| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,015,592 | A→G | 14.3% | N159N (AAT→AAC) | fumA ← | aerobic Class I fumarate hydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,015,592 | 0 | A | G | 14.3% | 81.7 / 6.8 | 35 | N159N (AAT→AAC) | fumA | aerobic Class I fumarate hydratase |
| Reads supporting (aligned to +/- strand): ref base A (17/13); new base G (3/2); total (20/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 | |||||||||||
GAGGAATTTGTATTCGTCGCCATCAACGGCATAAAGATCGATCTGCGCTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAG > minE/1015526‑1015656 | gagGAATTTGTATTCGTCGCCATCAACGGCATAAAGATCGATCTGCGCTGGCAGATTGGTGCCGGCATTc < 1:912103/70‑1 (MQ=255) tcgtcgCCATCAACGGCATAAAGATCGATCTGCGCTGGCAGATTGGTGCCGGTATTCACTTCTTTATACa > 1:3839346/1‑70 (MQ=255) cgCCATCAACGGCATAAAGATCGATCTGCGCTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATc > 1:1805029/1‑70 (MQ=255) gCCATCAACGGCATAAAGATCGATCTGCGCTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCa < 1:3346520/71‑1 (MQ=255) cATCAACGGCATAAAGATCGATCTGCGCTGGCAGATTGGTGCCGGTa < 1:780447/47‑1 (MQ=255) cATCAACGGCATAAAGATCGATCTGCGCTGGCAGATTGGTGCCGGTa < 1:711158/47‑1 (MQ=255) cATCAACGGCATAAAGATCGATCTGCGCTGGCAGATTGGTGCCGGTa < 1:2981333/47‑1 (MQ=255) cATCAACGGCATAAAGATCGATCTGCGCTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAgc > 1:1081303/1‑71 (MQ=255) aTCAACGGCATAAAGATCGATCTGCGCTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAgcg > 1:1238325/1‑71 (MQ=255) cGGCATAAAGATCGATCTGCGCTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGcgcg > 1:881494/1‑71 (MQ=255) cGGCATAAAGATCGATCTGCGCTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGcgcg < 1:2255348/71‑1 (MQ=255) aTAAAGATCGATCTGCGCTGGCAGATTGGTGCCGGTAtt < 1:3110824/39‑1 (MQ=255) tAAAGATCGATCTGCGCTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGtttt > 1:1360761/1‑70 (MQ=255) aaaGATCGATCTGCGCTGGCAGATTGGTGCCGGTATTCACTTCttt > 1:1790791/1‑46 (MQ=255) aaaGATCGATCTGCGCTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGc > 1:2444147/1‑71 (MQ=255) aaaGATCGATCTGCGCTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGc > 1:2697727/1‑71 (MQ=255) aaGATCGATCTGCGCTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAg > 1:612707/1‑57 (MQ=255) atcgatcTGCGCTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGt < 1:1360564/71‑1 (MQ=255) tcgatcTGCGCTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTa < 1:3520849/71‑1 (MQ=255) cgatcTGCGCTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAg > 1:474873/1‑71 (MQ=255) cTGCGCTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAgcg > 1:3163368/1‑69 (MQ=255) cgcTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAgc < 1:3886365/65‑1 (MQ=255) cgcTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGa < 1:3519554/70‑1 (MQ=255) cgcTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGa < 1:3515551/70‑1 (MQ=255) tGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTa < 1:976736/70‑1 (MQ=255) gCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCt > 1:1024815/1‑71 (MQ=255) aGATTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTc > 1:1783645/1‑71 (MQ=255) aTTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTg > 1:2543854/1‑48 (MQ=255) aTTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCg > 1:1711911/1‑50 (MQ=255) aTTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGa > 1:2169316/1‑51 (MQ=255) tagTGCCGGTGTTGACCTCTTTGTAGATGTCCAGCGCCGCATTCTGTGAATAGCGCAGGTTATCTTCGata > 1:2766314/3‑71 (MQ=255) tagTGCCGGTGTTGACCTCTTTGTACATGTCCAGCGCCGCATTCTGTGAATAGCGCAGGTTATCTTCGata > 1:1925298/3‑71 (MQ=255) gTGCCGGTGTTGACCTCTTTGTACATGTCCAGCGCCGCATTCTGTGAATAGCGCAGGTTATCTTCGatat < 1:3170624/70‑1 (MQ=255) gTGCCGGTGTTGACCTCTTTGTACATGTCCAGCGCCGCATTCTGTGAATAGCGCAGGTTATCTTCGatat < 1:1847255/70‑1 (MQ=255) gTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGc < 1:3806834/45‑1 (MQ=255) gTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGt < 1:773594/49‑1 (MQ=255) ccGGTGTTGACCTCTTTGTACATGTCCAGCGCCGCATTCTGTGAATAGCGCAGGTTATCTTCGAtatagg > 1:1684446/1‑68 (MQ=255) cGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTc < 1:3922098/61‑1 (MQ=255) aTTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTa < 1:3442951/52‑1 (MQ=255) | GAGGAATTTGTATTCGTCGCCATCAACGGCATAAAGATCGATCTGCGCTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAG > minE/1015526‑1015656 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |