| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,015,631 | G→A | 24.4% | Y146Y (TAC→TAT) | fumA ← | aerobic Class I fumarate hydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,015,631 | 0 | G | A | 24.4% | 70.9 / 20.2 | 41 | Y146Y (TAC→TAT) | fumA | aerobic Class I fumarate hydratase |
| Reads supporting (aligned to +/- strand): ref base G (13/18); new base A (5/5); total (18/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.24e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATCGATCTGCGCTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCGCCAGCGCCGCTTCATCACCACCA > minE/1015562‑1015697 | atcgatcTGCGCTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGt < 1:1360564/71‑1 (MQ=255) tcgatcTGCGCTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTa < 1:3520849/71‑1 (MQ=255) cgatcTGCGCTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAg > 1:474873/1‑71 (MQ=255) cTGCGCTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAgcg > 1:3163368/1‑69 (MQ=255) cgcTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAgc < 1:3886365/65‑1 (MQ=255) cgcTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGa < 1:3519554/70‑1 (MQ=255) cgcTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGa < 1:3515551/70‑1 (MQ=255) tGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTa < 1:976736/70‑1 (MQ=255) gCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCt > 1:1024815/1‑71 (MQ=255) aGATTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTc > 1:1783645/1‑71 (MQ=255) tagTGCCGGTGTTGACCTCTTTGTAGATGTCCAGCGCCGCATTCTGTGAATAGCGCAGGTTATCTTCGata > 1:2766314/3‑71 (MQ=255) tagTGCCGGTGTTGACCTCTTTGTACATGTCCAGCGCCGCATTCTGTGAATAGCGCAGGTTATCTTCGata > 1:1925298/3‑71 (MQ=255) gTGCCGGTGTTGACCTCTTTGTACATGTCCAGCGCCGCATTCTGTGAATAGCGCAGGTTATCTTCGatat < 1:1847255/70‑1 (MQ=255) gTGCCGGTGTTGACCTCTTTGTACATGTCCAGCGCCGCATTCTGTGAATAGCGCAGGTTATCTTCGatat < 1:3170624/70‑1 (MQ=255) gTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGt < 1:773594/49‑1 (MQ=255) ccGGTGTTGACCTCTTTGTACATGTCCAGCGCCGCATTCTGTGAATAGCGCAGGTTATCTTCGAtatagg > 1:1684446/1‑68 (MQ=255) cGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTc < 1:3922098/61‑1 (MQ=255) aTTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTa < 1:3442951/52‑1 (MQ=255) ttCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTtata < 1:1956898/71‑1 (MQ=255) cACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCg > 1:3130321/1‑55 (MQ=255) cttcttTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAg > 1:306349/1‑68 (MQ=255) ttcttTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGAt > 1:3238234/1‑44 (MQ=255) tcttTGTACATGTCCAGCGCCGCATTCTGTGAATAGCGCAGGTTATCTTCGATATAGGTGTTATAGACGcc > 1:1308063/1‑71 (MQ=255) tcttTGTACATGTCCAGCGCCGCATTCTGTGAATAGCGCAGGTTATCTTCGATATAGGTGTTATAGACGcc > 1:3695163/1‑71 (MQ=255) tcttTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGatat < 1:3002265/55‑1 (MQ=255) tcttTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACAcc > 1:1078024/1‑71 (MQ=255) tttATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTa > 1:2820143/1‑43 (MQ=255) tgtaCATGTCCAGCGCCGCATTCTGTGAATAGCGCAGGTTATCTTCGATATAGGTGTTATAGACGcct < 1:228787/66‑2 (MQ=255) cATATCCAGCGGTGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCGCCa < 1:3592687/71‑1 (MQ=255) cATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCGCCa < 1:1279535/71‑1 (MQ=255) atatCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAg < 1:762288/49‑1 (MQ=255) tatCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCGCCAgc < 1:3927639/71‑1 (MQ=255) atCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGa > 1:2702300/1‑41 (MQ=255) tCCAGCGCCGCATTCTGTGAATAGCGCAGGTTATCTTCGATATAGGTGTTATAGACGcctt < 1:184665/61‑3 (MQ=255) gcgcGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCGCCAGCGCCGCTtc < 1:1202475/70‑1 (MQ=255) cgcaTTCTGTGAATAGCGCAGGTTATCTTCGATATAGGTGTTATAg < 1:3494254/46‑1 (MQ=255) gcgTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTtata < 1:1139577/44‑1 (MQ=255) tttGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAg > 1:912376/1‑41 (MQ=255) tttGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCGCCAg > 1:745678/1‑55 (MQ=255) gCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCGCCAGCGCCGCTTCATcaccacca > 1:3236232/1‑71 (MQ=255) gCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCGCCAGCGCCGCTTCATcaccac < 1:3645300/69‑1 (MQ=255) | ATCGATCTGCGCTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCGCCAGCGCCGCTTCATCACCACCA > minE/1015562‑1015697 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |