| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 218,169 | G→C | 15.2% | L52L (CTG→CTC) | yafF → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 218,169 | 0 | G | C | 15.2% | 206.3 / 23.9 | 86 | L52L (CTG→CTC) | yafF | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (34/38); new base C (4/9); total (39/47) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.68e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.62e-01 | |||||||||||
GAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAGTAATACCCCGACTCTCCCCGTCCTTAAACACAAC > minE/218100‑218236 | gAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTg > 1:1157819/1‑70 (MQ=255) agaAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCa < 1:2567343/71‑1 (MQ=255) taaGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAg < 1:2192812/70‑1 (MQ=255) aGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGc < 1:3423784/64‑1 (MQ=255) aGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTc < 1:2304032/71‑1 (MQ=255) aGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTATCTGGCGTCAGTc < 1:2308547/71‑1 (MQ=255) ggTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACATAAACTACCTGGCGTCAGTcc < 1:3012776/71‑1 (MQ=255) gTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCt > 1:2977425/1‑71 (MQ=255) tATTCAAGGCAGGGTTAAGACGTAATATGCGAAAAGCAGCCATGGACAGAAACTATCTg < 1:1249339/59‑1 (MQ=255) tATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTATCTg < 1:915348/59‑1 (MQ=255) tATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTATCTGGCGTCAGTCCtt < 1:1822875/71‑1 (MQ=255) ttCAAGGCAGTGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTg < 1:1133043/70‑1 (MQ=255) ttCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTg < 1:3432835/70‑1 (MQ=255) ttCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGc < 1:2383706/71‑1 (MQ=255) ttCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGc < 1:1626290/71‑1 (MQ=255) tCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTcgcg > 1:5689/1‑59 (MQ=255) tCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTACg > 1:2276626/1‑71 (MQ=255) cAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCtt > 1:1861789/1‑67 (MQ=255) cAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTTGCGTCAGTCCTTGCgg > 1:2889748/1‑71 (MQ=255) cAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCgg > 1:3418360/1‑71 (MQ=255) cAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTACgg > 1:2222874/1‑71 (MQ=255) cAAGGCA‑GGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTg > 1:225888/1‑54 (MQ=255) aaGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTACggg > 1:2057439/1‑71 (MQ=255) gCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAg > 1:1061903/1‑71 (MQ=255) cAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTGCGGGGAGc < 1:1069311/71‑1 (MQ=255) gggTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTACGGGGAGCgg < 1:1027957/71‑1 (MQ=255) ggTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTATCTGGCGTCAGTCCTTGc < 1:1948180/61‑1 (MQ=255) ggTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGt < 1:2095755/55‑1 (MQ=255) ttAAGACGTAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTcgcg < 1:1745777/48‑1 (MQ=255) ttAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTATCTGGCGTCAGTCCTTGCGGGGAGCGGGCt > 1:3535584/1‑71 (MQ=255) ttAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCt < 1:1114153/71‑1 (MQ=255) ttAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCt < 1:2674328/71‑1 (MQ=255) ttAAGACG‑AAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGt < 1:3814885/48‑1 (MQ=39) tAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTACGGGTAGCGGGCtt > 1:1790473/1‑71 (MQ=255) aaGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTATCTGGCGTCAGTCCTTGCGGGGAGCGGGCttt > 1:1243038/1‑71 (MQ=255) aaGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTACGGGGAGCGGGCtt < 1:3607905/70‑1 (MQ=255) aGACGTAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCa > 1:2054281/1‑48 (MQ=255) aGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTATCTGGCGTCAGTCCTTGCGGGGAGCGGGCtt < 1:2100372/69‑1 (MQ=255) aGACGTAAGATGCGAAAAGCAGCAATGGACAGAAACTACCTGGCGTCAGTcc > 1:1627727/1‑52 (MQ=255) gACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTATCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCg > 1:114188/1‑71 (MQ=255) gACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTATCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCg > 1:1700406/1‑71 (MQ=255) gACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTc < 1:371921/50‑1 (MQ=255) gACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGa < 1:2466753/60‑1 (MQ=255) cGTAAGATGCGAAAAGCAGCCATGGACAGAAACTATCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCgt > 1:3072249/1‑70 (MQ=255) cGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTgg > 1:2171587/1‑40 (MQ=255) cGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCttac < 1:3455665/53‑3 (MQ=255) gTAAGATGCGAAAAGCAGCCATGGACAGAAACTATCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCgtaa > 1:1832434/1‑70 (MQ=255) gTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGc < 1:1968357/40‑1 (MQ=255) gTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCtt > 1:2046771/1‑50 (MQ=255) gTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCtt > 1:3102107/1‑50 (MQ=255) gTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCtt > 1:3712929/1‑50 (MQ=255) tAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTGCGGGGAGc > 1:560724/1‑58 (MQ=255) aaGATGCGAAAAGCAGCCATGGACAGAAACTATCTGGCGt > 1:1415825/1‑40 (MQ=255) aaGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGTGGAGCGGGCTTTCgtag > 1:3456885/1‑69 (MQ=255) aaGATCCCAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCATACgtagt > 1:2704951/1‑70 (MQ=255) aGATGCGAAAAGCAGCCATGGACAGAAACTATCTGGCGTCAGTCCTTg < 1:2649544/48‑1 (MQ=255) aGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAGTaa > 1:2145273/1‑71 (MQ=255) aGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAGTaa < 1:1763557/71‑1 (MQ=255) gATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTGCGGGGAGCGGGCTTTc > 1:3837798/1‑63 (MQ=255) gATGCGAAAAGCAGCCATGGACAGAAACTATCTGGCGTCAGTCCTTGc < 1:219190/48‑1 (MQ=255) aTGCGAAAAGCAGCCATGGACAGAAACTATCTGGCGTCAGt > 1:689513/1‑41 (MQ=255) tGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGt > 1:2997168/1‑36 (MQ=255) tGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTcc < 1:1156780/42‑1 (MQ=255) gCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTc > 1:2038019/1‑60 (MQ=255) cGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCtt > 1:264636/1‑42 (MQ=255) cGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTACGGGGAGCGGGCTTTCgtaatc < 1:3786105/65‑4 (MQ=255) gAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAGTAATAccc < 1:2576615/70‑1 (MQ=255) aaaaGCAGCCATGGACAGAAACTACCTGGCGTCAGt < 1:1122428/36‑1 (MQ=255) aaaGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCgta > 1:2859295/1‑59 (MQ=255) aaGCAGCCATGGACAGAAACTACCTGGCGTCAGTcc > 1:1308844/1‑36 (MQ=255) ggcagcTATGGTTAGAAACTACCTGACGGCAGTCCTTGCGGGGAGCAGGCTTTCgtaa < 1:2911956/57‑2 (MQ=255) gcagcCATGGATAGAAACTATCTCGCGTCAGTCCtt < 1:2477675/36‑1 (MQ=37) gcagcCATGGATAGAAACTATCTCGCGTCAGTCCTTGCggg < 1:3587545/41‑1 (MQ=255) gcagcCATGGACAGAAACTACCTGGCGTCAGTCCTTGCg < 1:167262/39‑1 (MQ=255) gcagcCATGGACAGAAACTACCTGGCGTCAGTCCTTGCg < 1:3156852/39‑1 (MQ=255) cagcCATGAACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAGTAATACCCCGActct < 1:338180/71‑1 (MQ=255) agcCATGGATAGAAACTATCTCGCGTCAGTCCTTGCGGGGAGCGGGCTTTCgtaat < 1:1388687/56‑3 (MQ=255) agcCATGGATAGAAACTATCTCGCGTCAGTCCTTGCGGGGAGCGGGCTTTCgtaat < 1:2745131/56‑3 (MQ=255) ccATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCgtagta < 1:917290/55‑1 (MQ=255) aTGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGCGCGGGCTTTCGTAGTAAt > 1:2516777/1‑55 (MQ=255) aTGGACAGAAACTACCTGGCGTAAGTCCTTGCGGGGAGCGGGCTTTCGTAGTAATACCCCGACTCTCCCCg > 1:2602766/1‑71 (MQ=255) tGGATAGAAACTATCTCGCGTCAGTCCTTGCGGGGAg < 1:1398882/37‑1 (MQ=37) tGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCg < 1:1676202/47‑1 (MQ=255) tGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAGTAATACCCCGACTCTcccc > 1:904705/1‑69 (MQ=255) gACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCgg > 1:1173149/1‑38 (MQ=255) aaaCTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAGTAATACCCCGACTCTCCCCGTc > 1:2100415/1‑65 (MQ=255) aaaCTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAGTAATACCCCGACTCTCCCCGTCCTTaa < 1:2332396/70‑1 (MQ=255) aaCTACCTGGCGTCAGTCCTTACGGGGAGCGGGCttt < 1:1687960/37‑1 (MQ=255) aCTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAGTAATACCCCGACTCTCCCCGGCCTTAAaca > 1:856117/1‑71 (MQ=255) aCCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAGTAATAcccc < 1:2816994/47‑1 (MQ=255) aCCTGGCGTCAGTCCTTGCGGGGAGCGGGATTTCGTAGTaa > 1:1626310/1‑41 (MQ=255) ccTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAGTAATACCCCGACTCTCCCCGTCCTTAAACACAAc < 1:2506768/71‑1 (MQ=255) cTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAGTAATACCCCGACTCTCCCCGTCCTTAAACACaa < 1:3752986/69‑1 (MQ=255) | GAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAGTAATACCCCGACTCTCCCCGTCCTTAAACACAAC > minE/218100‑218236 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |