ccTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGcc < 1:2662379/70‑1 (MQ=255) ccTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTATTCGTCGcc < 1:3210899/70‑1 (MQ=255) ccTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTATTCGTCGCCa > 1:2124460/1‑71 (MQ=255) aTAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACag < 1:3437939/46‑1 (MQ=255) aTAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTATTCGTCGCCATCaa < 1:1264696/71‑1 (MQ=255) tAGAGATACGTCTTGTTTGCCGAACCACCACCttt < 1:2733750/35‑1 (MQ=255) agaTACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTATTCGTCGCCATCAACgg > 1:1848101/1‑70 (MQ=255) aTACGTCTTTTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTg < 1:2631744/49‑1 (MQ=255) tACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTATTCGTCGCCATCAACGGCATa < 1:3595424/71‑1 (MQ=255) aCGTCTTGTTTGCCGAACCACCACCTTTGGCGATAc < 1:2608746/36‑1 (MQ=255) aCGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGt > 1:2922014/1‑48 (MQ=255) gttttgttgGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCATCTACCGCGTACAg > 1:3328039/4‑71 (MQ=255) gTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTATTCGTCGCCATCAACGGCATa > 1:3798869/1‑68 (MQ=255) gTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTATTCGTCGCCATCAACGGCATa > 1:1096192/1‑68 (MQ=255) gTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTATTCGTCGCCATCAACGGCATAAAg < 1:2973055/71‑1 (MQ=255) gTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTATTCGTCGCCATCAACGGCATAAAg < 1:153561/71‑1 (MQ=255) tCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAAttt > 1:1492585/1‑43 (MQ=255) tCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTATTCGTCGcc < 1:3378794/55‑1 (MQ=255) tCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTATTCGTCGCCATCAACGGCATa < 1:1413470/67‑1 (MQ=255) ttGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTATTCGTCGCCATCAACgg > 1:3015595/1‑61 (MQ=255) gTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTg < 1:349614/40‑1 (MQ=255) gTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTATtc > 1:2403403/1‑45 (MQ=255) gTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTATTCGTCGCCATCAACGGCATAAAgatcg < 1:2793371/70‑1 (MQ=255) gTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTATTCGTCGCCATCAACGGCATAAAgatcg < 1:2024141/70‑1 (MQ=255) gCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTATTCGTCGCCATCAACGGCATAAAGATCGATCt < 1:54513/70‑1 (MQ=255) gCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTATTCGTCGCCATCAACGGCATAAAGATCGATCt < 1:3102309/70‑1 (MQ=255) gCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTATTCGTCGCCATCAACGGCATAAAGATCGATCt < 1:3737059/70‑1 (MQ=255) gAACCACCACCTTTGGCGATACAGAGGAATTTGTATTCGTCGCCATCAACGGCATAAAGATCGATCTgcgc > 1:2023856/1‑71 (MQ=255) aaCCACCACCTTTGGCGATACAGAGGTATTTGTATTCGTCGCCATCAACGGCATAAAGATCGATCTGCGCt < 1:931067/71‑1 (MQ=255) aaCCACCACCTTTGGCGATACAGAGGAATTTGTATTCGTCGCCATCAACGGCATAAAGATCGATCTGCGCt > 1:1308490/1‑71 (MQ=255) ccaccaccTTTGGCGATACAGAGGAATTTGTATtcgt > 1:2990294/1‑37 (MQ=255) ccaccaccTTTGGCGATACAGAGGAATTTGTATTCGTCGCCATCAACGGCATAAAGATCGATCTGCGCTgg > 1:1422564/1‑71 (MQ=255) caccaccTTTGGCGATACAGAGGAATTTGTATTCGTCGCCATCAACGGCATAAAGATCGATCTGCGCTGGc < 1:3881489/71‑1 (MQ=255) caccTTTGGCGATACAGAGGAATTTGTATTCGTCGCCATCAACGGCATAAAGATCGATCTGCGCTGGCAGa < 1:2109692/71‑1 (MQ=255) caccTTTGGCGATACAGAGGAATTTGTATTCGTCGCCATCAACGGCATAAAGATCGATCTGCGCTGGCAGa < 1:134595/71‑1 (MQ=255) accTTTGGCGATACAGAGGAATTTGTATTCGTCGCCATCAACGGCATAAAGATCGATCTgc > 1:2656308/1‑61 (MQ=255) accTTTGGCGATACAGAGGAATTTGTATTCGTCGCCATCAACGGCATAAAGATCGATCTGCGCTGGCAGAt > 1:3714698/1‑71 (MQ=255) | TTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACAGAGGAATTTGTATTCGTCGCCATCAACGGCATAAAGATCGATCTGCGCTGGCAGAT > minE/1015442‑1015581 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |